Restriction map for pF1KB7097
 (Circular) (Six-base) MAPSORT of: pF1KB7097.seq  Check: 2481  from: 1  to: 3695



pF1KB7097  3695 bp

 With 182 enzymes: SgfI * 

                        June 20, 2008 10:26  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:    484     484 
   Size:       3695
 
AccI GT'mk_AC

Cuts at:    733     733 
   Size:       3695
 
Acc65I G'GTAC_C

Cuts at:    711     711 
   Size:       3695
 
AclI AA'CG_TT

Cuts at:   3535    3535 
   Size:       3695
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1520    1952    2699    2967    1520 
   Size:        432     747     268    2248

  Fragments arranged by size: 

                2248     747     432     268
 
AflIII A'CryG_T

Cuts at:    205    1047    2151    3128     205 
   Size:        842    1104     977     772

  Fragments arranged by size: 

                1104     977     842     772
 
AgeI A'CCGG_T

Cuts at:   2083    2083 
   Size:       3695
 
AlwNI CAG_nnn'CTG

Cuts at:   1000    2567    1000 
   Size:       1567    2128
 
ApaLI G'TGCA_C

Cuts at:   2465    2465 
   Size:       3695
 
ApoI r'AATT_y

Cuts at:    699    3048    3304     699 
   Size:       2349     256    1090

  Fragments arranged by size: 

                2349    1090     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       3695
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3695
 
AvaI C'yCGr_G

Cuts at:    715     715 
   Size:       3695
 
BamHI G'GATC_C

Cuts at:    437     720     437 
   Size:        283    3412
 
BanI G'GyrC_C

Cuts at:    652     711    1125    1373    1408     652 
   Size:         59     414     248      35    2939

  Fragments arranged by size: 

                2939     414     248      59      35
 
BanII G_rGCy'C

Cuts at:    709    1739     709 
   Size:       1030    2665
 
BbeI G_GCGC'C

Cuts at:   1129    1377    1129 
   Size:        248    3447
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:    504     538     504 
   Size:         34    3661
 
BciVI GTATCCnnnnn_n'

Cuts at:   1593    2354    3695    1593 
   Size:        761    1341    1593

  Fragments arranged by size: 

                1593    1341     761
 
BclI T'GATC_A

Cuts at:   1215    1215 
   Size:       3695
 
BglII A'GATC_T

Cuts at:   1210    3247    1210 
   Size:       2037    1658
 
BlpI GC'TnA_GC

Cuts at:    807     807 
   Size:       3695
 
Bme1580I G_kGCm'C

Cuts at:    657    1320    1413    2469     657 
   Size:        663      93    1056    1883

  Fragments arranged by size: 

                1883    1056     663      93
 
BmrI ACTGGGnnnn_n'

Cuts at:    907     988    1323    3467     907 
   Size:         81     335    2144    1135

  Fragments arranged by size: 

                2144    1135     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2062    2062 
   Size:       3695
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    871    1750    2242    2540     871 
   Size:        879     492     298    2026

  Fragments arranged by size: 

                2026     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    408    3399     408 
   Size:       2991     704
 
BsaAI yAC'GTr

Cuts at:    126    1048     126 
   Size:        922    2773
 
BsaBI GATnn'nnATC

Cuts at:      6    1209    3246       6 
   Size:       1203    2037     455

  Fragments arranged by size: 

                2037    1203     455
 
BsaHI Gr'CG_yC

Cuts at:   1126    1374    2076    3572    1126 
   Size:        248     702    1496    1249

  Fragments arranged by size: 

                1496    1249     702     248
 
BsaWI w'CCGG_w

Cuts at:    884    1405    2083    2357    2504    2915     884 
   Size:        521     678     274     147     411    1664

  Fragments arranged by size: 

                1664     678     521     411     274     147
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    471     574     675     471 
   Size:        103     101    3491

  Fragments arranged by size: 

                3491     103     101
 
BseYI C'CCAG_C

Cuts at:   1120    1878    2455    2951    1120 
   Size:        758     577     496    1864

  Fragments arranged by size: 

                1864     758     577     496
 
BsiEI CG_ry'CG

Cuts at:    104     120    1237    1283    2491     104 
   Size:         16    1117      46    1208    1308

  Fragments arranged by size: 

                1308    1208    1117      46      16
 
BsiHKAI G_wGCw'C

Cuts at:    669     709    1487    1677    2469     669 
   Size:         40     778     190     792    1895

  Fragments arranged by size: 

                1895     792     778     190      40
 
Bsp1286I G_dGCh'C

Cuts at:    657     669     709    1320    1413    1487    1677    1739    2469 
   Size:         12      40     611      93      74     190      62     730

Cuts at:   2469     657 
   Size:       1883

  Fragments arranged by size: 

                1883     730     611     190      93      74      62      40
                  12
 
BspEI T'CCGG_A

Cuts at:   2915    2915 
   Size:       3695
 
BspHI T'CATG_A

Cuts at:    161    2871     161 
   Size:       2710     985
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    484     745     959    1261    1642     484 
   Size:        261     214     302     381    2537

