Restriction map for pF1KB7045
 (Linear) (Six-base) MAPSORT of: KIBB7045.seq  Check: 1812  from: 1  to: 1428



KIBB7045.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 10:58  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     950    1428 
   Size:        950     478
 
AccI GT'mk_AC

Cuts at:      0      20    1428 
   Size:         20    1408
 
Acc65I G'GTAC_C

Cuts at:      0    1351    1428 
   Size:       1351      77
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1119    1428 
   Size:       1119     309
 
AgeI A'CCGG_T

Cuts at:      0    1177    1428 
   Size:       1177     251
 
AleI CACnn'nnGTG

Cuts at:      0     103    1178    1428 
   Size:        103    1075     250

  Fragments arranged by size: 

                1075     250     103
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1053    1428 
   Size:       1053     375
 
ApaI G_GGCC'C

Cuts at:      0     826    1428 
   Size:        826     602
 
ApaLI G'TGCA_C

Cuts at:      0      52     453    1003    1428 
   Size:         52     401     550     425

  Fragments arranged by size: 

                 550     425     401      52
 
BanI G'GyrC_C

Cuts at:      0     310     771    1302    1351    1428 
   Size:        310     461     531      49      77

  Fragments arranged by size: 

                 531     461     310      77      49
 
BanII G_rGCy'C

Cuts at:      0     826    1428 
   Size:        826     602
 
BclI T'GATC_A

Cuts at:      0     176    1428 
   Size:        176    1252
 
BglI GCCn_nnn'nGGC

Cuts at:      0     951    1428 
   Size:        951     477
 
BlpI GC'TnA_GC

Cuts at:      0     894    1428 
   Size:        894     534
 
Bme1580I G_kGCm'C

Cuts at:      0      56     457     776     826    1007    1027    1290    1428 
   Size:         56     401     319      50     181      20     263     138

  Fragments arranged by size: 

                 401     319     263     181     138      56      50      20
            
 
BmgBI CAC'GTC

Cuts at:      0     101    1428 
   Size:        101    1327
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      25     139     821    1103    1428 
   Size:         25     114     682     282     325

  Fragments arranged by size: 

                 682     325     282     114      25
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1411    1428 
   Size:       1411      17
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     215    1428 
   Size:        215    1213
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     530     658     701    1428 
   Size:        530     128      43     727

  Fragments arranged by size: 

                 727     530     128      43
 
BsaHI Gr'CG_yC

Cuts at:      0     997    1428 
   Size:        997     431
 
BsaWI w'CCGG_w

Cuts at:      0     841    1177    1428 
   Size:        841     336     251

  Fragments arranged by size: 

                 841     336     251
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     500     503    1398    1428 
   Size:        500       3     895      30

  Fragments arranged by size: 

                 895     500      30       3
 
BseYI C'CCAG_C

Cuts at:      0     226     378     470     832     988    1026    1428 
   Size:        226     152      92     362     156      38     402

  Fragments arranged by size: 

                 402     362     226     156     152      92      38
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     233     371     551    1360    1380    1428 
   Size:        233     138     180     809      20      48

  Fragments arranged by size: 

                 809     233     180     138      48      20
 
BsiEI CG_ry'CG

Cuts at:      0    1044    1428 
   Size:       1044     384
 
BsiHKAI G_wGCw'C

Cuts at:      0      56     457    1007    1428 
   Size:         56     401     550     421

  Fragments arranged by size: 

                 550     421     401      56
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     249    1428 
   Size:        249    1179
 
Bsp1286I G_dGCh'C

Cuts at:      0      56     457     776     826    1007    1027    1290    1428 
   Size:         56     401     319      50     181      20     263     138

  Fragments arranged by size: 

                 401     319     263     181     138      56      50      20
            
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     950    1428 
   Size:        950     478
 
BsrBI CCG'CTC

Cuts at:      0    1058    1275    1395    1428 
   Size:       1058     217     120      33

  Fragments arranged by size: 

                1058     217     120      33
 
BsrFI r'CCGG_y

Cuts at:      0     999    1043    1177    1428 
   Size:        999      44     134     251

  Fragments arranged by size: 

                 999     251     134      44
 
BssSI C'ACGA_G

Cuts at:      0    1006    1428 
   Size:       1006     422
 
BstEII G'GTnAC_C

Cuts at:      0     105     837    1428 
   Size:        105     732     591

  Fragments arranged by size: 

                 732     591     105
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     429    1428 
   Size:        429     999
 
BstYI r'GATC_y

Cuts at:      0     213    1118    1253    1428 
   Size:        213     905     135     175

  Fragments arranged by size: 

                 905     213     175     135
 
BtgI C'CryG_G

Cuts at:      0     641    1428 
   Size:        641     787
 
BtsI GCAGTG_nn'

Cuts at:      0     946    1149    1290    1428 
   Size:        946     203     141     138

  Fragments arranged by size: 

                 946     203     141     138
 
EaeI y'GGCC_r

Cuts at:      0     161     431    1209    1428 
   Size:        161     270     778     219

  Fragments arranged by size: 

                 778     270     219     161
 
EarI CTCTTCn'nnn_

Cuts at:      0     196     594     661    1428 
   Size:        196     398      67     767

  Fragments arranged by size: 

                 767     398     196      67
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     488    1400    1428 
   Size:        488     912      28

  Fragments arranged by size: 

                 912     488      28
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     215    1119    1428 
   Size:        215     904     309

  Fragments arranged by size: 

                 904     309     215
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     544    1428 
   Size:        544     884
 
