Restriction map for pF1KB7137
 (Linear) (Six-base) MAPSORT of: KIBB7137.seq  Check: 7763  from: 1  to: 984



KIBB7137.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 10:59  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     781     984 
   Size:        781     203
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     751     960     984 
   Size:        751     209      24

  Fragments arranged by size: 

                 751     209      24
 
AgeI A'CCGG_T

Cuts at:      0     416     984 
   Size:        416     568
 
AleI CACnn'nnGTG

Cuts at:      0     493     793     984 
   Size:        493     300     191

  Fragments arranged by size: 

                 493     300     191
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     447     984 
   Size:        447     537
 
ApaI G_GGCC'C

Cuts at:      0     168     984 
   Size:        168     816
 
BanII G_rGCy'C

Cuts at:      0     168     218     325     984 
   Size:        168      50     107     659

  Fragments arranged by size: 

                 659     168     107      50
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     745     810     984 
   Size:        745      65     174

  Fragments arranged by size: 

                 745     174      65
 
Bme1580I G_kGCm'C

Cuts at:      0     168     984 
   Size:        168     816
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     920     984 
   Size:        920      64
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     705     984 
   Size:        705     279
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     846     984 
   Size:        846     138
 
BsaAI yAC'GTr

Cuts at:      0     368     984 
   Size:        368     616
 
BsaBI GATnn'nnATC

Cuts at:      0     694     984 
   Size:        694     290
 
BsaWI w'CCGG_w

Cuts at:      0     416     984 
   Size:        416     568
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     385     984 
   Size:        385     599
 
BseYI C'CCAG_C

Cuts at:      0      46     350     984 
   Size:         46     304     634

  Fragments arranged by size: 

                 634     304      46
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     255     984 
   Size:        255     729
 
BsiHKAI G_wGCw'C

Cuts at:      0     113     325     984 
   Size:        113     212     659

  Fragments arranged by size: 

                 659     212     113
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     723     984 
   Size:        723     261
 
Bsp1286I G_dGCh'C

Cuts at:      0     113     168     218     325     984 
   Size:        113      55      50     107     659

  Fragments arranged by size: 

                 659     113     107      55      50
 
BspHI T'CATG_A

Cuts at:      0     644     984 
   Size:        644     340
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     781     984 
   Size:        781     203
 
BsrFI r'CCGG_y

Cuts at:      0     416     984 
   Size:        416     568
 
BsrGI T'GTAC_A

Cuts at:      0     884     984 
   Size:        884     100
 
BstEII G'GTnAC_C

Cuts at:      0      78     984 
   Size:         78     906
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     297     984 
   Size:        297     687
 
Bsu36I CC'TnA_GG

Cuts at:      0     955     984 
   Size:        955      29
 
BtgI C'CryG_G

Cuts at:      0     791     984 
   Size:        791     193
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     681     984 
   Size:        681     303
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     699     984 
   Size:        699     285
 
EaeI y'GGCC_r

Cuts at:      0     194     299     485     521     984 
   Size:        194     105     186      36     463

  Fragments arranged by size: 

                 463     194     186     105      36
 
EarI CTCTTCn'nnn_

Cuts at:      0     154     282     311     783     856     984 
   Size:        154     128      29     472      73     128

  Fragments arranged by size: 

                 472     154     128     128      73      29
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     673     984 
   Size:        673     311
 
EcoICRI GAG'CTC

Cuts at:      0     323     984 
   Size:        323     661
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     705     751     960     984 
   Size:        705      46     209      24

  Fragments arranged by size: 

                 705     209      46      24
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     750     984 
   Size:        750     234
 
EcoO109I rG'GnC_Cy

Cuts at:      0     164     165     594     984 
   Size:        164       1     429     390

  Fragments arranged by size: 

                 429     390     164       1
 
HaeII r_GCGC'y

Cuts at:      0     298     984 
   Size:        298     686
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0       3      35     984 
   Size:          3      32     949

  Fragments arranged by size: 

                 949      32       3
 
HincII GTy'rAC

Cuts at:      0     516     636     984 
   Size:        516     120     348

  Fragments arranged by size: 

                 516     348     120
 
HindIII A'AGCT_T

Cuts at:      0     961     984 
   Size:        961      23
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     293     984 
   Size:        293     691
 
