Restriction map for pF1KB7089
 (Circular) (Six-base) MAPSORT of: pF1KB7089.seq  Check: 6407  from: 1  to: 3569



pF1KB7089  3569 bp

 With 182 enzymes: SgfI * 

                        June 20, 2008 10:26  ..

AccI GT'mk_AC

Cuts at:    607     607 
   Size:       3569
 
Acc65I G'GTAC_C

Cuts at:    585     585 
   Size:       3569
 
AclI AA'CG_TT

Cuts at:   3409    3409 
   Size:       3569
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1394    1826    2573    2841    1394 
   Size:        432     747     268    2122

  Fragments arranged by size: 

                2122     747     432     268
 
AflIII A'CryG_T

Cuts at:    308     921    2025    3002     308 
   Size:        613    1104     977     875

  Fragments arranged by size: 

                1104     977     875     613
 
AgeI A'CCGG_T

Cuts at:   1957    1957 
   Size:       3569
 
AlwNI CAG_nnn'CTG

Cuts at:    874    2441     874 
   Size:       1567    2002
 
ApaLI G'TGCA_C

Cuts at:   2339    2339 
   Size:       3569
 
ApoI r'AATT_y

Cuts at:    573    2922    3178     573 
   Size:       2349     256     964

  Fragments arranged by size: 

                2349     964     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       3569
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3569
 
AvaI C'yCGr_G

Cuts at:    589     589 
   Size:       3569
 
BamHI G'GATC_C

Cuts at:    594     594 
   Size:       3569
 
BanI G'GyrC_C

Cuts at:    585     999    1247    1282     585 
   Size:        414     248      35    2872

  Fragments arranged by size: 

                2872     414     248      35
 
BanII G_rGCy'C

Cuts at:    518     527     583    1613     518 
   Size:          9      56    1030    2474

  Fragments arranged by size: 

                2474    1030      56       9
 
BbeI G_GCGC'C

Cuts at:   1003    1251    1003 
   Size:        248    3321
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:    418     452     418 
   Size:         34    3535
 
BciVI GTATCCnnnnn_n'

Cuts at:   1467    2228    3569    1467 
   Size:        761    1341    1467

  Fragments arranged by size: 

                1467    1341     761
 
BclI T'GATC_A

Cuts at:   1089    1089 
   Size:       3569
 
BglI GCCn_nnn'nGGC

Cuts at:    531     531 
   Size:       3569
 
BglII A'GATC_T

Cuts at:   1084    3121    1084 
   Size:       2037    1532
 
BlpI GC'TnA_GC

Cuts at:    681     681 
   Size:       3569
 
Bme1580I G_kGCm'C

Cuts at:   1194    1287    2343    1194 
   Size:         93    1056    2420

  Fragments arranged by size: 

                2420    1056      93
 
BmrI ACTGGGnnnn_n'

Cuts at:    781     862    1197    3341     781 
   Size:         81     335    2144    1009

  Fragments arranged by size: 

                2144    1009     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   1936    1936 
   Size:       3569
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    513     513 
   Size:       3569
 
Bpu10I CC'TnA_GC

Cuts at:    214     214 
   Size:       3569
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    259     745    1624    2116    2414     259 
   Size:        486     879     492     298    1414

  Fragments arranged by size: 

                1414     879     492     486     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   3273    3273 
   Size:       3569
 
BsaAI yAC'GTr

Cuts at:    309     922     309 
   Size:        613    2956
 
BsaBI GATnn'nnATC

Cuts at:      6    1083    3120       6 
   Size:       1077    2037     455

  Fragments arranged by size: 

                2037    1077     455
 
BsaHI Gr'CG_yC

Cuts at:    482    1000    1248    1950    3446     482 
   Size:        518     248     702    1496     605

  Fragments arranged by size: 

                1496     702     605     518     248
 
BsaWI w'CCGG_w

Cuts at:    758    1279    1957    2231    2378    2789     758 
   Size:        521     678     274     147     411    1538

  Fragments arranged by size: 

                1538     678     521     411     274     147
 
BseYI C'CCAG_C

Cuts at:    994    1752    2329    2825     994 
   Size:        758     577     496    1738

  Fragments arranged by size: 

                1738     758     577     496
 
BsiEI CG_ry'CG

Cuts at:    104     210     500    1111    1157    2365     104 
   Size:        106     290     611      46    1208    1308

  Fragments arranged by size: 

                1308    1208     611     290     106      46
 
BsiHKAI G_wGCw'C

Cuts at:    200     583    1361    1551    2343     200 
   Size:        383     778     190     792    1426

  Fragments arranged by size: 

                1426     792     778     383     190
 
Bsp1286I G_dGCh'C

Cuts at:    200     518     527     583    1194    1287    1361    1551    1613 
   Size:        318       9      56     611      93      74     190      62

Cuts at:   1613    2343     200 
   Size:        730    1426

  Fragments arranged by size: 

