Restriction map for pF1KB7176
 (Linear) (Six-base) MAPSORT of: KIBB7176.seq  Check: 6822  from: 1  to: 1095



KIBB7176.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 10:59  ..

AccI GT'mk_AC

Cuts at:      0     329    1095 
   Size:        329     766
 
Acc65I G'GTAC_C

Cuts at:      0      80    1095 
   Size:         80    1015
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     181     192     483    1095 
   Size:        181      11     291     612

  Fragments arranged by size: 

                 612     291     181      11
 
AleI CACnn'nnGTG

Cuts at:      0     679    1095 
   Size:        679     416
 
AlwNI CAG_nnn'CTG

Cuts at:      0     577     781    1095 
   Size:        577     204     314

  Fragments arranged by size: 

                 577     314     204
 
ApaI G_GGCC'C

Cuts at:      0       8      32     436     719     959    1095 
   Size:          8      24     404     283     240     136

  Fragments arranged by size: 

                 404     283     240     136      24       8
 
AvaI C'yCGr_G

Cuts at:      0     950    1043    1095 
   Size:        950      93      52

  Fragments arranged by size: 

                 950      93      52
 
AvrII C'CTAG_G

Cuts at:      0     739    1095 
   Size:        739     356
 
BanI G'GyrC_C

Cuts at:      0      80     279     537     609     769     879     999    1095 
   Size:         80     199     258      72     160     110     120      96

  Fragments arranged by size: 

                 258     199     160     120     110      96      80      72
            
 
BanII G_rGCy'C

Cuts at:      0       8      32     436     719     959    1095 
   Size:          8      24     404     283     240     136

  Fragments arranged by size: 

                 404     283     240     136      24       8
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     221    1095 
   Size:        221     874
 
BbvCI CC'TCA_GC

Cuts at:      0     350    1095 
   Size:        350     745
 
BglI GCCn_nnn'nGGC

Cuts at:      0     462     648     963    1095 
   Size:        462     186     315     132

  Fragments arranged by size: 

                 462     315     186     132
 
Bme1580I G_kGCm'C

Cuts at:      0       8      32     284     436     540     614     719     772 
   Size:          8      24     252     152     104      74     105      53

Cuts at:    772     959    1095 
   Size:        187     136

  Fragments arranged by size: 

                 252     187     152     136     105     104      74      53
                  24       8
 
BmgBI CAC'GTC

Cuts at:      0     333    1095 
   Size:        333     762
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     464     482     565     897     939    1095 
   Size:        464      18      83     332      42     156

  Fragments arranged by size: 

                 464     332     156      83      42      18
 
BmtI G_CTAG'C

Cuts at:      0     556    1095 
   Size:        556     539
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0      66    1095 
   Size:         66    1029
 
Bpu10I CC'TnA_GC

Cuts at:      0     350    1095 
   Size:        350     745
 
BsaAI yAC'GTr

Cuts at:      0     441    1095 
   Size:        441     654
 
BsaHI Gr'CG_yC

Cuts at:      0     983    1095 
   Size:        983     112
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     241     271     796     826    1095 
   Size:        241      30     525      30     269

  Fragments arranged by size: 

                 525     269     241      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      81     616    1095 
   Size:         81     535     479

  Fragments arranged by size: 

                 535     479      81
 
BseYI C'CCAG_C

Cuts at:      0      53     345    1095 
   Size:         53     292     750

  Fragments arranged by size: 

                 750     292      53
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     430     718    1095 
   Size:        430     288     377

  Fragments arranged by size: 

                 430     377     288
 
BsiEI CG_ry'CG

Cuts at:      0     329     453     466    1095 
   Size:        329     124      13     629

  Fragments arranged by size: 

                 629     329     124      13
 
Bsp1286I G_dGCh'C

Cuts at:      0       8      32     284     436     540     614     719     772 
   Size:          8      24     252     152     104      74     105      53

