Restriction map for pF1KB7052
 (Linear) (Six-base) MAPSORT of: KIBB7052.seq  Check: 7576  from: 1  to: 1617



KIBB7052.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 10:58  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1274    1340    1617 
   Size:       1274      66     277

  Fragments arranged by size: 

                1274     277      66
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1186    1616    1617 
   Size:       1186     430       1

  Fragments arranged by size: 

                1186     430       1
 
AleI CACnn'nnGTG

Cuts at:      0      50      98    1617 
   Size:         50      48    1519

  Fragments arranged by size: 

                1519      50      48
 
AlwNI CAG_nnn'CTG

Cuts at:      0     486     778    1617 
   Size:        486     292     839

  Fragments arranged by size: 

                 839     486     292
 
ApaI G_GGCC'C

Cuts at:      0     887    1529    1550    1617 
   Size:        887     642      21      67

  Fragments arranged by size: 

                 887     642      67      21
 
ApoI r'AATT_y

Cuts at:      0     310    1617 
   Size:        310    1307
 
AvaI C'yCGr_G

Cuts at:      0      10    1617 
   Size:         10    1607
 
BanI G'GyrC_C

Cuts at:      0     298     549     666     978    1382    1575    1600    1617 
   Size:        298     251     117     312     404     193      25      17

  Fragments arranged by size: 

                 404     312     298     251     193     117      25      17
            
 
BanII G_rGCy'C

Cuts at:      0      10      59     477     887     913    1529    1550    1617 
   Size:         10      49     418     410      26     616      21      67

  Fragments arranged by size: 

                 616     418     410      67      49      26      21      10
            
 
BbeI G_GCGC'C

Cuts at:      0     302    1386    1617 
   Size:        302    1084     231

  Fragments arranged by size: 

                1084     302     231
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     539    1617 
   Size:        539    1078
 
BglI GCCn_nnn'nGGC

Cuts at:      0      89     582    1617 
   Size:         89     493    1035

  Fragments arranged by size: 

                1035     493      89
 
Bme1580I G_kGCm'C

Cuts at:      0     554     671     680     887    1045    1529    1550    1578 
   Size:        554     117       9     207     158     484      21      28

Cuts at:   1578    1617 
   Size:         39

  Fragments arranged by size: 

                 554     484     207     158     117      39      28      21
                   9
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     251    1532    1617 
   Size:        251    1281      85

  Fragments arranged by size: 

                1281     251      85
 
BmtI G_CTAG'C

Cuts at:      0     157    1617 
   Size:        157    1460
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     927    1617 
   Size:        927     690
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     349     403     508     536    1384    1617 
   Size:        349      54     105      28     848     233

  Fragments arranged by size: 

                 848     349     233     105      54      28
 
Bpu10I CC'TnA_GC

Cuts at:      0      61     952    1617 
   Size:         61     891     665

  Fragments arranged by size: 

                 891     665      61
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1234    1617 
   Size:       1234     383
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1539    1617 
   Size:       1539      78
 
BsaHI Gr'CG_yC

Cuts at:      0     299    1383    1617 
   Size:        299    1084     234

  Fragments arranged by size: 

                1084     299     234
 
BsaWI w'CCGG_w

Cuts at:      0     113    1617 
   Size:        113    1504
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     376     406     940     970    1617 
   Size:        376      30     534      30     647

  Fragments arranged by size: 

                 647     534     376      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     715     718     721     853     916    1579    1617 
   Size:        715       3       3     132      63     663      38

  Fragments arranged by size: 

                 715     663     132      63      38       3       3
 
BseYI C'CCAG_C

Cuts at:      0      87     445     855     906    1221    1617 
   Size:         87     358     410      51     315     396

  Fragments arranged by size: 

                 410     396     358     315      87      51
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     946    1219    1252    1617 
   Size:        946     273      33     365

  Fragments arranged by size: 

                 946     365     273      33
 
BsiHKAI G_wGCw'C

Cuts at:      0      59     365    1617 
   Size:         59     306    1252

  Fragments arranged by size: 

                1252     306      59
 
Bsp1286I G_dGCh'C

Cuts at:      0      10      59     365     477     554     671     680     887 
   Size:         10      49     306     112      77     117       9     207

Cuts at:    887     913    1045    1529    1550    1578    1617 
   Size:         26     132     484      21      28      39

  Fragments arranged by size: 

