Restriction map for pF1KB7176
##################################
# NO CUT ENZYMES (  130 enzymes) #
##################################

AatII       AccIII      AclI        AcsI        AflII       
AflIII      AgeI        AhdI        Alw44I      AocI        
Aor51HI     ApaLI       ApoI        AscI        AseI        
AsnI        Asp700I     AspEI       AspHI       AspI        
AviII       BamHI       BbeI        BbrPI       BcgI        
BciVI       BclI        BfrI        BglII       BlpI        
BpmI        Bpu1102I    BsaBI       BsaI        BsaWI       
BseAI       BsiHKAI     BsiWI       BsmBI       BsmI        
Bsp1407I    BspDI       BspEI       BspHI       BspLU11I    
BsrDI       BsrGI       BssHII      Bst1107I    BstAPI      
BstBI       BstEII      BstPI       BstYI       BstZ17I     
Bsu36I      CelII       ClaI        CpoI        DraI        
DsaI        Eam1105I    Eco47III    Eco81I      EcoO65I     
EcoRI       EcoRV       EcoT22I     FbaI        FspI        
HindIII     HpaI        KasI        KspI        MaeIII      
MamI        MfeI        MflI        MluI        MroI        
MseI        MunI        NarI        NcoI        NdeI        
NotI        NruI        NsiI        NspI        NspV        
PacI        PaeR7I      PflFI       PflMI       PinAI       
PmaCI       PmeI        PmlI        PshAI       PshBI       
Psp1406I    PvuI        RcaI        RsrII       SacI        
SacII       SapI        ScaI        SexAI       SfoI        
SfuI        SgrAI       SmaI        SmlI        SnaBI       
SpeI        SphI        SspBI       SspI        SwaI        
Tru9I       Tsp45I      Tsp509I     Tth111I     Van91I      
XhoI        XhoII       XmaCI       XmaI        XmnI        


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# ONE CUT ENZYMES (   44 enzymes) #
###################################

Acc65I         80  GGTACC               G^GTACC
AccI          328  GTCGAC               GT^[AC][GT]AC
AccII         407  CGCG                 CG^CG
AcyI          982  GACGCC               G[GA]^CG[CT]C
AfaI           81  GTAC                 GT^AC
AlwI          342  GATCC                GGATC(4/5)
Asp718I        80  GGTACC               G^GTACC
AvrII         739  CCTAGG               C^CTAGG
BalI          116  TGGCCA               TGG^CCA
BbsI          214  GAAGAC               GAAGAC(2/6)
BbvCI         349  GCTGAGG              CCTCAGC(-5/-2)
BlnI          739  CCTAGG               C^CTAGG
BpuAI         214  GAAGAC               GAAGAC(2/6)
BsaAI         439  TACGTG               [CT]AC^GT[GA]
BsaHI         982  GACGCC               G[GA]^CG[CT]C
BsmAI         691  GAGAC                GTCTC(1/5)
BspMI          83  ACCTGC               ACCTGC(4/8)
BsrBI         143  CCGCTC               CCGCTC(-3/-3)
BssSI         675  CACGAG               CACGAG(-5/-1)
BstF5I        177  CATCC                GGATG(2/0)
BstUI         407  CGCG                 CG^CG
DraIII        485  CACCCAGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI         1024  GACGTTTGGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FokI          177  CATCC                GGATG(9/13)
FseI          451  GGCCGGCC             GGCCGG^CC
HgaI          982  GACGC                GACGC(5/10)
Hin1I         982  GACGCC               G[GA]^CG[CT]C
HphI          132  GGTGA                GGTGA(8/7)
KpnI           80  GGTACC               GGTAC^C
MluNI         116  TGGCCA               TGG^CCA
MscI          116  TGGCCA               TGG^CCA
MslI          675  CACGAGAGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI          407  CGCG                 CG^CG
NheI          552  GCTAGC               G^CTAGC
RsaI           81  GTAC                 GT^AC
SalI          328  GTCGAC               G^TCGAC
SbfI          577  CCTGCAGG             CCTGCA^GG
Sse8387I      577  CCTGCAGG             CCTGCA^GG
StuI          640  AGGCCT               AGG^CCT
TaqI          329  TCGA                 T^CGA
TfiI          135  GATTC                G^A[AT]TC
TthHB8I       329  TCGA                 T^CGA
XbaI          978  TCTAGA               T^CTAGA
XcmI          119  CCATGATCGCCTTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   20 enzymes) #
###################################

AlwNI         572  CAGTTCCTG            CAG[ACGT][ACGT][ACGT]^CTG
              776  CAGTTCCTG           
AvaI          950  CCCGAG               C^[CT]CG[GA]G
             1043  CTCGGG              
BcnI          527  CCGGG                CC^[GC]GG
              547  CCGGG               
BseRI          66  GAGGAG               GAGGAG(10/8)
              601  GAGGAG              
BsoBI         950  CCCGAG               C^[CT]CG[GA]G
             1043  CTCGGG              
BstXI         422  CCAAGGACCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              674  CCACGAGAGTGG        
EagI          450  CGGCCG               C^GGCCG
              463  CGGCCG              
EclXI         450  CGGCCG               C^GGCCG
              463  CGGCCG              
Eco52I        450  CGGCCG               C^GGCCG
              463  CGGCCG              
EcoNI         203  CCTTCTTCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             1060  CCTGGCCCAGG         
HaeII         354  GGCGCT               [GA]GCGC^[CT]
              386  GGCGCT              
HincII        328  GTCGAC               GT[CT]^[GA]AC
              912  GTCAAC              
HindII        328  GTCGAC               GT[CT]^[GA]AC
              912  GTCAAC              
NaeI          383  GCCGGC               GCC^GGC
              452  GCCGGC              
NciI          527  CCGGG                CC^[GC]GG
              547  CCGGG               
NgoMIV        383  GCCGGC               G^CCGGC
              452  GCCGGC              
NlaIII         91  CATG                 CATG^
              120  CATG                
PleI          235  GACTC                GAGTC(4/5)
               43  GAGTC               
PvuII         347  CAGCTG               CAG^CTG
              826  CAGCTG              
SfiI          455  GGCCATACCGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
              641  GGCCTCTGTGGCC       


#########################################
# 3 OR MORE CUT ENZYMES (   64 enzymes) #
#########################################

AciI        AluI        ApaI        AvaII       BanI        
BanII       BbvI        BfaI        BglI        BmyI        
BsaJI       BsgI        BsiEI       BsiYI       BslI        
BsmFI       Bsp1286I    BsrFI       BsrI        BssKI       
BstNI       Cac8I       CfoI        Cfr10I      Cfr13I      
DdeI        DpnII       DraII       EaeI        EarI        
EcoO109I    EcoRII      EcoT14I     FauI        Fnu4HI      
HaeIII      HapII       HhaI        HinP1I      HinfI       
HpaII       ItaI        Ksp632I     MaeI        MaeII       
MboI        MboII       MnlI        MspA1I      MspI        
MvaI        MwoI        NdeII       NlaIV       PpuMI       
PstI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        StyI        TseI        TspRI       
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