Restriction map for pF1KB7147
 (Linear) (Six-base) MAPSORT of: KIBB7147.seq  Check: 7838  from: 1  to: 1038



KIBB7147.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 10:59  ..

AccI GT'mk_AC

Cuts at:      0     449     812     824    1038 
   Size:        449     363      12     214

  Fragments arranged by size: 

                 449     363     214      12
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     946    1038 
   Size:        946      92
 
AflIII A'CryG_T

Cuts at:      0     725    1038 
   Size:        725     313
 
AlwNI CAG_nnn'CTG

Cuts at:      0     605    1038 
   Size:        605     433
 
ApaI G_GGCC'C

Cuts at:      0     719    1038 
   Size:        719     319
 
ApoI r'AATT_y

Cuts at:      0     460    1038 
   Size:        460     578
 
BamHI G'GATC_C

Cuts at:      0     626    1038 
   Size:        626     412
 
BanI G'GyrC_C

Cuts at:      0     490    1038 
   Size:        490     548
 
BanII G_rGCy'C

Cuts at:      0      86     719     890    1038 
   Size:         86     633     171     148

  Fragments arranged by size: 

                 633     171     148      86
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      42     105    1038 
   Size:         42      63     933

  Fragments arranged by size: 

                 933      63      42
 
BbvCI CC'TCA_GC

Cuts at:      0     871    1038 
   Size:        871     167
 
BclI T'GATC_A

Cuts at:      0     590    1038 
   Size:        590     448
 
BglI GCCn_nnn'nGGC

Cuts at:      0     552    1038 
   Size:        552     486
 
Bme1580I G_kGCm'C

Cuts at:      0     493     719    1038 
   Size:        493     226     319

  Fragments arranged by size: 

                 493     319     226
 
BmgBI CAC'GTC

Cuts at:      0     153    1038 
   Size:        153     885
 
BmtI G_CTAG'C

Cuts at:      0     535    1038 
   Size:        535     503
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     532     577    1038 
   Size:        532      45     461

  Fragments arranged by size: 

                 532     461      45
 
Bpu10I CC'TnA_GC

Cuts at:      0     871    1038 
   Size:        871     167
 
BsaAI yAC'GTr

Cuts at:      0     780    1038 
   Size:        780     258
 
BsaWI w'CCGG_w

Cuts at:      0     518    1038 
   Size:        518     520
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     817     820     847     850     867     897    1038 
   Size:        817       3      27       3      17      30     141

  Fragments arranged by size: 

                 817     141      30      27      17       3       3
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     675     825     828     888    1038 
   Size:        675     150       3      60     150

  Fragments arranged by size: 

                 675     150     150      60       3
 
BseYI C'CCAG_C

Cuts at:      0     384    1038 
   Size:        384     654
 
BsiEI CG_ry'CG

Cuts at:      0     379    1038 
   Size:        379     659
 
BsiHKAI G_wGCw'C

Cuts at:      0     158    1038 
   Size:        158     880
 
Bsp1286I G_dGCh'C

Cuts at:      0      86     158     493     719     890    1038 
   Size:         86      72     335     226     171     148

  Fragments arranged by size: 

                 335     226     171     148      86      72
 
BspEI T'CCGG_A

Cuts at:      0     518    1038 
   Size:        518     520
 
BspHI T'CATG_A

Cuts at:      0     861    1038 
   Size:        861     177
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     104     191     385     511    1038 
   Size:        104      87     194     126     527

  Fragments arranged by size: 

                 527     194     126     104      87
 
BsrBI CCG'CTC

Cuts at:      0     958    1038 
   Size:        958      80
 
BsrFI r'CCGG_y

Cuts at:      0     653    1038 
   Size:        653     385
 
BstYI r'GATC_y

Cuts at:      0     626     773    1038 
   Size:        626     147     265

  Fragments arranged by size: 

                 626     265     147
 
BtgI C'CryG_G

Cuts at:      0     146    1038 
   Size:        146     892
 
BtsI GCAGTG_nn'

Cuts at:      0     435    1038 
   Size:        435     603
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     820    1038 
   Size:        820     218
 
EaeI y'GGCC_r

Cuts at:      0     143     376     683    1014    1038 
   Size:        143     233     307     331      24

  Fragments arranged by size: 

                 331     307     233     143      24
 
EagI C'GGCC_G

Cuts at:      0     376    1038 
   Size:        376     662
 
EarI CTCTTCn'nnn_

Cuts at:      0     316     941    1038 
   Size:        316     625      97

  Fragments arranged by size: 

                 625     316      97
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     532     577     946    1038 
   Size:        532      45     369      92

  Fragments arranged by size: 

