Restriction map for pF1KB7085
 (Circular) (Six-base) MAPSORT of: pF1KB7085.seq  Check: 2668  from: 1  to: 3500



pF1KB7085  3500 bp

 With 182 enzymes: SgfI * 

                        June 20, 2008 10:26  ..

AccI GT'mk_AC

Cuts at:    347     538     347 
   Size:        191    3309
 
Acc65I G'GTAC_C

Cuts at:    516     516 
   Size:       3500
 
AclI AA'CG_TT

Cuts at:   3340    3340 
   Size:       3500
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1325    1757    2504    2772    1325 
   Size:        432     747     268    2053

  Fragments arranged by size: 

                2053     747     432     268
 
AflIII A'CryG_T

Cuts at:    185     852    1956    2933     185 
   Size:        667    1104     977     752

  Fragments arranged by size: 

                1104     977     752     667
 
AgeI A'CCGG_T

Cuts at:   1888    1888 
   Size:       3500
 
AleI CACnn'nnGTG

Cuts at:    130     130 
   Size:       3500
 
AlwNI CAG_nnn'CTG

Cuts at:    805    2372     805 
   Size:       1567    1933
 
ApaLI G'TGCA_C

Cuts at:   2270    2270 
   Size:       3500
 
ApoI r'AATT_y

Cuts at:    504    2853    3109     504 
   Size:       2349     256     895

  Fragments arranged by size: 

                2349     895     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       3500
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3500
 
AvaI C'yCGr_G

Cuts at:    398     520     398 
   Size:        122    3378
 
BamHI G'GATC_C

Cuts at:    525     525 
   Size:       3500
 
BanI G'GyrC_C

Cuts at:    516     930    1178    1213     516 
   Size:        414     248      35    2803

  Fragments arranged by size: 

                2803     414     248      35
 
BanII G_rGCy'C

Cuts at:    514    1544     514 
   Size:       1030    2470
 
BbeI G_GCGC'C

Cuts at:    934    1182     934 
   Size:        248    3252
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:    343     377     343 
   Size:         34    3466
 
BciVI GTATCCnnnnn_n'

Cuts at:   1398    2159    3500    1398 
   Size:        761    1341    1398

  Fragments arranged by size: 

                1398    1341     761
 
BclI T'GATC_A

Cuts at:   1020    1020 
   Size:       3500
 
BglII A'GATC_T

Cuts at:   1015    3052    1015 
   Size:       2037    1463
 
BlpI GC'TnA_GC

Cuts at:    295     612     295 
   Size:        317    3183
 
Bme1580I G_kGCm'C

Cuts at:   1125    1218    2274    1125 
   Size:         93    1056    2351

  Fragments arranged by size: 

                2351    1056      93
 
BmrI ACTGGGnnnn_n'

Cuts at:    268     487     712     793    1128    3272     268 
   Size:        219     225      81     335    2144     496

  Fragments arranged by size: 

                2144     496     335     225     219      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   1867    1867 
   Size:       3500
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    253     340     427     253 
   Size:         87      87    3326

  Fragments arranged by size: 

                3326      87      87
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    676    1555    2047    2345     676 
   Size:        879     492     298    1831

  Fragments arranged by size: 

                1831     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   3204    3204 
   Size:       3500
 
BsaAI yAC'GTr

Cuts at:    853     853 
   Size:       3500
 
BsaBI GATnn'nnATC

Cuts at:      6    1014    3051       6 
   Size:       1008    2037     455

  Fragments arranged by size: 

                2037    1008     455
 
BsaHI Gr'CG_yC

Cuts at:    136     931    1179    1881    3377     136 
   Size:        795     248     702    1496     259

  Fragments arranged by size: 

                1496     795     702     259     248
 
BsaWI w'CCGG_w

Cuts at:    689    1210    1888    2162    2309    2720     689 
   Size:        521     678     274     147     411    1469

  Fragments arranged by size: 

                1469     678     521     411     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:    292     322     292 
   Size:         30    3470
 
BseYI C'CCAG_C

Cuts at:    925    1683    2260    2756     925 
   Size:        758     577     496    1669

  Fragments arranged by size: 

                1669     758     577     496
 
BsiEI CG_ry'CG

Cuts at:    104    1042    1088    2296     104 
   Size:        938      46    1208    1308

  Fragments arranged by size: 

                1308    1208     938      46
 
BsiHKAI G_wGCw'C

Cuts at:    326     514    1292    1482    2274     326 
   Size:        188     778     190     792    1552

  Fragments arranged by size: 

                1552     792     778     190     188
 
BsmBI CGTCTCn'nnnn_

Cuts at:    472     472 
   Size:       3500
 
Bsp1286I G_dGCh'C

Cuts at:    326     514    1125    1218    1292    1482    1544    2274     326 
   Size:        188     611      93      74     190      62     730    1552

  Fragments arranged by size: 

