Restriction map for pF1KB7033
 (Linear) (Six-base) MAPSORT of: KIBB7033.seq  Check: 8389  from: 1  to: 1272



KIBB7033.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 10:58  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     249     785    1272 
   Size:        249     536     487

  Fragments arranged by size: 

                 536     487     249
 
AfeI AGC'GCT

Cuts at:      0     904     970    1272 
   Size:        904      66     302

  Fragments arranged by size: 

                 904     302      66
 
AflIII A'CryG_T

Cuts at:      0     294     908    1272 
   Size:        294     614     364

  Fragments arranged by size: 

                 614     364     294
 
AleI CACnn'nnGTG

Cuts at:      0      70     143     886     989    1272 
   Size:         70      73     743     103     283

  Fragments arranged by size: 

                 743     283     103      73      70
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     904    1272 
   Size:        904     368
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     367     399    1272 
   Size:        367      32     873

  Fragments arranged by size: 

                 873     367      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     525    1092    1125    1180    1201    1272 
   Size:        525     567      33      55      21      71

  Fragments arranged by size: 

                 567     525      71      55      33      21
 
ApaI G_GGCC'C

Cuts at:      0    1141    1272 
   Size:       1141     131
 
ApaLI G'TGCA_C

Cuts at:      0     298     537    1272 
   Size:        298     239     735

  Fragments arranged by size: 

                 735     298     239
 
ApoI r'AATT_y

Cuts at:      0     634    1272 
   Size:        634     638
 
AvaI C'yCGr_G

Cuts at:      0     808    1272 
   Size:        808     464
 
BanI G'GyrC_C

Cuts at:      0     137     782    1166    1272 
   Size:        137     645     384     106

  Fragments arranged by size: 

                 645     384     137     106
 
BanII G_rGCy'C

Cuts at:      0     602    1141    1272 
   Size:        602     539     131

  Fragments arranged by size: 

                 602     539     131
 
BbeI G_GCGC'C

Cuts at:      0    1170    1272 
   Size:       1170     102
 
BglI GCCn_nnn'nGGC

Cuts at:      0     121    1111    1159    1175    1272 
   Size:        121     990      48      16      97

  Fragments arranged by size: 

                 990     121      97      48      16
 
BlpI GC'TnA_GC

Cuts at:      0      20     151     232    1130    1272 
   Size:         20     131      81     898     142

  Fragments arranged by size: 

                 898     142     131      81      20
 
Bme1580I G_kGCm'C

Cuts at:      0     302     541    1141    1272 
   Size:        302     239     600     131

  Fragments arranged by size: 

                 600     302     239     131
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     298     576     790     997    1157    1272 
   Size:        298     278     214     207     160     115

  Fragments arranged by size: 

                 298     278     214     207     160     115
 
Bpu10I CC'TnA_GC

Cuts at:      0      44    1272 
   Size:         44    1228
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     487     586     820    1272 
   Size:        487      99     234     452

  Fragments arranged by size: 

                 487     452     234      99
 
BsaAI yAC'GTr

Cuts at:      0     336     516    1215    1272 
   Size:        336     180     699      57

  Fragments arranged by size: 

                 699     336     180      57
 
BsaHI Gr'CG_yC

Cuts at:      0     624    1167    1272 
   Size:        624     543     105

  Fragments arranged by size: 

                 624     543     105
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     589     619     898     928    1272 
   Size:        589      30     279      30     344

  Fragments arranged by size: 

                 589     344     279      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     295     823     826    1272 
   Size:        295     528       3     446

  Fragments arranged by size: 

                 528     446     295       3
 
BseYI C'CCAG_C

Cuts at:      0     877    1140    1272 
   Size:        877     263     132

  Fragments arranged by size: 

                 877     263     132
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     259     277     325     538    1018    1272 
   Size:        259      18      48     213     480     254

  Fragments arranged by size: 

                 480     259     254     213      48      18
 
BsiEI CG_ry'CG

Cuts at:      0     467    1272 
   Size:        467     805
 
BsiHKAI G_wGCw'C

Cuts at:      0     302     541     602     694     776     916    1057    1272 
   Size:        302     239      61      92      82     140     141     215

  Fragments arranged by size: 

                 302     239     215     141     140      92      82      61
            
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     615    1272 
   Size:        615     657
 
Bsp1286I G_dGCh'C

Cuts at:      0     302     541     602     694     776     916    1057    1141 
   Size:        302     239      61      92      82     140     141      84

Cuts at:   1141    1272 
   Size:        131

  Fragments arranged by size: 