                1426     730     611     318     190      93      74      62
                  56       9
 
BspEI T'CCGG_A

Cuts at:   2789    2789 
   Size:       3569
 
BspHI T'CATG_A

Cuts at:   2745    2745 
   Size:       3569
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    619     833    1135    1516     619 
   Size:        214     302     381    2672

  Fragments arranged by size: 

                2672     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50    1861    1915    3399    3564      50 
   Size:       1811      54    1484     165      55

  Fragments arranged by size: 

                1811    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   1481    1481 
   Size:       3569
 
BsrFI r'CCGG_y

Cuts at:   1567    1748    1957    1567 
   Size:        181     209    3179

  Fragments arranged by size: 

                3179     209     181
 
BssHII G'CGCG_C

Cuts at:    239     241     239 
   Size:          2    3567
 
BssSI C'ACGA_G

Cuts at:   1840    2198    1840 
   Size:        358    3211
 
BstAPI GCAn_nnn'nTGC

Cuts at:    946     946 
   Size:       3569
 
BstBI TT'CG_AA

Cuts at:   1930    1930 
   Size:       3569
 
BstXI CCAn_nnnn'nTGG

Cuts at:    393     393 
   Size:       3569
 
BstYI r'GATC_y

Cuts at:      1     594    1061    1084    1419    1665    2677    2763    2775 
   Size:        593     467      23     335     246    1012      86      12

Cuts at:   2775    3121       1 
   Size:        346     449

  Fragments arranged by size: 

                1012     593     467     449     346     335     246      86
                  23      12
 
BtgI C'CryG_G

Cuts at:    485     494     485 
   Size:          9    3560
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    372    1519    1700    1863     372 
   Size:       1147     181     163    2078

  Fragments arranged by size: 

                2078    1147     181     163
 
BtsI GCAGTG_nn'

Cuts at:    812     812 
   Size:       3569
 
ClaI AT'CG_AT

Cuts at:    404     404 
   Size:       3569
 
DraI TTT'AAA

Cuts at:    568     568 
   Size:       3569
 
DrdI GACnn_nn'nnGTC

Cuts at:   1275    2133    1275 
   Size:        858    2711
 
EaeI y'GGCC_r

Cuts at:    383     497    1154    1328    1719    1746    3091     383 
   Size:        114     657     174     391      27    1345     861

  Fragments arranged by size: 

                1345     861     657     391     174     114      27
 
EagI C'GGCC_G

Cuts at:    497    1154     497 
   Size:        657    2912
 
EarI CTCTTCn'nnn_

Cuts at:    172    1592    1802     172 
   Size:       1420     210    1939

  Fragments arranged by size: 

                1939    1420     210
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   1777    2087    2233    3379    1777 
   Size:        310     146    1146    1967

  Fragments arranged by size: 

                1967    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:    581     581 
   Size:       3569
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    513    1394    1826    2573    2841     513 
   Size:        881     432     747     268    1241

  Fragments arranged by size: 

                1241     881     747     432     268
 
EcoO109I rG'GnC_Cy

Cuts at:    708     708 
   Size:       3569
 
EcoRI G'AATT_C

Cuts at:    573     573 
   Size:       3569
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   1588    1588 
   Size:       3569
 
FspI TGC'GCA

Cuts at:   1350    1350 
   Size:       3569
 
HaeII r_GCGC'y

Cuts at:    400    1003    1251    2273    3252     400 
   Size:        603     248    1022     979     717

  Fragments arranged by size: 

                1022     979     717     603     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1411    1441    1443    1473    1411 
   Size:         30       2      30    3507

  Fragments arranged by size: 

                3507      30      30       2
 
HincII GTy'rAC

Cuts at:    608     608 
   Size:       3569
 
KasI G'GCGC_C

Cuts at:    999    1247     999 
   Size:        248    3321
 
KpnI G_GTAC'C

Cuts at:    589     589 
   Size:       3569
 
MluI A'CGCG_T

Cuts at:   3002    3002 
   Size:       3569
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:    125     230    2240    2424     125 
   Size:        105    2010     184    1270

  Fragments arranged by size: 

                2010    1270     184     105
 
MscI TGG'CCA

Cuts at:   1330    1330 
   Size:       3569
 
MslI CAynn'nnrTG

Cuts at:   1558    1685    1558 
   Size:        127    3442
 
MspA1I CmG'CkG

Cuts at:    356     496     680     994    1354    2367    2612     356 
   Size:        140     184     314     360    1013     245    1313

  Fragments arranged by size: 

                1313    1013     360     314     245     184     140
 
NaeI GCC'GGC

Cuts at:   1750    1750 
   Size:       3569
 
NarI GG'CG_CC

Cuts at:   1000    1248    1000 
   Size:        248    3321
 
NgoMIV G'CCGG_C

Cuts at:   1748    1748 
   Size:       3569
 
NotI GC'GGCC_GC

Cuts at:    497     497 
   Size:       3569
 
NspI r_CATG'y

Cuts at:    622    1122    2029     622 
   Size:        500     907    2162

  Fragments arranged by size: 

