Restriction map for pF1KB7118
 (Linear) (Six-base) MAPSORT of: KIBB7118.seq  Check: 6397  from: 1  to: 849



KIBB7118.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 10:58  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     109     432     849 
   Size:        109     323     417

  Fragments arranged by size: 

                 417     323     109
 
AhdI GACnn_n'nnGTC

Cuts at:      0     667     849 
   Size:        667     182
 
AleI CACnn'nnGTG

Cuts at:      0     842     849 
   Size:        842       7
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     535     567     849 
   Size:        535      32     282

  Fragments arranged by size: 

                 535     282      32
 
ApaI G_GGCC'C

Cuts at:      0     206     229     439     849 
   Size:        206      23     210     410

  Fragments arranged by size: 

                 410     210     206      23
 
AvaI C'yCGr_G

Cuts at:      0     153     440     484     849 
   Size:        153     287      44     365

  Fragments arranged by size: 

                 365     287     153      44
 
BanII G_rGCy'C

Cuts at:      0     139     206     229     307     439     471     849 
   Size:        139      67      23      78     132      32     378

  Fragments arranged by size: 

                 378     139     132      78      67      32      23
 
BbvCI CC'TCA_GC

Cuts at:      0     184     372     849 
   Size:        184     188     477

  Fragments arranged by size: 

                 477     188     184
 
Bme1580I G_kGCm'C

Cuts at:      0      86     206     229     439     849 
   Size:         86     120      23     210     410

  Fragments arranged by size: 

                 410     210     120      86      23
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     580     667     836     849 
   Size:        580      87     169      13

  Fragments arranged by size: 

                 580     169      87      13
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     723     849 
   Size:        723     126
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     124     499     849 
   Size:        124     375     350

  Fragments arranged by size: 

                 375     350     124
 
Bpu10I CC'TnA_GC

Cuts at:      0     184     372     708     849 
   Size:        184     188     336     141

  Fragments arranged by size: 

                 336     188     184     141
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     532     739     849 
   Size:        532     207     110

  Fragments arranged by size: 

                 532     207     110
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     770     849 
   Size:        770      79
 
BsaAI yAC'GTr

Cuts at:      0     282     411     849 
   Size:        282     129     438

  Fragments arranged by size: 

                 438     282     129
 
BsaBI GATnn'nnATC

Cuts at:      0     328     849 
   Size:        328     521
 
BsaHI Gr'CG_yC

Cuts at:      0      71     849 
   Size:         71     778
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     688     718     849 
   Size:        688      30     131

  Fragments arranged by size: 

                 688     131      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      16     138     637     712     849 
   Size:         16     122     499      75     137

  Fragments arranged by size: 

                 499     137     122      75      16
 
BseYI C'CCAG_C

Cuts at:      0      19     206     849 
   Size:         19     187     643

  Fragments arranged by size: 

                 643     187      19
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     551     849 
   Size:        551     298
 
BsiHKAI G_wGCw'C

Cuts at:      0     709     849 
   Size:        709     140
 
Bsp1286I G_dGCh'C

Cuts at:      0      86     139     206     229     307     439     471     709 
   Size:         86      53      67      23      78     132      32     238

Cuts at:    709     849 
   Size:        140

  Fragments arranged by size: 

                 238     140     132      86      78      67      53      32
                  23
 
BsrBI CCG'CTC

Cuts at:      0      77     849 
   Size:         77     772
 
BsrFI r'CCGG_y

Cuts at:      0     736     849 
   Size:        736     113
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     102     198     219     249     597     664     849 
   Size:        102      96      21      30     348      67     185

  Fragments arranged by size: 

                 348     185     102      96      67      30      21
 
BtgI C'CryG_G

Cuts at:      0     341     849 
   Size:        341     508
 
BtsI GCAGTG_nn'

Cuts at:      0     837     849 
   Size:        837      12
 
ClaI AT'CG_AT

Cuts at:      0     196     849 
   Size:        196     653
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     652     800     849 
   Size:        652     148      49

  Fragments arranged by size: 

                 652     148      49
 
EaeI y'GGCC_r

Cuts at:      0     530     734     849 
   Size:        530     204     115

  Fragments arranged by size: 

