Restriction map for pF1KB7038
 (Linear) (Six-base) MAPSORT of: KIBB7038.seq  Check: 7238  from: 1  to: 1371



KIBB7038.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 10:58  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1052    1371 
   Size:       1052     319
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     673     808     985    1111    1371 
   Size:        673     135     177     126     260

  Fragments arranged by size: 

                 673     260     177     135     126
 
AfeI AGC'GCT

Cuts at:      0     604     760    1371 
   Size:        604     156     611

  Fragments arranged by size: 

                 611     604     156
 
AflIII A'CryG_T

Cuts at:      0     122     191     722    1371 
   Size:        122      69     531     649

  Fragments arranged by size: 

                 649     531     122      69
 
ApaI G_GGCC'C

Cuts at:      0    1054    1276    1371 
   Size:       1054     222      95

  Fragments arranged by size: 

                1054     222      95
 
AvaI C'yCGr_G

Cuts at:      0      64     162     337     714     859     915    1015    1065 
   Size:         64      98     175     377     145      56     100      50

Cuts at:   1065    1371 
   Size:        306

  Fragments arranged by size: 

                 377     306     175     145     100      98      64      56
                  50
 
BanI G'GyrC_C

Cuts at:      0     253     657     802     898    1005    1371 
   Size:        253     404     145      96     107     366

  Fragments arranged by size: 

                 404     366     253     145     107      96
 
BanII G_rGCy'C

Cuts at:      0      65     184     223     334     403     526     691     840 
   Size:         65     119      39     111      69     123     165     149

Cuts at:    840     994    1054    1276    1371 
   Size:        154      60     222      95

  Fragments arranged by size: 

                 222     165     154     149     123     119     111      95
                  69      65      60      39
 
BbeI G_GCGC'C

Cuts at:      0     257     806    1009    1371 
   Size:        257     549     203     362

  Fragments arranged by size: 

                 549     362     257     203
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     708     742    1371 
   Size:        708      34     629

  Fragments arranged by size: 

                 708     629      34
 
BglI GCCn_nnn'nGGC

Cuts at:      0     286     295     850     877    1371 
   Size:        286       9     555      27     494

  Fragments arranged by size: 

                 555     494     286      27       9
 
BglII A'GATC_T

Cuts at:      0     551    1371 
   Size:        551     820
 
BlpI GC'TnA_GC

Cuts at:      0     544     988    1371 
   Size:        544     444     383

  Fragments arranged by size: 

                 544     444     383
 
Bme1580I G_kGCm'C

Cuts at:      0      29     662     901    1054    1276    1371 
   Size:         29     633     239     153     222      95

  Fragments arranged by size: 

                 633     239     222     153      95      29
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0      90    1371 
   Size:         90    1281
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      63    1371 
   Size:         63    1308
 
Bpu10I CC'TnA_GC

Cuts at:      0     328     424    1371 
   Size:        328      96     947

  Fragments arranged by size: 

                 947     328      96
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     336    1189    1371 
   Size:        336     853     182

  Fragments arranged by size: 

                 853     336     182
 
BsaAI yAC'GTr

Cuts at:      0     125     204    1371 
   Size:        125      79    1167

  Fragments arranged by size: 

                1167     125      79
 
BsaHI Gr'CG_yC

Cuts at:      0     254     803     973    1006    1301    1371 
   Size:        254     549     170      33     295      70

  Fragments arranged by size: 

                 549     295     254     170      70      33
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     376     406     616     646    1111    1141    1371 
   Size:        376      30     210      30     465      30     230

  Fragments arranged by size: 

                 465     376     230     210      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      66    1356    1371 
   Size:         66    1290      15

  Fragments arranged by size: 

                1290      66      15
 
BseYI C'CCAG_C

Cuts at:      0       3     322    1047    1371 
   Size:          3     319     725     324

  Fragments arranged by size: 

                 725     324     319       3
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     688    1371 
   Size:        688     683
 
BsiEI CG_ry'CG

Cuts at:      0     146     281     290     353     830    1371 
   Size:        146     135       9      63     477     541

  Fragments arranged by size: 

                 541     477     146     135      63       9
 
BsiHKAI G_wGCw'C

Cuts at:      0     941    1371 
   Size:        941     430
 
BsiWI C'GTAC_G

Cuts at:      0     200    1371 
   Size:        200    1171
 
Bsp1286I G_dGCh'C

Cuts at:      0      29      65     184     223     334     403     526     662 
   Size:         29      36     119      39     111      69     123     136

Cuts at:    662     691     840     901     941     994    1054    1276    1371 
   Size:         29     149      61      40      53      60     222      95