                 484     306     207     132     117     112      77      49
                  39      28      26      21      10       9
 
BspEI T'CCGG_A

Cuts at:      0     113    1617 
   Size:        113    1504
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1274    1340    1617 
   Size:       1274      66     277

  Fragments arranged by size: 

                1274     277      66
 
BsrBI CCG'CTC

Cuts at:      0    1022    1617 
   Size:       1022     595
 
BsrFI r'CCGG_y

Cuts at:      0     256    1280    1617 
   Size:        256    1024     337

  Fragments arranged by size: 

                1024     337     256
 
BstYI r'GATC_y

Cuts at:      0     386    1169    1617 
   Size:        386     783     448

  Fragments arranged by size: 

                 783     448     386
 
Bsu36I CC'TnA_GG

Cuts at:      0     662     791    1034    1617 
   Size:        662     129     243     583

  Fragments arranged by size: 

                 662     583     243     129
 
BtgI C'CryG_G

Cuts at:      0     506    1065    1604    1617 
   Size:        506     559     539      13

  Fragments arranged by size: 

                 559     539     506      13
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     384    1617 
   Size:        384    1233
 
BtsI GCAGTG_nn'

Cuts at:      0      57     435     738     747    1215    1617 
   Size:         57     378     303       9     468     402

  Fragments arranged by size: 

                 468     402     378     303      57       9
 
ClaI AT'CG_AT

Cuts at:      0     929    1617 
   Size:        929     688
 
EaeI y'GGCC_r

Cuts at:      0     236    1464    1617 
   Size:        236    1228     153

  Fragments arranged by size: 

                1228     236     153
 
EarI CTCTTCn'nnn_

Cuts at:      0     830    1617 
   Size:        830     787
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     218     557    1471    1617 
   Size:        218     339     914     146

  Fragments arranged by size: 

                 914     339     218     146
 
EcoICRI GAG'CTC

Cuts at:      0      57    1617 
   Size:         57    1560
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     349     403     508     536    1186    1384    1616    1617 
   Size:        349      54     105      28     650     198     232       1

  Fragments arranged by size: 

                 650     349     232     198     105      54      28       1
            
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     770     964    1617 
   Size:        770     194     653

  Fragments arranged by size: 

                 770     653     194
 
EcoO109I rG'GnC_Cy

Cuts at:      0     123     796     884    1097    1526    1546    1547    1617 
   Size:        123     673      88     213     429      20       1      70

  Fragments arranged by size: 

                 673     429     213     123      88      70      20       1
            
 
FspI TGC'GCA

Cuts at:      0    1012    1372    1617 
   Size:       1012     360     245

  Fragments arranged by size: 

                1012     360     245
 
FspAI rTGC'GCAy

Cuts at:      0    1372    1617 
   Size:       1372     245
 
HaeII r_GCGC'y

Cuts at:      0      84     302     347    1200    1341    1386    1617 
   Size:         84     218      45     853     141      45     231

  Fragments arranged by size: 

                 853     231     218     141      84      45      45
 
HindIII A'AGCT_T

Cuts at:      0     181     617    1617 
   Size:        181     436    1000

  Fragments arranged by size: 

                1000     436     181
 
KasI G'GCGC_C

Cuts at:      0     298    1382    1617 
   Size:        298    1084     235

  Fragments arranged by size: 

                1084     298     235
 
MscI TGG'CCA

Cuts at:      0    1466    1617 
   Size:       1466     151
 
MslI CAynn'nnrTG

Cuts at:      0      50      98    1617 
   Size:         50      48    1519

  Fragments arranged by size: 

                1519      50      48
 
MspA1I CmG'CkG

Cuts at:      0      87     358     891    1429    1444    1617 
   Size:         87     271     533     538      15     173

  Fragments arranged by size: 

                 538     533     271     173      87      15
 
NarI GG'CG_CC

Cuts at:      0     299    1383    1617 
   Size:        299    1084     234

  Fragments arranged by size: 

                1084     299     234
 
NcoI C'CATG_G

Cuts at:      0    1065    1604    1617 
   Size:       1065     539      13

  Fragments arranged by size: 

                1065     539      13
 
NheI G'CTAG_C

Cuts at:      0     153    1617 
   Size:        153    1464
 
NspI r_CATG'y

Cuts at:      0     354    1179    1263    1617 
   Size:        354     825      84     354

  Fragments arranged by size: 

