Restriction map for
pF1KB7116
(Circular) (Six-base) MAPSORT of: pF1KB7116.seq Check: 6756 from: 1 to: 3953
pF1KB7116 3953 bp
With 182 enzymes: SgfI *
June 20, 2008 10:26 ..
AccI GT'mk_AC
Cuts at: 991 991
Size: 3953
Acc65I G'GTAC_C
Cuts at: 969 969
Size: 3953
AclI AA'CG_TT
Cuts at: 3793 3793
Size: 3953
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1778 2210 2957 3225 1778
Size: 432 747 268 2506
Fragments arranged by size:
2506 747 432 268
AflIII A'CryG_T
Cuts at: 1305 2409 3386 1305
Size: 1104 977 1872
Fragments arranged by size:
1872 1104 977
AgeI A'CCGG_T
Cuts at: 2341 2341
Size: 3953
AleI CACnn'nnGTG
Cuts at: 144 144
Size: 3953
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 793 825 793
Size: 32 3921
AlwNI CAG_nnn'CTG
Cuts at: 1258 2825 1258
Size: 1567 2386
ApaLI G'TGCA_C
Cuts at: 356 2723 356
Size: 2367 1586
ApoI r'AATT_y
Cuts at: 625 957 3306 3562 625
Size: 332 2349 256 1016
Fragments arranged by size:
2349 1016 332 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 3953
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 3953
AvaI C'yCGr_G
Cuts at: 602 973 602
Size: 371 3582
BamHI G'GATC_C
Cuts at: 978 978
Size: 3953
BanI G'GyrC_C
Cuts at: 568 843 969 1383 1631 1666 568
Size: 275 126 414 248 35 2855
Fragments arranged by size:
2855 414 275 248 126 35
BanII G_rGCy'C
Cuts at: 549 603 655 967 1997 549
Size: 54 52 312 1030 2505
Fragments arranged by size:
2505 1030 312 54 52
BbeI G_GCGC'C
Cuts at: 1387 1635 1387
Size: 248 3705
BbsI GAAGACnn'nnnn_
Cuts at: 583 583
Size: 3953
BciVI GTATCCnnnnn_n'
Cuts at: 458 550 1851 2612 3953 458
Size: 92 1301 761 1341 458
Fragments arranged by size:
1341 1301 761 458 92
BclI T'GATC_A
Cuts at: 1473 1473
Size: 3953
BglI GCCn_nnn'nGGC
Cuts at: 514 852 514
Size: 338 3615
BglII A'GATC_T
Cuts at: 1468 3505 1468
Size: 2037 1916
BlpI GC'TnA_GC
Cuts at: 1065 1065
Size: 3953
Bme1580I G_kGCm'C
Cuts at: 360 571 848 1578 1671 2727 360
Size: 211 277 730 93 1056 1586
Fragments arranged by size:
1586 1056 730 277 211 93
BmrI ACTGGGnnnn_n'
Cuts at: 431 1165 1246 1581 3725 431
Size: 734 81 335 2144 659
Fragments arranged by size:
2144 734 659 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2320 2320
Size: 3953
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 293 502 694 293
Size: 209 192 3552
Fragments arranged by size:
3552 209 192
Bpu10I CC'TnA_GC
Cuts at: 398 398
Size: 3953
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1129 2008 2500 2798 1129
Size: 879 492 298 2284
Fragments arranged by size:
2284 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3657 3657
Size: 3953
BsaAI yAC'GTr
Cuts at: 1306 1306
Size: 3953
BsaBI GATnn'nnATC
Cuts at: 6 1467 3504 6
Size: 1461 2037 455
Fragments arranged by size:
2037 1461 455
BsaHI Gr'CG_yC
Cuts at: 1384 1632 2334 3830 1384
Size: 248 702 1496 1507
Fragments arranged by size:
1507 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1142 1663 2341 2615 2762 3173 1142
Size: 521 678 274 147 411 1922
Fragments arranged by size:
1922 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 793 823 793
Size: 30 3923
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 499 502 499
Size: 3 3950
BseYI C'CCAG_C
Cuts at: 912 1378 2136 2713 3209 912
Size: 466 758 577 496 1656
Fragments arranged by size:
1656 758 577 496 466
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 909 909