  Fragments arranged by size: 

                 222     149     136     123     119     111      95      69
                  61      60      53      40      39      36      29      29
            
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     752    1052    1371 
   Size:        752     300     319

  Fragments arranged by size: 

                 752     319     300
 
BsrBI CCG'CTC

Cuts at:      0      10     336     665    1371 
   Size:         10     326     329     706

  Fragments arranged by size: 

                 706     329     326      10
 
BsrFI r'CCGG_y

Cuts at:      0      97     289     587     808     876     975    1002    1371 
   Size:         97     192     298     221      68      99      27     369

  Fragments arranged by size: 

                 369     298     221     192      99      97      68      27
            
 
BssSI C'ACGA_G

Cuts at:      0      28    1371 
   Size:         28    1343
 
BstYI r'GATC_y

Cuts at:      0     551    1371 
   Size:        551     820
 
Bsu36I CC'TnA_GG

Cuts at:      0    1079    1371 
   Size:       1079     292
 
BtgI C'CryG_G

Cuts at:      0     149     257     359     506    1034    1268    1371 
   Size:        149     108     102     147     528     234     103

  Fragments arranged by size: 

                 528     234     149     147     108     103     102
 
EaeI y'GGCC_r

Cuts at:      0     143     278     287     350     509     827    1265    1313 
   Size:        143     135       9      63     159     318     438      48

Cuts at:   1313    1371 
   Size:         58

  Fragments arranged by size: 

                 438     318     159     143     135      63      58      48
                   9
 
EagI C'GGCC_G

Cuts at:      0     143     278     287     350     827    1371 
   Size:        143     135       9      63     477     544

  Fragments arranged by size: 

                 544     477     143     135      63       9
 
EarI CTCTTCn'nnn_

Cuts at:      0     580    1371 
   Size:        580     791
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     610    1371 
   Size:        610     761
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      63     673     808     985    1111    1371 
   Size:         63     610     135     177     126     260

  Fragments arranged by size: 

                 610     260     177     135     126      63
 
EcoO109I rG'GnC_Cy

Cuts at:      0     951    1371 
   Size:        951     420
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     708    1371 
   Size:        708     663
 
FseI GG_CCGG'CC

Cuts at:      0     880    1371 
   Size:        880     491
 
HaeII r_GCGC'y

Cuts at:      0     212     257     606     762     787     806     923     988 
   Size:        212      45     349     156      25      19     117      65

Cuts at:    988    1009    1371 
   Size:         21     362

  Fragments arranged by size: 

                 362     349     212     156     117      65      45      25
                  21      19
 
KasI G'GCGC_C

Cuts at:      0     253     802    1005    1371 
   Size:        253     549     203     366

  Fragments arranged by size: 

                 549     366     253     203
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      62    1371 
   Size:         62    1309
 
MscI TGG'CCA

Cuts at:      0     511    1267    1371 
   Size:        511     756     104

  Fragments arranged by size: 

                 756     511     104
 
MslI CAynn'nnrTG

Cuts at:      0     127     133     190     505    1371 
   Size:        127       6      57     315     866

  Fragments arranged by size: 

                 866     315     127      57       6
 
MspA1I CmG'CkG

Cuts at:      0     158     780     815    1036    1371 
   Size:        158     622      35     221     335

  Fragments arranged by size: 

                 622     335     221     158      35
 
NaeI GCC'GGC

Cuts at:      0      99     291     810     878     977    1004    1371 
   Size:         99     192     519      68      99      27     367

  Fragments arranged by size: 

                 519     367     192      99      99      68      27
 
NarI GG'CG_CC

Cuts at:      0     254     803    1006    1371 
   Size:        254     549     203     365

  Fragments arranged by size: 

                 549     365     254     203
 
NcoI C'CATG_G

Cuts at:      0     149     257     359     506    1268    1371 
   Size:        149     108     102     147     762     103

  Fragments arranged by size: 

                 762     149     147     108     103     102
 
NgoMIV G'CCGG_C

Cuts at:      0      97     289     808     876     975    1002    1371 
   Size:         97     192     519      68      99      27     369

  Fragments arranged by size: 

                 519     369     192      99      97      68      27
 
NotI GC'GGCC_GC

Cuts at:      0     143     827    1371 
   Size:        143     684     544

  Fragments arranged by size: 

                 684     544     143
 
NspI r_CATG'y

Cuts at:      0     138     195     726    1371 
   Size:        138      57     531     645

  Fragments arranged by size: 

                 645     531     138      57
 
PciI A'CATG_T

Cuts at:      0     191     722    1371 
   Size:        191     531     649

  Fragments arranged by size: 

