Restriction map for pF1KB7051
##################################
# NO CUT ENZYMES (  101 enzymes) #
##################################

AatII       Acc65I      AclI        AflII       AgeI        
AhdI        Aor51HI     ApaI        AscI        AseI        
AsnI        Asp718I     AspEI       AspI        AviII       
AvrII       BamHI       BanII       BbsI        BcgI        
BciVI       BclI        BfrI        BglII       BlnI        
BpuAI       BsaBI       BsiEI       BsiWI       BsmBI       
BsrFI       BssHII      BssSI       BstAPI      BstBI       
BstEII      BstPI       Cfr10I      CpoI        DraI        
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
EcoO65I     EcoRI       EcoRV       EcoT22I     FbaI        
FseI        FspI        HincII      HindII      HpaI        
KpnI        KspI        MamI        MfeI        MluI        
MunI        NaeI        NdeI        NgoMIV      NheI        
NotI        NruI        NsiI        NspV        PacI        
PaeR7I      PflFI       PflMI       PinAI       PmeI        
PshAI       PshBI       Psp1406I    PvuI        RsrII       
SacI        SacII       SalI        SapI        SbfI        
SexAI       SfiI        SfuI        SgrAI       SmlI        
SnaBI       SpeI        SphI        Sse8387I    SspI        
SwaI        Tth111I     Van91I      XbaI        XcmI        
XhoI        


###################################
# ONE CUT ENZYMES (   65 enzymes) #
###################################

AccI           97  GTATAC               GT^[AC][GT]AC
AccII         868  CGCG                 CG^CG
AccIII        794  TCCGGA               T^CCGGA
AcsI         1111  AAATTT               [GA]^AATT[CT]
AcyI          255  GGCGCC               G[GA]^CG[CT]C
Alw44I       1092  GTGCAC               G^TGCAC
AocI          514  CCTGAGG              CC^T[ACGT]AGG
ApaLI        1092  GTGCAC               G^TGCAC
ApoI         1111  AAATTT               [GA]^AATT[CT]
Asp700I      1161  GAAGCCTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI        1092  GTGCAC               G[AT]GC[AT]^C
AvaII         510  GGTCC                G^G[AT]CC
BalI            8  TGGCCA               TGG^CCA
BanI          255  GGCGCC               G^G[CT][GA]CC
BbeI          255  GGCGCC               GGCGC^C
BbrPI        1222  CACGTG               CAC^GTG
BbvCI         259  CCTCAGC              CCTCAGC(-5/-2)
BglI         1204  GCCAACCAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI           46  GCTCAGC              GC^T[ACGT]AGC
BmyI         1092  GTGCAC               G[AGT]GC[ACT]^C
Bpu1102I       46  GCTCAGC              GC^T[ACGT]AGC
BsaHI         255  GGCGCC               G[GA]^CG[CT]C
BsaI          655  GAGACC               GGTCTC(1/5)
BsaWI         794  TCCGGA               [AT]^CCGG[AT]
BseAI         794  TCCGGA               T^CCGGA
BsiHKAI      1092  GTGCAC               G[AT]GC[AT]^C
BsmI          401  GCATTC               GAATGC(1/-1)
Bsp1286I     1092  GTGCAC               G[AGT]GC[ACT]^C
BspDI         985  ATCGAT               AT^CGAT
BspEI         794  TCCGGA               T^CCGGA
BspHI         728  TCATGA               T^CATGA
BspLU11I     1142  ACATGT               A^CATGT
BspMI         507  GCAGGT               ACCTGC(4/8)
BsrBI         316  CCGCTC               CCGCTC(-3/-3)
Bst1107I       97  GTATAC               GTA^TAC
BstUI         868  CGCG                 CG^CG
BstZ17I        97  GTATAC               GTA^TAC
Bsu36I        514  CCTGAGG              CC^T[ACGT]AGG
CelII          46  GCTCAGC              GC^T[ACGT]AGC
ClaI          985  ATCGAT               AT^CGAT
DraII         509  AGGTCCC              [GA]G^G[ACGT]CC[CT]
DraIII       1492  CACCCAGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI          597  GACCTCGCTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I        514  CCTGAGG              CC^T[ACGT]AGG
EcoNI        1044  CCTGGAGCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I      509  AGGTCCC              [GA]G^G[ACGT]CC[CT]
Hin1I         255  GGCGCC               G[GA]^CG[CT]C
HindIII      1191  AAGCTT               A^AGCTT
KasI          255  GGCGCC               G^GCGCC
MluNI           8  TGGCCA               TGG^CCA
MroI          794  TCCGGA               T^CCGGA
MscI            8  TGGCCA               TGG^CCA
MvnI          868  CGCG                 CG^CG
NarI          255  GGCGCC               GG^CGCC
PmaCI        1222  CACGTG               CAC^GTG
PmlI         1222  CACGTG               CAC^GTG
PpuMI         509  AGGTCCC              [GA]G^G[AT]CC[CT]
RcaI          728  TCATGA               T^CATGA
ScaI          755  AGTACT               AGT^ACT
SfoI          255  GGCGCC               GGC^GCC
SmaI          646  CCCGGG               CCC^GGG
StuI          518  AGGCCT               AGG^CCT
XmaCI         646  CCCGGG               C^CCGGG
XmaI          646  CCCGGG               C^CCGGG
XmnI         1161  GAAGCCTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   28 enzymes) #
###################################