                2162     907     500
 
PciI A'CATG_T

Cuts at:   2025    2025 
   Size:       3569
 
PmeI GTTT'AAAC

Cuts at:    568     568 
   Size:       3569
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2708    2740    2708 
   Size:         32    3537
 
PstI C_TGCA'G

Cuts at:    431     616    1301     431 
   Size:        185     685    2699

  Fragments arranged by size: 

                2699     685     185
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       3569
 
PvuII CAG'CTG

Cuts at:    994    1354     994 
   Size:        360    3209
 
RsrII CG'GwC_CG

Cuts at:   1764    1764 
   Size:       3569
 
SacI G_AGCT'C

Cuts at:    583     583 
   Size:       3569
 
SacII CC_GC'GG

Cuts at:    497     497 
   Size:       3569
 
SalI G'TCGA_C

Cuts at:    606     606 
   Size:       3569
 
SapI GCTCTTCn'nnn_

Cuts at:   1592    1802    1592 
   Size:        210    3359
 
SbfI CC_TGCA'GG

Cuts at:    431     616     431 
   Size:        185    3384
 
ScaI AGT'ACT

Cuts at:   3086    3086 
   Size:       3569
 
SfcI C'TryA_G

Cuts at:     33     427     612    1297    2290    2481      33 
   Size:        394     185     685     993     191    1121

  Fragments arranged by size: 

                1121     993     685     394     191     185
 
SfoI GGC'GCC

Cuts at:   1001    1249    1001 
   Size:        248    3321
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3569
 
SmaI CCC'GGG

Cuts at:    591     591 
   Size:       3569
 
SmlI C'TyrA_G

Cuts at:    274     724    1639    2131    2393     274 
   Size:        450     915     492     262    1450

  Fragments arranged by size: 

                1450     915     492     450     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       3569
 
SphI G_CATG'C

Cuts at:    622    1122     622 
   Size:        500    3069
 
StyI C'CwwG_G

Cuts at:    248     703    1057     248 
   Size:        455     354    2760

  Fragments arranged by size: 

                2760     455     354
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   1152    1285    1744    1953    1152 
   Size:        133     459     209    2768

  Fragments arranged by size: 

                2768     459     209     133
 
TatI w'GTAC_w

Cuts at:    311    3084     311 
   Size:       2773     796
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   3086    3118    3086 
   Size:         32    3537
 
Tth111I GACn'n_nGTC

Cuts at:   1366    1366 
   Size:       3569
 
XbaI T'CTAG_A

Cuts at:    600     600 
   Size:       3569
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:    276    1064     276 
   Size:        788    2781
 
XmaI C'CCGG_G

Cuts at:    589     589 
   Size:       3569
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI   AflIII     AgeI    AlwNI    ApaLI
     ApoI     AseI    AsiSI     AvaI    BamHI     BanI    BanII     BbeI
     BcgI    BciVI     BclI     BglI    BglII     BlpI Bme1580I     BmrI
     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BseYI    BsiEI  BsiHKAI Bsp1286I    BspEI    BspHI    BspMI
    BsrBI    BsrDI    BsrFI   BssHII    BssSI   BstAPI    BstBI    BstXI
    BstYI     BtgI    BtgZI     BtsI     ClaI     DraI     DrdI     EaeI
     EagI     EarI     EciI  EcoICRI  Eco57MI EcoO109I    EcoRI     FalI
     FspI    HaeII    Hin4I   HincII     KasI     KpnI     MluI     MmeI
     MscI     MslI   MspA1I     NaeI     NarI   NgoMIV     NotI     NspI
     PciI     PmeI     PpiI     PstI     PvuI    PvuII    RsrII     SacI
    SacII     SalI     SapI     SbfI     ScaI     SfcI     SfoI     SgfI
     SmaI     SmlI     SpeI     SphI     StyI    TaqII     TatI     TstI
  Tth111I     XbaI     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AfeI    AflII     AhdI     AleI     AlfI     AloI
     ApaI     AscI    AvrII     BaeI     BbsI    BbvCI    BfrBI    BmgBI
     BmtI    BsaXI    BseRI     BsgI    BsiWI     BsmI    BsmBI    BsrGI
   BstEII  BstZ17I   Bsu36I    CspCI   DraIII    EcoNI    EcoRV     FseI
    FspAI  HindIII     HpaI     MfeI     NcoI     NdeI     NheI     NruI
     NsiI     PacI     PasI    PflMI     PfoI     PmlI    PpuMI    PshAI
     PsiI   PspOMI    PspXI     PsrI    SanDI    SexAI     SfiI    SgrAI
    SnaBI     SrfI     SspI     StuI     SwaI     XhoI     XmnI     ZraI
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