Size: 3953
BsiEI CG_ry'CG
Cuts at: 104 1495 1541 2749 104
Size: 1391 46 1208 1308
Fragments arranged by size:
1391 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 360 603 967 1745 1935 2727 360
Size: 243 364 778 190 792 1586
Fragments arranged by size:
1586 792 778 364 243 190
BsmBI CGTCTCn'nnnn_
Cuts at: 556 556
Size: 3953
Bsp1286I G_dGCh'C
Cuts at: 360 549 571 603 655 848 967 1578 1671
Size: 189 22 32 52 193 119 611 93
Cuts at: 1671 1745 1935 1997 2727 360
Size: 74 190 62 730 1586
Fragments arranged by size:
1586 730 611 193 190 189 119 93
74 62 52 32 22
BspEI T'CCGG_A
Cuts at: 3173 3173
Size: 3953
BspHI T'CATG_A
Cuts at: 3129 3129
Size: 3953
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1003 1217 1519 1900 1003
Size: 214 302 381 3056
Fragments arranged by size:
3056 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2245 2299 3783 3948 50
Size: 2195 54 1484 165 55
Fragments arranged by size:
2195 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1865 1865
Size: 3953
BsrFI r'CCGG_y
Cuts at: 1951 2132 2341 1951
Size: 181 209 3563
Fragments arranged by size:
3563 209 181
BssSI C'ACGA_G
Cuts at: 2224 2582 2224
Size: 358 3595
BstAPI GCAn_nnn'nTGC
Cuts at: 1330 1330
Size: 3953
BstBI TT'CG_AA
Cuts at: 2314 2314
Size: 3953
BstYI r'GATC_y
Cuts at: 1 392 978 1445 1468 1803 2049 3061 3147
Size: 391 586 467 23 335 246 1012 86
Cuts at: 3147 3159 3505 1
Size: 12 346 449
Fragments arranged by size:
1012 586 467 449 391 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 614 107
Size: 507 3446
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1903 2084 2247 1903
Size: 181 163 3609
Fragments arranged by size:
3609 181 163
BtsI GCAGTG_nn'
Cuts at: 523 819 1196 523
Size: 296 377 3280
Fragments arranged by size:
3280 377 296
DraI TTT'AAA
Cuts at: 952 952
Size: 3953
DrdI GACnn_nn'nnGTC
Cuts at: 1659 2517 1659
Size: 858 3095
EaeI y'GGCC_r
Cuts at: 110 754 788 1538 1712 2103 2130 3475 110
Size: 644 34 750 174 391 27 1345 588
Fragments arranged by size:
1345 750 644 588 391 174 34 27
EagI C'GGCC_G
Cuts at: 1538 1538
Size: 3953
EarI CTCTTCn'nnn_
Cuts at: 438 660 1976 2186 438
Size: 222 1316 210 2205
Fragments arranged by size:
2205 1316 222 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 219 293 2161 2471 2617 3763 219
Size: 74 1868 310 146 1146 409
Fragments arranged by size:
1868 1146 409 310 146 74
EcoICRI GAG'CTC
Cuts at: 601 965 601
Size: 364 3589
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 293 502 694 1778 2210 2957 3225 293
Size: 209 192 1084 432 747 268 1021
Fragments arranged by size:
1084 1021 747 432 268 209 192
EcoNI CCTnn'n_nnAGG
Cuts at: 484 484
Size: 3953
EcoO109I rG'GnC_Cy
Cuts at: 1092 1092
Size: 3953
EcoRI G'AATT_C
Cuts at: 957 957
Size: 3953
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1972 1972
Size: 3953
FspI TGC'GCA
Cuts at: 1734 1734
Size: 3953
HaeII r_GCGC'y
Cuts at: 1387 1635 2657 3636 1387
Size: 248 1022 979 1704
Fragments arranged by size:
1704 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 667 699 1795 1825 1827 1857 667
Size: 32 1096 30 2 30 2763
Fragments arranged by size:
2763 1096 32 30 30 2
HincII GTy'rAC
Cuts at: 174 992 174
Size: 818 3135
KasI G'GCGC_C
Cuts at: 1383 1631 1383
Size: 248 3705
KpnI G_GTAC'C
Cuts at: 973 973
Size: 3953
MluI A'CGCG_T
Cuts at: 3386 3386
Size: 3953