EcoO109I rG'GnC_Cy

Cuts at:      0      87     136     439     549     822     823     871    1013 
   Size:         87      49     303     110     273       1      48     142

Cuts at:   1013    1428 
   Size:        415

  Fragments arranged by size: 

                 415     303     273     142     110      87      49      48
                   1
 
FspI TGC'GCA

Cuts at:      0    1129    1428 
   Size:       1129     299
 
HaeII r_GCGC'y

Cuts at:      0    1245    1428 
   Size:       1245     183
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     190     222    1428 
   Size:        190      32    1206

  Fragments arranged by size: 

                1206     190      32
 
KpnI G_GTAC'C

Cuts at:      0    1355    1428 
   Size:       1355      73
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     191    1096    1428 
   Size:        191     905     332

  Fragments arranged by size: 

                 905     332     191
 
MscI TGG'CCA

Cuts at:      0     433    1428 
   Size:        433     995
 
MslI CAynn'nnrTG

Cuts at:      0     103     187     274     696    1178    1428 
   Size:        103      84      87     422     482     250

  Fragments arranged by size: 

                 482     422     250     103      87      84
 
MspA1I CmG'CkG

Cuts at:      0      60     317     724    1428 
   Size:         60     257     407     704

  Fragments arranged by size: 

                 704     407     257      60
 
NspI r_CATG'y

Cuts at:      0    1128    1428 
   Size:       1128     300
 
PasI CC'CwG_GG

Cuts at:      0     444     816    1428 
   Size:        444     372     612

  Fragments arranged by size: 

                 612     444     372
 
PfoI T'CCnGG_A

Cuts at:      0     678    1428 
   Size:        678     750
 
PpuMI rG'GwC_Cy

Cuts at:      0      87     439     549     871    1428 
   Size:         87     352     110     322     557

  Fragments arranged by size: 

                 557     352     322     110      87
 
PshAI GACnn'nnGTC

Cuts at:      0     650    1428 
   Size:        650     778
 
PspOMI G'GGCC_C

Cuts at:      0     822    1428 
   Size:        822     606
 
PvuII CAG'CTG

Cuts at:      0      60     317     724    1428 
   Size:         60     257     407     704

  Fragments arranged by size: 

                 704     407     257      60
 
SanDI GG'GwC_CC

Cuts at:      0     871    1428 
   Size:        871     557
 
SfcI C'TryA_G

Cuts at:      0    1316    1428 
   Size:       1316     112
 
SgrAI Cr'CCGG_yG

Cuts at:      0     999    1177    1428 
   Size:        999     178     251

  Fragments arranged by size: 

                 999     251     178
 
SphI G_CATG'C

Cuts at:      0    1128    1428 
   Size:       1128     300
 
SspI AAT'ATT

Cuts at:      0     342    1428 
   Size:        342    1086
 
StuI AGG'CCT

Cuts at:      0     966    1428 
   Size:        966     462
 
StyI C'CwwG_G

Cuts at:      0      11    1428 
   Size:         11    1417
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     436    1174    1186    1428 
   Size:        436     738      12     242

  Fragments arranged by size: 

                 738     436     242      12
 
TatI w'GTAC_w

Cuts at:      0     491    1428 
   Size:        491     937
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1064    1096    1269    1301    1428 
   Size:       1064      32     173      32     127

  Fragments arranged by size: 

                1064     173     127      32      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     126     441    1428 
   Size:        126     315     987

  Fragments arranged by size: 

                 987     315     126
 
XmnI GAAnn'nnTTC

Cuts at:      0    1189    1428 
   Size:       1189     239
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AcuI     AgeI     AleI     AlfI     ApaI
    ApaLI     BanI    BanII     BclI     BglI     BlpI Bme1580I    BmgBI
     BmrI     BplI     BpmI     BsaI    BsaHI    BsaWI    BseRI    BseYI
     BsgI    BsiEI  BsiHKAI    BsmBI Bsp1286I    BspMI    BsrBI    BsrFI
    BssSI   BstEII    BstXI    BstYI     BtgI     BtsI     EaeI     EarI
     EciI  Eco57MI    EcoNI EcoO109I     FspI    HaeII    Hin4I     KpnI
     MmeI     MscI     MslI   MspA1I     NspI     PasI     PfoI    PpuMI
    PshAI   PspOMI    PvuII    SanDI     SfcI    SgrAI     SphI     SspI
     StuI     StyI    TaqII     TatI     TstI     XcmI     XmnI

 Enzymes that do not cut: 

    AatII     AclI     AfeI    AflII   AflIII     AhdI     AloI    AlwNI
     ApoI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI
     BbeI     BbsI    BbvCI     BcgI    BciVI    BfrBI    BglII     BmtI
   Bpu10I    BpuEI    BsaAI    BsaBI    BsaXI    BsiWI     BsmI    BspEI
    BspHI    BsrDI    BsrGI   BssHII   BstAPI    BstBI  BstZ17I   Bsu36I
    BtgZI     ClaI    CspCI     DraI   DraIII     DrdI     EagI  EcoICRI
    EcoRI    EcoRV     FalI     FseI    FspAI   HincII  HindIII     HpaI
     KasI     MfeI     MluI     NaeI     NarI     NcoI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     PacI     PciI    PflMI     PmeI
     PmlI     PpiI     PsiI    PspXI     PsrI     PstI     PvuI    RsrII
     SacI    SacII     SalI     SapI     SbfI     ScaI    SexAI     SfiI
     SfoI     SgfI     SmaI     SmlI    SnaBI     SpeI     SrfI     SwaI
  Tth111I     XbaI     XhoI     XmaI     ZraI
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