                 530     204     115
 
EarI CTCTTCn'nnn_

Cuts at:      0     614     849 
   Size:        614     235
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     262     849 
   Size:        262     587
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     109     124     432     499     849 
   Size:        109      15     308      67     350

  Fragments arranged by size: 

                 350     308     109      67      15
 
EcoO109I rG'GnC_Cy

Cuts at:      0     202     203     225     435     436     456     669     714 
   Size:        202       1      22     210       1      20     213      45

Cuts at:    714     802     849 
   Size:         88      47

  Fragments arranged by size: 

                 213     210     202      88      47      45      22      20
                   1       1
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      61      93     124     156     653     685     849 
   Size:         61      32      31      32     497      32     164

  Fragments arranged by size: 

                 497     164      61      32      32      32      31
 
MslI CAynn'nnrTG

Cuts at:      0     196     842     849 
   Size:        196     646       7

  Fragments arranged by size: 

                 646     196       7
 
MspA1I CmG'CkG

Cuts at:      0     343     849 
   Size:        343     506
 
PasI CC'CwG_GG

Cuts at:      0     219     789     849 
   Size:        219     570      60

  Fragments arranged by size: 

                 570     219      60
 
PfoI T'CCnGG_A

Cuts at:      0     101     248     849 
   Size:        101     147     601

  Fragments arranged by size: 

                 601     147     101
 
PpuMI rG'GwC_Cy

Cuts at:      0     456     669     714     802     849 
   Size:        456     213      45      88      47

  Fragments arranged by size: 

                 456     213      88      47      45
 
PspOMI G'GGCC_C

Cuts at:      0     202     225     435     849 
   Size:        202      23     210     414

  Fragments arranged by size: 

                 414     210     202      23
 
SacII CC_GC'GG

Cuts at:      0     344     849 
   Size:        344     505
 
SanDI GG'GwC_CC

Cuts at:      0     669     802     849 
   Size:        669     133      47

  Fragments arranged by size: 

                 669     133      47
 
SmaI CCC'GGG

Cuts at:      0     442     849 
   Size:        442     407
 
SmlI C'TyrA_G

Cuts at:      0     547     718     849 
   Size:        547     171     131

  Fragments arranged by size: 

                 547     171     131
 
StuI AGG'CCT

Cuts at:      0     370     849 
   Size:        370     479
 
StyI C'CwwG_G

Cuts at:      0     747     849 
   Size:        747     102
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     553     849 
   Size:        553     296
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     688     720     849 
   Size:        688      32     129

  Fragments arranged by size: 

                 688     129      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     246     567     849 
   Size:        246     321     282

  Fragments arranged by size: 

                 321     282     246
 
XmaI C'CCGG_G

Cuts at:      0     440     849 
   Size:        440     409
 

 Enzymes that do cut:

     AcuI     AhdI     AleI     AloI     ApaI     AvaI    BanII    BbvCI
 Bme1580I     BmrI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI
    BsaBI    BsaHI    BsaXI    BseRI    BseYI     BsgI  BsiHKAI Bsp1286I
    BsrBI    BsrFI    BstXI     BtgI     BtsI     ClaI     DrdI     EaeI
     EarI     EciI  Eco57MI EcoO109I    Hin4I     MslI   MspA1I     PasI
     PfoI    PpuMI   PspOMI    SacII    SanDI     SmaI     SmlI     StuI
     StyI    TaqII     TstI     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AlfI    AlwNI    ApaLI     ApoI     AscI     AseI    AsiSI
    AvrII     BaeI    BamHI     BanI     BbeI     BbsI     BcgI    BciVI
     BclI    BfrBI     BglI    BglII     BlpI    BmgBI     BmtI    BsaWI
    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BspMI    BsrDI
    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstYI  BstZ17I
   Bsu36I    BtgZI    CspCI     DraI   DraIII     EagI  EcoICRI    EcoNI
    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII   HincII
  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MscI
     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     NspI     PacI     PciI    PflMI     PmeI     PmlI     PpiI
    PshAI     PsiI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII
     SacI     SalI     SapI     SbfI     ScaI    SexAI     SfcI     SfiI
     SfoI     SgfI    SgrAI    SnaBI     SpeI     SphI     SrfI     SspI
     SwaI     TatI  Tth111I     XbaI     XhoI     XmnI     ZraI
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