Restriction map for pF1KB7190
##################################
# NO CUT ENZYMES (  162 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AcsI        
AcyI        AfaI        AflII       AflIII      AgeI        
AhdI        Alw44I      ApaI        ApaLI       ApoI        
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AspI        AvaI        AvaII       AviII       
AvrII       BamHI       BbeI        BbrPI       BbvCI       
BclI        BfaI        BfrI        BglII       BlnI        
BlpI        Bpu1102I    BsaAI       BsaBI       BsaHI       
BsaWI       BseAI       BseRI       BsgI        BsiEI       
BsiWI       BsmBI       BsmFI       BsmI        BsoBI       
Bsp1407I    BspDI       BspEI       BspHI       BspLU11I    
BspMI       BsrBI       BsrDI       BsrGI       BssSI       
Bst1107I    BstAPI      BstBI       BstEII      BstPI       
BstXI       BstZ17I     CelII       ClaI        CpoI        
DraI        DraII       DraIII      DrdI        EagI        
Eam1105I    EarI        EclXI       Eco52I      EcoNI       
EcoO109I    EcoO65I     EcoRI       EcoRV       EcoT22I     
FauI        FbaI        FseI        FspI        Hin1I       
HindIII     HinfI       HpaI        KasI        KpnI        
Ksp632I     MaeI        MaeII       MamI        MfeI        
MluI        MroI        MseI        MunI        NaeI        
NarI        NcoI        NdeI        NgoMIV      NheI        
NlaIII      NotI        NruI        NsiI        NspI        
NspV        PacI        PaeR7I      PflFI       PinAI       
PleI        PmaCI       PmeI        PmlI        PpuMI       
PshAI       PshBI       Psp1406I    PstI        PvuI        
RcaI        RsaI        RsrII       SapI        SbfI        
ScaI        SexAI       SfaNI       SfiI        SfoI        
SfuI        SgrAI       SmaI        SmlI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
SwaI        TfiI        Tru9I       Tsp509I     TspRI       
Tth111I     XbaI        XcmI        XhoI        XmaCI       
XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   57 enzymes) #
###################################

AlwI          153  GATCC                GGATC(4/5)
AocI          364  CCTAAGG              CC^T[ACGT]AGG
Aor51HI        85  AGCGCT               AGC^GCT
AspHI         258  GAGCTC               G[AT]GC[AT]^C
BalI           62  TGGCCA               TGG^CCA
BanII         258  GAGCTC               G[GA]GC[CT]^C
BbsI          316  GAAGAC               GAAGAC(2/6)
BciVI         389  GTATCC               GTATCC(6/5)
BcnI            5  CCCGG                CC^[GC]GG
BglI           64  GCCACCAAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BmyI          258  GAGCTC               G[AGT]GC[ACT]^C
BpmI           89  CTCCAG               CTGGAG(16/14)
BpuAI         316  GAAGAC               GAAGAC(2/6)
BsaI          307  GGTCTC               GGTCTC(1/5)
BsiHKAI       258  GAGCTC               G[AT]GC[AT]^C
BsmAI         308  GTCTC                GTCTC(1/5)
Bsp1286I      258  GAGCTC               G[AGT]GC[ACT]^C
BsrFI          34  ACCGGC               [GA]^CCGG[CT]
BsrI           97  ACTGG                ACTGG(1/-1)
BssHII         76  GCGCGC               G^CGCGC
BstYI         152  AGATCC               [GA]^GATC[CT]
Bsu36I        364  CCTAAGG              CC^T[ACGT]AGG
Cfr10I         34  ACCGGC               [GA]^CCGG[CT]
Cfr13I          3  GGCCC                G^G[ACGT]CC
DpnII         153  GATC                 ^GATC
EaeI           62  TGGCCA               [CT]^GGCC[GA]
Eco47III       85  AGCGCT               AGC^GCT
Eco81I        364  CCTAAGG              CC^T[ACGT]AGG
EcoT14I        68  CCAAGG               C^C[AT][AT]GG
HgaI          384  GCGTC                GACGC(5/10)
HincII        171  GTCGAC               GT[CT]^[GA]AC
HindII        171  GTCGAC               GT[CT]^[GA]AC
HphI          267  GGTGA                GGTGA(8/7)
KspI           49  CCGCGG               CCGC^GG
MaeIII        352  GTCAC                ^GT[ACGT]AC
MboI          153  GATC                 ^GATC
MflI          152  AGATCC               [GA]^GATC[CT]
MluNI          62  TGGCCA               TGG^CCA
MscI           62  TGGCCA               TGG^CCA
MslI          354  CACTATTATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NciI            5  CCCGG                CC^[GC]GG
NdeII         153  GATC                 ^GATC
PflMI          91  CCAGCGACTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII          58  CAGCTG               CAG^CTG
SacI          258  GAGCTC               GAGCT^C
SacII          49  CCGCGG               CCGC^GG
SalI          171  GTCGAC               G^TCGAC
Sau3AI        153  GATC                 ^GATC
Sau96I          3  GGCCC                G^G[ACGT]CC
SfcI          123  CTACAG               C^T[GA][CT]AG
StuI          293  AGGCCT               AGG^CCT
StyI           68  CCAAGG               C^C[AT][AT]GG
TaqI          172  TCGA                 T^CGA
Tsp45I        352  GTCAC                ^GT[GC]AC
TthHB8I       172  TCGA                 T^CGA
Van91I         91  CCAGCGACTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII         152  AGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   18 enzymes) #
###################################

AccI           30  GTCTAC               GT^[AC][GT]AC
              171  GTCGAC              
AlwNI          92  CAGCGACTG            CAG[ACGT][ACGT][ACGT]^CTG
              205  CAGCGCCTG           
BanI           45  GGCACC               G^G[CT][GA]CC
              133  GGCACC              
BcgI          173  CGACTGAGCTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
               15  GCAGACAGCTCG        
BsiYI          91  CCAGCGACTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              255  CCAGAGCTCGG         
BslI           91  CCAGCGACTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              255  CCAGAGCTCGG         
BstF5I        186  CATCC                GGATG(2/0)
              372  CATCC               
BstNI         130  CCAGG                CC^[AT]GG
              210  CCTGG               
DdeI          176  CTGAG                C^T[ACGT]AG
              365  CTAAG               
EcoRII        130  CCAGG                ^CC[AT]GG
              210  CCTGG               
FokI          186  CATCC                GGATG(9/13)
              372  CATCC               
HapII           6  CCGG                 C^CGG
               35  CCGG                
HpaII           6  CCGG                 C^CGG
               35  CCGG                
MboII         108  GAAGA                GAAGA(8/7)
              316  GAAGA               
MspA1I         49  CCGCGG               C[AC]G^C[GT]G
               58  CAGCTG              
MspI            6  CCGG                 C^CGG
               35  CCGG                
MvaI          130  CCAGG                CC^[AT]GG
              210  CCTGG               
NlaIV          45  GGCACC               GG[ACGT]^[ACGT]CC
              133  GGCACC              


#########################################
# 3 OR MORE CUT ENZYMES (   21 enzymes) #
#########################################

AccII       AciI        AluI        BbvI        BsaJI       
BssKI       BstUI       Cac8I       CfoI        DsaI        
Fnu4HI      HaeII       HaeIII      HhaI        HinP1I      
ItaI        MnlI        MvnI        MwoI        ScrFI       
TseI        
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