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2624 2808 2624
Size: 184 3769
MscI TGG'CCA
Cuts at: 112 1714 112
Size: 1602 2351
MslI CAynn'nnrTG
Cuts at: 144 1942 2069 144
Size: 1798 127 2028
Fragments arranged by size:
2028 1798 127
MspA1I CmG'CkG
Cuts at: 1064 1378 1738 2751 2996 1064
Size: 314 360 1013 245 2021
Fragments arranged by size:
2021 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2134 2134
Size: 3953
NarI GG'CG_CC
Cuts at: 1384 1632 1384
Size: 248 3705
NcoI C'CATG_G
Cuts at: 107 107
Size: 3953
NgoMIV G'CCGG_C
Cuts at: 2132 2132
Size: 3953
NspI r_CATG'y
Cuts at: 1006 1506 2413 1006
Size: 500 907 2546
Fragments arranged by size:
2546 907 500
PasI CC'CwG_GG
Cuts at: 267 267
Size: 3953
PciI A'CATG_T
Cuts at: 2409 2409
Size: 3953
PflMI CCAn_nnn'nTGG
Cuts at: 273 273
Size: 3953
PmeI GTTT'AAAC
Cuts at: 952 952
Size: 3953
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 793 825 3092 3124 793
Size: 32 2267 32 1622
Fragments arranged by size:
2267 1622 32 32
PspXI vC'TCGA_Gb
Cuts at: 602 602
Size: 3953
PstI C_TGCA'G
Cuts at: 260 407 826 1000 1685 260
Size: 147 419 174 685 2528
Fragments arranged by size:
2528 685 419 174 147
PvuI CG_AT'CG
Cuts at: 104 104
Size: 3953
PvuII CAG'CTG
Cuts at: 1378 1738 1378
Size: 360 3593
RsrII CG'GwC_CG
Cuts at: 2148 2148
Size: 3953
SacI G_AGCT'C
Cuts at: 603 967 603
Size: 364 3589
SalI G'TCGA_C
Cuts at: 990 990
Size: 3953
SapI GCTCTTCn'nnn_
Cuts at: 660 1976 2186 660
Size: 1316 210 2427
Fragments arranged by size:
2427 1316 210
SbfI CC_TGCA'GG
Cuts at: 1000 1000
Size: 3953
ScaI AGT'ACT
Cuts at: 3470 3470
Size: 3953
SfcI C'TryA_G
Cuts at: 33 256 403 822 996 1681 2674 2865 33
Size: 223 147 419 174 685 993 191 1121
Fragments arranged by size:
1121 993 685 419 223 191 174 147
SfoI GGC'GCC
Cuts at: 1385 1633 1385
Size: 248 3705
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 3953
SmaI CCC'GGG
Cuts at: 975 975
Size: 3953
SmlI C'TyrA_G
Cuts at: 602 1108 2023 2515 2777 602
Size: 506 915 492 262 1778
Fragments arranged by size:
1778 915 506 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 3953
SphI G_CATG'C
Cuts at: 1006 1506 1006
Size: 500 3453
StyI C'CwwG_G
Cuts at: 107 233 697 1087 1441 107
Size: 126 464 390 354 2619
Fragments arranged by size:
2619 464 390 354 126
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 264 375 1536 1669 2128 2337 264
Size: 111 1161 133 459 209 1880
Fragments arranged by size:
1880 1161 459 209 133 111
TatI w'GTAC_w
Cuts at: 3468 3468
Size: 3953
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3470 3502 3470
Size: 32 3921
Tth111I GACn'n_nGTC
Cuts at: 251 1750 251
Size: 1499 2454
XbaI T'CTAG_A
Cuts at: 984 984
Size: 3953
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 783 1448 783
Size: 665 3288
XhoI C'TCGA_G
Cuts at: 602 602
Size: 3953
XmaI C'CCGG_G
Cuts at: 973 973
Size: 3953
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AloI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PasI PciI PflMI PmeI
PpiI PspXI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI ApaI AscI
AvrII BaeI BbvCI BcgI BfrBI BmgBI BmtI BsiWI
BsmI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I ClaI
CspCI DraIII EcoRV FseI FspAI HindIII HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PfoI PmlI
PpuMI PshAI PsiI PspOMI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI SspI StuI SwaI XmnI
ZraI