Restriction map for
pF1KB7188
(Circular) (Six-base) MAPSORT of: pF1KB7188.seq Check: 3706 from: 1 to: 4499
pF1KB7188 4499 bp
With 182 enzymes: SgfI *
June 20, 2008 10:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 926 926
Size: 4499
AccI GT'mk_AC
Cuts at: 1537 1537
Size: 4499
Acc65I G'GTAC_C
Cuts at: 1515 1515
Size: 4499
AclI AA'CG_TT
Cuts at: 4339 4339
Size: 4499
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2324 2756 3503 3771 2324
Size: 432 747 268 3052
Fragments arranged by size:
3052 747 432 268
AflIII A'CryG_T
Cuts at: 1851 2955 3932 1851
Size: 1104 977 2418
Fragments arranged by size:
2418 1104 977
AgeI A'CCGG_T
Cuts at: 2887 2887
Size: 4499
AlwNI CAG_nnn'CTG
Cuts at: 1366 1804 3371 1366
Size: 438 1567 2494
Fragments arranged by size:
2494 1567 438
ApaLI G'TGCA_C
Cuts at: 721 1102 3269 721
Size: 381 2167 1951
Fragments arranged by size:
2167 1951 381
ApoI r'AATT_y
Cuts at: 1503 3852 4108 1503
Size: 2349 256 1894
Fragments arranged by size:
2349 1894 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4499
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4499
AvaI C'yCGr_G
Cuts at: 165 705 766 961 972 978 1010 1038 1519
Size: 540 61 195 11 6 32 28 481
Cuts at: 1519 165
Size: 3145
Fragments arranged by size:
3145 540 481 195 61 32 28 11
6
AvrII C'CTAG_G
Cuts at: 273 273
Size: 4499
BamHI G'GATC_C
Cuts at: 1524 1524
Size: 4499
BanI G'GyrC_C
Cuts at: 209 277 739 847 930 1135 1162 1278 1435
Size: 68 462 108 83 205 27 116 157
Cuts at: 1435 1515 1929 2177 2212 209
Size: 80 414 248 35 2496
Fragments arranged by size:
2496 462 414 248 205 157 116 108
83 80 68 35 27
BanII G_rGCy'C
Cuts at: 712 766 962 979 1513 2543 712
Size: 54 196 17 534 1030 2668
Fragments arranged by size:
2668 1030 534 196 54 17
BbeI G_GCGC'C
Cuts at: 281 851 934 1139 1282 1933 2181 281
Size: 570 83 205 143 651 248 2599
Fragments arranged by size:
2599 651 570 248 205 143 83
BbsI GAAGACnn'nnnn_
Cuts at: 274 852 274
Size: 578 3921
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 111 124 145 177 211 656 690 90
Size: 21 13 21 32 34 445 34 3899
Fragments arranged by size:
3899 445 34 34 32 21 21 13
BciVI GTATCCnnnnn_n'
Cuts at: 2397 3158 4499 2397
Size: 761 1341 2397
Fragments arranged by size:
2397 1341 761
BclI T'GATC_A
Cuts at: 2019 2019
Size: 4499
BglI GCCn_nnn'nGGC
Cuts at: 598 598
Size: 4499
BglII A'GATC_T
Cuts at: 302 2014 4051 302
Size: 1712 2037 750
Fragments arranged by size:
2037 1712 750
BlpI GC'TnA_GC
Cuts at: 1611 1611
Size: 4499
Bme1580I G_kGCm'C
Cuts at: 725 1106 1165 2124 2217 3273 725
Size: 381 59 959 93 1056 1951
Fragments arranged by size:
1951 1056 959 381 93 59
BmgBI CAC'GTC
Cuts at: 557 557
Size: 4499
BmrI ACTGGGnnnn_n'
Cuts at: 630 1012 1711 1792 2127 4271 630
Size: 382 699 81 335 2144 858
Fragments arranged by size:
2144 858 699 382 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 987 2866 987
Size: 1879 2620
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 291 748 1343 291
Size: 457 595 3447
Fragments arranged by size:
3447 595 457
Bpu10I CC'TnA_GC
Cuts at: 760 1117 760
Size: 357 4142
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1675 2554 3046 3344 1675
