Restriction map for pF1KB7188
 (Linear) (Six-base) MAPSORT of: KIBB7188.seq  Check: 8251  from: 1  to: 1386



KIBB7188.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 10:59  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     818    1386 
   Size:        818     568
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1258    1386 
   Size:       1258     128
 
ApaLI G'TGCA_C

Cuts at:      0     613     994    1386 
   Size:        613     381     392

  Fragments arranged by size: 

                 613     392     381
 
AvaI C'yCGr_G

Cuts at:      0      57     597     658     853     864     870     902     930 
   Size:         57     540      61     195      11       6      32      28

Cuts at:    930    1386 
   Size:        456

  Fragments arranged by size: 

                 540     456     195      61      57      32      28      11
                   6
 
AvrII C'CTAG_G

Cuts at:      0     165    1386 
   Size:        165    1221
 
BanI G'GyrC_C

Cuts at:      0     101     169     631     739     822    1027    1054    1170 
   Size:        101      68     462     108      83     205      27     116

Cuts at:   1170    1327    1386 
   Size:        157      59

  Fragments arranged by size: 

                 462     205     157     116     108     101      83      68
                  59      27
 
BanII G_rGCy'C

Cuts at:      0     604     658     854     871    1386 
   Size:        604      54     196      17     515

  Fragments arranged by size: 

                 604     515     196      54      17
 
BbeI G_GCGC'C

Cuts at:      0     173     743     826    1031    1174    1386 
   Size:        173     570      83     205     143     212

  Fragments arranged by size: 

                 570     212     205     173     143      83
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     166     744    1386 
   Size:        166     578     642

  Fragments arranged by size: 

                 642     578     166
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0       3      37      69     103     548     582    1386 
   Size:          3      34      32      34     445      34     804

  Fragments arranged by size: 

                 804     445      34      34      34      32       3
 
BglI GCCn_nnn'nGGC

Cuts at:      0     490    1386 
   Size:        490     896
 
BglII A'GATC_T

Cuts at:      0     194    1386 
   Size:        194    1192
 
Bme1580I G_kGCm'C

Cuts at:      0     617     998    1057    1386 
   Size:        617     381      59     329

  Fragments arranged by size: 

                 617     381     329      59
 
BmgBI CAC'GTC

Cuts at:      0     449    1386 
   Size:        449     937
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     522     904    1386 
   Size:        522     382     482

  Fragments arranged by size: 

                 522     482     382
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     879    1386 
   Size:        879     507
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     183     640    1235    1386 
   Size:        183     457     595     151

  Fragments arranged by size: 

                 595     457     183     151
 
Bpu10I CC'TnA_GC

Cuts at:      0     652    1009    1386 
   Size:        652     357     377

  Fragments arranged by size: 

                 652     377     357
 
BsaAI yAC'GTr

Cuts at:      0     759     948    1386 
   Size:        759     189     438

  Fragments arranged by size: 

                 759     438     189
 
BsaHI Gr'CG_yC

Cuts at:      0     170     740     823     859    1028    1171    1386 
   Size:        170     570      83      36     169     143     215

  Fragments arranged by size: 

                 570     215     170     169     143      83      36
 
BsaWI w'CCGG_w

Cuts at:      0     274    1386 
   Size:        274    1112
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      30     111     421    1030    1117    1386 
   Size:         30      81     310     609      87     269

  Fragments arranged by size: 

                 609     310     269      87      81      30
 
BseYI C'CCAG_C

Cuts at:      0     380    1112    1362    1386 
   Size:        380     732     250      24

  Fragments arranged by size: 

                 732     380     250      24
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     400    1148    1339    1386 
   Size:        400     748     191      47

  Fragments arranged by size: 

                 748     400     191      47
 
BsiEI CG_ry'CG

Cuts at:      0     462    1386 
   Size:        462     924
 
BsiHKAI G_wGCw'C

Cuts at:      0     617     998    1109    1386 
   Size:        617     381     111     277

  Fragments arranged by size: 

                 617     381     277     111
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0      42     760    1386 
   Size:         42     718     626

  Fragments arranged by size: 

                 718     626      42
 
Bsp1286I G_dGCh'C

Cuts at:      0     604     617     658     854     871     998    1057    1109 
   Size:        604      13      41     196      17     127      59      52