                 302     239     141     140     131      92      84      82
                  61
 
BspHI T'CATG_A

Cuts at:      0    1067    1272 
   Size:       1067     205
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     249     404     785    1272 
   Size:        249     155     381     487

  Fragments arranged by size: 

                 487     381     249     155
 
BsrBI CCG'CTC

Cuts at:      0      14     584    1272 
   Size:         14     570     688

  Fragments arranged by size: 

                 688     570      14
 
BsrDI GCAATG_nn'

Cuts at:      0    1100    1272 
   Size:       1100     172
 
BsrFI r'CCGG_y

Cuts at:      0     462     466    1272 
   Size:        462       4     806

  Fragments arranged by size: 

                 806     462       4
 
BssHII G'CGCG_C

Cuts at:      0     891    1272 
   Size:        891     381
 
BssSI C'ACGA_G

Cuts at:      0    1075    1272 
   Size:       1075     197
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     255     306    1061    1272 
   Size:        255      51     755     211

  Fragments arranged by size: 

                 755     255     211      51
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0      72     432     991    1272 
   Size:         72     360     559     281

  Fragments arranged by size: 

                 559     360     281      72
 
BstYI r'GATC_y

Cuts at:      0     369     815    1272 
   Size:        369     446     457

  Fragments arranged by size: 

                 457     446     369
 
BtgI C'CryG_G

Cuts at:      0      65      77    1272 
   Size:         65      12    1195

  Fragments arranged by size: 

                1195      65      12
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     777    1272 
   Size:        777     495
 
BtsI GCAGTG_nn'

Cuts at:      0     408     437    1030    1148    1272 
   Size:        408      29     593     118     124

  Fragments arranged by size: 

                 593     408     124     118      29
 
DraIII CAC_nnn'GTG

Cuts at:      0     306    1041    1061    1272 
   Size:        306     735      20     211

  Fragments arranged by size: 

                 735     306     211      20
 
EaeI y'GGCC_r

Cuts at:      0      62      74     157     464     684    1272 
   Size:         62      12      83     307     220     588

  Fragments arranged by size: 

                 588     307     220      83      62      12
 
EagI C'GGCC_G

Cuts at:      0     464    1272 
   Size:        464     808
 
EarI CTCTTCn'nnn_

Cuts at:      0     188    1272 
   Size:        188    1084
 
EcoICRI GAG'CTC

Cuts at:      0     600    1272 
   Size:        600     672
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     298     576     790     997    1157    1272 
   Size:        298     278     214     207     160     115

  Fragments arranged by size: 

                 298     278     214     207     160     115
 
EcoO109I rG'GnC_Cy

Cuts at:      0     765    1137    1272 
   Size:        765     372     135

  Fragments arranged by size: 

                 765     372     135
 
EcoRI G'AATT_C

Cuts at:      0     634    1272 
   Size:        634     638
 
FspI TGC'GCA

Cuts at:      0     400    1272 
   Size:        400     872
 
HaeII r_GCGC'y

Cuts at:      0     906     972    1083    1117    1170    1272 
   Size:        906      66     111      34      53     102

  Fragments arranged by size: 

                 906     111     102      66      53      34
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     103     135    1272 
   Size:        103      32    1137

  Fragments arranged by size: 

                1137     103      32
 
HincII GTy'rAC

Cuts at:      0    1047    1272 
   Size:       1047     225
 
KasI G'GCGC_C

Cuts at:      0    1166    1272 
   Size:       1166     106
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     599    1272 
   Size:        599     673
 
MscI TGG'CCA

Cuts at:      0      76     159     686    1272 
   Size:         76      83     527     586

  Fragments arranged by size: 

                 586     527      83      76
 
MslI CAynn'nnrTG

Cuts at:      0      70     143     703     886     989    1272 
   Size:         70      73     560     183     103     283

  Fragments arranged by size: 

                 560     283     183     103      73      70
 
MspA1I CmG'CkG

Cuts at:      0     325     420     554     954    1144    1272 
   Size:        325      95     134     400     190     128

  Fragments arranged by size: 

                 400     325     190     134     128      95
 
NaeI GCC'GGC

Cuts at:      0     464    1272 
   Size:        464     808
 
NarI GG'CG_CC

Cuts at:      0    1167    1272 
   Size:       1167     105
 
NgoMIV G'CCGG_C

Cuts at:      0     462    1272 
   Size:        462     810
 
NspI r_CATG'y

Cuts at:      0     298     912    1272 
   Size:        298     614     360

  Fragments arranged by size: 

