Restriction map for pF1KB7133
 (Linear) (Six-base) MAPSORT of: KIBB7133.seq  Check: 2927  from: 1  to: 957



KIBB7133.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 10:59  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     774     957 
   Size:        774     183
 
AccI GT'mk_AC

Cuts at:      0     551     957 
   Size:        551     406
 
Acc65I G'GTAC_C

Cuts at:      0     889     957 
   Size:        889      68
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     113     957 
   Size:        113     844
 
AhdI GACnn_n'nnGTC

Cuts at:      0      68     957 
   Size:         68     889
 
AleI CACnn'nnGTG

Cuts at:      0     266     957 
   Size:        266     691
 
AlwNI CAG_nnn'CTG

Cuts at:      0     363     584     655     957 
   Size:        363     221      71     302

  Fragments arranged by size: 

                 363     302     221      71
 
ApoI r'AATT_y

Cuts at:      0     673     957 
   Size:        673     284
 
AvaI C'yCGr_G

Cuts at:      0      53      65     957 
   Size:         53      12     892

  Fragments arranged by size: 

                 892      53      12
 
BanI G'GyrC_C

Cuts at:      0     250     786     889     957 
   Size:        250     536     103      68

  Fragments arranged by size: 

                 536     250     103      68
 
BanII G_rGCy'C

Cuts at:      0     195     316     957 
   Size:        195     121     641

  Fragments arranged by size: 

                 641     195     121
 
BglII A'GATC_T

Cuts at:      0     665     750     957 
   Size:        665      85     207

  Fragments arranged by size: 

                 665     207      85
 
Bme1580I G_kGCm'C

Cuts at:      0      33     791     957 
   Size:         33     758     166

  Fragments arranged by size: 

                 758     166      33
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     729     957 
   Size:        729     228
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     793     886     957 
   Size:        793      93      71

  Fragments arranged by size: 

                 793      93      71
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     442     517     957 
   Size:        442      75     440

  Fragments arranged by size: 

                 442     440      75
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      55      76     957 
   Size:         55      21     881

  Fragments arranged by size: 

                 881      55      21
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     721     957 
   Size:        721     236
 
BseYI C'CCAG_C

Cuts at:      0     790     957 
   Size:        790     167
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     376     778     957 
   Size:        376     402     179

  Fragments arranged by size: 

                 402     376     179
 
BsiHKAI G_wGCw'C

Cuts at:      0     443     713     957 
   Size:        443     270     244

  Fragments arranged by size: 

                 443     270     244
 
BsmI GAATG_Cn'

Cuts at:      0     170     957 
   Size:        170     787
 
Bsp1286I G_dGCh'C

Cuts at:      0      33     195     316     443     713     791     957 
   Size:         33     162     121     127     270      78     166

  Fragments arranged by size: 

                 270     166     162     127     121      78      33
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     774     957 
   Size:        774     183
 
BsrGI T'GTAC_A

Cuts at:      0     368     957 
   Size:        368     589
 
BssSI C'ACGA_G

Cuts at:      0       5     957 
   Size:          5     952
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     753     957 
   Size:        753     204
 
BstYI r'GATC_y

Cuts at:      0     324     452     665     750     957 
   Size:        324     128     213      85     207

  Fragments arranged by size: 

                 324     213     207     128      85
 
Bsu36I CC'TnA_GG

Cuts at:      0      83     957 
   Size:         83     874
 
BtsI GCAGTG_nn'

Cuts at:      0     200     801     957 
   Size:        200     601     156

  Fragments arranged by size: 

                 601     200     156
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     802     837     957 
   Size:        802      35     120

  Fragments arranged by size: 

                 802     120      35
 
EaeI y'GGCC_r

Cuts at:      0     522     875     957 
   Size:        522     353      82

  Fragments arranged by size: 

                 522     353      82
 
EarI CTCTTCn'nnn_

Cuts at:      0      10     101     132     656     935     957 
   Size:         10      91      31     524     279      22

