Restriction map for pF1KB7083
##################################
# NO CUT ENZYMES (  165 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AflII       AflIII      AgeI        
AhdI        Alw44I      AlwNI       AocI        ApaI        
ApaLI       AscI        AseI        AsnI        Asp718I     
AspEI       AspHI       AspI        AvaI        AviII       
AvrII       BamHI       BbeI        BbrPI       BbsI        
BbvCI       BcgI        BciVI       BclI        BfaI        
BfrI        BglI        BglII       BlnI        BlpI        
Bpu1102I    BpuAI       BsaAI       BsaBI       BsaHI       
BsaI        BsaWI       BseAI       BseRI       BsgI        
BsiHKAI     BsiWI       BsmAI       BsmBI       BsmFI       
BsmI        BsoBI       BspDI       BspEI       BspLU11I    
BsrBI       BssHII      BssSI       Bst1107I    BstAPI      
BstBI       BstEII      BstPI       BstUI       BstZ17I     
Bsu36I      CelII       ClaI        CpoI        DraI        
DraII       DrdI        DsaI        Eam1105I    Eco81I      
EcoNI       EcoO109I    EcoO65I     EcoRV       EcoT22I     
FbaI        FseI        FspI        HgaI        Hin1I       
HindIII     HinfI       HpaI        KasI        KpnI        
KspI        MaeI        MaeII       MamI        MfeI        
MluI        MroI        MseI        MslI        MspA1I      
MunI        MvnI        NaeI        NarI        NcoI        
NdeI        NgoMIV      NheI        NotI        NruI        
NsiI        NspI        NspV        PacI        PaeR7I      
PflFI       PflMI       PinAI       PleI        PmaCI       
PmeI        PmlI        PpuMI       PshAI       PshBI       
Psp1406I    PstI        PvuI        PvuII       RsrII       
SacI        SacII       SalI        SapI        SbfI        
ScaI        SexAI       SfcI        SfiI        SfoI        
SfuI        SgrAI       SmaI        SmlI        SnaBI       
SpeI        SphI        Sse8387I    SspI        StuI        
SwaI        TfiI        Tru9I       Tth111I     Van91I      
XbaI        XcmI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   48 enzymes) #
###################################

AcsI          189  GAATTC               [GA]^AATT[CT]
Aor51HI       257  AGCGCT               AGC^GCT
ApoI          189  GAATTC               [GA]^AATT[CT]
Asp700I       189  GAATTCCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaII         144  GGTCC                G^G[AT]CC
BalI          320  TGGCCA               TGG^CCA
BanI          343  GGCACC               G^G[CT][GA]CC
BanII          39  GGGCTC               G[GA]GC[CT]^C
BcnI          310  CCGGG                CC^[GC]GG
BpmI          273  CTCCAG               CTGGAG(16/14)
BsiEI         290  CGGCCG               CG[GA][CT]^CG
Bsp1407I      125  TGTACA               T^GTACA
BspHI         185  TCATGA               T^CATGA
BspMI         141  GCAGGT               ACCTGC(4/8)
BsrDI         175  GCAATG               GCAATG(2/0)
BsrFI         157  ACCGGC               [GA]^CCGG[CT]
BsrGI         125  TGTACA               T^GTACA
BsrI          368  CCAGT                ACTGG(1/-1)
BstF5I        148  CATCC                GGATG(2/0)
BstXI         170  CCAAGGCAATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI         281  AGATCC               [GA]^GATC[CT]
Cfr10I        157  ACCGGC               [GA]^CCGG[CT]
Cfr13I        144  GGTCC                G^G[ACGT]CC
DraIII        328  CACGCCGTG            CAC[ACGT][ACGT][ACGT]^GTG
EagI          290  CGGCCG               C^GGCCG
EarI          165  CTCTTC               CTCTTC(1/4)
EclXI         290  CGGCCG               C^GGCCG
Eco47III      257  AGCGCT               AGC^GCT
Eco52I        290  CGGCCG               C^GGCCG
EcoRI         189  GAATTC               G^AATTC
FauI          236  CCCGC                CCCGC(4/6)
FokI          148  CATCC                GGATG(9/13)
HaeII         257  AGCGCT               [GA]GCGC^[CT]
HincII        199  GTCAAC               GT[CT]^[GA]AC
HindII        199  GTCAAC               GT[CT]^[GA]AC
Ksp632I       165  CTCTTC               CTCTTC(1/4)
MflI          281  AGATCC               [GA]^GATC[CT]
MluNI         320  TGGCCA               TGG^CCA
MscI          320  TGGCCA               TGG^CCA
NciI          310  CCGGG                CC^[GC]GG
NlaIII        186  CATG                 CATG^
RcaI          185  TCATGA               T^CATGA
Sau96I        144  GGTCC                G^G[ACGT]CC
SspBI         125  TGTACA               T^GTACA
Tsp509I       190  AATT                 ^AATT
TspRI         116  CAGTG                CA[GC]TG[ACGT][ACGT]^
XhoII         281  AGATCC               [GA]^GATC[CT]
XmnI          189  GAATTCCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   28 enzymes) #
###################################

AfaI          126  GTAC                 GT^AC
              363  GTAC                
AluI          109  AGCT                 AG^CT
              317  AGCT                
AlwI          282  GATCC                GGATC(4/5)
              182  GGATC               
BbvI           95  GCAGC                GCAGC(8/12)
              303  GCTGC               
BmyI           39  GGGCTC               G[AGT]GC[ACT]^C
              342  GGGCAC              
Bsp1286I       39  GGGCTC               G[AGT]GC[ACT]^C
              342  GGGCAC              
BstNI         240  CCTGG                CC^[AT]GG
              275  CCAGG               
DdeI           59  CTAAG                C^T[ACGT]AG
               65  CTCAG               
DpnII         183  GATC                 ^GATC
              282  GATC                
EaeI          290  CGGCCG               [CT]^GGCC[GA]
              320  TGGCCA              
EcoRII        240  CCTGG                ^CC[AT]GG
              275  CCAGG               
EcoT14I       170  CCAAGG               C^C[AT][AT]GG
              347  CCAAGG              
HapII         158  CCGG                 C^CGG
              310  CCGG                
HpaII         158  CCGG                 C^CGG
              310  CCGG                
MaeIII         55  GTGAC                ^GT[ACGT]AC
              355  GTCAC               
MboI          183  GATC                 ^GATC
              282  GATC                
MspI          158  CCGG                 C^CGG
              310  CCGG                
MvaI          240  CCTGG                CC^[AT]GG
              275  CCAGG               
NdeII         183  GATC                 ^GATC
              282  GATC                
NlaIV          40  GGCTCC               GG[ACGT]^[ACGT]CC
              343  GGCACC              
RsaI          126  GTAC                 GT^AC
              363  GTAC                
Sau3AI        183  GATC                 ^GATC
              282  GATC                
SfaNI         161  GCATC                GCATC(5/9)
              218  GCATC               
StyI          170  CCAAGG               C^C[AT][AT]GG
              347  CCAAGG              
TaqI          212  TCGA                 T^CGA
              262  TCGA                
TseI           95  GCAGC                G^C[AT]GC
              303  GCTGC               
Tsp45I         55  GTGAC                ^GT[GC]AC
              355  GTCAC               
TthHB8I       212  TCGA                 T^CGA
              262  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   17 enzymes) #
#########################################

AciI        BsaJI       BsiYI       BslI        BssKI       
Cac8I       CfoI        Fnu4HI      HaeIII      HhaI        
HinP1I      HphI        ItaI        MboII       MnlI        
MwoI        ScrFI       
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com