Size: 879 492 298 2830
Fragments arranged by size:
2830 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4203 4203
Size: 4499
BsaAI yAC'GTr
Cuts at: 867 1056 1852 867
Size: 189 796 3514
Fragments arranged by size:
3514 796 189
BsaBI GATnn'nnATC
Cuts at: 6 2013 4050 6
Size: 2007 2037 455
Fragments arranged by size:
2037 2007 455
BsaHI Gr'CG_yC
Cuts at: 278 848 931 967 1136 1279 1930 2178 2880
Size: 570 83 36 169 143 651 248 702
Cuts at: 2880 4376 278
Size: 1496 401
Fragments arranged by size:
1496 702 651 570 401 248 169 143
83 36
BsaWI w'CCGG_w
Cuts at: 382 1688 2209 2887 3161 3308 3719 382
Size: 1306 521 678 274 147 411 1162
Fragments arranged by size:
1306 1162 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 138 219 529 1138 1225 138
Size: 81 310 609 87 3412
Fragments arranged by size:
3412 609 310 87 81
BseYI C'CCAG_C
Cuts at: 488 1220 1470 1924 2682 3259 3755 488
Size: 732 250 454 758 577 496 1232
Fragments arranged by size:
1232 758 732 577 496 454 250
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 508 1256 1447 508
Size: 748 191 3560
Fragments arranged by size:
3560 748 191
BsiEI CG_ry'CG
Cuts at: 104 570 2041 2087 3295 104
Size: 466 1471 46 1208 1308
Fragments arranged by size:
1471 1308 1208 466 46
BsiHKAI G_wGCw'C
Cuts at: 725 1106 1217 1513 2291 2481 3273 725
Size: 381 111 296 778 190 792 1951
Fragments arranged by size:
1951 792 778 381 296 190 111
BsmBI CGTCTCn'nnnn_
Cuts at: 150 868 150
Size: 718 3781
Bsp1286I G_dGCh'C
Cuts at: 712 725 766 962 979 1106 1165 1217 1513
Size: 13 41 196 17 127 59 52 296
Cuts at: 1513 2124 2217 2291 2481 2543 3273 712
Size: 611 93 74 190 62 730 1938
Fragments arranged by size:
1938 730 611 296 196 190 127 93
74 62 59 52 41 17 13
BspEI T'CCGG_A
Cuts at: 3719 3719
Size: 4499
BspHI T'CATG_A
Cuts at: 3675 3675
Size: 4499
BspMI ACCTGCnnnn'nnnn_
Cuts at: 926 1549 1763 2065 2446 926
Size: 623 214 302 381 2979
Fragments arranged by size:
2979 623 381 302 214
BsrBI CCG'CTC
Cuts at: 50 388 1149 1357 2791 2845 4329 4494 50
Size: 338 761 208 1434 54 1484 165 55
Fragments arranged by size:
1484 1434 761 338 208 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2411 2411
Size: 4499
BsrFI r'CCGG_y
Cuts at: 610 800 1082 2497 2678 2887 610
Size: 190 282 1415 181 209 2222
Fragments arranged by size:
2222 1415 282 209 190 181
BssHII G'CGCG_C
Cuts at: 1285 1285
Size: 4499
BssSI C'ACGA_G
Cuts at: 1191 2770 3128 1191
Size: 1579 358 2562
Fragments arranged by size:
2562 1579 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1876 1876
Size: 4499
BstBI TT'CG_AA
Cuts at: 2860 2860
Size: 4499
BstEII G'GTnAC_C
Cuts at: 477 477
Size: 4499
BstYI r'GATC_y
Cuts at: 1 302 1524 1991 2014 2349 2595 3607 3693
Size: 301 1222 467 23 335 246 1012 86
Cuts at: 3693 3705 4051 1
Size: 12 346 449
Fragments arranged by size:
1222 1012 467 449 346 335 301 246
86 23 12
BtgI C'CryG_G
Cuts at: 716 1130 1296 1323 1394 716
Size: 414 166 27 71 3821
Fragments arranged by size:
3821 414 166 71 27
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2449 2630 2793 2449
Size: 181 163 4155
Fragments arranged by size:
4155 181 163
BtsI GCAGTG_nn'