                 532     369      92      45
 
EcoO109I rG'GnC_Cy

Cuts at:      0      10     500     716    1038 
   Size:         10     490     216     322

  Fragments arranged by size: 

                 490     322     216      10
 
EcoRI G'AATT_C

Cuts at:      0     460    1038 
   Size:        460     578
 
HaeII r_GCGC'y

Cuts at:      0     135    1038 
   Size:        135     903
 
HincII GTy'rAC

Cuts at:      0     813    1038 
   Size:        813     225
 
MluI A'CGCG_T

Cuts at:      0     725    1038 
   Size:        725     313
 
MscI TGG'CCA

Cuts at:      0    1016    1038 
   Size:       1016      22
 
MspA1I CmG'CkG

Cuts at:      0     322     588     970    1038 
   Size:        322     266     382      68

  Fragments arranged by size: 

                 382     322     266      68
 
NaeI GCC'GGC

Cuts at:      0     655    1038 
   Size:        655     383
 
NgoMIV G'CCGG_C

Cuts at:      0     653    1038 
   Size:        653     385
 
NheI G'CTAG_C

Cuts at:      0     531    1038 
   Size:        531     507
 
NspI r_CATG'y

Cuts at:      0     237    1038 
   Size:        237     801
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     271     738    1038 
   Size:        271     467     300

  Fragments arranged by size: 

                 467     300     271
 
PpuMI rG'GwC_Cy

Cuts at:      0     500    1038 
   Size:        500     538
 
PspOMI G'GGCC_C

Cuts at:      0     715    1038 
   Size:        715     323
 
PstI C_TGCA'G

Cuts at:      0     131     442    1038 
   Size:        131     311     596

  Fragments arranged by size: 

                 596     311     131
 
PvuII CAG'CTG

Cuts at:      0     322     970    1038 
   Size:        322     648      68

  Fragments arranged by size: 

                 648     322      68
 
RsrII CG'GwC_CG

Cuts at:      0     960    1038 
   Size:        960      78
 
SalI G'TCGA_C

Cuts at:      0     811    1038 
   Size:        811     227
 
SapI GCTCTTCn'nnn_

Cuts at:      0     941    1038 
   Size:        941      97
 
SfcI C'TryA_G

Cuts at:      0     127     438     609    1038 
   Size:        127     311     171     429

  Fragments arranged by size: 

                 429     311     171     127
 
SphI G_CATG'C

Cuts at:      0     237    1038 
   Size:        237     801
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     371     977    1038 
   Size:        371     606      61

  Fragments arranged by size: 

                 606     371      61
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     867     899    1038 
   Size:        867      32     139

  Fragments arranged by size: 

                 867     139      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     493    1038 
   Size:        493     545
 

 Enzymes that do cut:

     AccI     AcuI   AflIII    AlwNI     ApaI     ApoI    BamHI     BanI
    BanII     BbsI    BbvCI     BclI     BglI Bme1580I    BmgBI     BmtI
     BpmI   Bpu10I    BsaAI    BsaWI    BsaXI    BseRI    BseYI    BsiEI
  BsiHKAI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrFI    BstYI
     BtgI     BtsI     DrdI     EaeI     EagI     EarI  Eco57MI EcoO109I
    EcoRI    HaeII   HincII     MluI     MscI   MspA1I     NaeI   NgoMIV
     NheI     NspI    PflMI    PpuMI   PspOMI     PstI    PvuII    RsrII
     SalI     SapI     SfcI     SphI    TaqII     TstI     XcmI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII     AgeI     AhdI
     AleI     AlfI     AloI    ApaLI     AscI     AseI    AsiSI     AvaI
    AvrII     BaeI     BbeI     BcgI    BciVI    BfrBI    BglII     BlpI
     BmrI     BplI    BpuEI     BsaI    BsaBI    BsaHI     BsgI    BsiWI
     BsmI    BsmBI    BsrDI    BsrGI   BssHII    BssSI   BstAPI    BstBI
   BstEII    BstXI  BstZ17I   Bsu36I    BtgZI     ClaI    CspCI     DraI
   DraIII     EciI  EcoICRI    EcoNI    EcoRV     FalI     FseI     FspI
    FspAI    Hin4I  HindIII     HpaI     KasI     KpnI     MfeI     MmeI
     MslI     NarI     NcoI     NdeI     NotI     NruI     NsiI     PacI
     PasI     PciI     PfoI     PmeI     PmlI     PpiI    PshAI     PsiI
    PspXI     PsrI     PvuI     SacI    SacII    SanDI     SbfI     ScaI
    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI
     SpeI     SrfI     SspI     StuI     StyI     SwaI     TatI  Tth111I
     XbaI     XhoI     XmaI     XmnI     ZraI
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