Restriction map for pF1KB7147
##################################
# NO CUT ENZYMES (  124 enzymes) #
##################################

AatII       Acc65I      AclI        AcyI        AflII       
AgeI        AhdI        Alw44I      AocI        Aor51HI     
ApaLI       AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AspI        AvaI        AviII       
AvrII       BbeI        BbrPI       BcgI        BfrI        
BglII       BlnI        BlpI        Bpu1102I    BsaBI       
BsaHI       BsaI        BsgI        BsiWI       BsmAI       
BsmBI       BsmI        BsoBI       Bsp1407I    BspDI       
BspLU11I    BsrDI       BsrGI       BssHII      BssSI       
Bst1107I    BstAPI      BstBI       BstEII      BstPI       
BstXI       BstZ17I     Bsu36I      CelII       ClaI        
DraI        DraIII      Eam1105I    Eco47III    Eco81I      
EcoNI       EcoO65I     EcoRV       EcoT14I     EcoT22I     
FseI        FspI        Hin1I       HindIII     HpaI        
KasI        KpnI        KspI        MamI        MfeI        
MseI        MslI        MunI        NarI        NcoI        
NdeI        NotI        NruI        NsiI        NspV        
PacI        PaeR7I      PflFI       PinAI       PleI        
PmaCI       PmeI        PmlI        PshAI       PshBI       
Psp1406I    PvuI        SacI        SacII       SbfI        
ScaI        SexAI       SfaNI       SfiI        SfoI        
SfuI        SgrAI       SmaI        SmlI        SnaBI       
SpeI        Sse8387I    SspBI       SspI        StuI        
StyI        SwaI        Tru9I       Tth111I     XbaI        
XhoI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   65 enzymes) #
###################################

AccII         726  CGCG                 CG^CG
AccIII        518  TCCGGA               T^CCGGA
AcsI          460  GAATTC               [GA]^AATT[CT]
AflIII        725  ACGCGT               A^C[GA][CT]GT
AlwNI         600  CAGCTACTG            CAG[ACGT][ACGT][ACGT]^CTG
ApaI          715  GGGCCC               GGGCC^C
ApoI          460  GAATTC               [GA]^AATT[CT]
AspHI         154  GTGCTC               G[AT]GC[AT]^C
BalI         1014  TGGCCA               TGG^CCA
BamHI         626  GGATCC               G^GATCC
BanI          490  GGCACC               G^G[CT][GA]CC
BbvCI         870  GCTGAGG              CCTCAGC(-5/-2)
BciVI           3  GGATAC               GTATCC(6/5)
BclI          590  TGATCA               T^GATCA
BfaI          532  CTAG                 C^TAG
BglI          546  GCCCGTGCGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI         778  TACGTG               [CT]AC^GT[GA]
BsaWI         518  TCCGGA               [AT]^CCGG[AT]
BseAI         518  TCCGGA               T^CCGGA
BsiEI         376  CGGCCG               CG[GA][CT]^CG
BsiHKAI       154  GTGCTC               G[AT]GC[AT]^C
BspEI         518  TCCGGA               T^CCGGA
BspHI         861  TCATGA               T^CATGA
BsrBI         956  GAGCGG               CCGCTC(-3/-3)
BsrFI         653  GCCGGC               [GA]^CCGG[CT]
BstUI         726  CGCG                 CG^CG
CfoI          132  GCGC                 GCG^C
Cfr10I        653  GCCGGC               [GA]^CCGG[CT]
CpoI          959  CGGACCG              CG^G[AT]CCG
DrdI          814  GACCCCTTTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI          146  CCGTGG               C^C[GA][CT]GG
EagI          376  CGGCCG               C^GGCCG
EclXI         376  CGGCCG               C^GGCCG
Eco52I        376  CGGCCG               C^GGCCG
EcoRI         460  GAATTC               G^AATTC
FbaI          590  TGATCA               T^GATCA
HaeII         131  AGCGCC               [GA]GCGC^[CT]
HhaI          132  GCGC                 GCG^C
HinP1I        132  GCGC                 G^CGC
HincII        811  GTCGAC               GT[CT]^[GA]AC
HindII        811  GTCGAC               GT[CT]^[GA]AC
HinfI         275  GATTC                G^A[ACGT]TC
MaeI          532  CTAG                 C^TAG
MaeIII        781  GTGAC                ^GT[ACGT]AC
MluI          725  ACGCGT               A^CGCGT
MluNI        1014  TGGCCA               TGG^CCA
MroI          518  TCCGGA               T^CCGGA
MscI         1014  TGGCCA               TGG^CCA
MvnI          726  CGCG                 CG^CG
NaeI          653  GCCGGC               GCC^GGC
NgoMIV        653  GCCGGC               G^CCGGC
NheI          531  GCTAGC               G^CTAGC
NspI          233  GCATGC               [GA]CATG^[CT]
PpuMI         499  GGGACCT              [GA]G^G[AT]CC[CT]
RcaI          861  TCATGA               T^CATGA
RsrII         959  CGGACCG              CG^G[AT]CCG
SalI          811  GTCGAC               G^TCGAC
SapI          946  GAAGAGC              GCTCTTC(1/4)
SphI          233  GCATGC               GCATG^C
TaqI          812  TCGA                 T^CGA
TfiI          275  GATTC                G^A[AT]TC
Tsp45I        781  GTGAC                ^GT[GC]AC
Tsp509I       461  AATT                 ^AATT
TthHB8I       812  TCGA                 T^CGA
XcmI          486  CCAGGGCACCAATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   18 enzymes) #
###################################

