Restriction map for pF1KB7149
##################################
# NO CUT ENZYMES (  137 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AflII       AflIII      AgeI        Alw44I      AlwNI       
Aor51HI     ApaI        ApaLI       AscI        AseI        
AsnI        Asp700I     Asp718I     AspI        AviII       
AvrII       BamHI       BbeI        BbrPI       BbvCI       
BcgI        BclI        BcnI        BfrI        BlnI        
BlpI        BpmI        Bpu1102I    BsaBI       BsaWI       
BseAI       BsiEI       BsiWI       BsmBI       Bsp1407I    
BspDI       BspEI       BspLU11I    BsrBI       BsrFI       
BsrGI       BssHII      Bst1107I    BstAPI      BstBI       
BstEII      BstPI       BstXI       BstZ17I     CelII       
Cfr10I      ClaI        CpoI        DraI        DraIII      
DrdI        DsaI        EagI        EclXI       Eco47III    
Eco52I      EcoO65I     EcoRI       EcoRV       EcoT22I     
FauI        FbaI        FseI        FspI        HaeII       
HapII       HincII      HindII      HindIII     HpaI        
HpaII       KasI        KpnI        KspI        MamI        
MfeI        MluI        MroI        MspI        MunI        
NaeI        NarI        NciI        NcoI        NdeI        
NgoMIV      NheI        NotI        NruI        NsiI        
NspI        NspV        PacI        PflFI       PinAI       
PleI        PmaCI       PmeI        PmlI        PshAI       
PshBI       Psp1406I    PvuI        RsrII       SacII       
SalI        SapI        SbfI        ScaI        SexAI       
SfiI        SfoI        SfuI        SgrAI       SmaI        
SpeI        SphI        Sse8387I    SspBI       SspI        
StuI        SwaI        Tth111I     XcmI        XmaCI       
XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   46 enzymes) #
###################################

AcsI          664  AAATTC               [GA]^AATT[CT]
AcyI          971  GGCGTC               G[GA]^CG[CT]C
AhdI           63  GACCCAGGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI          181  CCTCAGG              CC^T[ACGT]AGG
ApoI          664  AAATTC               [GA]^AATT[CT]
AspEI          63  GACCCAGGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbsI          499  GAAGAC               GAAGAC(2/6)
BciVI         138  GGATAC               GTATCC(6/5)
BglI          325  GCCTGGGAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI         499  GAAGAC               GAAGAC(2/6)
BsaAI         359  TACGTA               [CT]AC^GT[GA]
BsaHI         971  GGCGTC               G[GA]^CG[CT]C
BsgI          748  GTGCAG               GTGCAG(16/14)
BsmFI         578  GGGAC                GGGAC(10/14)
BspHI         668  TCATGA               T^CATGA
BsrDI         569  GCAATG               GCAATG(2/0)
BsrI          956  CCAGT                ACTGG(1/-1)
BssSI          32  CTCGTG               CACGAG(-5/-1)
Bsu36I        181  CCTCAGG              CC^T[ACGT]AGG
Cac8I         163  GCTGGC               GC[ACGT]^[ACGT]GC
DraII        1024  AGGTCCT              [GA]G^G[ACGT]CC[CT]
Eam1105I       63  GACCCAGGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I        181  CCTCAGG              CC^T[ACGT]AGG
EcoNI         127  CCTGTTTTAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I     1024  AGGTCCT              [GA]G^G[ACGT]CC[CT]
EcoT14I       255  CCAAGG               C^C[AT][AT]GG
HgaI          972  GCGTC                GACGC(5/10)
Hin1I         971  GGCGTC               G[GA]^CG[CT]C
HinfI         232  GAATC                G^A[ACGT]TC
MaeII         360  ACGT                 A^CGT
MaeIII        893  GTGAC                ^GT[ACGT]AC
MspA1I        215  CAGCTG               C[AC]G^C[GT]G
PaeR7I        856  CTCGAG               C^TCGAG
PflMI         738  CCAAGATCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI        1024  AGGTCCT              [GA]G^G[AT]CC[CT]
PvuII         215  CAGCTG               CAG^CTG
RcaI          668  TCATGA               T^CATGA
SnaBI         359  TACGTA               TAC^GTA
StyI          255  CCAAGG               C^C[AT][AT]GG
TaqI          857  TCGA                 T^CGA
TfiI          232  GAATC                G^A[AT]TC
Tsp45I        893  GTGAC                ^GT[GC]AC
TthHB8I       857  TCGA                 T^CGA
Van91I        738  CCAAGATCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI         1006  TCTAGA               T^CTAGA
XhoI          856  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   32 enzymes) #
###################################

