Restriction map for pF1KB7185
 (Linear) (Six-base) MAPSORT of: KIBB7185.seq  Check: 8815  from: 1  to: 1332



KIBB7185.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 10:59  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     325    1332 
   Size:        325    1007
 
AccI GT'mk_AC

Cuts at:      0     756    1332 
   Size:        756     576
 
Acc65I G'GTAC_C

Cuts at:      0     122    1332 
   Size:        122    1210
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1028    1332 
   Size:       1028     304
 
AfeI AGC'GCT

Cuts at:      0     637     861    1332 
   Size:        637     224     471

  Fragments arranged by size: 

                 637     471     224
 
AleI CACnn'nnGTG

Cuts at:      0     705    1332 
   Size:        705     627
 
ApaI G_GGCC'C

Cuts at:      0     507    1332 
   Size:        507     825
 
ApoI r'AATT_y

Cuts at:      0     112     650    1108    1120    1332 
   Size:        112     538     458      12     212

  Fragments arranged by size: 

                 538     458     212     112      12
 
AvaI C'yCGr_G

Cuts at:      0     824    1332 
   Size:        824     508
 
BanI G'GyrC_C

Cuts at:      0       7     122     699    1332 
   Size:          7     115     577     633

  Fragments arranged by size: 

                 633     577     115       7
 
BanII G_rGCy'C

Cuts at:      0      80     507    1332 
   Size:         80     427     825

  Fragments arranged by size: 

                 825     427      80
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1016    1332 
   Size:       1016     316
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     721     755    1332 
   Size:        721      34     577

  Fragments arranged by size: 

                 721     577      34
 
BglI GCCn_nnn'nGGC

Cuts at:      0     828    1332 
   Size:        828     504
 
BlpI GC'TnA_GC

Cuts at:      0     597    1332 
   Size:        597     735
 
Bme1580I G_kGCm'C

Cuts at:      0     507    1332 
   Size:        507     825
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      56    1332 
   Size:         56    1276
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     346    1332 
   Size:        346     986
 
BseYI C'CCAG_C

Cuts at:      0     638    1296    1332 
   Size:        638     658      36

  Fragments arranged by size: 

                 658     638      36
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     127     597    1332 
   Size:        127     470     735

  Fragments arranged by size: 

                 735     470     127
 
BsiHKAI G_wGCw'C

Cuts at:      0     305     493    1277    1332 
   Size:        305     188     784      55

  Fragments arranged by size: 

                 784     305     188      55
 
Bsp1286I G_dGCh'C

Cuts at:      0      80     305     493     507    1277    1332 
   Size:         80     225     188      14     770      55

  Fragments arranged by size: 

                 770     225     188      80      55      14
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     149     325    1332 
   Size:        149     176    1007

  Fragments arranged by size: 

                1007     176     149
 
BssSI C'ACGA_G

Cuts at:      0     304    1332 
   Size:        304    1028
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1272    1332 
   Size:       1272      60
 
BstEII G'GTnAC_C

Cuts at:      0     261    1332 
   Size:        261    1071
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     570    1332 
   Size:        570     762
 
BstYI r'GATC_y

Cuts at:      0     761     884    1332 
   Size:        761     123     448

  Fragments arranged by size: 

                 761     448     123
 
Bsu36I CC'TnA_GG

Cuts at:      0     557    1332 
   Size:        557     775
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1147    1332 
   Size:       1147     185
 
BtsI GCAGTG_nn'

Cuts at:      0     239    1332 
   Size:        239    1093
 
DraI TTT'AAA

Cuts at:      0      86    1125    1332 
   Size:         86    1039     207

  Fragments arranged by size: 

                1039     207      86
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1028    1332 
   Size:       1028     304
 
EcoO109I rG'GnC_Cy

Cuts at:      0     165    1332 
   Size:        165    1167
 
EcoRI G'AATT_C

Cuts at:      0     112    1332 
   Size:        112    1220
 
EcoRV GAT'ATC

Cuts at:      0     345    1332 
   Size:        345     987
 
HaeII r_GCGC'y

Cuts at:      0     639     863    1332 
   Size:        639     224     469

  Fragments arranged by size: 