AflIII       1142  ACATGT               A^C[GA][CT]GT
             1223  ACGTGT              
AlwNI         449  CAGCACCTG            CAG[ACGT][ACGT][ACGT]^CTG
             1270  CAGACTCTG           
BsaAI         806  TACGTG               [CT]AC^GT[GA]
             1222  CACGTG              
BseRI         641  CTCCTC               GAGGAG(10/8)
              652  GAGGAG              
Bsp1407I      239  TGTACA               T^GTACA
             1145  TGTACA              
BsrDI        1026  CATTGC               GCAATG(2/0)
              890  GCAATG              
BsrGI         239  TGTACA               T^GTACA
             1145  TGTACA              
BstF5I        938  GGATG                GGATG(2/0)
             1419  GGATG               
BstXI         768  CCACCTGTGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1448  CCATTCTCCTGG        
BstYI         471  AGATCC               [GA]^GATC[CT]
              821  AGATCC              
CfoI          256  GCGC                 GCG^C
              783  GCGC                
EaeI            8  TGGCCA               [CT]^GGCC[GA]
              478  CGGCCA              
EarI          732  GAAGAG               CTCTTC(1/4)
             1074  GAAGAG              
FauI          315  CCCGC                CCCGC(4/6)
             1217  CCCGC               
FokI          938  GGATG                GGATG(9/13)
             1419  GGATG               
HaeII         255  GGCGCC               [GA]GCGC^[CT]
              782  GGCGCT              
HhaI          256  GCGC                 GCG^C
              783  GCGC                
HinP1I        256  GCGC                 G^CGC
              783  GCGC                
Ksp632I       732  GAAGAG               CTCTTC(1/4)
             1074  GAAGAG              
MflI          471  AGATCC               [GA]^GATC[CT]
              821  AGATCC              
MspA1I        535  CAGCTG               C[AC]G^C[GT]G
              896  CAGCTG              
NspI         1142  ACATGT               [GA]CATG^[CT]
             1316  ACATGC              
PstI          592  CTGCAG               CTGCA^G
              958  CTGCAG              
PvuII         535  CAGCTG               CAG^CTG
              896  CAGCTG              
SspBI         239  TGTACA               T^GTACA
             1145  TGTACA              
TfiI          561  GAATC                G^A[AT]TC
              973  GATTC               
Tsp509I       139  AATT                 ^AATT
             1112  AATT                
XhoII         471  AGATCC               [GA]^GATC[CT]
              821  AGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   64 enzymes) #
#########################################

AciI        AfaI        AluI        AlwI        AvaI        
BbvI        BcnI        BfaI        BpmI        BsaJI       
BsgI        BsiYI       BslI        BsmAI       BsmFI       
BsoBI       BsrI        BssKI       BstNI       Cac8I       
Cfr13I      DdeI        DpnII       DsaI        EcoRII      
EcoT14I     Fnu4HI      HaeIII      HapII       HgaI        
HinfI       HpaII       HphI        ItaI        MaeI        
MaeII       MaeIII      MboI        MboII       MnlI        
MseI        MslI        MspI        MvaI        MwoI        
NciI        NcoI        NdeII       NlaIII      NlaIV       
PleI        RsaI        Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        StyI        TaqI        Tru9I       
TseI        Tsp45I      TspRI       TthHB8I     
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