                 649     531     191
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1047    1197    1371 
   Size:       1047     150     174

  Fragments arranged by size: 

                1047     174     150
 
PspOMI G'GGCC_C

Cuts at:      0    1050    1272    1371 
   Size:       1050     222      99

  Fragments arranged by size: 

                1050     222      99
 
PspXI vC'TCGA_Gb

Cuts at:      0     915    1371 
   Size:        915     456
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     724     756    1371 
   Size:        724      32     615

  Fragments arranged by size: 

                 724     615      32
 
PvuII CAG'CTG

Cuts at:      0     780    1371 
   Size:        780     591
 
SacII CC_GC'GG

Cuts at:      0    1037    1371 
   Size:       1037     334
 
SfcI C'TryA_G

Cuts at:      0     450    1371 
   Size:        450     921
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0     286    1371 
   Size:        286    1085
 
SfoI GGC'GCC

Cuts at:      0     255     804    1007    1371 
   Size:        255     549     203     364

  Fragments arranged by size: 

                 549     364     255     203
 
SmaI CCC'GGG

Cuts at:      0    1017    1067    1371 
   Size:       1017      50     304

  Fragments arranged by size: 

                1017     304      50
 
SmlI C'TyrA_G

Cuts at:      0     315     915    1204    1371 
   Size:        315     600     289     167

  Fragments arranged by size: 

                 600     315     289     167
 
StuI AGG'CCT

Cuts at:      0     844    1210    1371 
   Size:        844     366     161

  Fragments arranged by size: 

                 844     366     161
 
StyI C'CwwG_G

Cuts at:      0     149     257     359     506    1268    1371 
   Size:        149     108     102     147     762     103

  Fragments arranged by size: 

                 762     149     147     108     103     102
 
TatI w'GTAC_w

Cuts at:      0    1343    1371 
   Size:       1343      28
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      19      51     616     648    1111    1143    1371 
   Size:         19      32     565      32     463      32     228

  Fragments arranged by size: 

                 565     463     228      32      32      32      19
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     264     366    1371 
   Size:        264     102    1005

  Fragments arranged by size: 

                1005     264     102
 
XhoI C'TCGA_G

Cuts at:      0     915    1371 
   Size:        915     456
 
XmaI C'CCGG_G

Cuts at:      0    1015    1065    1371 
   Size:       1015      50     306

  Fragments arranged by size: 

                1015     306      50
 
XmnI GAAnn'nnTTC

Cuts at:      0     760    1371 
   Size:        760     611
 

 Enzymes that do cut:

     AarI     AcuI     AfeI   AflIII     ApaI     AvaI     BanI    BanII
     BbeI     BcgI     BglI    BglII     BlpI Bme1580I     BplI     BpmI
   Bpu10I    BpuEI    BsaAI    BsaHI    BsaXI    BseRI    BseYI     BsgI
    BsiEI  BsiHKAI    BsiWI Bsp1286I    BspMI    BsrBI    BsrFI    BssSI
    BstYI   Bsu36I     BtgI     EaeI     EagI     EarI     EciI  Eco57MI
 EcoO109I     FalI     FseI    HaeII     KasI     MmeI     MscI     MslI
   MspA1I     NaeI     NarI     NcoI   NgoMIV     NotI     NspI     PciI
    PflMI   PspOMI    PspXI     PsrI    PvuII    SacII     SfcI     SfiI
     SfoI     SmaI     SmlI     StuI     StyI     TatI     TstI     XcmI
     XhoI     XmaI     XmnI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI    AflII     AgeI     AhdI     AleI
     AlfI     AloI    AlwNI    ApaLI     ApoI     AscI     AseI    AsiSI
    AvrII     BaeI    BamHI     BbsI    BbvCI    BciVI     BclI    BfrBI
    BmgBI     BmrI     BmtI     BsaI    BsaBI    BsaWI     BsmI    BsmBI
    BspEI    BspHI    BsrDI    BsrGI   BssHII   BstAPI    BstBI   BstEII
    BstXI  BstZ17I    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII
     DrdI  EcoICRI    EcoNI    EcoRI    EcoRV     FspI    FspAI    Hin4I
   HincII  HindIII     HpaI     KpnI     MfeI     MluI     NdeI     NheI
     NruI     NsiI     PacI     PasI     PfoI     PmeI     PmlI     PpiI
    PpuMI    PshAI     PsiI     PstI     PvuI    RsrII     SacI     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SgfI    SgrAI    SnaBI
     SpeI     SphI     SrfI     SspI     SwaI    TaqII  Tth111I     XbaI
     ZraI
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