Restriction map for pF1KB7051
 (Linear) (Six-base) MAPSORT of: KIBB7051.seq  Check: 6027  from: 1  to: 1542



KIBB7051.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 10:58  ..

AccI GT'mk_AC

Cuts at:      0      98    1542 
   Size:         98    1444
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     201     431     950    1542 
   Size:        201     230     519     592

  Fragments arranged by size: 

                 592     519     230     201
 
AflIII A'CryG_T

Cuts at:      0    1142    1223    1542 
   Size:       1142      81     319

  Fragments arranged by size: 

                1142     319      81
 
AleI CACnn'nnGTG

Cuts at:      0     773    1258    1542 
   Size:        773     485     284

  Fragments arranged by size: 

                 773     485     284
 
AlwNI CAG_nnn'CTG

Cuts at:      0     454    1275    1542 
   Size:        454     821     267

  Fragments arranged by size: 

                 821     454     267
 
ApaLI G'TGCA_C

Cuts at:      0    1092    1542 
   Size:       1092     450
 
ApoI r'AATT_y

Cuts at:      0    1111    1542 
   Size:       1111     431
 
AvaI C'yCGr_G

Cuts at:      0     463     646     904    1542 
   Size:        463     183     258     638

  Fragments arranged by size: 

                 638     463     258     183
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     818     851    1542 
   Size:        818      33     691

  Fragments arranged by size: 

                 818     691      33
 
BanI G'GyrC_C

Cuts at:      0     255    1542 
   Size:        255    1287
 
BbeI G_GCGC'C

Cuts at:      0     259    1542 
   Size:        259    1283
 
BbvCI CC'TCA_GC

Cuts at:      0     260    1542 
   Size:        260    1282
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1210    1542 
   Size:       1210     332
 
BlpI GC'TnA_GC

Cuts at:      0      47    1542 
   Size:         47    1495
 
Bme1580I G_kGCm'C

Cuts at:      0    1096    1542 
   Size:       1096     446
 
BmgBI CAC'GTC

Cuts at:      0     714    1542 
   Size:        714     828
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     254    1489    1542 
   Size:        254    1235      53

  Fragments arranged by size: 

                1235     254      53
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     474     593     802    1066    1152    1542 
   Size:        474     119     209     264      86     390

  Fragments arranged by size: 

                 474     390     264     209     119      86
 
Bpu10I CC'TnA_GC

Cuts at:      0     260    1542 
   Size:        260    1282
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     649    1542 
   Size:        649     893
 
BsaAI yAC'GTr

Cuts at:      0     808    1224    1542 
   Size:        808     416     318

  Fragments arranged by size: 

                 808     416     318
 
BsaHI Gr'CG_yC

Cuts at:      0     256    1542 
   Size:        256    1286
 
BsaWI w'CCGG_w

Cuts at:      0     794    1542 
   Size:        794     748
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     632     667    1542 
   Size:        632      35     875

  Fragments arranged by size: 

                 875     632      35
 
BseYI C'CCAG_C

Cuts at:      0     533    1542 
   Size:        533    1009
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      60     289    1194    1269    1542 
   Size:         60     229     905      75     273

  Fragments arranged by size: 

                 905     273     229      75      60
 
BsiHKAI G_wGCw'C

Cuts at:      0    1096    1542 
   Size:       1096     446
 
BsmI GAATG_Cn'

Cuts at:      0     401    1542 
   Size:        401    1141
 
Bsp1286I G_dGCh'C

Cuts at:      0    1096    1542 
   Size:       1096     446
 
BspEI T'CCGG_A

Cuts at:      0     794    1542 
   Size:        794     748
 
BspHI T'CATG_A

Cuts at:      0     728    1542 
   Size:        728     814
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     498    1542 
   Size:        498    1044
 
BsrBI CCG'CTC

Cuts at:      0     318    1542 
   Size:        318    1224
 
BsrDI GCAATG_nn'

