Restriction map for pF1KB7111
##################################
# NO CUT ENZYMES (  155 enzymes) #
##################################

AatII       AccI        AccII       AccIII      AciI        
AclI        AcsI        AcyI        AflII       AflIII      
AgeI        AhdI        Alw44I      AlwNI       AocI        
ApaLI       ApoI        AscI        AseI        AsnI        
AspEI       AvaI        AvaII       AviII       AvrII       
BamHI       BbeI        BbrPI       BbvCI       BciVI       
BclI        BfrI        BglI        BglII       BlnI        
BlpI        Bpu1102I    BsaAI       BsaBI       BsaHI       
BsaI        BsaWI       BseAI       BsiEI       BsiWI       
BsmI        BsoBI       Bsp1407I    BspDI       BspEI       
BspLU11I    BsrBI       BsrDI       BsrFI       BsrGI       
BsrI        BssHII      Bst1107I    BstAPI      BstBI       
BstEII      BstPI       BstUI       BstYI       BstZ17I     
Bsu36I      CelII       Cfr10I      ClaI        CpoI        
DdeI        DraI        DraIII      DrdI        DsaI        
EagI        Eam1105I    EclXI       Eco52I      Eco81I      
EcoO65I     EcoRI       EcoRV       EcoT14I     FauI        
FbaI        FseI        FspI        Hin1I       HindIII     
HpaI        KasI        KspI        MamI        MfeI        
MflI        MluI        MroI        MunI        MvnI        
MwoI        NaeI        NarI        NcoI        NdeI        
NgoMIV      NheI        NotI        NruI        NspV        
PacI        PaeR7I      PflMI       PinAI       PmaCI       
PmeI        PmlI        PpuMI       PshAI       PshBI       
Psp1406I    PstI        PvuI        RsrII       SacI        
SacII       SalI        SapI        SbfI        ScaI        
SexAI       SfiI        SfoI        SfuI        SgrAI       
SmaI        SmlI        SnaBI       SpeI        Sse8387I    
SspBI       SspI        StuI        StyI        SwaI        
TaqI        Tsp509I     TthHB8I     Van91I      XbaI        
XcmI        XhoI        XhoII       XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   54 enzymes) #
###################################

Acc65I        479  GGTACC               G^GTACC
Aor51HI       261  AGCGCT               AGC^GCT
ApaI          230  GGGCCC               GGGCC^C
Asp700I       336  GAATGATTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I       479  GGTACC               G^GTACC
AspHI         671  GAGCAC               G[AT]GC[AT]^C
AspI          148  GACTTCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BalI          272  TGGCCA               TGG^CCA
BanI          479  GGTACC               G^G[CT][GA]CC
BanII         230  GGGCCC               G[GA]GC[CT]^C
BbsI          561  GAAGAC               GAAGAC(2/6)
BcgI          369  GCAGAAGGTTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI          548  CTAG                 C^TAG
BpmI          235  CTCCAG               CTGGAG(16/14)
BpuAI         561  GAAGAC               GAAGAC(2/6)
BseRI         323  GAGGAG               GAGGAG(10/8)
BsgI           16  CTGCAC               GTGCAG(16/14)
BsiHKAI       671  GAGCAC               G[AT]GC[AT]^C
BsmAI         250  GTCTC                GTCTC(1/5)
BsmBI         249  CGTCTC               CGTCTC(1/5)
BspHI          98  TCATGA               T^CATGA
BssSI         106  CTCGTG               CACGAG(-5/-1)
BstXI         275  CCATGCATGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cac8I         605  GCATGC               GC[ACGT]^[ACGT]GC
CfoI          262  GCGC                 GCG^C
DraII         230  GGGCCCT              [GA]G^G[ACGT]CC[CT]
EaeI          272  TGGCCA               [CT]^GGCC[GA]
EarI          330  GAAGAG               CTCTTC(1/4)
Eco47III      261  AGCGCT               AGC^GCT
EcoNI         287  CCTACTACAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I      230  GGGCCCT              [GA]G^G[ACGT]CC[CT]
EcoT22I       277  ATGCAT               ATGCA^T
HaeII         261  AGCGCT               [GA]GCGC^[CT]
HhaI          262  GCGC                 GCG^C
HinP1I        262  GCGC                 G^CGC
KpnI          479  GGTACC               GGTAC^C
Ksp632I       330  GAAGAG               CTCTTC(1/4)
MaeI          548  CTAG                 C^TAG
MaeIII        134  GTGAC                ^GT[ACGT]AC
MluNI         272  TGGCCA               TGG^CCA
MscI          272  TGGCCA               TGG^CCA
MspA1I        238  CAGCTG               C[AC]G^C[GT]G
NsiI          277  ATGCAT               ATGCA^T
PflFI         148  GACTTCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PleI           95  GAGTC                GAGTC(4/5)
PvuII         238  CAGCTG               CAG^CTG
RcaI           98  TCATGA               T^CATGA
SfcI          291  CTACAG               C^T[GA][CT]AG
SphI          605  GCATGC               GCATG^C
TfiI          428  GAATC                G^A[AT]TC
Tsp45I        134  GTGAC                ^GT[GC]AC
TspRI         164  CACTG                CA[GC]TG[ACGT][ACGT]^
Tth111I       148  GACTTCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XmnI          336  GAATGATTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   27 enzymes) #
###################################

