Restriction map for pF1KB7033
##################################
# NO CUT ENZYMES (  101 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AflII       AgeI        AhdI        AocI        AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AspI        AvrII       BamHI       BbsI        BbvCI       
BcgI        BciVI       BclI        BfrI        BglII       
BlnI        BpuAI       BsaBI       BsaI        BsaWI       
BseAI       BsiWI       BsmI        Bsp1407I    BspDI       
BspEI       BsrGI       Bst1107I    BstBI       BstEII      
BstPI       BstZ17I     Bsu36I      ClaI        DraI        
DrdI        Eam1105I    Eco81I      EcoNI       EcoO65I     
EcoRV       EcoT22I     FbaI        FseI        HindIII     
HpaI        KpnI        KspI        MamI        MfeI        
MluI        MroI        MseI        MunI        NcoI        
NdeI        NheI        NotI        NruI        NsiI        
NspV        PacI        PaeR7I      PflFI       PinAI       
PmeI        PpuMI       PshAI       PshBI       Psp1406I    
PvuI        SacII       SalI        SbfI        SfuI        
SgrAI       SmaI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        SwaI        Tru9I       
Tth111I     XbaI        XhoI        XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   44 enzymes) #
###################################

AcsI          634  GAATTC               [GA]^AATT[CT]
ApaI         1137  GGGCCC               GGGCC^C
ApoI          634  GAATTC               [GA]^AATT[CT]
AvaI          808  CCCGAG               C^[CT]CG[GA]G
AviII         398  TGCGCA               TGC^GCA
BbeI         1166  GGCGCC               GGCGC^C
BbrPI         334  CACGTG               CAC^GTG
BsiEI         464  CGGCCG               CG[GA][CT]^CG
BsmAI         621  GAGAC                GTCTC(1/5)
BsmBI         621  GAGACG               CGTCTC(1/5)
BsoBI         808  CCCGAG               C^[CT]CG[GA]G
BspHI        1067  TCATGA               T^CATGA
BsrDI        1101  CATTGC               GCAATG(2/0)
BssHII        891  GCGCGC               G^CGCGC
BssSI        1075  CACGAG               CACGAG(-5/-1)
CpoI          548  CGGACCG              CG^G[AT]CCG
EagI          464  CGGCCG               C^GGCCG
EarI          182  CTCTTC               CTCTTC(1/4)
EclXI         464  CGGCCG               C^GGCCG
Eco52I        464  CGGCCG               C^GGCCG
EcoRI         634  GAATTC               G^AATTC
EcoT14I       425  CCAAGG               C^C[AT][AT]GG
FspI          398  TGCGCA               TGC^GCA
HincII       1045  GTCAAC               GT[CT]^[GA]AC
HindII       1045  GTCAAC               GT[CT]^[GA]AC
KasI         1166  GGCGCC               G^GCGCC
Ksp632I       182  CTCTTC               CTCTTC(1/4)
NaeI          462  GCCGGC               GCC^GGC
NarI         1166  GGCGCC               GG^CGCC
NgoMIV        462  GCCGGC               G^CCGGC
PmaCI         334  CACGTG               CAC^GTG
PmlI          334  CACGTG               CAC^GTG
RcaI         1067  TCATGA               T^CATGA
RsrII         548  CGGACCG              CG^G[AT]CCG
SacI          598  GAGCTC               GAGCT^C
SapI          181  GCTCTTC              GCTCTTC(1/4)
ScaI          839  AGTACT               AGT^ACT
SfiI          114  GGCCTTATCGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI         1166  GGCGCC               GGC^GCC
StyI          425  CCAAGG               C^C[AT][AT]GG
TaqI          929  TCGA                 T^CGA
TfiI          198  GATTC                G^A[AT]TC
TthHB8I       929  TCGA                 T^CGA
XcmI         1148  CCACTGCCGCCCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   36 enzymes) #
###################################