Cuts at:   1109    1386 
   Size:        277

  Fragments arranged by size: 

                 604     277     196     127      59      52      41      17
                  13
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     818    1386 
   Size:        818     568
 
BsrBI CCG'CTC

Cuts at:      0     280    1041    1249    1386 
   Size:        280     761     208     137

  Fragments arranged by size: 

                 761     280     208     137
 
BsrFI r'CCGG_y

Cuts at:      0     502     692     974    1386 
   Size:        502     190     282     412

  Fragments arranged by size: 

                 502     412     282     190
 
BssHII G'CGCG_C

Cuts at:      0    1177    1386 
   Size:       1177     209
 
BssSI C'ACGA_G

Cuts at:      0    1083    1386 
   Size:       1083     303
 
BstEII G'GTnAC_C

Cuts at:      0     369    1386 
   Size:        369    1017
 
BstYI r'GATC_y

Cuts at:      0     194    1386 
   Size:        194    1192
 
BtgI C'CryG_G

Cuts at:      0     608    1022    1188    1215    1286    1386 
   Size:        608     414     166      27      71     100

  Fragments arranged by size: 

                 608     414     166     100      71      27
 
BtsI GCAGTG_nn'

Cuts at:      0     440     543    1386 
   Size:        440     103     843

  Fragments arranged by size: 

                 843     440     103
 
DraIII CAC_nnn'GTG

Cuts at:      0    1002    1386 
   Size:       1002     384
 
EaeI y'GGCC_r

Cuts at:      0     152     326     349     393     575     605     839     953 
   Size:        152     174      23      44     182      30     234     114

Cuts at:    953    1386 
   Size:        433

  Fragments arranged by size: 

                 433     234     182     174     152     114      44      30
                  23
 
EarI CTCTTCn'nnn_

Cuts at:      0      88    1386 
   Size:         88    1298
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     694    1129    1386 
   Size:        694     435     257

  Fragments arranged by size: 

                 694     435     257
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     183     640    1235    1386 
   Size:        183     457     595     151

  Fragments arranged by size: 

                 595     457     183     151
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     622    1012    1386 
   Size:        622     390     374

  Fragments arranged by size: 

                 622     390     374
 
EcoO109I rG'GnC_Cy

Cuts at:      0     648     898    1350    1386 
   Size:        648     250     452      36

  Fragments arranged by size: 

                 648     452     250      36
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     768    1386 
   Size:        768     618
 
HaeII r_GCGC'y

Cuts at:      0     173     743     789     826     910    1031    1174    1386 
   Size:        173     570      46      37      84     121     143     212

  Fragments arranged by size: 

                 570     212     173     143     121      84      46      37
            
 
KasI G'GCGC_C

Cuts at:      0     169     739     822    1027    1170    1386 
   Size:        169     570      83     205     143     216

  Fragments arranged by size: 

                 570     216     205     169     143      83
 
MscI TGG'CCA

Cuts at:      0     328     351     395    1386 
   Size:        328      23      44     991

  Fragments arranged by size: 

                 991     328      44      23
 
MslI CAynn'nnrTG

Cuts at:      0     334    1386 
   Size:        334    1052
 
MspA1I CmG'CkG

Cuts at:      0     176     991    1024    1112    1190    1202    1217    1288 
   Size:        176     815      33      88      78      12      15      71

Cuts at:   1288    1311    1362    1386 
   Size:         23      51      24

  Fragments arranged by size: 

                 815     176      88      78      71      51      33      24
                  23      15      12
 
NaeI GCC'GGC

Cuts at:      0     504     694     976    1386 
   Size:        504     190     282     410

  Fragments arranged by size: 

                 504     410     282     190
 
NarI GG'CG_CC

Cuts at:      0     170     740     823    1028    1171    1386 
   Size:        170     570      83     205     143     215

  Fragments arranged by size: 

                 570     215     205     170     143      83
 
NgoMIV G'CCGG_C

Cuts at:      0     502     692     974    1386 
   Size:        502     190     282     412

  Fragments arranged by size: 

