Restriction map for pF1KB7145
##################################
# NO CUT ENZYMES (  182 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AcyI        AflII       AgeI        AhdI        AlwI        
AlwNI       AocI        Aor51HI     ApaI        AscI        
AseI        AsnI        Asp718I     AspEI       AspI        
AvaI        AvaII       AviII       AvrII       BamHI       
BanI        BanII       BbeI        BbrPI       BbsI        
BbvCI       BcgI        BciVI       BcnI        BfrI        
BglI        BglII       BlnI        BlpI        BpmI        
Bpu1102I    BpuAI       BsaAI       BsaBI       BsaHI       
BsaI        BsaJI       BsaWI       BseAI       BseRI       
BsiEI       BsiWI       BsmAI       BsmBI       BsmFI       
BsoBI       BspEI       BspHI       BspMI       BsrBI       
BsrFI       BssHII      BssKI       BssSI       Bst1107I    
BstAPI      BstBI       BstEII      BstF5I      BstNI       
BstPI       BstUI       BstXI       BstYI       BstZ17I     
Bsu36I      CelII       CfoI        Cfr10I      Cfr13I      
CpoI        DraII       DraIII      DrdI        DsaI        
EagI        Eam1105I    EarI        EclXI       Eco47III    
Eco52I      Eco81I      EcoNI       EcoO109I    EcoO65I     
EcoRII      EcoRV       EcoT14I     EcoT22I     FokI        
FseI        FspI        HaeII       HapII       HhaI        
Hin1I       HinP1I      HindIII     HpaII       HphI        
KasI        KpnI        Ksp632I     KspI        MaeII       
MamI        MboII       MflI        MluI        MroI        
MspA1I      MspI        MvaI        MvnI        NaeI        
NarI        NciI        NcoI        NdeI        NgoMIV      
NheI        NlaIV       NotI        NruI        NsiI        
NspV        PacI        PaeR7I      PflFI       PflMI       
PinAI       PmaCI       PmeI        PmlI        PpuMI       
PshAI       PshBI       Psp1406I    PstI        PvuI        
PvuII       RcaI        RsrII       SacI        SacII       
SalI        SapI        Sau96I      SbfI        ScaI        
ScrFI       SexAI       SfiI        SfoI        SfuI        
SgrAI       SmaI        SnaBI       SpeI        SphI        
Sse8387I    SspI        StuI        StyI        SwaI        
Tth111I     Van91I      XbaI        XhoI        XhoII       
XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   40 enzymes) #
###################################

AciI          449  CCGC                 CCGC(-3/-1)
AflIII        296  ACATGT               A^C[GA][CT]GT
AluI           16  AGCT                 AG^CT
Alw44I        443  GTGCAC               G^TGCAC
ApaLI         443  GTGCAC               G^TGCAC
Asp700I       508  GAAAATTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI         443  GTGCAC               G[AT]GC[AT]^C
BalI          244  TGGCCA               TGG^CCA
BclI          704  TGATCA               T^GATCA
BfaI          611  CTAG                 C^TAG
BmyI          443  GTGCAC               G[AGT]GC[ACT]^C
BsgI          962  GTGCAG               GTGCAG(16/14)
BsiHKAI       443  GTGCAC               G[AT]GC[AT]^C
BsiYI         471  CCACTCTCAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI          471  CCACTCTCAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmI          308  GCATTC               GAATGC(1/-1)
Bsp1286I      443  GTGCAC               G[AGT]GC[ACT]^C
Bsp1407I      696  TGTACA               T^GTACA
BspDI         338  ATCGAT               AT^CGAT
BspLU11I      296  ACATGT               A^CATGT
BsrDI         178  GCAATG               GCAATG(2/0)
BsrGI         696  TGTACA               T^GTACA
Cac8I         499  GCCTGC               GC[ACGT]^[ACGT]GC
ClaI          338  ATCGAT               AT^CGAT
EaeI          244  TGGCCA               [CT]^GGCC[GA]
EcoRI         897  GAATTC               G^AATTC
FauI          448  CCCGC                CCCGC(4/6)
FbaI          704  TGATCA               T^GATCA
HaeIII        245  GGCC                 GG^CC
HgaI          131  GCGTC                GACGC(5/10)
MaeI          611  CTAG                 C^TAG
MluNI         244  TGGCCA               TGG^CCA
MscI          244  TGGCCA               TGG^CCA
MslI          411  CACTGGCGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
SfaNI         279  GATGC                GCATC(5/9)
SmlI          543  CTCAAG               C^T[CT][GA]AG
SspBI         696  TGTACA               T^GTACA
Tsp45I        935  GTGAC                ^GT[GC]AC
XcmI          517  CCAGAAGCCACATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI          508  GAAAATTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   19 enzymes) #
###################################

AccI          355  GTCTAC               GT^[AC][GT]AC
              465  GTCTAC              
BbvI          799  GCAGC                GCAGC(8/12)
             1027  GCTGC               
BsrI          412  ACTGG                ACTGG(1/-1)
              917  ACTGG               
DraI          594  TTTAAA               TTT^AAA
              681  TTTAAA              
Fnu4HI        799  GCAGC                GC^[ACGT]GC
             1027  GCTGC               
HincII         10  GTTAAC               GT[CT]^[GA]AC
              423  GTTAAC              
HindII         10  GTTAAC               GT[CT]^[GA]AC
              423  GTTAAC              
HpaI           10  GTTAAC               GTT^AAC
              423  GTTAAC              
ItaI          799  GCAGC                GC^[ACGT]GC
             1027  GCTGC               
MfeI          102  CAATTG               C^AATTG
              350  CAATTG              
MunI          102  CAATTG               C^AATTG
              350  CAATTG              
NspI           65  GCATGT               [GA]CATG^[CT]
              296  ACATGT              
PleI           37  GACTC                GAGTC(4/5)
              375  GACTC               
SfcI          863  CTATAG               C^T[GA][CT]AG
              988  CTACAG              
TaqI          339  TCGA                 T^CGA
              563  TCGA                
TfiI          944  GATTC                G^A[AT]TC
             1021  GAATC               
TseI          799  GCAGC                G^C[AT]GC
             1027  GCTGC               
TspRI          22  CACTG                CA[GC]TG[ACGT][ACGT]^
              411  CACTG               
TthHB8I       339  TCGA                 T^CGA
              563  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   17 enzymes) #
#########################################

AcsI        AfaI        ApoI        DdeI        DpnII       
HinfI       MaeIII      MboI        MnlI        MseI        
MwoI        NdeII       NlaIII      RsaI        Sau3AI      
Tru9I       Tsp509I     
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