Restriction map for pF1KB7188
##################################
# NO CUT ENZYMES (  117 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AflII       AflIII      AgeI        AhdI        
AlwI        AocI        Aor51HI     ApaI        ApoI        
AscI        AseI        AsnI        Asp718I     AspEI       
AspI        AviII       BamHI       BbrPI       BbvCI       
BciVI       BclI        BfrI        BlpI        Bpu1102I    
BsaBI       BsaI        BseAI       BsiWI       BsmI        
Bsp1407I    BspDI       BspEI       BspHI       BspLU11I    
BsrDI       BsrGI       Bst1107I    BstAPI      BstBI       
BstXI       BstZ17I     Bsu36I      CelII       ClaI        
DraI        DrdI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      Eco81I      EcoRI       EcoRV       
EcoT22I     FbaI        FseI        FspI        HincII      
HindII      HindIII     HpaI        KpnI        MamI        
MfeI        MluI        MroI        MunI        NcoI        
NdeI        NheI        NotI        NruI        NsiI        
NspV        PacI        PaeR7I      PflFI       PflMI       
PinAI       PmaCI       PmeI        PmlI        PshAI       
PshBI       Psp1406I    PstI        PvuI        RcaI        
SacI        SalI        SapI        SbfI        ScaI        
SexAI       SfiI        SfuI        SgrAI       SmlI        
SnaBI       SpeI        Sse8387I    SspBI       SspI        
StuI        SwaI        TfiI        Tth111I     Van91I      
XbaI        XhoI        


###################################
# ONE CUT ENZYMES (   29 enzymes) #
###################################

AlwNI        1253  CAGGTGCTG            CAG[ACGT][ACGT][ACGT]^CTG
Asp700I       747  GAAGGTCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvrII         165  CCTAGG               C^CTAGG
BglI          484  GCCCGCCTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII         194  AGATCT               A^GATCT
BlnI          165  CCTAGG               C^CTAGG
BsaWI         274  ACCGGA               [AT]^CCGG[AT]
BsiEI         459  CGACCG               CG[GA][CT]^CG
BspMI         809  ACCTGC               ACCTGC(4/8)
BssHII       1177  GCGCGC               G^CGCGC
BssSI        1083  CTCGTG               CACGAG(-5/-1)
BstEII        369  GGTGACC              G^GT[ACGT]ACC
BstPI         369  GGTGACC              G^GT[ACGT]ACC
BstYI         194  AGATCT               [GA]^GATC[CT]
DraIII        997  CACAGCGTG            CAC[ACGT][ACGT][ACGT]^GTG
EarI           93  GAAGAG               CTCTTC(1/4)
EcoO65I       369  GGTGACC              G^GT[ACGT]ACC
Ksp632I        93  GAAGAG               CTCTTC(1/4)
MflI          194  AGATCT               [GA]^GATC[CT]
MslI          330  CATCTCGGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NspI          476  GCATGC               [GA]CATG^[CT]
PpuMI         647  AGGACCT              [GA]G^G[AT]CC[CT]
SfaNI        1292  GCATC                GCATC(5/9)
SfcI          702  CTACAG               C^T[GA][CT]AG
SphI          476  GCATGC               GCATG^C
Tsp509I       235  AATT                 ^AATT
XcmI          608  CCACGGTGCACCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII         194  AGATCT               [GA]^GATC[CT]
XmnI          747  GAAGGTCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   23 enzymes) #
###################################

Alw44I        613  GTGCAC               G^TGCAC
              994  GTGCAC              
ApaLI         613  GTGCAC               G^TGCAC
              994  GTGCAC              
BbsI          159  GAAGAC               GAAGAC(2/6)
              751  GTCTTC              
BfaI           28  CTAG                 C^TAG
              166  CTAG                
BpuAI         159  GAAGAC               GAAGAC(2/6)
              751  GTCTTC              
BsaAI         757  TACGTG               [CT]AC^GT[GA]
              946  TACGTG              
BsmBI          36  CGTCTC               CGTCTC(1/5)
              766  GAGACG              
BstF5I       1293  CATCC                GGATG(2/0)
              261  GGATG               
CpoI           72  CGGACCG              CG^G[AT]CCG
             1184  CGGACCG             
DpnII         195  GATC                 ^GATC
              762  GATC                
EcoNI         618  CCTGGAGCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             1008  CCTAAGCGAGG         
EcoT14I       165  CCTAGG               C^C[AT][AT]GG
              530  CCAAGG              
FokI         1293  CATCC                GGATG(9/13)
              261  GGATG               
MaeI           28  CTAG                 C^TAG
              166  CTAG                
MboI          195  GATC                 ^GATC
              762  GATC                
MseI          218  TTAA                 T^TAA
              257  TTAA                
NdeII         195  GATC                 ^GATC
              762  GATC                
NlaIII        477  CATG                 CATG^
             1368  CATG                
PvuII        1110  CAGCTG               CAG^CTG
             1309  CAGCTG              
RsrII          72  CGGACCG              CG^G[AT]CCG
             1184  CGGACCG             
Sau3AI        195  GATC                 ^GATC
              762  GATC                
StyI          165  CCTAGG               C^C[AT][AT]GG
              530  CCAAGG              
Tru9I         218  TTAA                 T^TAA
              257  TTAA                


#########################################
# 3 OR MORE CUT ENZYMES (   89 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AluI        
AspHI       AvaI        AvaII       BalI        BanI        
BanII       BbeI        BbvI        BcgI        BcnI        
BmyI        BpmI        BsaHI       BsaJI       BseRI       
BsgI        BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       BsoBI       Bsp1286I    BsrBI       BsrFI       
BsrI        BssKI       BstNI       BstUI       Cac8I       
CfoI        Cfr10I      Cfr13I      DdeI        DraII       
DsaI        EaeI        EcoO109I    EcoRII      FauI        
Fnu4HI      HaeII       HaeIII      HapII       HgaI        
HhaI        Hin1I       HinP1I      HinfI       HpaII       
HphI        ItaI        KasI        KspI        MaeII       
MaeIII      MboII       MluNI       MnlI        MscI        
MspA1I      MspI        MvaI        MvnI        MwoI        
NaeI        NarI        NciI        NgoMIV      NlaIV       
PleI        RsaI        SacII       Sau96I      ScrFI       
SfoI        SmaI        TaqI        TseI        Tsp45I      
TspRI       TthHB8I     XmaCI       XmaI        
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