Cuts at: 548 651 1742 548
Size: 103 1091 3305
Fragments arranged by size:
3305 1091 103
DraI TTT'AAA
Cuts at: 1498 1498
Size: 4499
DraIII CAC_nnn'GTG
Cuts at: 1110 1110
Size: 4499
DrdI GACnn_nn'nnGTC
Cuts at: 2205 3063 2205
Size: 858 3641
EaeI y'GGCC_r
Cuts at: 260 434 457 501 683 713 947 1061 2084
Size: 174 23 44 182 30 234 114 1023
Cuts at: 2084 2258 2649 2676 4021 260
Size: 174 391 27 1345 738
Fragments arranged by size:
1345 1023 738 391 234 182 174 174
114 44 30 27 23
EagI C'GGCC_G
Cuts at: 2084 2084
Size: 4499
EarI CTCTTCn'nnn_
Cuts at: 196 2522 2732 196
Size: 2326 210 1963
Fragments arranged by size:
2326 1963 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 802 1237 2707 3017 3163 4309 802
Size: 435 1470 310 146 1146 992
Fragments arranged by size:
1470 1146 992 435 310 146
EcoICRI GAG'CTC
Cuts at: 1511 1511
Size: 4499
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 291 748 1343 2324 2756 3503 3771 291
Size: 457 595 981 432 747 268 1019
Fragments arranged by size:
1019 981 747 595 457 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 730 1120 730
Size: 390 4109
EcoO109I rG'GnC_Cy
Cuts at: 756 1006 1458 1638 756
Size: 250 452 180 3617
Fragments arranged by size:
3617 452 250 180
EcoRI G'AATT_C
Cuts at: 1503 1503
Size: 4499
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 876 2518 876
Size: 1642 2857
FspI TGC'GCA
Cuts at: 2280 2280
Size: 4499
HaeII r_GCGC'y
Cuts at: 281 851 897 934 1018 1139 1282 1933 2181
Size: 570 46 37 84 121 143 651 248
Cuts at: 2181 3203 4182 281
Size: 1022 979 598
Fragments arranged by size:
1022 979 651 598 570 248 143 121
84 46 37
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2341 2371 2373 2403 2341
Size: 30 2 30 4437
Fragments arranged by size:
4437 30 30 2
HincII GTy'rAC
Cuts at: 1538 1538
Size: 4499
KasI G'GCGC_C
Cuts at: 277 847 930 1135 1278 1929 2177 277
Size: 570 83 205 143 651 248 2599
Fragments arranged by size:
2599 651 570 248 205 143 83
KpnI G_GTAC'C
Cuts at: 1519 1519
Size: 4499
MluI A'CGCG_T
Cuts at: 3932 3932
Size: 4499
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3170 3354 3170
Size: 184 4315
MscI TGG'CCA
Cuts at: 436 459 503 2260 436
Size: 23 44 1757 2675
Fragments arranged by size:
2675 1757 44 23
MslI CAynn'nnrTG
Cuts at: 442 2488 2615 442
Size: 2046 127 2326
Fragments arranged by size:
2326 2046 127
MspA1I CmG'CkG
Cuts at: 284 1099 1132 1220 1298 1310 1325 1396 1419
Size: 815 33 88 78 12 15 71 23
Cuts at: 1419 1470 1610 1924 2284 3297 3542 284
Size: 51 140 314 360 1013 245 1241
Fragments arranged by size:
1241 1013 815 360 314 245 140 88
78 71 51 33 23 15 12
NaeI GCC'GGC
Cuts at: 612 802 1084 2680 612
Size: 190 282 1596 2431
Fragments arranged by size:
2431 1596 282 190
NarI GG'CG_CC
Cuts at: 278 848 931 1136 1279 1930 2178 278
Size: 570 83 205 143 651 248 2599
Fragments arranged by size:
2599 651 570 248 205 143 83
NgoMIV G'CCGG_C
Cuts at: 610 800 1082 2678 610
Size: 190 282 1596 2431
Fragments arranged by size:
2431 1596 282 190
NspI r_CATG'y
Cuts at: 588 1552 2052 2959 588
Size: 964 500 907 2128
Fragments arranged by size:
2128 964 907 500
PasI CC'CwG_GG
Cuts at: 1158 1158
Size: 4499