                 825     354     354      84
 
PasI CC'CwG_GG

Cuts at:      0    1543    1617 
   Size:       1543      74
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     486     862    1617 
   Size:        486     376     755

  Fragments arranged by size: 

                 755     486     376
 
PfoI T'CCnGG_A

Cuts at:      0      38    1617 
   Size:         38    1579
 
PpuMI rG'GwC_Cy

Cuts at:      0     796    1097    1617 
   Size:        796     301     520

  Fragments arranged by size: 

                 796     520     301
 
PspOMI G'GGCC_C

Cuts at:      0     883    1525    1546    1617 
   Size:        883     642      21      71

  Fragments arranged by size: 

                 883     642      71      21
 
PstI C_TGCA'G

Cuts at:      0     425    1337    1617 
   Size:        425     912     280

  Fragments arranged by size: 

                 912     425     280
 
PvuII CAG'CTG

Cuts at:      0      87     358    1617 
   Size:         87     271    1259

  Fragments arranged by size: 

                1259     271      87
 
SacI G_AGCT'C

Cuts at:      0      59    1617 
   Size:         59    1558
 
SanDI GG'GwC_CC

Cuts at:      0     796    1617 
   Size:        796     821
 
SfcI C'TryA_G

Cuts at:      0     162     421     759    1333    1617 
   Size:        162     259     338     574     284

  Fragments arranged by size: 

                 574     338     284     259     162
 
SfoI GGC'GCC

Cuts at:      0     300    1384    1617 
   Size:        300    1084     233

  Fragments arranged by size: 

                1084     300     233
 
SmlI C'TyrA_G

Cuts at:      0    1249    1617 
   Size:       1249     368
 
StuI AGG'CCT

Cuts at:      0     493     986    1617 
   Size:        493     493     631

  Fragments arranged by size: 

                 631     493     493
 
StyI C'CwwG_G

Cuts at:      0     632    1065    1319    1604    1617 
   Size:        632     433     254     285      13

  Fragments arranged by size: 

                 632     433     285     254      13
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     154    1593    1617 
   Size:        154    1439      24

  Fragments arranged by size: 

                1439     154      24
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     376     408    1617 
   Size:        376      32    1209

  Fragments arranged by size: 

                1209     376      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1467    1617 
   Size:       1467     150
 
XmnI GAAnn'nnTTC

Cuts at:      0     184     619    1617 
   Size:        184     435     998

  Fragments arranged by size: 

                 998     435     184
 

 Enzymes that do cut:

     AarI     AcuI     AleI    AlwNI     ApaI     ApoI     AvaI     BanI
    BanII     BbeI     BbsI     BglI Bme1580I     BmrI     BmtI     BplI
     BpmI   Bpu10I    BpuEI     BsaI    BsaHI    BsaWI    BsaXI    BseRI
    BseYI     BsgI  BsiHKAI Bsp1286I    BspEI    BspMI    BsrBI    BsrFI
    BstYI   Bsu36I     BtgI    BtgZI     BtsI     ClaI     EaeI     EarI
     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I     FspI    FspAI    HaeII
  HindIII     KasI     MscI     MslI   MspA1I     NarI     NcoI     NheI
     NspI     PasI    PflMI     PfoI    PpuMI   PspOMI     PstI    PvuII
     SacI    SanDI     SfcI     SfoI     SmlI     StuI     StyI    TaqII
     TstI     XcmI     XmnI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AlfI     AloI    ApaLI     AscI     AseI    AsiSI    AvrII
     BaeI    BamHI    BbvCI     BcgI    BciVI     BclI    BfrBI    BglII
     BlpI    BmgBI    BsaAI    BsaBI    BsiEI    BsiWI     BsmI    BsmBI
    BspHI    BsrDI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII
    BstXI  BstZ17I    CspCI     DraI   DraIII     DrdI     EagI    EcoRI
    EcoRV     FalI     FseI    Hin4I   HincII     HpaI     KpnI     MfeI
     MluI     MmeI     NaeI     NdeI   NgoMIV     NotI     NruI     NsiI
     PacI     PciI     PmeI     PmlI     PpiI    PshAI     PsiI    PspXI
     PsrI     PvuI    RsrII    SacII     SalI     SapI     SbfI     ScaI
    SexAI     SfiI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI
     SrfI     SspI     SwaI     TatI  Tth111I     XbaI     XhoI     XmaI
     ZraI
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