Restriction map for pF1KB7116
##################################
# NO CUT ENZYMES (  157 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AflII       AflIII      AgeI        
AhdI        AlwNI       AocI        Aor51HI     ApaI        
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AviII       AvrII       BamHI       BbeI        
BbrPI       BbvCI       BcgI        BclI        BfaI        
BfrI        BglII       BlnI        BlpI        Bpu1102I    
BsaAI       BsaBI       BsaHI       BsaI        BsaWI       
BseAI       BsiEI       BsiWI       BsmI        Bsp1407I    
BspDI       BspEI       BspHI       BspLU11I    BspMI       
BsrBI       BsrDI       BsrFI       BsrGI       BssHII      
BssSI       Bst1107I    BstAPI      BstBI       BstEII      
BstF5I      BstPI       BstUI       BstXI       BstZ17I     
Bsu36I      CelII       Cfr10I      ClaI        CpoI        
DraI        DraII       DraIII      DrdI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      Eco81I      
EcoO109I    EcoO65I     EcoRI       EcoRV       EcoT22I     
FauI        FbaI        FokI        FseI        FspI        
HaeII       HgaI        Hin1I       HindIII     HpaI        
KasI        KpnI        KspI        MaeI        MaeII       
MaeIII      MamI        MfeI        MluI        MroI        
MseI        MspA1I      MunI        MvnI        NaeI        
NarI        NcoI        NdeI        NgoMIV      NheI        
NotI        NruI        NsiI        NspI        NspV        
PacI        PinAI       PmaCI       PmeI        PmlI        
PpuMI       PshAI       PshBI       Psp1406I    PvuI        
PvuII       RcaI        RsrII       SacII       SalI        
SbfI        ScaI        SexAI       SfiI        SfoI        
SfuI        SgrAI       SmaI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        StuI        
SwaI        Tru9I       Tsp45I      XbaI        XmaCI       
XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   46 enzymes) #
###################################

AcsI          517  AAATTC               [GA]^AATT[CT]
Alw44I        248  GTGCAC               G^TGCAC
AlwI          285  GATCC                GGATC(4/5)
ApaLI         248  GTGCAC               G^TGCAC
ApoI          517  AAATTC               [GA]^AATT[CT]
AspI          140  GACCGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaI          494  CTCGAG               C^[CT]CG[GA]G
AvaII         579  GGACC                G^G[AT]CC
BalI            2  TGGCCA               TGG^CCA
BbsI          468  GAAGAC               GAAGAC(2/6)
BcnI          435  CCGGG                CC^[GC]GG
BpuAI         468  GAAGAC               GAAGAC(2/6)
BsgI          780  GTGCAG               GTGCAG(16/14)
BsmAI         454  GAGAC                GTCTC(1/5)
BsmBI         454  GAGACG               CGTCTC(1/5)
BsmFI         129  GGGAC                GGGAC(10/14)
BsoBI         494  CTCGAG               C^[CT]CG[GA]G
BstYI         284  AGATCC               [GA]^GATC[CT]
CfoI          176  GCGC                 GCG^C
DsaI          506  CCGTGG               C^C[GA][CT]GG
EcoNI         372  CCTGGAGGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HhaI          176  GCGC                 GCG^C
HinP1I        176  GCGC                 G^CGC
HincII         64  GTTGAC               GT[CT]^[GA]AC
HindII         64  GTTGAC               GT[CT]^[GA]AC
MflI          284  AGATCC               [GA]^GATC[CT]
MluNI           2  TGGCCA               TGG^CCA
MscI            2  TGGCCA               TGG^CCA
MslI           32  CACCTGAGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NciI          435  CCGGG                CC^[GC]GG
PaeR7I        494  CTCGAG               C^TCGAG
PflFI         140  GACCGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI         159  CCAGGGCGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI          144  GAGTC                GAGTC(4/5)
SacI          491  GAGCTC               GAGCT^C
SapI          545  GCTCTTC              GCTCTTC(1/4)
SfaNI         809  GCATC                GCATC(5/9)
SmlI          494  CTCGAG               C^T[CT][GA]AG
TaqI          495  TCGA                 T^CGA
Tsp509I       518  AATT                 ^AATT
Tth111I       140  GACCGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I       495  TCGA                 T^CGA
Van91I        159  CCAGGGCGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI          668  CCATTGTTGCTGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI          494  CTCGAG               C^TCGAG
XhoII         284  AGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   24 enzymes) #
###################################

AciI          122  CCGC                 CCGC(-3/-1)
              196  CCGC                
AfaI          307  GTAC                 GT^AC
              634  GTAC                
AspHI         248  GTGCAC               G[AT]GC[AT]^C
              491  GAGCTC              
BanI          460  GGCACC               G^G[CT][GA]CC
              735  GGTGCC              
BciVI         339  GTATCC               GTATCC(6/5)
              431  GTATCC              
BglI          400  GCCAAAAAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
              738  GCCCAGGAGGC         
BseRI         376  GAGGAG               GAGGAG(10/8)
              379  GAGGAG              
BsiHKAI       248  GTGCAC               G[AT]GC[AT]^C
              491  GAGCTC              
Cac8I         409  GCCAGC               GC[ACGT]^[ACGT]GC
              600  GCTTGC              
DpnII         285  GATC                 ^GATC
              611  GATC                
EarI          324  CTCTTC               CTCTTC(1/4)
              546  CTCTTC              
EcoT14I       125  CCAAGG               C^C[AT][AT]GG
              589  CCAAGG              
HapII         435  CCGG                 C^CGG
              645  CCGG                
HpaII         435  CCGG                 C^CGG
              645  CCGG                
HphI          613  TCACC                GGTGA(8/7)
              761  TCACC               
Ksp632I       324  CTCTTC               CTCTTC(1/4)
              546  CTCTTC              
MboI          285  GATC                 ^GATC
              611  GATC                
MspI          435  CCGG                 C^CGG
              645  CCGG                
NdeII         285  GATC                 ^GATC
              611  GATC                
NlaIII        105  CATG                 CATG^
              465  CATG                
RsaI          307  GTAC                 GT^AC
              634  GTAC                
Sau3AI        285  GATC                 ^GATC
              611  GATC                
StyI          125  CCAAGG               C^C[AT][AT]GG
              589  CCAAGG              
TfiI          484  GATTC                G^A[AT]TC
              785  GAATC               


#########################################
# 3 OR MORE CUT ENZYMES (   31 enzymes) #
#########################################

AluI        BanII       BbvI        BmyI        BpmI        
BsaJI       BsiYI       BslI        Bsp1286I    BsrI        
BssKI       BstNI       Cfr13I      DdeI        EaeI        
EcoRII      Fnu4HI      HaeIII      HinfI       ItaI        
MboII       MnlI        MvaI        MwoI        NlaIV       
PstI        Sau96I      ScrFI       SfcI        TseI        
TspRI       
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