Restriction map for pF1KB7087
##################################
# NO CUT ENZYMES (  165 enzymes) #
##################################

AatII       AccI        AccIII      AclI        AcsI        
AcyI        AflII       AflIII      AgeI        AhdI        
Alw44I      AlwNI       AocI        Aor51HI     ApaI        
ApaLI       ApoI        AscI        AseI        AsnI        
Asp700I     AspEI       AspI        AvaI        AvaII       
AviII       AvrII       BalI        BamHI       BbeI        
BbrPI       BbvCI       BcgI        BciVI       BcnI        
BfrI        BglII       BlnI        BlpI        Bpu1102I    
BsaAI       BsaBI       BsaHI       BsaI        BsaWI       
BseAI       BseRI       BsgI        BsiEI       BsiWI       
BsmI        BsoBI       Bsp1407I    BspDI       BspEI       
BspHI       BspLU11I    BspMI       BsrBI       BsrDI       
BsrGI       BssHII      Bst1107I    BstAPI      BstBI       
BstXI       BstZ17I     Bsu36I      CelII       CfoI        
Cfr13I      ClaI        CpoI        DdeI        DraI        
DraII       DraIII      EaeI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      Eco81I      EcoNI       
EcoO109I    EcoRI       EcoRV       EcoT22I     FauI        
FseI        FspI        HaeII       HgaI        HhaI        
Hin1I       HinP1I      HincII      HindII      HpaI        
KasI        MaeII       MamI        MfeI        MluI        
MluNI       MroI        MscI        MseI        MunI        
NarI        NciI        NdeI        NheI        NotI        
NruI        NsiI        NspI        NspV        PacI        
PaeR7I      PflFI       PflMI       PinAI       PmaCI       
PmeI        PmlI        PpuMI       PshAI       PshBI       
Psp1406I    PstI        PvuI        PvuII       RcaI        
RsrII       SalI        Sau96I      SbfI        ScaI        
SexAI       SfiI        SfoI        SfuI        SgrAI       
SmaI        SmlI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        StuI        SwaI        
TaqI        Tru9I       Tth111I     TthHB8I     Van91I      
XbaI        XhoI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   56 enzymes) #
###################################

Acc65I        142  GGTACC               G^GTACC
AccII         216  CGCG                 CG^CG
AfaI          143  GTAC                 GT^AC
AlwI           24  GATCC                GGATC(4/5)
Asp718I       142  GGTACC               G^GTACC
AspHI         148  GAGCTC               G[AT]GC[AT]^C
BanI          142  GGTACC               G^G[CT][GA]CC
BbsI          190  GTCTTC               GAAGAC(2/6)
BclI           89  TGATCA               T^GATCA
BfaI          298  CTAG                 C^TAG
BglI           34  GCCCCAAAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI          303  CTCCAG               CTGGAG(16/14)
BpuAI         190  GTCTTC               GAAGAC(2/6)
BsiHKAI       148  GAGCTC               G[AT]GC[AT]^C
BsmBI         351  GAGACG               CGTCTC(1/5)
BsmFI         100  GTCCC                GGGAC(10/14)
BsrFI         199  GCCGGC               [GA]^CCGG[CT]
BsrI          305  CCAGT                ACTGG(1/-1)
BssSI         373  CTCGTG               CACGAG(-5/-1)
BstEII        210  GGTCACC              G^GT[ACGT]ACC
BstF5I        171  CATCC                GGATG(2/0)
BstPI         210  GGTCACC              G^GT[ACGT]ACC
BstUI         216  CGCG                 CG^CG
BstYI          23  AGATCC               [GA]^GATC[CT]
Cac8I         199  GCCGGC               GC[ACGT]^[ACGT]GC
Cfr10I        199  GCCGGC               [GA]^CCGG[CT]
DrdI          353  GACGGGAGAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I       210  GGTCACC              G^GT[ACGT]ACC
EcoT14I       240  CCATGG               C^C[AT][AT]GG
FbaI           89  TGATCA               T^GATCA
FokI          171  CATCC                GGATG(9/13)
HaeIII        265  GGCC                 GG^CC
HapII         200  CCGG                 C^CGG
HindIII       390  AAGCTT               A^AGCTT
HpaII         200  CCGG                 C^CGG
KpnI          142  GGTACC               GGTAC^C
KspI          215  CCGCGG               CCGC^GG
MaeI          298  CTAG                 C^TAG
MaeIII        211  GTCAC                ^GT[ACGT]AC
MflI           23  AGATCC               [GA]^GATC[CT]
MslI          213  CACCGCGGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI          200  CCGG                 C^CGG
MvnI          216  CGCG                 CG^CG
NaeI          199  GCCGGC               GCC^GGC
NcoI          240  CCATGG               C^CATGG
NgoMIV        199  GCCGGC               G^CCGGC
PleI          360  GAGTC                GAGTC(4/5)
RsaI          143  GTAC                 GT^AC
SacI          148  GAGCTC               GAGCT^C
SacII         215  CCGCGG               CCGC^GG
SfcI          134  CTACAG               C^T[GA][CT]AG
StyI          240  CCATGG               C^C[AT][AT]GG
TfiI          117  GATTC                G^A[AT]TC
Tsp45I        211  GTCAC                ^GT[GC]AC
Tsp509I       235  AATT                 ^AATT
XhoII          23  AGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   25 enzymes) #
###################################

