Restriction map for pF1KB7190
 (Circular) (Six-base) MAPSORT of: pF1KB7190.seq  Check: 2373  from: 1  to: 3521



pF1KB7190  3521 bp

 With 182 enzymes: SgfI * 

                        June 20, 2008 10:26  ..

AccI GT'mk_AC

Cuts at:    139     280     559     139 
   Size:        141     279    3101

  Fragments arranged by size: 

                3101     279     141
 
Acc65I G'GTAC_C

Cuts at:    537     537 
   Size:       3521
 
AclI AA'CG_TT

Cuts at:   3361    3361 
   Size:       3521
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1346    1778    2525    2793    1346 
   Size:        432     747     268    2074

  Fragments arranged by size: 

                2074     747     432     268
 
AfeI AGC'GCT

Cuts at:    195     195 
   Size:       3521
 
AflIII A'CryG_T

Cuts at:    873    1977    2954     873 
   Size:       1104     977    1440

  Fragments arranged by size: 

                1440    1104     977
 
AgeI A'CCGG_T

Cuts at:   1909    1909 
   Size:       3521
 
AlwNI CAG_nnn'CTG

Cuts at:    205     318     826    2393     205 
   Size:        113     508    1567    1333

  Fragments arranged by size: 

                1567    1333     508     113
 
ApaLI G'TGCA_C

Cuts at:   2291    2291 
   Size:       3521
 
ApoI r'AATT_y

Cuts at:    525    2874    3130     525 
   Size:       2349     256     916

  Fragments arranged by size: 

                2349     916     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       3521
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3521
 
AvaI C'yCGr_G

Cuts at:    541     541 
   Size:       3521
 
BamHI G'GATC_C

Cuts at:    546     546 
   Size:       3521
 
BanI G'GyrC_C

Cuts at:    153     241     537     951    1199    1234     153 
   Size:         88     296     414     248      35    2440

  Fragments arranged by size: 

                2440     414     296     248      88      35
 
BanII G_rGCy'C

Cuts at:    370     535    1565     370 
   Size:        165    1030    2326

  Fragments arranged by size: 

                2326    1030     165
 
BbeI G_GCGC'C

Cuts at:    955    1203     955 
   Size:        248    3273
 
BbsI GAAGACnn'nnnn_

Cuts at:    431     431 
   Size:       3521
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:    112     146     270     304     112 
   Size:         34     124      34    3329

  Fragments arranged by size: 

                3329     124      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:    508    1419    2180    3521     508 
   Size:        911     761    1341     508

  Fragments arranged by size: 

                1341     911     761     508
 
BclI T'GATC_A

Cuts at:   1041    1041 
   Size:       3521
 
BglI GCCn_nnn'nGGC

Cuts at:    178     178 
   Size:       3521
 
BglII A'GATC_T

Cuts at:   1036    3073    1036 
   Size:       2037    1484
 
BlpI GC'TnA_GC

Cuts at:    633     633 
   Size:       3521
 
Bme1580I G_kGCm'C

Cuts at:   1146    1239    2295    1146 
   Size:         93    1056    2372

  Fragments arranged by size: 

                2372    1056      93
 
BmrI ACTGGGnnnn_n'

Cuts at:    733     814    1149    3293     733 
   Size:         81     335    2144     961

  Fragments arranged by size: 

                2144     961     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   1888    1888 
   Size:       3521
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    182     182 
   Size:       3521
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    697    1576    2068    2366     697 
   Size:        879     492     298    1852

  Fragments arranged by size: 

                1852     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    421    3225     421 
   Size:       2804     717
 
BsaAI yAC'GTr

Cuts at:    874     874 
   Size:       3521
 
BsaBI GATnn'nnATC

Cuts at:      6    1035    3072       6 
   Size:       1029    2037     455

  Fragments arranged by size: 

                2037    1029     455
 
BsaHI Gr'CG_yC

Cuts at:    952    1200    1902    3398     952 
   Size:        248     702    1496    1075

  Fragments arranged by size: 

                1496    1075     702     248
 
BsaWI w'CCGG_w

Cuts at:    710    1231    1909    2183    2330    2741     710 
   Size:        521     678     274     147     411    1490

  Fragments arranged by size: 

                1490     678     521     411     274     147
 
BseYI C'CCAG_C

Cuts at:    946    1704    2281    2777     946 
   Size:        758     577     496    1690

  Fragments arranged by size: 

                1690     758     577     496
 
BsiEI CG_ry'CG

Cuts at:    104    1063    1109    2317     104 
   Size:        959      46    1208    1308

  Fragments arranged by size: 

                1308    1208     959      46
 
BsiHKAI G_wGCw'C

Cuts at:    370     535    1313    1503    2295     370 
   Size:        165     778     190     792    1596

  Fragments arranged by size: 

                1596     792     778     190     165
 
Bsp1286I G_dGCh'C

Cuts at:    370     535    1146    1239    1313    1503    1565    2295     370 
   Size:        165     611      93      74     190      62     730    1596

  Fragments arranged by size: 

