Restriction map for pF1KB7157
##################################
# NO CUT ENZYMES (  133 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AclI        
AcyI        AfaI        AflII       AflIII      AgeI        
AocI        Aor51HI     ApaI        AscI        AseI        
AsnI        Asp700I     Asp718I     AviII       BamHI       
BanII       BbeI        BbrPI       BbvCI       BciVI       
BclI        BcnI        BfrI        BsaAI       BsaBI       
BsaHI       BsaI        BsgI        BsiEI       BsiWI       
BsmBI       Bsp1407I    BspDI       BspHI       BspLU11I    
BsrDI       BsrFI       BsrGI       BssHII      BssSI       
Bst1107I    BstAPI      BstBI       BstUI       BstXI       
BstZ17I     Bsu36I      Cfr10I      ClaI        CpoI        
DraI        DrdI        EagI        EclXI       Eco47III    
Eco52I      Eco81I      EcoNI       EcoRI       EcoRV       
EcoT22I     FauI        FbaI        FseI        FspI        
HaeII       HgaI        Hin1I       HindIII     HpaI        
KasI        KpnI        KspI        MamI        MfeI        
MluI        MseI        MunI        MvnI        NaeI        
NarI        NciI        NdeI        NgoMIV      NheI        
NotI        NruI        NsiI        NspV        PacI        
PaeR7I      PinAI       PleI        PmaCI       PmeI        
PmlI        PshAI       PshBI       Psp1406I    PstI        
PvuI        RcaI        RsaI        RsrII       SacI        
SacII       SalI        SapI        SbfI        ScaI        
SfcI        SfoI        SfuI        SgrAI       SmaI        
SmlI        SnaBI       Sse8387I    SspBI       SspI        
SwaI        TaqI        Tru9I       TthHB8I     XhoI        
XmaCI       XmaI        XmnI        


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# ONE CUT ENZYMES (   53 enzymes) #
###################################

AccIII        607  TCCGGA               T^CCGGA
AcsI          276  GAATTT               [GA]^AATT[CT]
AhdI          516  GACTTGCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I        117  GTGCAC               G^TGCAC
ApaLI         117  GTGCAC               G^TGCAC
ApoI          276  GAATTT               [GA]^AATT[CT]
AspEI         516  GACTTGCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI          823  GACCAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaI          996  CCCGAG               C^[CT]CG[GA]G
AvrII         738  CCTAGG               C^CTAGG
BanI          690  GGTGCC               G^G[CT][GA]CC
BcgI          391  CGATACCTCTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI          787  GCCCTCATGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII        1059  AGATCT               A^GATCT
BlnI          738  CCTAGG               C^CTAGG
BlpI          573  GCTGAGC              GC^T[ACGT]AGC
BpmI          145  CTGGAG               CTGGAG(16/14)
Bpu1102I      573  GCTGAGC              GC^T[ACGT]AGC
BsaWI         607  TCCGGA               [AT]^CCGG[AT]
BseAI         607  TCCGGA               T^CCGGA
BsmI            7  GAATGC               GAATGC(1/-1)
BsoBI         996  CCCGAG               C^[CT]CG[GA]G
BspEI         607  TCCGGA               T^CCGGA
BsrBI         918  CCGCTC               CCGCTC(-3/-3)
BstEII        453  GGTGACC              G^GT[ACGT]ACC
BstPI         453  GGTGACC              G^GT[ACGT]ACC
CelII         573  GCTGAGC              GC^T[ACGT]AGC
CfoI          405  GCGC                 GCG^C
DraIII       1038  CACTCCGTG            CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I      516  GACTTGCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI          122  CTCTTC               CTCTTC(1/4)
EcoO65I       453  GGTGACC              G^GT[ACGT]ACC
HhaI          405  GCGC                 GCG^C
HinP1I        405  GCGC                 G^CGC
HincII        880  GTCAAC               GT[CT]^[GA]AC
HindII        880  GTCAAC               GT[CT]^[GA]AC
Ksp632I       122  CTCTTC               CTCTTC(1/4)
MaeII         152  ACGT                 A^CGT
MroI          607  TCCGGA               T^CCGGA
MslI          649  CATCAGCATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I        101  CAGCTG               C[AC]G^C[GT]G
PflFI         823  GACCAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI         473  GGGTCCT              [GA]G^G[AT]CC[CT]
PvuII         101  CAGCTG               CAG^CTG
SexAI         824  ACCAGGT              A^CC[AT]GGT
SfaNI         464  GCATC                GCATC(5/9)
SfiI          786  GGCCCTCATGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SpeI          486  ACTAGT               A^CTAGT
SphI          262  GCATGC               GCATG^C
StuI          836  AGGCCT               AGG^CCT
Tsp509I       277  AATT                 ^AATT
Tth111I       823  GACCAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XbaI         1062  TCTAGA               T^CTAGA


###################################
# TWO CUT ENZYMES (   13 enzymes) #
###################################

AvaII          88  GGTCC                G^G[AT]CC
              474  GGTCC               
BbsI           96  GAAGAC               GAAGAC(2/6)
              313  GTCTTC              
BpuAI          96  GAAGAC               GAAGAC(2/6)
              313  GTCTTC              
BseRI         760  CTCCTC               GAGGAG(10/8)
              921  CTCCTC              
BsmAI        1015  GAGAC                GTCTC(1/5)
              490  GTCTC               
BsmFI          89  GTCCC                GGGAC(10/14)
              524  GTCCC               
HapII         195  CCGG                 C^CGG
              608  CCGG                
HpaII         195  CCGG                 C^CGG
              608  CCGG                
MspI          195  CCGG                 C^CGG
              608  CCGG                
NcoI          291  CCATGG               C^CATGG
              875  CCATGG              
PflMI         291  CCATGGTGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              798  CCACAGCCTGG         
Van91I        291  CCATGGTGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              798  CCACAGCCTGG         
XcmI           38  CCAAGGATGGCTTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
              282  CCATGTTTTCCATGG     


#########################################
# 3 OR MORE CUT ENZYMES (   59 enzymes) #
#########################################

AciI        AluI        AlwI        AlwNI       AspHI       
BalI        BbvI        BfaI        BmyI        BsaJI       
BsiHKAI     BsiYI       BslI        Bsp1286I    BspMI       
BsrI        BssKI       BstF5I      BstNI       BstYI       
Cac8I       Cfr13I      DdeI        DpnII       DraII       
DsaI        EaeI        EcoO109I    EcoRII      EcoT14I     
Fnu4HI      FokI        HaeIII      HinfI       HphI        
ItaI        MaeI        MaeIII      MboI        MboII       
MflI        MluNI       MnlI        MscI        MvaI        
MwoI        NdeII       NlaIII      NlaIV       NspI        
Sau3AI      Sau96I      ScrFI       StyI        TfiI        
TseI        Tsp45I      TspRI       XhoII       
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