BsiYI         311  CCTTGTGCTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              347  CCAAGAGACGG         
BslI          311  CCTTGTGCTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              347  CCAAGAGACGG         
BsmAI         327  GAGAC                GTCTC(1/5)
              351  GAGAC               
BssKI          27  CCTGG                ^CC[ACGT]GG
              267  CCTGG               
BstNI          27  CCTGG                CC^[AT]GG
              267  CCTGG               
DpnII          24  GATC                 ^GATC
               90  GATC                
DsaI          215  CCGCGG               C^C[GA][CT]GG
              240  CCATGG              
EarI            7  GAAGAG               CTCTTC(1/4)
               66  GAAGAG              
EcoRII         27  CCTGG                ^CC[AT]GG
              267  CCTGG               
HinfI         117  GATTC                G^A[ACGT]TC
              360  GAGTC               
HphI           92  TCACC                GGTGA(8/7)
              212  TCACC               
Ksp632I         7  GAAGAG               CTCTTC(1/4)
               66  GAAGAG              
MboI           24  GATC                 ^GATC
               90  GATC                
MnlI          103  CCTC                 CCTC(7/6)
              302  CCTC                
MspA1I        162  CCGCTG               C[AC]G^C[GT]G
              215  CCGCGG              
MvaI           27  CCTGG                CC^[AT]GG
              267  CCTGG               
NdeII          24  GATC                 ^GATC
               90  GATC                
NlaIII         48  CATG                 CATG^
              241  CATG                
NlaIV          31  GGAGCC               GG[ACGT]^[ACGT]CC
              142  GGTACC              
SapI            7  GAAGAGC              GCTCTTC(1/4)
               66  GAAGAGC             
Sau3AI         24  GATC                 ^GATC
               90  GATC                
ScrFI          27  CCTGG                CC^[ACGT]GG
              267  CCTGG               
SfaNI         167  GCATC                GCATC(5/9)
              203  GCATC               
TspRI         111  CAGTG                CA[GC]TG[ACGT][ACGT]^
              306  CAGTG               
XcmI           95  CCACAGTCCCTCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
              257  CCATCTTTGGCCTGG     


#########################################
# 3 OR MORE CUT ENZYMES (   12 enzymes) #
#########################################

AciI        AluI        BanII       BbvI        BmyI        
BsaJI       Bsp1286I    Fnu4HI      ItaI        MboII       
MwoI        TseI        
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