AfaI          405  GTAC                 GT^AC
              480  GTAC                
AluI          239  AGCT                 AG^CT
              737  AGCT                
AlwI          102  GATCC                GGATC(4/5)
               71  GGATC               
BcnI          172  CCGGG                CC^[GC]GG
              207  CCCGG               
BmyI          230  GGGCCC               G[AGT]GC[ACT]^C
              671  GAGCAC              
BsmFI          10  GGGAC                GGGAC(10/14)
              213  GTCCC               
Bsp1286I      230  GGGCCC               G[AGT]GC[ACT]^C
              671  GAGCAC              
BspMI         485  ACCTGC               ACCTGC(4/8)
              635  ACCTGC              
BstF5I        219  CATCC                GGATG(2/0)
              175  GGATG               
BstNI         120  CCAGG                CC^[AT]GG
              491  CCTGG               
Cfr13I        230  GGGCC                G^G[ACGT]CC
              231  GGCCC               
EcoRII        120  CCAGG                ^CC[AT]GG
              491  CCTGG               
FokI          219  CATCC                GGATG(9/13)
              175  GGATG               
HincII         37  GTCAAC               GT[CT]^[GA]AC
              532  GTTGAC              
HindII         37  GTCAAC               GT[CT]^[GA]AC
              532  GTTGAC              
HinfI          95  GAGTC                G^A[ACGT]TC
              428  GAATC               
HphI          133  GGTGA                GGTGA(8/7)
              690  TCACC               
MaeII         403  ACGT                 A^CGT
              617  ACGT                
MseI          362  TTAA                 T^TAA
              614  TTAA                
MslI          276  CATGCATGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              576  CATTTTTATG          
MvaI          120  CCAGG                CC^[AT]GG
              491  CCTGG               
NciI          172  CCGGG                CC^[GC]GG
              207  CCCGG               
NlaIV         230  GGGCCC               GG[ACGT]^[ACGT]CC
              479  GGTACC              
NspI          279  GCATGT               [GA]CATG^[CT]
              605  GCATGC              
RsaI          405  GTAC                 GT^AC
              480  GTAC                
Sau96I        230  GGGCC                G^G[ACGT]CC
              231  GGCCC               
Tru9I         362  TTAA                 T^TAA
              614  TTAA                


#########################################
# 3 OR MORE CUT ENZYMES (   22 enzymes) #
#########################################

BbvI        BsaJI       BsiYI       BslI        BssKI       
DpnII       Fnu4HI      HaeIII      HapII       HgaI        
HpaII       ItaI        MboI        MboII       MnlI        
MspI        NdeII       NlaIII      Sau3AI      ScrFI       
SfaNI       TseI        
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com