PciI A'CATG_T
Cuts at: 2955 2955
Size: 4499
PfoI T'CCnGG_A
Cuts at: 830 1445 830
Size: 615 3884
PmeI GTTT'AAAC
Cuts at: 1498 1498
Size: 4499
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3638 3670 3638
Size: 32 4467
PpuMI rG'GwC_Cy
Cuts at: 756 756
Size: 4499
PstI C_TGCA'G
Cuts at: 1546 2231 1546
Size: 685 3814
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4499
PvuII CAG'CTG
Cuts at: 1220 1419 1924 2284 1220
Size: 199 505 360 3435
Fragments arranged by size:
3435 505 360 199
RsrII CG'GwC_CG
Cuts at: 181 1293 2694 181
Size: 1112 1401 1986
Fragments arranged by size:
1986 1401 1112
SacI G_AGCT'C
Cuts at: 1513 1513
Size: 4499
SacII CC_GC'GG
Cuts at: 1133 1299 1326 1397 1133
Size: 166 27 71 4235
Fragments arranged by size:
4235 166 71 27
SalI G'TCGA_C
Cuts at: 1536 1536
Size: 4499
SapI GCTCTTCn'nnn_
Cuts at: 2522 2732 2522
Size: 210 4289
SbfI CC_TGCA'GG
Cuts at: 1546 1546
Size: 4499
ScaI AGT'ACT
Cuts at: 4016 4016
Size: 4499
SfcI C'TryA_G
Cuts at: 33 810 1542 2227 3220 3411 33
Size: 777 732 685 993 191 1121
Fragments arranged by size:
1121 993 777 732 685 191
SfoI GGC'GCC
Cuts at: 279 849 932 1137 1280 1931 2179 279
Size: 570 83 205 143 651 248 2599
Fragments arranged by size:
2599 651 570 248 205 143 83
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4499
SmaI CCC'GGG
Cuts at: 707 768 1040 1521 707
Size: 61 272 481 3685
Fragments arranged by size:
3685 481 272 61
SmlI C'TyrA_G
Cuts at: 1654 2569 3061 3323 1654
Size: 915 492 262 2830
Fragments arranged by size:
2830 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4499
SphI G_CATG'C
Cuts at: 588 1552 2052 588
Size: 964 500 3035
Fragments arranged by size:
3035 964 500
SrfI GCCC'GGGC
Cuts at: 1040 1040
Size: 4499
StyI C'CwwG_G
Cuts at: 273 638 1633 1987 273
Size: 365 995 354 2785
Fragments arranged by size:
2785 995 365 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 496 612 2082 2215 2674 2883 496
Size: 116 1470 133 459 209 2112
Fragments arranged by size:
2112 1470 459 209 133 116
TatI w'GTAC_w
Cuts at: 626 4014 626
Size: 3388 1111
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4016 4048 4016
Size: 32 4467
Tth111I GACn'n_nGTC
Cuts at: 2296 2296
Size: 4499
XbaI T'CTAG_A
Cuts at: 1530 1530
Size: 4499
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 114 723 1994 114
Size: 609 1271 2619
Fragments arranged by size:
2619 1271 609
XmaI C'CCGG_G
Cuts at: 705 766 1038 1519 705
Size: 61 272 481 3685
Fragments arranged by size:
3685 481 272 61
XmnI GAAnn'nnTTC
Cuts at: 859 859
Size: 4499
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI
BanII BbeI BbsI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI
BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NgoMIV NspI PasI
PciI PfoI PmeI PpiI PpuMI PstI PvuI PvuII
RsrII SacI SacII SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI SrfI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI ApaI
AscI BaeI BbvCI BfrBI BmtI BsaXI BsiWI BsmI
BsrGI BstXI BstZ17I Bsu36I ClaI CspCI EcoRV FseI
FspAI HindIII HpaI MfeI NcoI NdeI NheI NotI
NruI NsiI PacI PflMI PmlI PshAI PsiI PspOMI
PspXI PsrI SanDI SexAI SfiI SgrAI SnaBI SspI
StuI SwaI XhoI ZraI