AccII          53  CGCG                 CG^CG
             1001  CGCG                
AciI           45  CCGC                 CCGC(-3/-1)
              465  CCGC                
AvaI            5  CTCGGG               C^[CT]CG[GA]G
              856  CTCGAG              
AvaII         817  GGTCC                G^G[AT]CC
             1025  GGTCC               
BalI          513  TGGCCA               TGG^CCA
              866  TGGCCA              
BfaI          509  CTAG                 C^TAG
             1007  CTAG                
BglII         656  AGATCT               A^GATCT
              741  AGATCT              
BsaI           61  GAGACC               GGTCTC(1/5)
               70  GGTCTC              
BsmAI          61  GAGAC                GTCTC(1/5)
               71  GTCTC               
BsmI          338  GAATGC               GAATGC(1/-1)
              167  GCATTC              
BsoBI           5  CTCGGG               C^[CT]CG[GA]G
              856  CTCGAG              
BspMI         774  GCAGGT               ACCTGC(4/8)
             1022  GCAGGT              
BstF5I        293  CATCC                GGATG(2/0)
              902  CATCC               
BstUI          53  CGCG                 CG^CG
             1001  CGCG                
CfoI           52  GCGC                 GCG^C
             1000  GCGC                
Cfr13I        817  GGTCC                G^G[ACGT]CC
             1025  GGTCC               
EaeI          513  TGGCCA               [CT]^GGCC[GA]
              866  TGGCCA              
FokI          293  CATCC                GGATG(9/13)
              902  CATCC               
HhaI           52  GCGC                 GCG^C
             1000  GCGC                
HinP1I         52  GCGC                 G^CGC
             1000  GCGC                
MaeI          509  CTAG                 C^TAG
             1007  CTAG                
MluNI         513  TGGCCA               TGG^CCA
              866  TGGCCA              
MscI          513  TGGCCA               TGG^CCA
              866  TGGCCA              
MseI          628  TTAA                 T^TAA
              706  TTAA                
MslI          669  CATGAATGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              840  CACCAAGATG          
MvnI           53  CGCG                 CG^CG
             1001  CGCG                
PstI           92  CTGCAG               CTGCA^G
              212  CTGCAG              
Sau96I        817  GGTCC                G^G[ACGT]CC
             1025  GGTCC               
SfaNI         345  GATGC                GCATC(5/9)
              603  GATGC               
SmlI           73  CTCAAG               C^T[CT][GA]AG
              856  CTCGAG              
Tru9I         628  TTAA                 T^TAA
              706  TTAA                
Tsp509I       665  AATT                 ^AATT
              806  AATT                


#########################################
# 3 OR MORE CUT ENZYMES (   43 enzymes) #
#########################################

AfaI        AluI        AlwI        AspHI       BanI        
BanII       BbvI        BmyI        BsaJI       BseRI       
BsiHKAI     BsiYI       BslI        Bsp1286I    BssKI       
BstNI       BstYI       DdeI        DpnII       EarI        
EcoRII      Fnu4HI      HaeIII      HphI        ItaI        
Ksp632I     MboI        MboII       MflI        MnlI        
MvaI        MwoI        NdeII       NlaIII      NlaIV       
RsaI        SacI        Sau3AI      ScrFI       SfcI        
TseI        TspRI       XhoII       
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