Cuts at:    772     959    1095 
   Size:        187     136

  Fragments arranged by size: 

                 252     187     152     136     105     104      74      53
                  24       8
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      92    1095 
   Size:         92    1003
 
BsrBI CCG'CTC

Cuts at:      0     145    1095 
   Size:        145     950
 
BsrFI r'CCGG_y

Cuts at:      0     383     452     461    1095 
   Size:        383      69       9     634

  Fragments arranged by size: 

                 634     383      69       9
 
BssSI C'ACGA_G

Cuts at:      0     675    1095 
   Size:        675     420
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     429     681    1095 
   Size:        429     252     414

  Fragments arranged by size: 

                 429     414     252
 
DraIII CAC_nnn'GTG

Cuts at:      0     490    1095 
   Size:        490     605
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    1030    1095 
   Size:       1030      65
 
EaeI y'GGCC_r

Cuts at:      0     116     450     454     463     544    1095 
   Size:        116     334       4       9      81     551

  Fragments arranged by size: 

                 551     334     116      81       9       4
 
EagI C'GGCC_G

Cuts at:      0     450     463    1095 
   Size:        450      13     632

  Fragments arranged by size: 

                 632     450      13
 
EarI CTCTTCn'nnn_

Cuts at:      0      70     636     994    1095 
   Size:         70     566     358     101

  Fragments arranged by size: 

                 566     358     101      70
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     181     192     483    1095 
   Size:        181      11     291     612

  Fragments arranged by size: 

                 612     291     181      11
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     207    1064    1095 
   Size:        207     857      31

  Fragments arranged by size: 

                 857     207      31
 
EcoO109I rG'GnC_Cy

Cuts at:      0       4       5      28      29     288     426     433     716 
   Size:          4       1      23       1     259     138       7     283

Cuts at:    716     743     809     955     956    1047    1095 
   Size:         27      66     146       1      91      48

  Fragments arranged by size: 

                 283     259     146     138      91      66      48      27
                  23       7       4       1       1       1
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     333    1095 
   Size:        333     762
 
FseI GG_CCGG'CC

Cuts at:      0     456    1095 
   Size:        456     639
 
HaeII r_GCGC'y

Cuts at:      0     358     390    1095 
   Size:        358      32     705

  Fragments arranged by size: 

                 705     358      32
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      28      60     165     197    1095 
   Size:         28      32     105      32     898

  Fragments arranged by size: 

                 898     105      32      32      28
 
HincII GTy'rAC

Cuts at:      0     330     914    1095 
   Size:        330     584     181

  Fragments arranged by size: 

                 584     330     181
 
KpnI G_GTAC'C

Cuts at:      0      84    1095 
   Size:         84    1011
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     284    1095 
   Size:        284     811
 
MscI TGG'CCA

Cuts at:      0     118    1095 
   Size:        118     977
 
MslI CAynn'nnrTG

Cuts at:      0     679    1095 
   Size:        679     416
 
MspA1I CmG'CkG

Cuts at:      0      14     349     797     828    1095 
   Size:         14     335     448      31     267

  Fragments arranged by size: 

                 448     335     267      31      14
 
NaeI GCC'GGC

Cuts at:      0     385     454    1095 
   Size:        385      69     641

  Fragments arranged by size: 

                 641     385      69
 
NgoMIV G'CCGG_C

Cuts at:      0     383     452    1095 
   Size:        383      69     643

  Fragments arranged by size: 

                 643     383      69
 
NheI G'CTAG_C

Cuts at:      0     552    1095 
   Size:        552     543
 
PasI CC'CwG_GG

Cuts at:      0     961    1095 
   Size:        961     134
 
PpuMI rG'GwC_Cy

Cuts at:      0     288     426    1047    1095 
   Size:        288     138     621      48

  Fragments arranged by size: 