AfaI           60  GTAC                 GT^AC
              366  GTAC                
BbsI           49  GTCTTC               GAAGAC(2/6)
              112  GTCTTC              
BpmI          511  CTGGAG               CTGGAG(16/14)
              556  CTGGAG              
BpuAI          49  GTCTTC               GAAGAC(2/6)
              112  GTCTTC              
BstF5I        255  CATCC                GGATG(2/0)
              537  CATCC               
BstYI         626  GGATCC               [GA]^GATC[CT]
              773  GGATCT              
EarI          310  CTCTTC               CTCTTC(1/4)
              946  GAAGAG              
FauI          585  CCCGC                CCCGC(4/6)
              670  GCGGG               
FokI          255  CATCC                GGATG(9/13)
              537  CATCC               
HgaI          783  GACGC                GACGC(5/10)
              727  GCGTC               
Ksp632I       310  CTCTTC               CTCTTC(1/4)
              946  GAAGAG              
MflI          626  GGATCC               [GA]^GATC[CT]
              773  GGATCT              
PflMI         265  CCACTCTCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              732  CCATGTCGTGG         
PstI          127  CTGCAG               CTGCA^G
              438  CTGCAG              
PvuII         320  CAGCTG               CAG^CTG
              968  CAGCTG              
RsaI           60  GTAC                 GT^AC
              366  GTAC                
Van91I        265  CCACTCTCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              732  CCATGTCGTGG         
XhoII         626  GGATCC               [GA]^GATC[CT]
              773  GGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   51 enzymes) #
#########################################

AccI        AciI        AluI        AlwI        AvaII       
BanII       BbvI        BcnI        BmyI        BsaJI       
BseRI       BsiYI       BslI        BsmFI       Bsp1286I    
BspMI       BsrI        BssKI       BstNI       Cac8I       
Cfr13I      DdeI        DpnII       DraII       EaeI        
EcoO109I    EcoRII      Fnu4HI      HaeIII      HapII       
HpaII       HphI        ItaI        MaeII       MboI        
MboII       MnlI        MspA1I      MspI        MvaI        
MwoI        NciI        NdeII       NlaIII      NlaIV       
Sau3AI      Sau96I      ScrFI       SfcI        TseI        
TspRI       
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