Restriction map for pF1KB7157
 (Linear) (Six-base) MAPSORT of: KIBB7157.seq  Check: 9808  from: 1  to: 1080



KIBB7157.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 10:59  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     678    1080 
   Size:        678     402
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     343    1080 
   Size:        343     737
 
AhdI GACnn_n'nnGTC

Cuts at:      0     521    1080 
   Size:        521     559
 
AlwNI CAG_nnn'CTG

Cuts at:      0     358     533    1024    1080 
   Size:        358     175     491      56

  Fragments arranged by size: 

                 491     358     175      56
 
ApaLI G'TGCA_C

Cuts at:      0     117    1080 
   Size:        117     963
 
ApoI r'AATT_y

Cuts at:      0     276    1080 
   Size:        276     804
 
AvaI C'yCGr_G

Cuts at:      0     996    1080 
   Size:        996      84
 
AvrII C'CTAG_G

Cuts at:      0     738    1080 
   Size:        738     342
 
BanI G'GyrC_C

Cuts at:      0     690    1080 
   Size:        690     390
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     103     306    1080 
   Size:        103     203     774

  Fragments arranged by size: 

                 774     203     103
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     380     414    1080 
   Size:        380      34     666

  Fragments arranged by size: 

                 666     380      34
 
BglI GCCn_nnn'nGGC

Cuts at:      0     793    1080 
   Size:        793     287
 
BglII A'GATC_T

Cuts at:      0    1059    1080 
   Size:       1059      21
 
BlpI GC'TnA_GC

Cuts at:      0     574    1080 
   Size:        574     506
 
Bme1580I G_kGCm'C

Cuts at:      0     121     144     450    1080 
   Size:        121      23     306     630

  Fragments arranged by size: 

                 630     306     121      23
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     772    1080 
   Size:        772     308
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     936    1080 
   Size:        936     144
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     166    1080 
   Size:        166     914
 
BsaWI w'CCGG_w

Cuts at:      0     607    1080 
   Size:        607     473
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     751     912    1080 
   Size:        751     161     168

  Fragments arranged by size: 

                 751     168     161
 
BseYI C'CCAG_C

Cuts at:      0      11     229    1080 
   Size:         11     218     851

  Fragments arranged by size: 

                 851     218      11
 
BsiHKAI G_wGCw'C

Cuts at:      0     121     164     761    1080 
   Size:        121      43     597     319

  Fragments arranged by size: 

                 597     319     121      43
 
BsmI GAATG_Cn'

Cuts at:      0      13    1080 
   Size:         13    1067
 
Bsp1286I G_dGCh'C

Cuts at:      0     121     144     164     450     761    1080 
   Size:        121      23      20     286     311     319

  Fragments arranged by size: 

                 319     311     286     121      23      20
 
BspEI T'CCGG_A

Cuts at:      0     607    1080 
   Size:        607     473
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     506     578     678    1080 
   Size:        506      72     100     402

  Fragments arranged by size: 

                 506     402     100      72
 
BsrBI CCG'CTC

Cuts at:      0     920    1080 
   Size:        920     160
 
BstEII G'GTnAC_C

Cuts at:      0     453    1080 
   Size:        453     627
 
BstYI r'GATC_y

Cuts at:      0     914    1000    1059    1080 
   Size:        914      86      59      21

  Fragments arranged by size: 

                 914      86      59      21
 
BtgI C'CryG_G

Cuts at:      0     291     438     875    1042    1080 
   Size:        291     147     437     167      38

  Fragments arranged by size: 

                 437     291     167     147      38
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     576    1080 
   Size:        576     504
 
BtsI GCAGTG_nn'

Cuts at:      0     933    1080 
   Size:        933     147
 
DraIII CAC_nnn'GTG

Cuts at:      0    1043    1080 
   Size:       1043      37
 
EaeI y'GGCC_r

Cuts at:      0     245     659     672     728     806    1045    1080 
   Size:        245     414      13      56      78     239      35

  Fragments arranged by size: 

                 414     245     239      78      56      35      13
 
EarI CTCTTCn'nnn_

Cuts at:      0     128    1080 
   Size:        128     952
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     181    1080 
   Size:        181     899
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     166     343    1080 
   Size:        166     177     737

  Fragments arranged by size: 

                 737     177     166
 
EcoO109I rG'GnC_Cy

Cuts at:      0     474     530     966    1080 
   Size:        474      56     436     114