AcyI          623  GACGCC               G[GA]^CG[CT]C
             1166  GGCGCC              
AfaI          747  GTAC                 GT^AC
              840  GTAC                
AflIII        294  ACATGT               A^C[GA][CT]GT
              908  ACATGT              
Alw44I        298  GTGCAC               G^TGCAC
              537  GTGCAC              
AlwI          816  GATCC                GGATC(4/5)
              369  GGATC               
Aor51HI       902  AGCGCT               AGC^GCT
              968  AGCGCT              
ApaLI         298  GTGCAC               G^TGCAC
              537  GTGCAC              
BanII         598  GAGCTC               G[GA]GC[CT]^C
             1137  GGGCCC              
BfaI          451  CTAG                 C^TAG
              964  CTAG                
BsaHI         623  GACGCC               G[GA]^CG[CT]C
             1166  GGCGCC              
BspLU11I      294  ACATGT               A^CATGT
              908  ACATGT              
BsrBI          12  GAGCGG               CCGCTC(-3/-3)
              582  GAGCGG              
BsrFI         462  GCCGGC               [GA]^CCGG[CT]
              466  GCCGGT              
BstF5I        410  CATCC                GGATG(2/0)
              660  CATCC               
BstYI         369  GGATCT               [GA]^GATC[CT]
              815  AGATCC              
Cfr10I        462  GCCGGC               [GA]^CCGG[CT]
              466  GCCGGT              
DraII         764  AGGCCCT              [GA]G^G[ACGT]CC[CT]
             1136  AGGGCCC             
DsaI           65  CCACGG               C^C[GA][CT]GG
               77  CCACGG              
Eco47III      902  AGCGCT               AGC^GCT
              968  AGCGCT              
EcoO109I      764  AGGCCCT              [GA]G^G[ACGT]CC[CT]
             1136  AGGGCCC             
FokI          410  CATCC                GGATG(9/13)
              660  CATCC               
Hin1I         623  GACGCC               G[GA]^CG[CT]C
             1166  GGCGCC              
MaeI          451  CTAG                 C^TAG
              964  CTAG                
MboII         183  TCTTC                GAAGA(8/7)
              923  TCTTC               
MflI          369  GGATCT               [GA]^GATC[CT]
              815  AGATCC              
NspI          294  ACATGT               [GA]CATG^[CT]
              908  ACATGT              
PflMI         846  CCACCTCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1086  CCAGACGCTGG         
PstI          898  CTGCAG               CTGCA^G
             1117  CTGCAG              
RsaI          747  GTAC                 GT^AC
              840  GTAC                
SexAI         377  ACCTGGT              A^CC[AT]GGT
              941  ACCTGGT             
SfaNI        1000  GATGC                GCATC(5/9)
              409  GCATC               
SfcI          898  CTGCAG               C^T[GA][CT]AG
             1117  CTGCAG              
StuI          113  AGGCCT               AGG^CCT
              713  AGGCCT              
Tsp509I       635  AATT                 ^AATT
             1207  AATT                
Van91I        846  CCACCTCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1086  CCAGACGCTGG         
XhoII         369  GGATCT               [GA]^GATC[CT]
              815  AGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   77 enzymes) #
#########################################

AccII       AciI        AluI        AlwNI       AspHI       
AvaII       BalI        BanI        BbvI        BcnI        
BglI        BlpI        BmyI        BpmI        Bpu1102I    
BsaAI       BsaJI       BseRI       BsgI        BsiHKAI     
BsiYI       BslI        BsmFI       Bsp1286I    BspMI       
BsrI        BssKI       BstAPI      BstNI       BstUI       
BstXI       Cac8I       CelII       CfoI        Cfr13I      
DdeI        DpnII       DraIII      EaeI        EcoRII      
FauI        Fnu4HI      HaeII       HaeIII      HapII       
HgaI        HhaI        HinP1I      HinfI       HpaII       
HphI        ItaI        MaeII       MaeIII      MboI        
MluNI       MnlI        MscI        MslI        MspA1I      
MspI        MvaI        MvnI        MwoI        NciI        
NdeII       NlaIII      NlaIV       PleI        PvuII       
Sau3AI      Sau96I      ScrFI       SmlI        TseI        
Tsp45I      TspRI       
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