                 614     360     298
 
PciI A'CATG_T

Cuts at:      0     294     908    1272 
   Size:        294     614     364

  Fragments arranged by size: 

                 614     364     294
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     852    1092    1272 
   Size:        852     240     180

  Fragments arranged by size: 

                 852     240     180
 
PfoI T'CCnGG_A

Cuts at:      0     662    1272 
   Size:        662     610
 
PmlI CAC'GTG

Cuts at:      0     336    1272 
   Size:        336     936
 
PspOMI G'GGCC_C

Cuts at:      0    1137    1272 
   Size:       1137     135
 
PstI C_TGCA'G

Cuts at:      0     902    1121    1272 
   Size:        902     219     151

  Fragments arranged by size: 

                 902     219     151
 
PvuII CAG'CTG

Cuts at:      0     325     420    1144    1272 
   Size:        325      95     724     128

  Fragments arranged by size: 

                 724     325     128      95
 
RsrII CG'GwC_CG

Cuts at:      0     549    1272 
   Size:        549     723
 
SacI G_AGCT'C

Cuts at:      0     602    1272 
   Size:        602     670
 
SapI GCTCTTCn'nnn_

Cuts at:      0     188    1272 
   Size:        188    1084
 
ScaI AGT'ACT

Cuts at:      0     841    1272 
   Size:        841     431
 
SexAI A'CCwGG_T

Cuts at:      0     377     941    1272 
   Size:        377     564     331

  Fragments arranged by size: 

                 564     377     331
 
SfcI C'TryA_G

Cuts at:      0     898    1117    1272 
   Size:        898     219     155

  Fragments arranged by size: 

                 898     219     155
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0     121    1272 
   Size:        121    1151
 
SfoI GGC'GCC

Cuts at:      0    1168    1272 
   Size:       1168     104
 
SmlI C'TyrA_G

Cuts at:      0     502     601     835    1272 
   Size:        502      99     234     437

  Fragments arranged by size: 

                 502     437     234      99
 
StuI AGG'CCT

Cuts at:      0     115     715    1272 
   Size:        115     600     557

  Fragments arranged by size: 

                 600     557     115
 
StyI C'CwwG_G

Cuts at:      0     425    1272 
   Size:        425     847
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     737    1272 
   Size:        737     535
 
TatI w'GTAC_w

Cuts at:      0     839    1272 
   Size:        839     433
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     281     313     789     821    1272 
   Size:        281      32     476      32     451

  Fragments arranged by size: 

                 476     451     281      32      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1155    1272 
   Size:       1155     117
 

 Enzymes that do cut:

     AarI     AfeI   AflIII     AleI     AlfI     AloI    AlwNI     ApaI
    ApaLI     ApoI     AvaI     BanI    BanII     BbeI     BglI     BlpI
 Bme1580I     BpmI   Bpu10I    BpuEI    BsaAI    BsaHI    BsaXI    BseRI
    BseYI     BsgI    BsiEI  BsiHKAI    BsmBI Bsp1286I    BspHI    BspMI
    BsrBI    BsrDI    BsrFI   BssHII    BssSI   BstAPI    BstXI    BstYI
     BtgI    BtgZI     BtsI   DraIII     EaeI     EagI     EarI  EcoICRI
  Eco57MI EcoO109I    EcoRI     FspI    HaeII    Hin4I   HincII     KasI
     MmeI     MscI     MslI   MspA1I     NaeI     NarI   NgoMIV     NspI
     PciI    PflMI     PfoI     PmlI   PspOMI     PstI    PvuII    RsrII
     SacI     SapI     ScaI    SexAI     SfcI     SfiI     SfoI     SmlI
     StuI     StyI    TaqII     TatI     TstI     XcmI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AcuI    AflII     AgeI     AhdI
     AscI     AseI    AsiSI    AvrII     BaeI    BamHI     BbsI    BbvCI
     BcgI    BciVI     BclI    BfrBI    BglII    BmgBI     BmrI     BmtI
     BplI     BsaI    BsaBI    BsaWI    BsiWI     BsmI    BspEI    BsrGI
    BstBI   BstEII  BstZ17I   Bsu36I     ClaI    CspCI     DraI     DrdI
     EciI    EcoNI    EcoRV     FalI     FseI    FspAI  HindIII     HpaI
     KpnI     MfeI     MluI     NcoI     NdeI     NheI     NotI     NruI
     NsiI     PacI     PasI     PmeI     PpiI    PpuMI    PshAI     PsiI
    PspXI     PsrI     PvuI    SacII     SalI    SanDI     SbfI     SgfI
    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     SwaI
  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI
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