Restriction map for pF1KB7045
##################################
# NO CUT ENZYMES (  129 enzymes) #
##################################

AatII       AccII       AccIII      AclI        AcsI        
AflII       AflIII      AhdI        AlwNI       AocI        
Aor51HI     ApoI        AscI        AseI        AsnI        
AspEI       AspI        AvaI        AvrII       BamHI       
BbeI        BbrPI       BbsI        BbvCI       BcgI        
BciVI       BfrI        BglII       BlnI        BpuAI       
BsaAI       BsaBI       BseAI       BsiWI       BsmI        
BsoBI       Bsp1407I    BspDI       BspEI       BspHI       
BspLU11I    BsrDI       BsrGI       BssHII      Bst1107I    
BstAPI      BstBI       BstUI       BstZ17I     Bsu36I      
ClaI        CpoI        DraI        DraIII      DrdI        
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
Eco81I      EcoRI       EcoRV       EcoT22I     FseI        
HincII      HindII      HindIII     HpaI        KasI        
KspI        MamI        MfeI        MluI        MroI        
MseI        MunI        MvnI        NaeI        NarI        
NcoI        NdeI        NgoMIV      NheI        NotI        
NruI        NsiI        NspV        PacI        PaeR7I      
PflFI       PflMI       PleI        PmaCI       PmeI        
PmlI        PshBI       Psp1406I    PstI        PvuI        
RcaI        RsrII       SacI        SacII       SalI        
SapI        SbfI        ScaI        SexAI       SfiI        
SfoI        SfuI        SmaI        SmlI        SnaBI       
SpeI        Sse8387I    SspBI       SwaI        TaqI        
Tru9I       Tsp509I     Tth111I     TthHB8I     Van91I      
XbaI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   44 enzymes) #
###################################

Acc65I       1351  GGTACC               G^GTACC
AccI           19  GTAGAC               GT^[AC][GT]AC
AcyI          996  GACGCC               G[GA]^CG[CT]C
AgeI         1177  ACCGGT               A^CCGGT
ApaI          822  GGGCCC               GGGCC^C
Asp700I      1185  GAAGCCCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I      1351  GGTACC               G^GTACC
AviII        1127  TGCGCA               TGC^GCA
BalI          431  TGGCCA               TGG^CCA
BanII         822  GGGCCC               G[GA]GC[CT]^C
BclI          176  TGATCA               T^GATCA
BfaI          254  CTAG                 C^TAG
BglI          945  GCCACTGCGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI          893  GCTCAGC              GC^T[ACGT]AGC
BpmI          194  CTGGAG               CTGGAG(16/14)
Bpu1102I      893  GCTCAGC              GC^T[ACGT]AGC
BsaHI         996  GACGCC               G[GA]^CG[CT]C
BsiEI        1041  CGACCG               CG[GA][CT]^CG
BsmBI         243  CGTCTC               CGTCTC(1/5)
BspMI         941  ACCTGC               ACCTGC(4/8)
BssSI        1006  CACGAG               CACGAG(-5/-1)
BstXI         422  CCAACCCACTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII         893  GCTCAGC              GC^T[ACGT]AGC
DsaI          641  CCACGG               C^C[GA][CT]GG
EcoNI         540  CCTCTACCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I        11  CCAAGG               C^C[AT][AT]GG
FbaI          176  TGATCA               T^GATCA
FspI         1127  TGCGCA               TGC^GCA
HaeII        1241  AGCGCC               [GA]GCGC^[CT]
Hin1I         996  GACGCC               G[GA]^CG[CT]C
KpnI         1351  GGTACC               GGTAC^C
MaeI          254  CTAG                 C^TAG
MaeII         100  ACGT                 A^CGT
MluNI         431  TGGCCA               TGG^CCA
MscI          431  TGGCCA               TGG^CCA
NspI         1124  GCATGC               [GA]CATG^[CT]
PinAI        1177  ACCGGT               A^CCGGT
PshAI         646  GACCCAGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SfcI         1316  CTACAG               C^T[GA][CT]AG
SphI         1124  GCATGC               GCATG^C
SspI          340  AATATT               AAT^ATT
StuI          964  AGGCCT               AGG^CCT
StyI           11  CCAAGG               C^C[AT][AT]GG
XmnI         1185  GAAGCCCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   15 enzymes) #
###################################

BsaWI         841  ACCGGA               [AT]^CCGG[AT]
             1177  ACCGGT              
BsmFI         870  GGGAC                GGGAC(10/14)
              462  GTCCC               
BstEII        105  GGTGACC              G^GT[ACGT]ACC
              837  GGTGACC             
BstPI         105  GGTGACC              G^GT[ACGT]ACC
              837  GGTGACC             
CfoI         1128  GCGC                 GCG^C
             1242  GCGC                
EcoO65I       105  GGTGACC              G^GT[ACGT]ACC
              837  GGTGACC             
FauI         1055  CCCGC                CCCGC(4/6)
              768  GCGGG               
HgaI          996  GACGC                GACGC(5/10)
              404  GCGTC               
HhaI         1128  GCGC                 GCG^C
             1242  GCGC                
HinP1I       1128  GCGC                 G^CGC
             1242  GCGC                
HinfI         683  GATTC                G^A[ACGT]TC
             1085  GAATC               
SfaNI         356  GCATC                GCATC(5/9)
             1297  GCATC               
SgrAI         998  CGCCGGTG             C[GA]^CCGG[CT]G
             1176  CACCGGTG            
TfiI          683  GATTC                G^A[AT]TC
             1085  GAATC               
XcmI          119  CCAATGCCGAACTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
              434  CCACAGGACCCCTGG     


#########################################
# 3 OR MORE CUT ENZYMES (   70 enzymes) #
#########################################

AciI        AfaI        AluI        Alw44I      AlwI        
ApaLI       AspHI       AvaII       BanI        BbvI        
BcnI        BmyI        BsaI        BsaJI       BseRI       
BsgI        BsiHKAI     BsiYI       BslI        BsmAI       
Bsp1286I    BsrBI       BsrFI       BsrI        BssKI       
BstF5I      BstNI       BstYI       Cac8I       Cfr10I      
Cfr13I      DdeI        DpnII       DraII       EaeI        
EarI        EcoO109I    EcoRII      Fnu4HI      FokI        
HaeIII      HapII       HpaII       HphI        ItaI        
Ksp632I     MaeIII      MboI        MboII       MflI        
MnlI        MslI        MspA1I      MspI        MvaI        
MwoI        NciI        NdeII       NlaIII      NlaIV       
PpuMI       PvuII       RsaI        Sau3AI      Sau96I      
ScrFI       TseI        Tsp45I      TspRI       XhoII       
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