Restriction map for pF1KB7052
##################################
# NO CUT ENZYMES (  124 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AclI        
AfaI        AflII       AflIII      AgeI        AhdI        
Alw44I      Aor51HI     ApaLI       AscI        AseI        
AsnI        Asp718I     AspEI       AspI        AvrII       
BamHI       BbrPI       BbvCI       BcgI        BciVI       
BclI        BfrI        BglII       BlnI        BlpI        
Bpu1102I    BsaAI       BsaBI       BsiEI       BsiWI       
BsmBI       BsmI        Bsp1407I    BspHI       BspLU11I    
BsrDI       BsrGI       BssHII      BssSI       Bst1107I    
BstAPI      BstBI       BstEII      BstPI       BstUI       
BstXI       BstZ17I     CelII       CpoI        DraI        
DraIII      DrdI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      EcoO65I     EcoRI       EcoRV       
EcoT22I     FbaI        FseI        HincII      HindII      
HpaI        KpnI        KspI        MaeII       MamI        
MfeI        MluI        MseI        MunI        MvnI        
NaeI        NdeI        NgoMIV      NotI        NruI        
NsiI        NspV        PacI        PaeR7I      PflFI       
PinAI       PmaCI       PmeI        PmlI        PshAI       
PshBI       Psp1406I    PvuI        RcaI        RsaI        
RsrII       SacII       SalI        SapI        SbfI        
ScaI        SexAI       SfiI        SfuI        SgrAI       
SmaI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        SwaI        Tru9I       Tth111I     
XbaI        XhoI        XmaCI       XmaI        


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# ONE CUT ENZYMES (   30 enzymes) #
###################################

AccIII        113  TCCGGA               T^CCGGA
AcsI          310  GAATTT               [GA]^AATT[CT]
ApoI          310  GAATTT               [GA]^AATT[CT]
AvaI           10  CCCGAG               C^[CT]CG[GA]G
BalI         1464  TGGCCA               TGG^CCA
BbsI          532  GAAGAC               GAAGAC(2/6)
BpuAI         532  GAAGAC               GAAGAC(2/6)
BsaI         1533  GGTCTC               GGTCTC(1/5)
BsaWI         113  TCCGGA               [AT]^CCGG[AT]
BseAI         113  TCCGGA               T^CCGGA
BsmAI        1534  GTCTC                GTCTC(1/5)
BsoBI          10  CCCGAG               C^[CT]CG[GA]G
BspDI         928  ATCGAT               AT^CGAT
BspEI         113  TCCGGA               T^CCGGA
BsrBI        1020  CCGCTC               CCGCTC(-3/-3)
ClaI          928  ATCGAT               AT^CGAT
EarI          835  GAAGAG               CTCTTC(1/4)
HgaI          117  GACGC                GACGC(5/10)
Ksp632I       835  GAAGAG               CTCTTC(1/4)
MaeIII        101  GTGAC                ^GT[ACGT]AC
MluNI        1464  TGGCCA               TGG^CCA
MroI          113  TCCGGA               T^CCGGA
MscI         1464  TGGCCA               TGG^CCA
NheI          153  GCTAGC               G^CTAGC
PleI         1609  GAGTC                GAGTC(4/5)
SacI           55  GAGCTC               GAGCT^C
SmlI         1249  CTCAAG               C^T[CT][GA]AG
Tsp45I        101  GTGAC                ^GT[GC]AC
Tsp509I       311  AATT                 ^AATT
XcmI         1460  CCAATGGCCACCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   32 enzymes) #
###################################

AcyI          298  GGCGCC               G[GA]^CG[CT]C
             1382  GGCGCC              
AlwNI         481  CAGTATCTG            CAG[ACGT][ACGT][ACGT]^CTG
              773  CAGGAACTG           
Asp700I       180  GAAGCTTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              615  GAAAGCTTTC          
AspHI          55  GAGCTC               G[AT]GC[AT]^C
              361  GTGCTC              
AviII        1010  TGCGCA               TGC^GCA
             1370  TGCGCA              
BbeI          298  GGCGCC               GGCGC^C
             1382  GGCGCC              
BglI           83  GCCAGCTGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
              576  GCCACCTCGGC         
BsaHI         298  GGCGCC               G[GA]^CG[CT]C
             1382  GGCGCC              
BsiHKAI        55  GAGCTC               G[AT]GC[AT]^C
              361  GTGCTC              
BspMI        1265  ACCTGC               ACCTGC(4/8)
             1331  ACCTGC              
BsrFI         256  GCCGGT               [GA]^CCGG[CT]
             1280  ACCGGC              
BstYI         386  AGATCC               [GA]^GATC[CT]
             1169  AGATCC              
Cfr10I        256  GCCGGT               [GA]^CCGG[CT]
             1280  ACCGGC              
EaeI          236  CGGCCA               [CT]^GGCC[GA]
             1464  TGGCCA              
EcoNI         766  CCTCCCACAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
              960  CCTGCACCAGG         
FspI         1010  TGCGCA               TGC^GCA
             1370  TGCGCA              
Hin1I         298  GGCGCC               G[GA]^CG[CT]C
             1382  GGCGCC              
HindIII       181  AAGCTT               A^AGCTT
              617  AAGCTT              
KasI          298  GGCGCC               G^GCGCC
             1382  GGCGCC              
MflI          386  AGATCC               [GA]^GATC[CT]
             1169  AGATCC              
MslI           46  CACAGCAGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
               94  CACCTATGTG          
NarI          298  GGCGCC               GG^CGCC
             1382  GGCGCC              
NcoI         1065  CCATGG               C^CATGG
             1604  CCATGG              
PflMI         480  CCAGTATCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              856  CCAGCCTATGG         
PpuMI         795  GGGTCCC              [GA]G^G[AT]CC[CT]
             1096  AGGACCC             
PstI          421  CTGCAG               CTGCA^G
             1333  CTGCAG              
PvuII          85  CAGCTG               CAG^CTG
              356  CAGCTG              
SfoI          298  GGCGCC               GGC^GCC
             1382  GGCGCC              
StuI          491  AGGCCT               AGG^CCT
              984  AGGCCT              
Van91I        480  CCAGTATCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              856  CCAGCCTATGG         
XhoII         386  AGATCC               [GA]^GATC[CT]
             1169  AGATCC              
XmnI          180  GAAGCTTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              615  GAAAGCTTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   72 enzymes) #
#########################################

AciI        AluI        AlwI        AocI        ApaI        
AvaII       BanI        BanII       BbvI        BcnI        
BfaI        BmyI        BpmI        BsaJI       BseRI       
BsgI        BsiYI       BslI        BsmFI       Bsp1286I    
BsrI        BssKI       BstF5I      BstNI       Bsu36I      
Cac8I       CfoI        Cfr13I      DdeI        DpnII       
DraII       DsaI        Eco81I      EcoO109I    EcoRII      
EcoT14I     FauI        Fnu4HI      FokI        HaeII       
HaeIII      HapII       HhaI        HinP1I      HinfI       
HpaII       HphI        ItaI        MaeI        MboI        
MboII       MnlI        MspA1I      MspI        MvaI        
MwoI        NciI        NdeII       NlaIII      NlaIV       
NspI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        StyI        TaqI        TfiI        TseI        
TspRI       TthHB8I     
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