  Fragments arranged by size: 

                2537     381     302     261     214
 
BsrBI CCG'CTC

Cuts at:     50    1987    2041    3525    3690      50 
   Size:       1937      54    1484     165      55

  Fragments arranged by size: 

                1937    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   1607    1607 
   Size:       3695
 
BsrFI r'CCGG_y

Cuts at:   1693    1874    2083    1693 
   Size:        181     209    3305

  Fragments arranged by size: 

                3305     209     181
 
BsrGI T'GTAC_A

Cuts at:    530     530 
   Size:       3695
 
BssSI C'ACGA_G

Cuts at:    269    1966    2324     269 
   Size:       1697     358    1640

  Fragments arranged by size: 

                1697    1640     358
 
BstAPI GCAn_nnn'nTGC

Cuts at:   1072    1072 
   Size:       3695
 
BstBI TT'CG_AA

Cuts at:   2056    2056 
   Size:       3695
 
BstYI r'GATC_y

Cuts at:      1     437     720    1187    1210    1545    1791    2803    2889 
   Size:        436     283     467      23     335     246    1012      86

Cuts at:   2889    2901    3247       1 
   Size:         12     346     449

  Fragments arranged by size: 

                1012     467     449     436     346     335     283     246
                  86      23      12
 
Bsu36I CC'TnA_GG

Cuts at:    310     433     557     631     658     310 
   Size:        123     124      74      27    3347

  Fragments arranged by size: 

                3347     124     123      74      27
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   1645    1826    1989    1645 
   Size:        181     163    3351

  Fragments arranged by size: 

                3351     181     163
 
BtsI GCAGTG_nn'

Cuts at:    938     938 
   Size:       3695
 
DraI TTT'AAA

Cuts at:    694     694 
   Size:       3695
 
DrdI GACnn_nn'nnGTC

Cuts at:   1401    2259    1401 
   Size:        858    2837
 
EaeI y'GGCC_r

Cuts at:    128     188     425    1280    1454    1845    1872    3217     128 
   Size:         60     237     855     174     391      27    1345     606

  Fragments arranged by size: 

                1345     855     606     391     237     174      60      27
            
 
EagI C'GGCC_G

Cuts at:   1280    1280 
   Size:       3695
 
EarI CTCTTCn'nnn_

Cuts at:    511    1718    1928     511 
   Size:       1207     210    2278

  Fragments arranged by size: 

                2278    1207     210
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   1903    2213    2359    3505    1903 
   Size:        310     146    1146    2093

  Fragments arranged by size: 

                2093    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:    707     707 
   Size:       3695
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1520    1952    2699    2967    1520 
   Size:        432     747     268    2248

  Fragments arranged by size: 

                2248     747     432     268
 
EcoO109I rG'GnC_Cy

Cuts at:    573     834     573 
   Size:        261    3434
 
EcoRI G'AATT_C

Cuts at:    699     699 
   Size:       3695
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   1714    1714 
   Size:       3695
 
FspI TGC'GCA

Cuts at:   1476    1476 
   Size:       3695
 
HaeII r_GCGC'y

Cuts at:    521    1129    1377    2399    3378     521 
   Size:        608     248    1022     979     838

  Fragments arranged by size: 

                1022     979     838     608     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    140     172    1537    1567    1569    1599     140 
   Size:         32    1365      30       2      30    2236

  Fragments arranged by size: 

                2236    1365      32      30      30       2
 
HincII GTy'rAC

Cuts at:    734     734 
   Size:       3695
 
KasI G'GCGC_C

Cuts at:   1125    1373    1125 
   Size:        248    3447
 
KpnI G_GTAC'C

Cuts at:    715     715 
   Size:       3695
 
MluI A'CGCG_T

Cuts at:   3128    3128 
   Size:       3695
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   2366    2550    2366 
   Size:        184    3511
 
MscI TGG'CCA

Cuts at:    130     190    1456     130 
   Size:         60    1266    2369

  Fragments arranged by size: 

                2369    1266      60
 
MslI CAynn'nnrTG

Cuts at:    160     376     526     532    1684    1811     160 
   Size:        216     150       6    1152     127    2044

  Fragments arranged by size: 

                2044    1152     216     150     127       6
 
MspA1I CmG'CkG

Cuts at:    806    1120    1480    2493    2738     806 
   Size:        314     360    1013     245    1763

  Fragments arranged by size: 

                1763    1013     360     314     245
 
NaeI GCC'GGC

Cuts at:   1876    1876 
   Size:       3695
 
NarI GG'CG_CC

Cuts at:   1126    1374    1126 
   Size:        248    3447
 
NgoMIV G'CCGG_C

Cuts at:   1874    1874 
   Size:       3695
 
NspI r_CATG'y

Cuts at:    209     369     748    1248    2155     209 
   Size:        160     379     500     907    1749

  Fragments arranged by size: 

