Restriction map for pF1KB7097
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# NO CUT ENZYMES (  174 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcsI        AcyI        AflII       AgeI        
AhdI        AluI        Alw44I      AlwNI       Aor51HI     
ApaI        ApaLI       ApoI        AscI        AseI        
AsnI        Asp700I     Asp718I     AspEI       AspI        
AvaI        AviII       AvrII       BanII       BbeI        
BbrPI       BbsI        BbvCI       BciVI       BclI        
BcnI        BfaI        BfrI        BglI        BglII       
BlnI        BlpI        BpmI        Bpu1102I    BpuAI       
BsaBI       BsaHI       BsaJI       BsaWI       BseAI       
BsgI        BsiWI       BsmBI       BsmI        BsoBI       
BspDI       BspEI       BsrBI       BsrDI       BsrFI       
BssHII      Bst1107I    BstAPI      BstBI       BstEII      
BstPI       BstUI       BstXI       BstZ17I     CelII       
Cfr10I      ClaI        CpoI        DraI        DraIII      
DrdI        DsaI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      EcoNI       EcoO65I     EcoRI       
EcoRV       EcoT14I     EcoT22I     FbaI        FseI        
FspI        HapII       HgaI        Hin1I       HincII      
HindII      HindIII     HpaI        HpaII       HphI        
KasI        KpnI        KspI        MaeI        MaeIII      
MamI        MfeI        MluI        MroI        MseI        
MspA1I      MspI        MunI        MvnI        MwoI        
NaeI        NarI        NciI        NcoI        NdeI        
NgoMIV      NheI        NotI        NruI        NsiI        
NspV        PacI        PaeR7I      PflFI       PflMI       
PinAI       PmaCI       PmeI        PmlI        PshAI       
PshBI       Psp1406I    PstI        PvuII       RsrII       
SacI        SacII       SalI        SapI        SbfI        
ScaI        SfcI        SfiI        SfoI        SfuI        
SgrAI       SmaI        SmlI        SnaBI       SpeI        
SphI        Sse8387I    SspI        StyI        SwaI        
TaqI        TfiI        Tru9I       Tsp45I      Tsp509I     
Tth111I     TthHB8I     Van91I      XbaI        XcmI        
XhoI        XmaCI       XmaI        XmnI        


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# ONE CUT ENZYMES (   39 enzymes) #
###################################

AflIII         97  ACATGT               A^C[GA][CT]GT
AspHI         557  GAGCAC               G[AT]GC[AT]^C
BamHI         329  GGATCC               G^GATCC
BanI          544  GGTGCC               G^G[CT][GA]CC
BcgI          407  GCAGCGCCATCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaAI          16  TACGTG               [CT]AC^GT[GA]
BsaI          294  GGTCTC               GGTCTC(1/5)
BsiEI           9  CGATCG               CG[GA][CT]^CG
BsiHKAI       557  GAGCAC               G[AT]GC[AT]^C
BsmAI         295  GTCTC                GTCTC(1/5)
Bsp1407I      422  TGTACA               T^GTACA
BspHI          53  TCATGA               T^CATGA
BspLU11I       97  ACATGT               A^CATGT
BspMI         367  ACCTGC               ACCTGC(4/8)
BsrGI         422  TGTACA               T^GTACA
BsrI          333  CCAGT                ACTGG(1/-1)
BssSI         161  CTCGTG               CACGAG(-5/-1)
BstYI         329  GGATCC               [GA]^GATC[CT]
CfoI          410  GCGC                 GCG^C
DraII         464  AGGTCCT              [GA]G^G[ACGT]CC[CT]
EarI          397  CTCTTC               CTCTTC(1/4)
EcoO109I      464  AGGTCCT              [GA]G^G[ACGT]CC[CT]
FauI          440  CCCGC                CCCGC(4/6)
HaeII         409  AGCGCC               [GA]GCGC^[CT]
HhaI          410  GCGC                 GCG^C
HinP1I        410  GCGC                 G^CGC
HinfI         395  GACTC                G^A[ACGT]TC
Ksp632I       397  CTCTTC               CTCTTC(1/4)
MaeII          17  ACGT                 A^CGT
MflI          329  GGATCC               [GA]^GATC[CT]
PleI          395  GACTC                GAGTC(4/5)
PpuMI         464  AGGTCCT              [GA]G^G[AT]CC[CT]
PvuI            9  CGATCG               CGAT^CG
RcaI           53  TCATGA               T^CATGA
SexAI         233  ACCAGGT              A^CC[AT]GGT
SspBI         422  TGTACA               T^GTACA
StuI          356  AGGCCT               AGG^CCT
TspRI         560  CACTG                CA[GC]TG[ACGT][ACGT]^
XhoII         329  GGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   16 enzymes) #
###################################

AfaI           15  GTAC                 GT^AC
              423  GTAC                
AlwI          330  GATCC                GGATC(4/5)
              329  GGATC               
BalI           20  TGGCCA               TGG^CCA
               80  TGGCCA              
BmyI          545  GTGCCC               G[AGT]GC[ACT]^C
              557  GAGCAC              
Bsp1286I      545  GTGCCC               G[AGT]GC[ACT]^C
              557  GAGCAC              
Cac8I         207  GCTGGC               GC[ACGT]^[ACGT]GC
              253  GCCTGC              
DpnII          10  GATC                 ^GATC
              330  GATC                
MboI           10  GATC                 ^GATC
              330  GATC                
MboII         179  TCTTC                GAAGA(8/7)
              398  TCTTC               
MluNI          20  TGGCCA               TGG^CCA
               80  TGGCCA              
MscI           20  TGGCCA               TGG^CCA
               80  TGGCCA              
NdeII          10  GATC                 ^GATC
              330  GATC                
NspI           97  ACATGT               [GA]CATG^[CT]
              257  GCATGT              
RsaI           15  GTAC                 GT^AC
              423  GTAC                
Sau3AI         10  GATC                 ^GATC
              330  GATC                
SfaNI         498  GATGC                GCATC(5/9)
               71  GCATC               


#########################################
# 3 OR MORE CUT ENZYMES (   29 enzymes) #
#########################################

AciI        AocI        AvaII       BbvI        BseRI       
BsiYI       BslI        BsmFI       BssKI       BstF5I      
BstNI       Bsu36I      Cfr13I      DdeI        EaeI        
Eco81I      EcoRII      Fnu4HI      FokI        HaeIII      
ItaI        MnlI        MslI        MvaI        NlaIII      
NlaIV       Sau96I      ScrFI       TseI        
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