Restriction map for
pF1KB7129
(Linear) (Six-base) MAPSORT of: KIBB7129.seq Check: 5053 from: 1 to: 948
KIBB7129.orf
With 182 enzymes: SgfI *
March 3, 2008 10:59 ..
AccI GT'mk_AC
Cuts at: 0 767 860 948
Size: 767 93 88
Fragments arranged by size:
767 93 88
AlwNI CAG_nnn'CTG
Cuts at: 0 237 948
Size: 237 711
ApaI G_GGCC'C
Cuts at: 0 256 948
Size: 256 692
BanII G_rGCy'C
Cuts at: 0 256 948
Size: 256 692
BbsI GAAGACnn'nnnn_
Cuts at: 0 78 268 948
Size: 78 190 680
Fragments arranged by size:
680 190 78
BbvCI CC'TCA_GC
Cuts at: 0 670 948
Size: 670 278
Bme1580I G_kGCm'C
Cuts at: 0 256 838 948
Size: 256 582 110
Fragments arranged by size:
582 256 110
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 757 909 948
Size: 757 152 39
Fragments arranged by size:
757 152 39
Bpu10I CC'TnA_GC
Cuts at: 0 670 948
Size: 670 278
BsaI GGTCTCn'nnnn_
Cuts at: 0 823 948
Size: 823 125
BsaAI yAC'GTr
Cuts at: 0 363 948
Size: 363 585
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 28 948
Size: 28 920
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 708 948
Size: 708 240
BseYI C'CCAG_C
Cuts at: 0 54 948
Size: 54 894
BsiHKAI G_wGCw'C
Cuts at: 0 894 948
Size: 894 54
Bsp1286I G_dGCh'C
Cuts at: 0 256 838 894 948
Size: 256 582 56 54
Fragments arranged by size:
582 256 56 54
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 721 948
Size: 721 227
BsrDI GCAATG_nn'
Cuts at: 0 758 948
Size: 758 190
BstEII G'GTnAC_C
Cuts at: 0 735 948
Size: 735 213
BstZ17I GTA'TAC
Cuts at: 0 861 948
Size: 861 87
Bsu36I CC'TnA_GG
Cuts at: 0 868 948
Size: 868 80
BtgI C'CryG_G
Cuts at: 0 20 948
Size: 20 928
BtsI GCAGTG_nn'
Cuts at: 0 653 671 836 948
Size: 653 18 165 112
Fragments arranged by size:
653 165 112 18
ClaI AT'CG_AT
Cuts at: 0 460 948
Size: 460 488
DraIII CAC_nnn'GTG
Cuts at: 0 326 948
Size: 326 622
EarI CTCTTCn'nnn_
Cuts at: 0 301 748 826 948
Size: 301 447 78 122
Fragments arranged by size:
447 301 122 78
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 757 909 948
Size: 757 152 39
Fragments arranged by size:
757 152 39
EcoO109I rG'GnC_Cy
Cuts at: 0 252 534 710 948
Size: 252 282 176 238
Fragments arranged by size:
282 252 238 176
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 272 304 523 555 948
Size: 272 32 219 32 393
Fragments arranged by size:
393 272 219 32 32
HincII GTy'rAC
Cuts at: 0 6 243 948
Size: 6 237 705
Fragments arranged by size:
705 237 6
HpaI GTT'AAC
Cuts at: 0 6 948
Size: 6 942
MslI CAynn'nnrTG
Cuts at: 0 610 948
Size: 610 338
MspA1I CmG'CkG
Cuts at: 0 423 948
Size: 423 525
NcoI C'CATG_G
Cuts at: 0 20 948
Size: 20 928
PasI CC'CwG_GG
Cuts at: 0 312 948
Size: 312 636
PflMI CCAn_nnn'nTGG
Cuts at: 0 237 692 948
Size: 237 455 256
Fragments arranged by size:
455 256 237
PpuMI rG'GwC_Cy
Cuts at: 0 534 710 948
Size: 534 176 238
Fragments arranged by size:
534 238 176
PspOMI G'GGCC_C
Cuts at: 0 252 948
Size: 252 696
PvuII CAG'CTG
Cuts at: 0 423 948
Size: 423 525
SapI GCTCTTCn'nnn_
Cuts at: 0 301 948
Size: 301 647
SfcI C'TryA_G
Cuts at: 0 747 948
Size: 747 201
StuI AGG'CCT
Cuts at: 0 703 948
Size: 703 245
StyI C'CwwG_G
Cuts at: 0 20 795 948
Size: 20 775 153
Fragments arranged by size:
775 153 20
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 68 371 948
Size: 68 303 577
Fragments arranged by size:
577 303 68
TatI w'GTAC_w
Cuts at: 0 141 170 948
Size: 141 29 778
Fragments arranged by size:
778 141 29
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 113 145 948
Size: 113 32 803
Fragments arranged by size:
803 113 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 273 948
Size: 273 675
Enzymes that do cut:
AccI AlwNI ApaI BanII BbsI BbvCI Bme1580I BpmI
Bpu10I BsaI BsaAI BsaXI BseRI BseYI BsiHKAI Bsp1286I
BspMI BsrDI BstEII BstZ17I Bsu36I BtgI BtsI ClaI
DraIII EarI Eco57MI EcoO109I Hin4I HincII HpaI MslI
MspA1I NcoI PasI PflMI PpuMI PspOMI PvuII SapI
SfcI StuI StyI TaqII TatI TstI XcmI
Enzymes that do not cut:
AarI AatII Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI ApaLI ApoI AscI
AseI AsiSI AvaI AvrII BaeI BamHI BanI BbeI
BcgI BciVI BclI BfrBI BglI BglII BlpI BmgBI
BmrI BmtI BplI BpuEI BsaBI BsaHI BsaWI BsgI
BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstXI BstYI BtgZI
CspCI DraI DrdI EaeI EagI EciI EcoICRI EcoNI
EcoRI EcoRV FalI FseI FspI FspAI HaeII HindIII
KasI KpnI MfeI MluI MmeI MscI NaeI NarI
NdeI NgoMIV NheI NotI NruI NsiI NspI PacI
PciI PfoI PmeI PmlI PpiI PshAI PsiI PspXI
PsrI PstI PvuI RsrII SacI SacII SalI SanDI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SrfI SspI SwaI Tth111I
XbaI XhoI XmaI XmnI ZraI