Cuts at:      0     897    1025    1542 
   Size:        897     128     517

  Fragments arranged by size: 

                 897     517     128
 
BsrGI T'GTAC_A

Cuts at:      0     239    1145    1542 
   Size:        239     906     397

  Fragments arranged by size: 

                 906     397     239
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     775    1455    1542 
   Size:        775     680      87

  Fragments arranged by size: 

                 775     680      87
 
BstYI r'GATC_y

Cuts at:      0     471     821    1542 
   Size:        471     350     721

  Fragments arranged by size: 

                 721     471     350
 
BstZ17I GTA'TAC

Cuts at:      0      99    1542 
   Size:         99    1443
 
Bsu36I CC'TnA_GG

Cuts at:      0     515    1542 
   Size:        515    1027
 
BtgI C'CryG_G

Cuts at:      0      15     475     917    1256    1542 
   Size:         15     460     442     339     286

  Fragments arranged by size: 

                 460     442     339     286      15
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     107    1542 
   Size:        107    1435
 
BtsI GCAGTG_nn'

Cuts at:      0    1467    1542 
   Size:       1467      75
 
ClaI AT'CG_AT

Cuts at:      0     986    1542 
   Size:        986     556
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     423     458    1542 
   Size:        423      35    1084

  Fragments arranged by size: 

                1084     423      35
 
DraIII CAC_nnn'GTG

Cuts at:      0    1497    1542 
   Size:       1497      45
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     603    1542 
   Size:        603     939
 
EaeI y'GGCC_r

Cuts at:      0       8     478    1542 
   Size:          8     470    1064

  Fragments arranged by size: 

                1064     470       8
 
EarI CTCTTCn'nnn_

Cuts at:      0     727    1069    1542 
   Size:        727     342     473

  Fragments arranged by size: 

                 727     473     342
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    1095    1542 
   Size:       1095     447
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     201     431     474     593     802     950    1066    1152 
   Size:        201     230      43     119     209     148     116      86

Cuts at:   1152    1542 
   Size:        390

  Fragments arranged by size: 

                 390     230     209     201     148     119     116      86
                  43
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1048    1542 
   Size:       1048     494
 
EcoO109I rG'GnC_Cy

Cuts at:      0     510    1542 
   Size:        510    1032
 
HaeII r_GCGC'y

Cuts at:      0     259     786    1542 
   Size:        259     527     756

  Fragments arranged by size: 

                 756     527     259
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     243     275     399     431     531     563    1436    1468 
   Size:        243      32     124      32     100      32     873      32

Cuts at:   1468    1542 
   Size:         74

  Fragments arranged by size: 

                 873     243     124     100      74      32      32      32
                  32
 
HindIII A'AGCT_T

Cuts at:      0    1191    1542 
   Size:       1191     351
 
KasI G'GCGC_C

Cuts at:      0     255    1542 
   Size:        255    1287
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     983    1542 
   Size:        983     559
 
MscI TGG'CCA

Cuts at:      0      10    1542 
   Size:         10    1532
 
MslI CAynn'nnrTG

Cuts at:      0     773     922    1258    1321    1542 
   Size:        773     149     336      63     221

  Fragments arranged by size: 

                 773     336     221     149      63
 
MspA1I CmG'CkG

Cuts at:      0     537     898    1542 
   Size:        537     361     644

  Fragments arranged by size: 

                 644     537     361
 
NarI GG'CG_CC

Cuts at:      0     256    1542 
   Size:        256    1286
 
NcoI C'CATG_G

Cuts at:      0      15     917    1256    1542 
   Size:         15     902     339     286

  Fragments arranged by size: 

                 902     339     286      15
 
NspI r_CATG'y

Cuts at:      0    1146    1320    1542 
   Size:       1146     174     222

  Fragments arranged by size: 