                1749     907     500     379     160
 
PciI A'CATG_T

Cuts at:    205    2151     205 
   Size:       1946    1749
 
PfoI T'CCnGG_A

Cuts at:    199     199 
   Size:       3695
 
PmeI GTTT'AAAC

Cuts at:    694     694 
   Size:       3695
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2834    2866    2834 
   Size:         32    3663
 
PpuMI rG'GwC_Cy

Cuts at:    573     573 
   Size:       3695
 
PstI C_TGCA'G

Cuts at:    742    1427     742 
   Size:        685    3010
 
PvuI CG_AT'CG

Cuts at:    104     120     104 
   Size:         16    3679
 
PvuII CAG'CTG

Cuts at:   1120    1480    1120 
   Size:        360    3335
 
RsrII CG'GwC_CG

Cuts at:   1890    1890 
   Size:       3695
 
SacI G_AGCT'C

Cuts at:    709     709 
   Size:       3695
 
SalI G'TCGA_C

Cuts at:    732     732 
   Size:       3695
 
SapI GCTCTTCn'nnn_

Cuts at:   1718    1928    1718 
   Size:        210    3485
 
SbfI CC_TGCA'GG

Cuts at:    742     742 
   Size:       3695
 
ScaI AGT'ACT

Cuts at:   3212    3212 
   Size:       3695
 
SexAI A'CCwGG_T

Cuts at:    341     341 
   Size:       3695
 
SfcI C'TryA_G

Cuts at:     33     738    1423    2416    2607      33 
   Size:        705     685     993     191    1121

  Fragments arranged by size: 

                1121     993     705     685     191
 
SfoI GGC'GCC

Cuts at:   1127    1375    1127 
   Size:        248    3447
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3695
 
SmaI CCC'GGG

Cuts at:    717     717 
   Size:       3695
 
SmlI C'TyrA_G

Cuts at:    850    1765    2257    2519     850 
   Size:        915     492     262    2026

  Fragments arranged by size: 

                2026     915     492     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       3695
 
SphI G_CATG'C

Cuts at:    748    1248     748 
   Size:        500    3195
 
StuI AGG'CCT

Cuts at:    466     466 
   Size:       3695
 
StyI C'CwwG_G

Cuts at:    829    1183     829 
   Size:        354    3341
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   1278    1411    1870    2079    1278 
   Size:        133     459     209    2894

  Fragments arranged by size: 

                2894     459     209     133
 
TatI w'GTAC_w

Cuts at:    530    3210     530 
   Size:       2680    1015
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   3212    3244    3212 
   Size:         32    3663
 
Tth111I GACn'n_nGTC

Cuts at:   1492    1492 
   Size:       3695
 
XbaI T'CTAG_A

Cuts at:    726     726 
   Size:       3695
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   1190    1190 
   Size:       3695
 
XmaI C'CCGG_G

Cuts at:    715     715 
   Size:       3695
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AclI     AcuI   AflIII     AgeI    AlwNI
    ApaLI     ApoI     AseI    AsiSI     AvaI    BamHI     BanI    BanII
     BbeI     BcgI    BciVI     BclI    BglII     BlpI Bme1580I     BmrI
     BplI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BseRI
    BseYI    BsiEI  BsiHKAI Bsp1286I    BspEI    BspHI    BspMI    BsrBI
    BsrDI    BsrFI    BsrGI    BssSI   BstAPI    BstBI    BstYI   Bsu36I
    BtgZI     BtsI     DraI     DrdI     EaeI     EagI     EarI     EciI
  EcoICRI  Eco57MI EcoO109I    EcoRI     FalI     FspI    HaeII    Hin4I
   HincII     KasI     KpnI     MluI     MmeI     MscI     MslI   MspA1I
     NaeI     NarI   NgoMIV     NspI     PciI     PfoI     PmeI     PpiI
    PpuMI     PstI     PvuI    PvuII    RsrII     SacI     SalI     SapI
     SbfI     ScaI    SexAI     SfcI     SfoI     SgfI     SmaI     SmlI
     SpeI     SphI     StuI     StyI    TaqII     TatI     TstI  Tth111I
     XbaI     XcmI     XmaI

 Enzymes that do not cut: 

    AatII     AfeI    AflII     AhdI     AleI     AlfI     AloI     ApaI
     AscI    AvrII     BaeI     BbsI    BbvCI    BfrBI     BglI    BmgBI
     BmtI     BpmI   Bpu10I    BsaXI     BsgI    BsiWI     BsmI    BsmBI
   BssHII   BstEII    BstXI  BstZ17I     BtgI     ClaI    CspCI   DraIII
    EcoNI    EcoRV     FseI    FspAI  HindIII     HpaI     MfeI     NcoI
     NdeI     NheI     NotI     NruI     NsiI     PacI     PasI    PflMI
     PmlI    PshAI     PsiI   PspOMI    PspXI     PsrI    SacII    SanDI
     SfiI    SgrAI    SnaBI     SrfI     SspI     SwaI     XhoI     XmnI
     ZraI
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