Restriction map for pF1KB7149
 (Linear) (Six-base) MAPSORT of: KIBB7149.seq  Check: 4545  from: 1  to: 1041



KIBB7149.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 10:59  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     765    1041 
   Size:        765     276
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     424     784    1041 
   Size:        424     360     257

  Fragments arranged by size: 

                 424     360     257
 
AhdI GACnn_n'nnGTC

Cuts at:      0      68    1041 
   Size:         68     973
 
ApoI r'AATT_y

Cuts at:      0     664    1041 
   Size:        664     377
 
AvaI C'yCGr_G

Cuts at:      0       5     856    1041 
   Size:          5     851     185

  Fragments arranged by size: 

                 851     185       5
 
BanI G'GyrC_C

Cuts at:      0     244     777     880    1041 
   Size:        244     533     103     161

  Fragments arranged by size: 

                 533     244     161     103
 
BanII G_rGCy'C

Cuts at:      0     192     307     383     476     945    1014    1041 
   Size:        192     115      76      93     469      69      27

  Fragments arranged by size: 

                 469     192     115      93      76      69      27
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     506    1041 
   Size:        506     535
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     132    1041 
   Size:        132     909
 
BglI GCCn_nnn'nGGC

Cuts at:      0     331    1041 
   Size:        331     710
 
BglII A'GATC_T

Cuts at:      0     656     741    1041 
   Size:        656      85     300

  Fragments arranged by size: 

                 656     300      85
 
Bme1580I G_kGCm'C

Cuts at:      0     115     782    1041 
   Size:        115     667     259

  Fragments arranged by size: 

                 667     259     115
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     132    1041 
   Size:        132     909
 
Bpu10I CC'TnA_GC

Cuts at:      0     565    1041 
   Size:        565     476
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      58    1041 
   Size:         58     983
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      55      76    1041 
   Size:         55      21     965

  Fragments arranged by size: 

                 965      55      21
 
BsaAI yAC'GTr

Cuts at:      0     361    1041 
   Size:        361     680
 
BsaHI Gr'CG_yC

Cuts at:      0     972    1041 
   Size:        972      69
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      73     108     111     121     712    1041 
   Size:         73      35       3      10     591     329

  Fragments arranged by size: 

                 591     329      73      35      10       3
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     769    1041 
   Size:        769     272
 
BsiHKAI G_wGCw'C

Cuts at:      0      33     192     307     476     704    1041 
   Size:         33     159     115     169     228     337

  Fragments arranged by size: 

                 337     228     169     159     115      33
 
BsmI GAATG_Cn'

Cuts at:      0     167     344    1041 
   Size:        167     177     697

  Fragments arranged by size: 

                 697     177     167
 
Bsp1286I G_dGCh'C

Cuts at:      0      33     115     192     307     383     476     704     782 
   Size:         33      82      77     115      76      93     228      78

Cuts at:    782     945    1014    1041 
   Size:        163      69      27

  Fragments arranged by size: 

                 228     163     115      93      82      78      77      76
                  69      33      27
 
BspHI T'CATG_A

Cuts at:      0     668    1041 
   Size:        668     373
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     765    1013    1041 
   Size:        765     248      28

  Fragments arranged by size: 

                 765     248      28
 
BsrDI GCAATG_nn'

Cuts at:      0     576    1041 
   Size:        576     465
 
BssSI C'ACGA_G

Cuts at:      0      32    1041 
   Size:         32    1009
 
BstYI r'GATC_y

Cuts at:      0     315     443     656     741    1041 
   Size:        315     128     213      85     300

  Fragments arranged by size: 

                 315     300     213     128      85
 
Bsu36I CC'TnA_GG

Cuts at:      0     182    1041 
   Size:        182     859
 
BtsI GCAGTG_nn'

Cuts at:      0      89     203    1041 
   Size:         89     114     838

  Fragments arranged by size: 

                 838     114      89
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     492     527    1041 
   Size:        492      35     514

  Fragments arranged by size: 

                 514     492      35
 
EaeI y'GGCC_r

Cuts at:      0     513     866    1041 
   Size:        513     353     175

  Fragments arranged by size: 

                 513     353     175
 
EarI CTCTTCn'nnn_

Cuts at:      0      10     647     908     929    1041 
   Size:         10     637     261      21     112

  Fragments arranged by size: 

                 637     261     112      21      10
 
EcoICRI GAG'CTC

Cuts at:      0     190     305     474    1041 
   Size:        190     115     169     567