  Fragments arranged by size: 

                 524     279      91      31      22      10
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     113     793     886     957 
   Size:        113     680      93      71

  Fragments arranged by size: 

                 680     113      93      71
 
EcoO109I rG'GnC_Cy

Cuts at:      0     111     474     957 
   Size:        111     363     483

  Fragments arranged by size: 

                 483     363     111
 
EcoRI G'AATT_C

Cuts at:      0     673     957 
   Size:        673     284
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      54      86     553     585     957 
   Size:         54      32     467      32     372

  Fragments arranged by size: 

                 467     372      54      32      32
 
HincII GTy'rAC

Cuts at:      0     513     957 
   Size:        513     444
 
HindIII A'AGCT_T

Cuts at:      0     604     926     957 
   Size:        604     322      31

  Fragments arranged by size: 

                 604     322      31
 
KpnI G_GTAC'C

Cuts at:      0     893     957 
   Size:        893      64
 
MscI TGG'CCA

Cuts at:      0     877     957 
   Size:        877      80
 
MslI CAynn'nnrTG

Cuts at:      0     266     957 
   Size:        266     691
 
MspA1I CmG'CkG

Cuts at:      0     208     957 
   Size:        208     749
 
PfoI T'CCnGG_A

Cuts at:      0     863     957 
   Size:        863      94
 
SmaI CCC'GGG

Cuts at:      0      67     957 
   Size:         67     890
 
SmlI C'TyrA_G

Cuts at:      0     457     496     957 
   Size:        457      39     461

  Fragments arranged by size: 

                 461     457      39
 
StuI AGG'CCT

Cuts at:      0     295     957 
   Size:        295     662
 
StyI C'CwwG_G

Cuts at:      0     264     878     957 
   Size:        264     614      79

  Fragments arranged by size: 

                 614     264      79
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     613     957 
   Size:        613     344
 
TatI w'GTAC_w

Cuts at:      0     368     776     957 
   Size:        368     408     181

  Fragments arranged by size: 

                 408     368     181
 
XmaI C'CCGG_G

Cuts at:      0      65     957 
   Size:         65     892
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AcuI     AhdI     AleI    AlwNI     ApoI
     AvaI     BanI    BanII    BglII Bme1580I     BplI     BpmI    BpuEI
     BsaI    BseRI    BseYI     BsgI  BsiHKAI     BsmI Bsp1286I    BspMI
    BsrGI    BssSI    BstXI    BstYI   Bsu36I     BtsI    CspCI     EaeI
     EarI  Eco57MI EcoO109I    EcoRI    Hin4I   HincII  HindIII     KpnI
     MscI     MslI   MspA1I     PfoI     SmaI     SmlI     StuI     StyI
    TaqII     TatI     XmaI

 Enzymes that do not cut: 

    AatII     AclI     AfeI    AflII   AflIII     AgeI     AlfI     AloI
     ApaI    ApaLI     AscI     AseI    AsiSI    AvrII     BaeI    BamHI
     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI
     BlpI    BmgBI     BmrI     BmtI   Bpu10I    BsaAI    BsaBI    BsaHI
    BsaWI    BsaXI    BsiEI    BsiWI    BsmBI    BspEI    BspHI    BsrBI
    BsrDI    BsrFI   BssHII   BstAPI    BstBI   BstEII  BstZ17I     BtgI
    BtgZI     ClaI     DraI   DraIII     DrdI     EagI     EciI  EcoICRI
    EcoNI    EcoRV     FalI     FseI     FspI    FspAI    HaeII     HpaI
     KasI     MfeI     MluI     MmeI     NaeI     NarI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI     PasI
     PciI    PflMI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII     SacI
    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfcI
     SfiI     SfoI     SgfI    SgrAI    SnaBI     SpeI     SphI     SrfI
     SspI     SwaI     TstI  Tth111I     XbaI     XcmI     XhoI     XmnI
     ZraI
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