                1552     730     611     190     188      93      74      62
            
 
BspEI T'CCGG_A

Cuts at:   2720    2720 
   Size:       3500
 
BspHI T'CATG_A

Cuts at:   2676    2676 
   Size:       3500
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    194     550     764    1066    1447     194 
   Size:        356     214     302     381    2247

  Fragments arranged by size: 

                2247     381     356     302     214
 
BsrBI CCG'CTC

Cuts at:     50     308    1792    1846    3330    3495      50 
   Size:        258    1484      54    1484     165      55

  Fragments arranged by size: 

                1484    1484     258     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   1412    1412 
   Size:       3500
 
BsrFI r'CCGG_y

Cuts at:   1498    1679    1888    1498 
   Size:        181     209    3110

  Fragments arranged by size: 

                3110     209     181
 
BssSI C'ACGA_G

Cuts at:   1771    2129    1771 
   Size:        358    3142
 
BstAPI GCAn_nnn'nTGC

Cuts at:    877     877 
   Size:       3500
 
BstBI TT'CG_AA

Cuts at:   1861    1861 
   Size:       3500
 
BstXI CCAn_nnnn'nTGG

Cuts at:    318     318 
   Size:       3500
 
BstYI r'GATC_y

Cuts at:      1     525     992    1015    1350    1596    2608    2694    2706 
   Size:        524     467      23     335     246    1012      86      12

Cuts at:   2706    3052       1 
   Size:        346     449

  Fragments arranged by size: 

                1012     524     467     449     346     335     246      86
                  23      12
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   1450    1631    1794    1450 
   Size:        181     163    3156

  Fragments arranged by size: 

                3156     181     163
 
BtsI GCAGTG_nn'

Cuts at:    743     743 
   Size:       3500
 
DraI TTT'AAA

Cuts at:    499     499 
   Size:       3500
 
DrdI GACnn_nn'nnGTC

Cuts at:   1206    2064    1206 
   Size:        858    2642
 
EaeI y'GGCC_r

Cuts at:    446    1085    1259    1650    1677    3022     446 
   Size:        639     174     391      27    1345     924

  Fragments arranged by size: 

                1345     924     639     391     174      27
 
EagI C'GGCC_G

Cuts at:   1085    1085 
   Size:       3500
 
EarI CTCTTCn'nnn_

Cuts at:   1523    1733    1523 
   Size:        210    3290
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   1708    2018    2164    3310    1708 
   Size:        310     146    1146    1898

  Fragments arranged by size: 

                1898    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:    512     512 
   Size:       3500
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    253     340     427    1325    1757    2504    2772     253 
   Size:         87      87     898     432     747     268     981

  Fragments arranged by size: 

                 981     898     747     432     268      87      87
 
EcoO109I rG'GnC_Cy

Cuts at:    206     237     402     639     206 
   Size:         31     165     237    3067

  Fragments arranged by size: 

                3067     237     165      31
 
EcoRI G'AATT_C

Cuts at:    504     504 
   Size:       3500
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   1519    1519 
   Size:       3500
 
FspI TGC'GCA

Cuts at:   1281    1281 
   Size:       3500
 
HaeII r_GCGC'y

Cuts at:    934    1182    2204    3183     934 
   Size:        248    1022     979    1251

  Fragments arranged by size: 

                1251    1022     979     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1342    1372    1374    1404    1342 
   Size:         30       2      30    3438

  Fragments arranged by size: 

                3438      30      30       2
 
HincII GTy'rAC

Cuts at:    539     539 
   Size:       3500
 
KasI G'GCGC_C

Cuts at:    930    1178     930 
   Size:        248    3252
 
KpnI G_GTAC'C

Cuts at:    520     520 
   Size:       3500
 
MluI A'CGCG_T

Cuts at:   2933    2933 
   Size:       3500
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   2171    2355    2171 
   Size:        184    3316
 
MscI TGG'CCA

Cuts at:    448    1261     448 
   Size:        813    2687
 
MslI CAynn'nnrTG

Cuts at:    130    1489    1616     130 
   Size:       1359     127    2014

  Fragments arranged by size: 

                2014    1359     127
 
MspA1I CmG'CkG

Cuts at:    130     218     611     925    1285    2298    2543     130 
   Size:         88     393     314     360    1013     245    1087

  Fragments arranged by size: 

                1087    1013     393     360     314     245      88
 
NaeI GCC'GGC

Cuts at:   1681    1681 
   Size:       3500
 
NarI GG'CG_CC

Cuts at:    931    1179     931 
   Size:        248    3252
 
NgoMIV G'CCGG_C

Cuts at:   1679    1679 
   Size:       3500
 
NspI r_CATG'y

Cuts at:    189     553    1053    1960     189 
   Size:        364     500     907    1729

  Fragments arranged by size: 