                1596     730     611     190     165      93      74      62
            
 
BspEI T'CCGG_A

Cuts at:   2741    2741 
   Size:       3521
 
BspHI T'CATG_A

Cuts at:   2697    2697 
   Size:       3521
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    571     785    1087    1468     571 
   Size:        214     302     381    2624

  Fragments arranged by size: 

                2624     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50    1813    1867    3351    3516      50 
   Size:       1763      54    1484     165      55

  Fragments arranged by size: 

                1763    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   1433    1433 
   Size:       3521
 
BsrFI r'CCGG_y

Cuts at:    142    1519    1700    1909     142 
   Size:       1377     181     209    1754

  Fragments arranged by size: 

                1754    1377     209     181
 
BssHII G'CGCG_C

Cuts at:    184     184 
   Size:       3521
 
BssSI C'ACGA_G

Cuts at:   1792    2150    1792 
   Size:        358    3163
 
BstAPI GCAn_nnn'nTGC

Cuts at:    898     898 
   Size:       3521
 
BstBI TT'CG_AA

Cuts at:   1882    1882 
   Size:       3521
 
BstYI r'GATC_y

Cuts at:      1     260     546    1013    1036    1371    1617    2629    2715 
   Size:        259     286     467      23     335     246    1012      86

Cuts at:   2715    2727    3073       1 
   Size:         12     346     449

  Fragments arranged by size: 

                1012     467     449     346     335     286     259     246
                  86      23      12
 
Bsu36I CC'TnA_GG

Cuts at:    473     473 
   Size:       3521
 
BtgI C'CryG_G

Cuts at:    107     157     245     501     107 
   Size:         50      88     256    3127

  Fragments arranged by size: 

                3127     256      88      50
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   1471    1652    1815    1471 
   Size:        181     163    3177

  Fragments arranged by size: 

                3177     181     163
 
BtsI GCAGTG_nn'

Cuts at:    764     764 
   Size:       3521
 
DraI TTT'AAA

Cuts at:    520     520 
   Size:       3521
 
DrdI GACnn_nn'nnGTC

Cuts at:   1227    2085    1227 
   Size:        858    2663
 
EaeI y'GGCC_r

Cuts at:    170    1106    1280    1671    1698    3043     170 
   Size:        936     174     391      27    1345     648

  Fragments arranged by size: 

                1345     936     648     391     174      27
 
EagI C'GGCC_G

Cuts at:   1106    1106 
   Size:       3521
 
EarI CTCTTCn'nnn_

Cuts at:   1544    1754    1544 
   Size:        210    3311
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    253    1729    2039    2185    3331     253 
   Size:       1476     310     146    1146     443

  Fragments arranged by size: 

                1476    1146     443     310     146
 
EcoICRI GAG'CTC

Cuts at:    368     533     368 
   Size:        165    3356
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    182    1346    1778    2525    2793     182 
   Size:       1164     432     747     268     910

  Fragments arranged by size: 

                1164     910     747     432     268
 
EcoO109I rG'GnC_Cy

Cuts at:    660     660 
   Size:       3521
 
EcoRI G'AATT_C

Cuts at:    525     525 
   Size:       3521
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   1540    1540 
   Size:       3521
 
FspI TGC'GCA

Cuts at:   1302    1302 
   Size:       3521
 
HaeII r_GCGC'y

Cuts at:    197     318     385     955    1203    2225    3204     197 
   Size:        121      67     570     248    1022     979     514

  Fragments arranged by size: 

                1022     979     570     514     248     121      67
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1363    1393    1395    1425    1363 
   Size:         30       2      30    3459

  Fragments arranged by size: 

                3459      30      30       2
 
HincII GTy'rAC

Cuts at:    281     560     281 
   Size:        279    3242
 
KasI G'GCGC_C

Cuts at:    951    1199     951 
   Size:        248    3273
 
KpnI G_GTAC'C

Cuts at:    541     541 
   Size:       3521
 
MluI A'CGCG_T

Cuts at:   2954    2954 
   Size:       3521
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   2192    2376    2192 
   Size:        184    3337
 
MscI TGG'CCA

Cuts at:    172    1282     172 
   Size:       1110    2411
 
MslI CAynn'nnrTG

Cuts at:    466    1510    1637     466 
   Size:       1044     127    2350

  Fragments arranged by size: 

                2350    1044     127
 
MspA1I CmG'CkG

Cuts at:    159     168     632     946    1306    2319    2564     159 
   Size:          9     464     314     360    1013     245    1116

  Fragments arranged by size: 

                1116    1013     464     360     314     245       9
 
NaeI GCC'GGC

Cuts at:   1702    1702 
   Size:       3521
 
NarI GG'CG_CC

Cuts at:    952    1200     952 
   Size:        248    3273
 
NcoI C'CATG_G

Cuts at:    107     107 
   Size:       3521
 
NgoMIV G'CCGG_C

Cuts at:   1700    1700 
   Size:       3521
 
NspI r_CATG'y

Cuts at:    574    1074    1981     574 
   Size:        500     907    2114

  Fragments arranged by size: 