                 639     469     224
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     378     410    1234    1266    1332 
   Size:        378      32     824      32      66

  Fragments arranged by size: 

                 824     378      66      32      32
 
HincII GTy'rAC

Cuts at:      0     757    1332 
   Size:        757     575
 
KpnI G_GTAC'C

Cuts at:      0     126    1332 
   Size:        126    1206
 
MslI CAynn'nnrTG

Cuts at:      0     187     547     568     705    1332 
   Size:        187     360      21     137     627

  Fragments arranged by size: 

                 627     360     187     137      21
 
PfoI T'CCnGG_A

Cuts at:      0     850    1332 
   Size:        850     482
 
PpuMI rG'GwC_Cy

Cuts at:      0     165    1332 
   Size:        165    1167
 
PsiI TTA'TAA

Cuts at:      0     547    1091    1332 
   Size:        547     544     241

  Fragments arranged by size: 

                 547     544     241
 
PspOMI G'GGCC_C

Cuts at:      0     503    1332 
   Size:        503     829
 
PspXI vC'TCGA_Gb

Cuts at:      0     824    1332 
   Size:        824     508
 
PstI C_TGCA'G

Cuts at:      0     809    1332 
   Size:        809     523
 
SalI G'TCGA_C

Cuts at:      0     755    1332 
   Size:        755     577
 
SexAI A'CCwGG_T

Cuts at:      0     355    1332 
   Size:        355     977
 
SfcI C'TryA_G

Cuts at:      0     397     779     805    1332 
   Size:        397     382      26     527

  Fragments arranged by size: 

                 527     397     382      26
 
SmlI C'TyrA_G

Cuts at:      0      71     824    1332 
   Size:         71     753     508

  Fragments arranged by size: 

                 753     508      71
 
SspI AAT'ATT

Cuts at:      0     737    1332 
   Size:        737     595
 
StyI C'CwwG_G

Cuts at:      0     446    1149    1332 
   Size:        446     703     183

  Fragments arranged by size: 

                 703     446     183
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     306     338    1332 
   Size:        306      32     994

  Fragments arranged by size: 

                 994     306      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     405    1332 
   Size:        405     927
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     336    1325    1332 
   Size:        336     989       7

  Fragments arranged by size: 

                 989     336       7
 
XhoI C'TCGA_G

Cuts at:      0     824    1332 
   Size:        824     508
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AcuI     AfeI     AleI     ApaI     ApoI
     AvaI     BanI    BanII     BbsI     BcgI     BglI     BlpI Bme1580I
    BpuEI     BsaI    BseYI     BsgI  BsiHKAI Bsp1286I    BspMI    BssSI
   BstAPI   BstEII    BstXI    BstYI   Bsu36I    BtgZI     BtsI     DraI
  Eco57MI EcoO109I    EcoRI    EcoRV    HaeII    Hin4I   HincII     KpnI
     MslI     PfoI    PpuMI     PsiI   PspOMI    PspXI     PstI     SalI
    SexAI     SfcI     SmlI     SspI     StyI     TstI  Tth111I     XcmI
     XhoI

 Enzymes that do not cut: 

    AatII     AclI    AflII   AflIII     AgeI     AhdI     AlfI     AloI
    AlwNI    ApaLI     AscI     AseI    AsiSI    AvrII     BaeI    BamHI
     BbeI    BbvCI    BciVI     BclI    BfrBI    BglII    BmgBI     BmrI
     BmtI     BplI     BpmI   Bpu10I    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BseRI    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspHI
    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BstBI  BstZ17I     BtgI
     ClaI    CspCI   DraIII     DrdI     EaeI     EagI     EarI     EciI
  EcoICRI    EcoNI     FalI     FseI     FspI    FspAI  HindIII     HpaI
     KasI     MfeI     MluI     MmeI     MscI   MspA1I     NaeI     NarI
     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI
     PacI     PasI     PciI    PflMI     PmeI     PmlI     PpiI    PshAI
     PsrI     PvuI    PvuII    RsrII     SacI    SacII    SanDI     SapI
     SbfI     ScaI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI
     SpeI     SphI     SrfI     StuI     SwaI    TaqII     TatI     XbaI
     XmaI     XmnI     ZraI
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