MscI TGG'CCA

Cuts at:      0     196     301     487     984 
   Size:        196     105     186     497

  Fragments arranged by size: 

                 497     196     186     105
 
MslI CAynn'nnrTG

Cuts at:      0     403     493     793     984 
   Size:        403      90     300     191

  Fragments arranged by size: 

                 403     300     191      90
 
MspA1I CmG'CkG

Cuts at:      0     140     441     479     814     984 
   Size:        140     301      38     335     170

  Fragments arranged by size: 

                 335     301     170     140      38
 
NspI r_CATG'y

Cuts at:      0     804     984 
   Size:        804     180
 
PasI CC'CwG_GG

Cuts at:      0     211     984 
   Size:        211     773
 
PflMI CCAn_nnn'nTGG

Cuts at:      0      75     984 
   Size:         75     909
 
PmlI CAC'GTG

Cuts at:      0     368     984 
   Size:        368     616
 
PpuMI rG'GwC_Cy

Cuts at:      0     594     984 
   Size:        594     390
 
PspOMI G'GGCC_C

Cuts at:      0     164     984 
   Size:        164     820
 
PstI C_TGCA'G

Cuts at:      0      98     478     622     984 
   Size:         98     380     144     362

  Fragments arranged by size: 

                 380     362     144      98
 
PvuII CAG'CTG

Cuts at:      0     140     984 
   Size:        140     844
 
SacI G_AGCT'C

Cuts at:      0     325     984 
   Size:        325     659
 
SapI GCTCTTCn'nnn_

Cuts at:      0     783     856     984 
   Size:        783      73     128

  Fragments arranged by size: 

                 783     128      73
 
SbfI CC_TGCA'GG

Cuts at:      0      98     984 
   Size:         98     886
 
SfcI C'TryA_G

Cuts at:      0      94     474     618     849     984 
   Size:         94     380     144     231     135

  Fragments arranged by size: 

                 380     231     144     135      94
 
StyI C'CwwG_G

Cuts at:      0      52     191     290     984 
   Size:         52     139      99     694

  Fragments arranged by size: 

                 694     139      99      52
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     128     984 
   Size:        128     856
 
TatI w'GTAC_w

Cuts at:      0     230     884     984 
   Size:        230     654     100

  Fragments arranged by size: 

                 654     230     100
 
XmnI GAAnn'nnTTC

Cuts at:      0     763     964     984 
   Size:        763     201      20

  Fragments arranged by size: 

                 763     201      20
 

 Enzymes that do cut:

     AarI     AcuI     AgeI     AleI     AlfI     ApaI    BanII     BbsI
 Bme1580I     BplI     BpmI     BsaI    BsaAI    BsaBI    BsaWI    BseRI
    BseYI     BsgI  BsiHKAI    BsmBI Bsp1286I    BspHI    BspMI    BsrFI
    BsrGI   BstEII    BstXI   Bsu36I     BtgI    BtgZI     DrdI     EaeI
     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    HaeII    Hin4I
   HincII  HindIII     MmeI     MscI     MslI   MspA1I     NspI     PasI
    PflMI     PmlI    PpuMI   PspOMI     PstI    PvuII     SacI     SapI
     SbfI     SfcI     StyI    TaqII     TatI     XmnI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII     AhdI
     AloI    AlwNI    ApaLI     ApoI     AscI     AseI    AsiSI     AvaI
    AvrII     BaeI    BamHI     BanI     BbeI    BbvCI     BcgI    BciVI
     BclI    BfrBI     BglI    BglII     BlpI    BmgBI     BmrI     BmtI
   Bpu10I    BpuEI    BsaHI    BsaXI    BsiEI    BsiWI     BsmI    BspEI
    BsrBI    BsrDI   BssHII    BssSI   BstAPI    BstBI    BstYI  BstZ17I
     BtsI     ClaI    CspCI     DraI   DraIII     EagI    EcoRI    EcoRV
     FalI     FseI     FspI    FspAI     HpaI     KasI     KpnI     MfeI
     MluI     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     PacI     PciI     PfoI     PmeI     PpiI    PshAI
     PsiI    PspXI     PsrI     PvuI    RsrII    SacII     SalI    SanDI
     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI
    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI     TstI
  Tth111I     XbaI     XcmI     XhoI     XmaI     ZraI
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