                1729     907     500     364
 
PasI CC'CwG_GG

Cuts at:    282     282 
   Size:       3500
 
PciI A'CATG_T

Cuts at:    185    1956     185 
   Size:       1771    1729
 
PflMI CCAn_nnn'nTGG

Cuts at:    155     155 
   Size:       3500
 
PfoI T'CCnGG_A

Cuts at:    404     404 
   Size:       3500
 
PmeI GTTT'AAAC

Cuts at:    499     499 
   Size:       3500
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2639    2671    2639 
   Size:         32    3468
 
PpuMI rG'GwC_Cy

Cuts at:    206     402     206 
   Size:        196    3304
 
PstI C_TGCA'G

Cuts at:    547    1232     547 
   Size:        685    2815
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       3500
 
PvuII CAG'CTG

Cuts at:    925    1285     925 
   Size:        360    3140
 
RsrII CG'GwC_CG

Cuts at:   1695    1695 
   Size:       3500
 
SacI G_AGCT'C

Cuts at:    514     514 
   Size:       3500
 
SalI G'TCGA_C

Cuts at:    537     537 
   Size:       3500
 
SapI GCTCTTCn'nnn_

Cuts at:   1523    1733    1523 
   Size:        210    3290
 
SbfI CC_TGCA'GG

Cuts at:    547     547 
   Size:       3500
 
ScaI AGT'ACT

Cuts at:   3017    3017 
   Size:       3500
 
SfcI C'TryA_G

Cuts at:     33     414     543    1228    2221    2412      33 
   Size:        381     129     685     993     191    1121

  Fragments arranged by size: 

                1121     993     685     381     191     129
 
SfoI GGC'GCC

Cuts at:    932    1180     932 
   Size:        248    3252
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3500
 
SmaI CCC'GGG

Cuts at:    400     522     400 
   Size:        122    3378
 
SmlI C'TyrA_G

Cuts at:    655    1570    2062    2324     655 
   Size:        915     492     262    1831

  Fragments arranged by size: 

                1831     915     492     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       3500
 
SphI G_CATG'C

Cuts at:    553    1053     553 
   Size:        500    3000
 
StuI AGG'CCT

Cuts at:    385     385 
   Size:       3500
 
StyI C'CwwG_G

Cuts at:    634     988     634 
   Size:        354    3146
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   1083    1216    1675    1884    1083 
   Size:        133     459     209    2699

  Fragments arranged by size: 

                2699     459     209     133
 
TatI w'GTAC_w

Cuts at:   3015    3015 
   Size:       3500
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    456     488    3017    3049     456 
   Size:         32    2529      32     907

  Fragments arranged by size: 

                2529     907      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   1297    1297 
   Size:       3500
 
XbaI T'CTAG_A

Cuts at:    531     531 
   Size:       3500
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:    995     995 
   Size:       3500
 
XmaI C'CCGG_G

Cuts at:    398     520     398 
   Size:        122    3378
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI   AflIII     AgeI     AleI    AlwNI
    ApaLI     ApoI     AseI    AsiSI     AvaI    BamHI     BanI    BanII
     BbeI     BcgI    BciVI     BclI    BglII     BlpI Bme1580I     BmrI
     BplI     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BseYI    BsiEI  BsiHKAI    BsmBI Bsp1286I    BspEI    BspHI
    BspMI    BsrBI    BsrDI    BsrFI    BssSI   BstAPI    BstBI    BstXI
    BstYI    BtgZI     BtsI     DraI     DrdI     EaeI     EagI     EarI
     EciI  EcoICRI  Eco57MI EcoO109I    EcoRI     FalI     FspI    HaeII
    Hin4I   HincII     KasI     KpnI     MluI     MmeI     MscI     MslI
   MspA1I     NaeI     NarI   NgoMIV     NspI     PasI     PciI    PflMI
     PfoI     PmeI     PpiI    PpuMI     PstI     PvuI    PvuII    RsrII
     SacI     SalI     SapI     SbfI     ScaI     SfcI     SfoI     SgfI
     SmaI     SmlI     SpeI     SphI     StuI     StyI    TaqII     TatI
     TstI  Tth111I     XbaI     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AfeI    AflII     AhdI     AlfI     AloI     ApaI
     AscI    AvrII     BaeI     BbsI    BbvCI    BfrBI     BglI    BmgBI
     BmtI   Bpu10I    BseRI     BsgI    BsiWI     BsmI    BsrGI   BssHII
   BstEII  BstZ17I   Bsu36I     BtgI     ClaI    CspCI   DraIII    EcoNI
    EcoRV     FseI    FspAI  HindIII     HpaI     MfeI     NcoI     NdeI
     NheI     NotI     NruI     NsiI     PacI     PmlI    PshAI     PsiI
   PspOMI    PspXI     PsrI    SacII    SanDI    SexAI     SfiI    SgrAI
    SnaBI     SrfI     SspI     SwaI     XhoI     XmnI     ZraI
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