  Fragments arranged by size: 

                 474     436     114      56
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0      63     330     855    1080 
   Size:         63     267     525     225

  Fragments arranged by size: 

                 525     267     225      63
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     507     539     992    1024    1080 
   Size:        507      32     453      32      56

  Fragments arranged by size: 

                 507     453      56      32      32
 
HincII GTy'rAC

Cuts at:      0     882    1080 
   Size:        882     198
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      80    1080 
   Size:         80    1000
 
MscI TGG'CCA

Cuts at:      0     247     661     674     730     808    1047    1080 
   Size:        247     414      13      56      78     239      33

  Fragments arranged by size: 

                 414     247     239      78      56      33      13
 
MslI CAynn'nnrTG

Cuts at:      0     653    1080 
   Size:        653     427
 
MspA1I CmG'CkG

Cuts at:      0     103    1080 
   Size:        103     977
 
NcoI C'CATG_G

Cuts at:      0     291     875    1080 
   Size:        291     584     205

  Fragments arranged by size: 

                 584     291     205
 
NspI r_CATG'y

Cuts at:      0     266     634     658    1080 
   Size:        266     368      24     422

  Fragments arranged by size: 

                 422     368     266      24
 
PasI CC'CwG_GG

Cuts at:      0     459    1080 
   Size:        459     621
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     297     804    1080 
   Size:        297     507     276

  Fragments arranged by size: 

                 507     297     276
 
PpuMI rG'GwC_Cy

Cuts at:      0     474    1080 
   Size:        474     606
 
PvuII CAG'CTG

Cuts at:      0     103    1080 
   Size:        103     977
 
SexAI A'CCwGG_T

Cuts at:      0     824    1080 
   Size:        824     256
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0     793    1080 
   Size:        793     287
 
SpeI A'CTAG_T

Cuts at:      0     486    1080 
   Size:        486     594
 
SphI G_CATG'C

Cuts at:      0     266    1080 
   Size:        266     814
 
StuI AGG'CCT

Cuts at:      0     838    1080 
   Size:        838     242
 
StyI C'CwwG_G

Cuts at:      0      38     291     305     738     875     969    1080 
   Size:         38     253      14     433     137      94     111

  Fragments arranged by size: 

                 433     253     137     111      94      38      14
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0       3     404    1080 
   Size:          3     401     676

  Fragments arranged by size: 

                 676     401       3
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     942     974    1080 
   Size:        942      32     106

  Fragments arranged by size: 

                 942     106      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     826    1080 
   Size:        826     254
 
XbaI T'CTAG_A

Cuts at:      0    1062    1080 
   Size:       1062      18
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0      45     289    1080 
   Size:         45     244     791

  Fragments arranged by size: 

                 791     244      45
 

 Enzymes that do cut:

     AarI     AcuI     AhdI    AlwNI    ApaLI     ApoI     AvaI    AvrII
     BanI     BbsI     BcgI     BglI    BglII     BlpI Bme1580I     BmrI
     BplI     BpmI    BsaWI    BseRI    BseYI  BsiHKAI     BsmI Bsp1286I
    BspEI    BspMI    BsrBI   BstEII    BstYI     BtgI    BtgZI     BtsI
   DraIII     EaeI     EarI     EciI  Eco57MI EcoO109I     FalI    Hin4I
   HincII     MmeI     MscI     MslI   MspA1I     NcoI     NspI     PasI
    PflMI    PpuMI    PvuII    SexAI     SfiI     SpeI     SphI     StuI
     StyI    TaqII     TstI  Tth111I     XbaI     XcmI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AleI     AlfI     AloI     ApaI     AscI     AseI    AsiSI     BaeI
    BamHI    BanII     BbeI    BbvCI    BciVI     BclI    BfrBI    BmgBI
     BmtI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaXI
     BsgI    BsiEI    BsiWI    BsmBI    BspHI    BsrDI    BsrFI    BsrGI
   BssHII    BssSI   BstAPI    BstBI    BstXI  BstZ17I   Bsu36I     ClaI
    CspCI     DraI     DrdI     EagI  EcoICRI    EcoNI    EcoRI    EcoRV
     FseI     FspI    FspAI    HaeII  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     NaeI     NarI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     PacI     PciI     PfoI     PmeI     PmlI     PpiI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    RsrII
     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI     SfcI
     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SrfI     SspI
     SwaI     TatI     XhoI     XmaI     XmnI     ZraI
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