Restriction map for pF1KB7090
 (Linear) (Six-base) MAPSORT of: KIBB7090.seq  Check: 9794  from: 1  to: 510



KIBB7090.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 10:58  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     279     510 
   Size:        279     231
 
ApaLI G'TGCA_C

Cuts at:      0     400     510 
   Size:        400     110
 
ApoI r'AATT_y

Cuts at:      0     504     510 
   Size:        504       6
 
BanI G'GyrC_C

Cuts at:      0      37     291     327     510 
   Size:         37     254      36     183

  Fragments arranged by size: 

                 254     183      37      36
 
BanII G_rGCy'C

Cuts at:      0     379     510 
   Size:        379     131
 
BbeI G_GCGC'C

Cuts at:      0     331     510 
   Size:        331     179
 
Bme1580I G_kGCm'C

Cuts at:      0     296     404     510 
   Size:        296     108     106

  Fragments arranged by size: 

                 296     108     106
 
BmgBI CAC'GTC

Cuts at:      0     399     510 
   Size:        399     111
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     405     465     510 
   Size:        405      60      45

  Fragments arranged by size: 

                 405      60      45
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      33      82     460     510 
   Size:         33      49     378      50

  Fragments arranged by size: 

                 378      50      49      33
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     439     510 
   Size:        439      71
 
BsaBI GATnn'nnATC

Cuts at:      0     154     510 
   Size:        154     356
 
BsaHI Gr'CG_yC

Cuts at:      0     328     457     510 
   Size:        328     129      53

  Fragments arranged by size: 

                 328     129      53
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     181     211     510 
   Size:        181      30     299

  Fragments arranged by size: 

                 299     181      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     241     378     433     448     457     510 
   Size:        241     137      55      15       9      53

  Fragments arranged by size: 

                 241     137      55      53      15       9
 
BseYI C'CCAG_C

Cuts at:      0     162     510 
   Size:        162     348
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     307     327     510 
   Size:        307      20     183

  Fragments arranged by size: 

                 307     183      20
 
BsiEI CG_ry'CG

Cuts at:      0      77     510 
   Size:         77     433
 
BsiHKAI G_wGCw'C

Cuts at:      0     379     404     510 
   Size:        379      25     106

  Fragments arranged by size: 

                 379     106      25
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     448     510 
   Size:        448      62
 
Bsp1286I G_dGCh'C

Cuts at:      0     296     379     404     510 
   Size:        296      83      25     106

  Fragments arranged by size: 

                 296     106      83      25
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     279     510 
   Size:        279     231
 
BsrBI CCG'CTC

Cuts at:      0     231     510 
   Size:        231     279
 
BsrDI GCAATG_nn'

Cuts at:      0      99     510 
   Size:         99     411
 
BsrFI r'CCGG_y

Cuts at:      0      76     510 
   Size:         76     434
 
BsrGI T'GTAC_A

Cuts at:      0     215     510 
   Size:        215     295
 
Bsu36I CC'TnA_GG

Cuts at:      0     277     510 
   Size:        277     233
 
BtgI C'CryG_G

Cuts at:      0     263     356     510 
   Size:        263      93     154

  Fragments arranged by size: 

                 263     154      93
 
EaeI y'GGCC_r

Cuts at:      0      74     510 
   Size:         74     436
 
EagI C'GGCC_G

Cuts at:      0      74     510 
   Size:         74     436
 
EcoICRI GAG'CTC

Cuts at:      0     377     510 
   Size:        377     133
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      33      82     460     510 
   Size:         33      49     378      50

  Fragments arranged by size: 

                 378      50      49      33
 
EcoO109I rG'GnC_Cy

Cuts at:      0     482     510 
   Size:        482      28
 
EcoRI G'AATT_C

Cuts at:      0     504     510 
   Size:        504       6
 
HaeII r_GCGC'y

Cuts at:      0     331     510 
   Size:        331     179
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     181     213     510 
   Size:        181      32     297

  Fragments arranged by size: 

                 297     181      32
 
KasI G'GCGC_C

Cuts at:      0     327     510 
   Size:        327     183
 
MspA1I CmG'CkG

Cuts at:      0      89     258     510 
   Size:         89     169     252

  Fragments arranged by size: 

                 252     169      89
 
NarI GG'CG_CC

Cuts at:      0     328     510 
   Size:        328     182
 
NcoI C'CATG_G

Cuts at:      0     356     510 
   Size:        356     154
 
NspI r_CATG'y

Cuts at:      0     312     510 
   Size:        312     198
 
PpuMI rG'GwC_Cy

Cuts at:      0     482     510 
   Size:        482      28
 
PstI C_TGCA'G

Cuts at:      0     317     510 
   Size:        317     193
 
SacI G_AGCT'C

Cuts at:      0     379     510 
   Size:        379     131
 
ScaI AGT'ACT

Cuts at:      0     106     510 
   Size:        106     404
 
SfcI C'TryA_G

Cuts at:      0     313     510 
   Size:        313     197
 
SfoI GGC'GCC

Cuts at:      0     329     510 
   Size:        329     181
 
StyI C'CwwG_G

Cuts at:      0     356     510 
   Size:        356     154
 
TatI w'GTAC_w

Cuts at:      0     104     215     510 
   Size:        104     111     295

  Fragments arranged by size: 

                 295     111     104
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0      57     510 
   Size:         57     453
 

 Enzymes that do cut:

     AarI    ApaLI     ApoI     BanI    BanII     BbeI Bme1580I    BmgBI
     BplI     BpmI     BsaI    BsaBI    BsaHI    BsaXI    BseRI    BseYI
     BsgI    BsiEI  BsiHKAI    BsmBI Bsp1286I    BspMI    BsrBI    BsrDI
    BsrFI    BsrGI   Bsu36I     BtgI     EaeI     EagI  EcoICRI  Eco57MI
 EcoO109I    EcoRI    HaeII    Hin4I     KasI   MspA1I     NarI     NcoI
     NspI    PpuMI     PstI     SacI     ScaI     SfcI     SfoI     StyI
     TatI     XcmI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BbsI    BbvCI
     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI     BmrI
     BmtI   Bpu10I    BpuEI    BsaAI    BsaWI    BsiWI     BsmI    BspEI
    BspHI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI
  BstZ17I    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI
     EarI     EciI    EcoNI    EcoRV     FalI     FseI     FspI    FspAI
   HincII  HindIII     HpaI     KpnI     MfeI     MluI     MmeI     MscI
     MslI     NaeI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI    PvuII    RsrII
    SacII     SalI    SanDI     SapI     SbfI    SexAI     SfiI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     SwaI    TaqII     TstI  Tth111I     XbaI     XhoI     XmaI
     XmnI     ZraI
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