                1146     222     174
 
PciI A'CATG_T

Cuts at:      0    1142    1542 
   Size:       1142     400
 
PfoI T'CCnGG_A

Cuts at:      0     490    1542 
   Size:        490    1052
 
PmlI CAC'GTG

Cuts at:      0    1224    1542 
   Size:       1224     318
 
PpuMI rG'GwC_Cy

Cuts at:      0     510    1542 
   Size:        510    1032
 
PstI C_TGCA'G

Cuts at:      0     596     962    1542 
   Size:        596     366     580

  Fragments arranged by size: 

                 596     580     366
 
PvuII CAG'CTG

Cuts at:      0     537     898    1542 
   Size:        537     361     644

  Fragments arranged by size: 

                 644     537     361
 
ScaI AGT'ACT

Cuts at:      0     757    1542 
   Size:        757     785
 
SfcI C'TryA_G

Cuts at:      0     592     885     958    1542 
   Size:        592     293      73     584

  Fragments arranged by size: 

                 592     584     293      73
 
SfoI GGC'GCC

Cuts at:      0     257    1542 
   Size:        257    1285
 
SmaI CCC'GGG

Cuts at:      0     648    1542 
   Size:        648     894
 
StuI AGG'CCT

Cuts at:      0     520    1542 
   Size:        520    1022
 
StyI C'CwwG_G

Cuts at:      0      15     917    1256    1542 
   Size:         15     902     339     286

  Fragments arranged by size: 

                 902     339     286      15
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1508    1542 
   Size:       1508      34
 
TatI w'GTAC_w

Cuts at:      0     239     755    1145    1542 
   Size:        239     516     390     397

  Fragments arranged by size: 

                 516     397     390     239
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     615     647     930     962    1542 
   Size:        615      32     283      32     580

  Fragments arranged by size: 

                 615     580     283      32      32
 
XmaI C'CCGG_G

Cuts at:      0     646    1542 
   Size:        646     896
 
XmnI GAAnn'nnTTC

Cuts at:      0    1165    1542 
   Size:       1165     377
 

 Enzymes that do cut:

     AccI     AcuI   AflIII     AleI    AlwNI    ApaLI     ApoI     AvaI
     BaeI     BanI     BbeI    BbvCI     BglI     BlpI Bme1580I    BmgBI
     BmrI     BpmI   Bpu10I     BsaI    BsaAI    BsaHI    BsaWI    BseRI
    BseYI     BsgI  BsiHKAI     BsmI Bsp1286I    BspEI    BspHI    BspMI
    BsrBI    BsrDI    BsrGI    BstXI    BstYI  BstZ17I   Bsu36I     BtgI
    BtgZI     BtsI     ClaI    CspCI   DraIII     DrdI     EaeI     EarI
     EciI  Eco57MI    EcoNI EcoO109I    HaeII    Hin4I  HindIII     KasI
     MmeI     MscI     MslI   MspA1I     NarI     NcoI     NspI     PciI
     PfoI     PmlI    PpuMI     PstI    PvuII     ScaI     SfcI     SfoI
     SmaI     StuI     StyI    TaqII     TatI     TstI     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII     AgeI     AhdI
     AlfI     AloI     ApaI     AscI     AseI    AsiSI    AvrII    BamHI
    BanII     BbsI     BcgI    BciVI     BclI    BfrBI    BglII     BmtI
     BplI    BpuEI    BsaBI    BsaXI    BsiEI    BsiWI    BsmBI    BsrFI
   BssHII    BssSI   BstAPI    BstBI   BstEII     DraI     EagI  EcoICRI
    EcoRI    EcoRV     FalI     FseI     FspI    FspAI   HincII     HpaI
     KpnI     MfeI     MluI     NaeI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     PacI     PasI    PflMI     PmeI     PpiI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PvuI    RsrII     SacI    SacII
     SalI    SanDI     SapI     SbfI    SexAI     SfiI     SgfI    SgrAI
     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     SwaI  Tth111I
     XbaI     XcmI     XhoI     ZraI
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