  Fragments arranged by size: 

                 567     190     169     115
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     424     784    1041 
   Size:        424     360     257

  Fragments arranged by size: 

                 424     360     257
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     131    1041 
   Size:        131     910
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1025    1041 
   Size:       1025      16
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     450    1041 
   Size:        450     591
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      54      86     886     918     932     964    1041 
   Size:         54      32     800      32      14      32      77

  Fragments arranged by size: 

                 800      77      54      32      32      32      14
 
MscI TGG'CCA

Cuts at:      0     515     868    1041 
   Size:        515     353     173

  Fragments arranged by size: 

                 515     353     173
 
MslI CAynn'nnrTG

Cuts at:      0     673     844    1041 
   Size:        673     171     197

  Fragments arranged by size: 

                 673     197     171
 
MspA1I CmG'CkG

Cuts at:      0     217    1041 
   Size:        217     824
 
PasI CC'CwG_GG

Cuts at:      0      66    1041 
   Size:         66     975
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     744    1041 
   Size:        744     297
 
PpuMI rG'GwC_Cy

Cuts at:      0    1025    1041 
   Size:       1025      16
 
PspXI vC'TCGA_Gb

Cuts at:      0     856    1041 
   Size:        856     185
 
PstI C_TGCA'G

Cuts at:      0      96     216    1041 
   Size:         96     120     825

  Fragments arranged by size: 

                 825     120      96
 
PvuII CAG'CTG

Cuts at:      0     217    1041 
   Size:        217     824
 
SacI G_AGCT'C

Cuts at:      0     192     307     476    1041 
   Size:        192     115     169     565

  Fragments arranged by size: 

                 565     192     169     115
 
SfcI C'TryA_G

Cuts at:      0      92     212     320    1041 
   Size:         92     120     108     721

  Fragments arranged by size: 

                 721     120     108      92
 
SmlI C'TyrA_G

Cuts at:      0      73     856    1041 
   Size:         73     783     185

  Fragments arranged by size: 

                 783     185      73
 
SnaBI TAC'GTA

Cuts at:      0     361    1041 
   Size:        361     680
 
StyI C'CwwG_G

Cuts at:      0     255    1041 
   Size:        255     786
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     210     604     751    1041 
   Size:        210     394     147     290

  Fragments arranged by size: 

                 394     290     210     147
 
TatI w'GTAC_w

Cuts at:      0     422    1041 
   Size:        422     619
 
XbaI T'CTAG_A

Cuts at:      0    1006    1041 
   Size:       1006      35
 
XhoI C'TCGA_G

Cuts at:      0     856    1041 
   Size:        856     185
 

 Enzymes that do cut:

     AarI     AcuI     AhdI     ApoI     AvaI     BanI    BanII     BbsI
    BciVI     BglI    BglII Bme1580I     BplI   Bpu10I    BpuEI     BsaI
    BsaAI    BsaHI    BseRI     BsgI  BsiHKAI     BsmI Bsp1286I    BspHI
    BspMI    BsrDI    BssSI    BstYI   Bsu36I     BtsI    CspCI     EaeI
     EarI  EcoICRI  Eco57MI    EcoNI EcoO109I     FalI    Hin4I     MscI
     MslI   MspA1I     PasI    PflMI    PpuMI    PspXI     PstI    PvuII
     SacI     SfcI     SmlI    SnaBI     StyI    TaqII     TatI     XbaI
     XhoI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI     AseI
    AsiSI    AvrII     BaeI    BamHI     BbeI    BbvCI     BcgI     BclI
    BfrBI     BlpI    BmgBI     BmrI     BmtI     BpmI    BsaBI    BsaWI
    BsaXI    BseYI    BsiEI    BsiWI    BsmBI    BspEI    BsrBI    BsrFI
    BsrGI   BssHII   BstAPI    BstBI   BstEII    BstXI  BstZ17I     BtgI
    BtgZI     ClaI     DraI   DraIII     DrdI     EagI     EciI    EcoRI
    EcoRV     FseI     FspI    FspAI    HaeII   HincII  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     MmeI     NaeI     NarI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI
     PciI     PfoI     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI
     PsrI     PvuI    RsrII    SacII     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI     SpeI
     SphI     SrfI     SspI     StuI     SwaI     TstI  Tth111I     XcmI
     XmaI     XmnI     ZraI
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