Restriction map for pF1KB7186
##################################
# NO CUT ENZYMES (  119 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcyI        AflII       AgeI        AhdI        Aor51HI     
AscI        AseI        AsnI        Asp718I     AspEI       
AvaII       AviII       AvrII       BamHI       BanI        
BbeI        BbvCI       BcgI        BciVI       BclI        
BfaI        BfrI        BglI        BglII       BlnI        
BsaBI       BsaHI       BseAI       BsiWI       BsmFI       
BspDI       BspEI       BspHI       BspLU11I    BsrDI       
BsrFI       BssHII      Bst1107I    BstBI       BstEII      
BstPI       BstXI       BstZ17I     Cfr10I      ClaI        
CpoI        DraI        Eam1105I    Eco47III    EcoNI       
EcoO65I     EcoRI       EcoRV       EcoT22I     FbaI        
FseI        FspI        HgaI        Hin1I       HincII      
HindII      HindIII     HpaI        KasI        KpnI        
MaeI        MaeIII      MamI        MfeI        MluI        
MroI        MseI        MunI        NaeI        NarI        
NcoI        NdeI        NgoMIV      NheI        NotI        
NruI        NsiI        NspV        PacI        PaeR7I      
PflMI       PinAI       PmeI        PpuMI       PshAI       
PshBI       Psp1406I    PvuI        RcaI        RsrII       
SalI        SbfI        ScaI        SfiI        SfoI        
SfuI        SgrAI       SnaBI       SpeI        SphI        
Sse8387I    SspI        StuI        SwaI        Tru9I       
Tsp45I      Van91I      XbaI        XhoI        


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# ONE CUT ENZYMES (   38 enzymes) #
###################################

AcsI         1117  AAATTC               [GA]^AATT[CT]
AflIII        914  ACGTGT               A^C[GA][CT]GT
Alw44I       1295  GTGCAC               G^TGCAC
AocI          586  CCTAAGG              CC^T[ACGT]AGG
ApaLI        1295  GTGCAC               G^TGCAC
ApoI         1117  AAATTC               [GA]^AATT[CT]
Asp700I       858  GAATCGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI          309  GACTGGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BalI          722  TGGCCA               TGG^CCA
BbsI          813  GAAGAC               GAAGAC(2/6)
BpuAI         813  GAAGAC               GAAGAC(2/6)
BsgI          985  GTGCAG               GTGCAG(16/14)
BsiEI         886  CGGCCG               CG[GA][CT]^CG
BsmBI         452  GAGACG               CGTCTC(1/5)
BsmI          538  GCATTC               GAATGC(1/-1)
Bsp1407I      470  TGTACA               T^GTACA
BsrBI         337  CCGCTC               CCGCTC(-3/-3)
BsrGI         470  TGTACA               T^GTACA
Bsu36I        586  CCTAAGG              CC^T[ACGT]AGG
DraIII       1261  CACGGTGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI          454  GACGTGCTGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI          886  CGGCCG               C^GGCCG
EclXI         886  CGGCCG               C^GGCCG
Eco52I        886  CGGCCG               C^GGCCG
Eco81I        586  CCTAAGG              CC^T[ACGT]AGG
KspI          385  CCGCGG               CCGC^GG
MluNI         722  TGGCCA               TGG^CCA
MscI          722  TGGCCA               TGG^CCA
MslI          504  CACGGCTGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NspI          467  GCATGT               [GA]CATG^[CT]
PflFI         309  GACTGGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
SacI          783  GAGCTC               GAGCT^C
SacII         385  CCGCGG               CCGC^GG
SexAI         560  ACCTGGT              A^CC[AT]GGT
SmlI          183  CTTGAG               C^T[CT][GA]AG
SspBI         470  TGTACA               T^GTACA
Tth111I       309  GACTGGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XmnI          858  GAATCGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   23 enzymes) #
###################################

ApaI          159  GGGCCC               GGGCC^C
             1303  GGGCCC              
BbrPI         913  CACGTG               CAC^GTG
             1236  CACGTG              
BsaAI         913  CACGTG               [CT]AC^GT[GA]
             1236  CACGTG              
BsaI          314  GGTCTC               GGTCTC(1/5)
              564  GGTCTC              
BsaWI         110  TCCGGT               [AT]^CCGG[AT]
             1036  ACCGGA              
BseRI         331  GAGGAG               GAGGAG(10/8)
             1040  GAGGAG              
BspMI         806  ACCTGC               ACCTGC(4/8)
             1160  ACCTGC              
BssSI         616  CACGAG               CACGAG(-5/-1)
              730  CTCGTG              
BstAPI        369  GCAGGAGATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
              639  GCAGCAGCTGC         
BstYI         360  GGATCT               [GA]^GATC[CT]
             1199  AGATCC              
EaeI          722  TGGCCA               [CT]^GGCC[GA]
              886  CGGCCG              
EarI          415  CTCTTC               CTCTTC(1/4)
              149  GAAGAG              
HaeII         240  AGCGCC               [GA]GCGC^[CT]
             1051  AGCGCC              
Ksp632I       415  CTCTTC               CTCTTC(1/4)
              149  GAAGAG              
MflI          360  GGATCT               [GA]^GATC[CT]
             1199  AGATCC              
NlaIII        212  CATG                 CATG^
              468  CATG                
PleI          600  GACTC                GAGTC(4/5)
             1250  GAGTC               
PmaCI         913  CACGTG               CAC^GTG
             1236  CACGTG              
PmlI          913  CACGTG               CAC^GTG
             1236  CACGTG              
SapI          149  GAAGAGC              GCTCTTC(1/4)
              414  GCTCTTC             
Tsp509I       928  AATT                 ^AATT
             1118  AATT                
XcmI          893  CCACGGTTATCCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
              912  CCACGTGTTCCCTGG     
XhoII         360  GGATCT               [GA]^GATC[CT]
             1199  AGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   78 enzymes) #
#########################################

AccII       AciI        AfaI        AluI        AlwI        
AlwNI       AspHI       AvaI        BanII       BbvI        
BcnI        BlpI        BmyI        BpmI        Bpu1102I    
BsaJI       BsiHKAI     BsiYI       BslI        BsmAI       
BsoBI       Bsp1286I    BsrI        BssKI       BstF5I      
BstNI       BstUI       Cac8I       CelII       CfoI        
Cfr13I      DdeI        DpnII       DraII       DsaI        
EcoO109I    EcoRII      EcoT14I     FauI        Fnu4HI      
FokI        HaeIII      HapII       HhaI        HinP1I      
HinfI       HpaII       HphI        ItaI        MaeII       
MboI        MboII       MnlI        MspA1I      MspI        
MvaI        MvnI        MwoI        NciI        NdeII       
NlaIV       PstI        PvuII       RsaI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        SmaI        
StyI        TaqI        TfiI        TseI        TspRI       
TthHB8I     XmaCI       XmaI        
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