                 502     412     282     190
 
NspI r_CATG'y

Cuts at:      0     480    1386 
   Size:        480     906
 
PasI CC'CwG_GG

Cuts at:      0    1050    1386 
   Size:       1050     336
 
PfoI T'CCnGG_A

Cuts at:      0     722    1337    1386 
   Size:        722     615      49

  Fragments arranged by size: 

                 722     615      49
 
PpuMI rG'GwC_Cy

Cuts at:      0     648    1386 
   Size:        648     738
 
PvuII CAG'CTG

Cuts at:      0    1112    1311    1386 
   Size:       1112     199      75

  Fragments arranged by size: 

                1112     199      75
 
RsrII CG'GwC_CG

Cuts at:      0      73    1185    1386 
   Size:         73    1112     201

  Fragments arranged by size: 

                1112     201      73
 
SacII CC_GC'GG

Cuts at:      0    1025    1191    1218    1289    1386 
   Size:       1025     166      27      71      97

  Fragments arranged by size: 

                1025     166      97      71      27
 
SfcI C'TryA_G

Cuts at:      0     702    1386 
   Size:        702     684
 
SfoI GGC'GCC

Cuts at:      0     171     741     824    1029    1172    1386 
   Size:        171     570      83     205     143     214

  Fragments arranged by size: 

                 570     214     205     171     143      83
 
SmaI CCC'GGG

Cuts at:      0     599     660     932    1386 
   Size:        599      61     272     454

  Fragments arranged by size: 

                 599     454     272      61
 
SphI G_CATG'C

Cuts at:      0     480    1386 
   Size:        480     906
 
SrfI GCCC'GGGC

Cuts at:      0     932    1386 
   Size:        932     454
 
StyI C'CwwG_G

Cuts at:      0     165     530    1386 
   Size:        165     365     856

  Fragments arranged by size: 

                 856     365     165
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     388     504    1386 
   Size:        388     116     882

  Fragments arranged by size: 

                 882     388     116
 
TatI w'GTAC_w

Cuts at:      0     518    1386 
   Size:        518     868
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     615    1386 
   Size:        615     771
 
XmaI C'CCGG_G

Cuts at:      0     597     658     930    1386 
   Size:        597      61     272     456

  Fragments arranged by size: 

                 597     456     272      61
 
XmnI GAAnn'nnTTC

Cuts at:      0     751    1386 
   Size:        751     635
 

 Enzymes that do cut:

     AarI    AlwNI    ApaLI     AvaI    AvrII     BanI    BanII     BbeI
     BbsI     BcgI     BglI    BglII Bme1580I    BmgBI     BmrI     BplI
     BpmI   Bpu10I    BsaAI    BsaHI    BsaWI    BseRI    BseYI     BsgI
    BsiEI  BsiHKAI    BsmBI Bsp1286I    BspMI    BsrBI    BsrFI   BssHII
    BssSI   BstEII    BstYI     BtgI     BtsI   DraIII     EaeI     EarI
     EciI  Eco57MI    EcoNI EcoO109I     FalI    HaeII     KasI     MscI
     MslI   MspA1I     NaeI     NarI   NgoMIV     NspI     PasI     PfoI
    PpuMI    PvuII    RsrII    SacII     SfcI     SfoI     SmaI     SphI
     SrfI     StyI    TaqII     TatI     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI     ApaI     ApoI     AscI
     AseI    AsiSI     BaeI    BamHI    BbvCI    BciVI     BclI    BfrBI
     BlpI     BmtI    BpuEI     BsaI    BsaBI    BsaXI    BsiWI     BsmI
    BspEI    BspHI    BsrDI    BsrGI   BstAPI    BstBI    BstXI  BstZ17I
   Bsu36I    BtgZI     ClaI    CspCI     DraI     DrdI     EagI  EcoICRI
    EcoRI    EcoRV     FseI     FspI    FspAI    Hin4I   HincII  HindIII
     HpaI     KpnI     MfeI     MluI     MmeI     NcoI     NdeI     NheI
     NotI     NruI     NsiI     PacI     PciI    PflMI     PmeI     PmlI
     PpiI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI
     SacI     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI
     SgfI    SgrAI     SmlI    SnaBI     SpeI     SspI     StuI     SwaI
     TstI  Tth111I     XbaI     XhoI     ZraI
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