                 621     288     138      48
 
PspOMI G'GGCC_C

Cuts at:      0       4      28     432     715     955    1095 
   Size:          4      24     404     283     240     140

  Fragments arranged by size: 

                 404     283     240     140      24       4
 
PstI C_TGCA'G

Cuts at:      0      13     368     582     767    1095 
   Size:         13     355     214     185     328

  Fragments arranged by size: 

                 355     328     214     185      13
 
PvuII CAG'CTG

Cuts at:      0     349     828    1095 
   Size:        349     479     267

  Fragments arranged by size: 

                 479     349     267
 
SalI G'TCGA_C

Cuts at:      0     328    1095 
   Size:        328     767
 
SbfI CC_TGCA'GG

Cuts at:      0     582    1095 
   Size:        582     513
 
SfcI C'TryA_G

Cuts at:      0       9     364     578     763    1095 
   Size:          9     355     214     185     332

  Fragments arranged by size: 

                 355     332     214     185       9
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0     462     648    1095 
   Size:        462     186     447

  Fragments arranged by size: 

                 462     447     186
 
StuI AGG'CCT

Cuts at:      0     642    1095 
   Size:        642     453
 
StyI C'CwwG_G

Cuts at:      0     128     284     308     422     739     875     993    1095 
   Size:        128     156      24     114     317     136     118     102

  Fragments arranged by size: 

                 317     156     136     128     118     114     102      24
            
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     501     903    1020    1095 
   Size:        501     402     117      75

  Fragments arranged by size: 

                 501     402     117      75
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     238     270     762     794     796     828     882     914 
   Size:        238      32     492      32       2      32      54      32

Cuts at:    914    1095 
   Size:        181

  Fragments arranged by size: 

                 492     238     181      54      32      32      32      32
                   2
 
XbaI T'CTAG_A

Cuts at:      0     978    1095 
   Size:        978     117
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     126    1095 
   Size:        126     969
 

 Enzymes that do cut:

     AccI   Acc65I     AcuI     AleI    AlwNI     ApaI     AvaI    AvrII
     BanI    BanII     BbsI    BbvCI     BglI Bme1580I    BmgBI     BmrI
     BmtI     BplI   Bpu10I    BsaAI    BsaHI    BsaXI    BseRI    BseYI
     BsgI    BsiEI Bsp1286I    BspMI    BsrBI    BsrFI    BssSI    BstXI
   DraIII     DrdI     EaeI     EagI     EarI  Eco57MI    EcoNI EcoO109I
     FalI     FseI    HaeII    Hin4I   HincII     KpnI     MmeI     MscI
     MslI   MspA1I     NaeI   NgoMIV     NheI     PasI    PpuMI   PspOMI
     PstI    PvuII     SalI     SbfI     SfcI     SfiI     StuI     StyI
    TaqII     TstI     XbaI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AclI     AfeI    AflII   AflIII     AgeI     AhdI
     AlfI     AloI    ApaLI     ApoI     AscI     AseI    AsiSI     BaeI
    BamHI     BbeI     BcgI    BciVI     BclI    BfrBI    BglII     BlpI
     BpmI    BpuEI     BsaI    BsaBI    BsaWI  BsiHKAI    BsiWI     BsmI
    BsmBI    BspEI    BspHI    BsrDI    BsrGI   BssHII   BstAPI    BstBI
   BstEII    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI
    CspCI     DraI     EciI  EcoICRI    EcoRI    EcoRV     FspI    FspAI
  HindIII     HpaI     KasI     MfeI     MluI     NarI     NcoI     NdeI
     NotI     NruI     NsiI     NspI     PacI     PciI    PflMI     PfoI
     PmeI     PmlI     PpiI    PshAI     PsiI    PspXI     PsrI     PvuI
    RsrII     SacI    SacII    SanDI     SapI     ScaI    SexAI     SfoI
     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI
     SspI     SwaI     TatI  Tth111I     XhoI     XmaI     XmnI     ZraI
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