                2114     907     500
 
PciI A'CATG_T

Cuts at:   1977    1977 
   Size:       3521
 
PflMI CCAn_nnn'nTGG

Cuts at:    205     205 
   Size:       3521
 
PmeI GTTT'AAAC

Cuts at:    520     520 
   Size:       3521
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2660    2692    2660 
   Size:         32    3489
 
PstI C_TGCA'G

Cuts at:    568    1253     568 
   Size:        685    2836
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       3521
 
PvuII CAG'CTG

Cuts at:    168     946    1306     168 
   Size:        778     360    2383

  Fragments arranged by size: 

                2383     778     360
 
RsrII CG'GwC_CG

Cuts at:   1716    1716 
   Size:       3521
 
SacI G_AGCT'C

Cuts at:    370     535     370 
   Size:        165    3356
 
SacII CC_GC'GG

Cuts at:    160     160 
   Size:       3521
 
SalI G'TCGA_C

Cuts at:    279     558     279 
   Size:        279    3242
 
SapI GCTCTTCn'nnn_

Cuts at:   1544    1754    1544 
   Size:        210    3311
 
SbfI CC_TGCA'GG

Cuts at:    568     568 
   Size:       3521
 
ScaI AGT'ACT

Cuts at:   3038    3038 
   Size:       3521
 
SfcI C'TryA_G

Cuts at:     33     231     564    1249    2242    2433      33 
   Size:        198     333     685     993     191    1121

  Fragments arranged by size: 

                1121     993     685     333     198     191
 
SfoI GGC'GCC

Cuts at:    953    1201     953 
   Size:        248    3273
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3521
 
SmaI CCC'GGG

Cuts at:    543     543 
   Size:       3521
 
SmlI C'TyrA_G

Cuts at:    676    1591    2083    2345     676 
   Size:        915     492     262    1852

  Fragments arranged by size: 

                1852     915     492     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       3521
 
SphI G_CATG'C

Cuts at:    574    1074     574 
   Size:        500    3021
 
StuI AGG'CCT

Cuts at:    403     403 
   Size:       3521
 
StyI C'CwwG_G

Cuts at:    107     176     655    1009     107 
   Size:         69     479     354    2619

  Fragments arranged by size: 

                2619     479     354      69
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   1104    1237    1696    1905    1104 
   Size:        133     459     209    2720

  Fragments arranged by size: 

                2720     459     209     133
 
TatI w'GTAC_w

Cuts at:   3036    3036 
   Size:       3521
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   3038    3070    3038 
   Size:         32    3489
 
Tth111I GACn'n_nGTC

Cuts at:   1318    1318 
   Size:       3521
 
XbaI T'CTAG_A

Cuts at:    552     552 
   Size:       3521
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   1016    1016 
   Size:       3521
 
XmaI C'CCGG_G

Cuts at:    541     541 
   Size:       3521
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI     AfeI   AflIII     AgeI    AlwNI
    ApaLI     ApoI     AseI    AsiSI     AvaI    BamHI     BanI    BanII
     BbeI     BbsI     BcgI    BciVI     BclI     BglI    BglII     BlpI
 Bme1580I     BmrI     BplI     BpmI    BpuEI     BsaI    BsaAI    BsaBI
    BsaHI    BsaWI    BseYI    BsiEI  BsiHKAI Bsp1286I    BspEI    BspHI
    BspMI    BsrBI    BsrDI    BsrFI   BssHII    BssSI   BstAPI    BstBI
    BstYI   Bsu36I     BtgI    BtgZI     BtsI     DraI     DrdI     EaeI
     EagI     EarI     EciI  EcoICRI  Eco57MI EcoO109I    EcoRI     FalI
     FspI    HaeII    Hin4I   HincII     KasI     KpnI     MluI     MmeI
     MscI     MslI   MspA1I     NaeI     NarI     NcoI   NgoMIV     NspI
     PciI    PflMI     PmeI     PpiI     PstI     PvuI    PvuII    RsrII
     SacI    SacII     SalI     SapI     SbfI     ScaI     SfcI     SfoI
     SgfI     SmaI     SmlI     SpeI     SphI     StuI     StyI    TaqII
     TatI     TstI  Tth111I     XbaI     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII    AflII     AhdI     AleI     AlfI     AloI     ApaI
     AscI    AvrII     BaeI    BbvCI    BfrBI    BmgBI     BmtI   Bpu10I
    BsaXI    BseRI     BsgI    BsiWI     BsmI    BsmBI    BsrGI   BstEII
    BstXI  BstZ17I     ClaI    CspCI   DraIII    EcoNI    EcoRV     FseI
    FspAI  HindIII     HpaI     MfeI     NdeI     NheI     NotI     NruI
     NsiI     PacI     PasI     PfoI     PmlI    PpuMI    PshAI     PsiI
   PspOMI    PspXI     PsrI    SanDI    SexAI     SfiI    SgrAI    SnaBI
     SrfI     SspI     SwaI     XhoI     XmnI     ZraI
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