Restriction map for pF1KB7032
 (Linear) (Six-base) MAPSORT of: KIBB7032.seq  Check: 1406  from: 1  to: 1272



KIBB7032.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 10:58  ..

Acc65I G'GTAC_C

Cuts at:      0    1218    1272 
   Size:       1218      54
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     728    1272 
   Size:        728     544
 
AleI CACnn'nnGTG

Cuts at:      0     190    1272 
   Size:        190    1082
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     268    1272 
   Size:        268    1004
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1192    1224    1272 
   Size:       1192      32      48

  Fragments arranged by size: 

                1192      48      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     847    1272 
   Size:        847     425
 
ApoI r'AATT_y

Cuts at:      0    1021    1131    1272 
   Size:       1021     110     141

  Fragments arranged by size: 

                1021     141     110
 
AvaI C'yCGr_G

Cuts at:      0    1064    1272 
   Size:       1064     208
 
BanI G'GyrC_C

Cuts at:      0     206    1218    1272 
   Size:        206    1012      54

  Fragments arranged by size: 

                1012     206      54
 
BbeI G_GCGC'C

Cuts at:      0     210    1272 
   Size:        210    1062
 
BbvCI CC'TCA_GC

Cuts at:      0     944    1272 
   Size:        944     328
 
BclI T'GATC_A

Cuts at:      0     500     656     794    1272 
   Size:        500     156     138     478

  Fragments arranged by size: 

                 500     478     156     138
 
Bme1580I G_kGCm'C

Cuts at:      0    1065    1272 
   Size:       1065     207
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     106    1272 
   Size:        106    1166
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     466    1226    1272 
   Size:        466     760      46

  Fragments arranged by size: 

                 760     466      46
 
Bpu10I CC'TnA_GC

Cuts at:      0     545     944    1272 
   Size:        545     399     328

  Fragments arranged by size: 

                 545     399     328
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      77    1272 
   Size:         77    1195
 
BsaAI yAC'GTr

Cuts at:      0     807    1272 
   Size:        807     465
 
BsaHI Gr'CG_yC

Cuts at:      0     207    1272 
   Size:        207    1065
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      43     961    1191    1272 
   Size:         43     918     230      81

  Fragments arranged by size: 

                 918     230      81      43
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1077    1272 
   Size:       1077     195
 
BsmI GAATG_Cn'

Cuts at:      0     457    1272 
   Size:        457     815
 
Bsp1286I G_dGCh'C

Cuts at:      0    1065    1272 
   Size:       1065     207
 
BspHI T'CATG_A

Cuts at:      0     533    1272 
   Size:        533     739
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      71    1272 
   Size:         71    1201
 
BsrBI CCG'CTC

Cuts at:      0     119    1272 
   Size:        119    1153
 
BsrDI GCAATG_nn'

Cuts at:      0     938    1272 
   Size:        938     334
 
BssHII G'CGCG_C

Cuts at:      0      43    1272 
   Size:         43    1229
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     617    1272 
   Size:        617     655
 
BstEII G'GTnAC_C

Cuts at:      0     637    1272 
   Size:        637     635
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     192    1272 
   Size:        192    1080
 
BstYI r'GATC_y

Cuts at:      0     950    1272 
   Size:        950     322
 
Bsu36I CC'TnA_GG

Cuts at:      0     643    1272 
   Size:        643     629
 
BtgI C'CryG_G

Cuts at:      0     291    1051    1272 
   Size:        291     760     221

  Fragments arranged by size: 

                 760     291     221
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0      33      93    1272 
   Size:         33      60    1179

  Fragments arranged by size: 

                1179      60      33
 
BtsI GCAGTG_nn'

Cuts at:      0    1065    1272 
   Size:       1065     207
 
DraIII CAC_nnn'GTG

Cuts at:      0     356    1272 
   Size:        356     916
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     871    1272 
   Size:        871     401
 
EaeI y'GGCC_r

Cuts at:      0       5     461     854    1272 
   Size:          5     456     393     418

  Fragments arranged by size: 

                 456     418     393       5
 
EarI CTCTTCn'nnn_

Cuts at:      0     262     400    1272 
   Size:        262     138     872

  Fragments arranged by size: 

                 872     262     138
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     466     728    1226    1272 
   Size:        466     262     498      46

  Fragments arranged by size: 

                 498     466     262      46
 
EcoO109I rG'GnC_Cy

Cuts at:      0     122    1272 
   Size:        122    1150
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     636    1272 
   Size:        636     636
 
FspI TGC'GCA

Cuts at:      0      67     676    1272 
   Size:         67     609     596

  Fragments arranged by size: 

                 609     596      67
 
FspAI rTGC'GCAy

Cuts at:      0     676    1272 
   Size:        676     596
 
HaeII r_GCGC'y

Cuts at:      0     210    1272 
   Size:        210    1062
 
KasI G'GCGC_C

Cuts at:      0     206    1272 
   Size:        206    1066
 
KpnI G_GTAC'C

Cuts at:      0    1222    1272 
   Size:       1222      50
 
MscI TGG'CCA

Cuts at:      0       7     463     856    1272 
   Size:          7     456     393     416

  Fragments arranged by size: 

                 456     416     393       7
 
MslI CAynn'nnrTG

Cuts at:      0     190     532     682    1272 
   Size:        190     342     150     590

  Fragments arranged by size: 

                 590     342     190     150
 
MspA1I CmG'CkG

Cuts at:      0     248    1272 
   Size:        248    1024
 
NarI GG'CG_CC

Cuts at:      0     207    1272 
   Size:        207    1065
 
NcoI C'CATG_G

Cuts at:      0     291    1272 
   Size:        291     981
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     216     867    1272 
   Size:        216     651     405

  Fragments arranged by size: 

                 651     405     216
 
PfoI T'CCnGG_A

Cuts at:      0      23    1272 
   Size:         23    1249
 
PmlI CAC'GTG

Cuts at:      0     807    1272 
   Size:        807     465
 
PshAI GACnn'nnGTC

Cuts at:      0     367    1272 
   Size:        367     905
 
PstI C_TGCA'G

Cuts at:      0     247    1272 
   Size:        247    1025
 
PvuII CAG'CTG

Cuts at:      0     248    1272 
   Size:        248    1024
 
SapI GCTCTTCn'nnn_

Cuts at:      0     262    1272 
   Size:        262    1010
 
SfcI C'TryA_G

Cuts at:      0     169     243    1272 
   Size:        169      74    1029

  Fragments arranged by size: 

                1029     169      74
 
SfoI GGC'GCC

Cuts at:      0     208    1272 
   Size:        208    1064
 
SspI AAT'ATT

Cuts at:      0     489    1272 
   Size:        489     783
 
StyI C'CwwG_G

Cuts at:      0     188     291    1272 
   Size:        188     103     981

  Fragments arranged by size: 

                 981     188     103
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     625    1272 
   Size:        625     647
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     864    1272 
   Size:        864     408
 
XmnI GAAnn'nnTTC

Cuts at:      0    1167    1272 
   Size:       1167     105
 

 Enzymes that do cut:

   Acc65I     AcuI     AleI     AlfI     AloI    AlwNI     ApoI     AvaI
     BanI     BbeI    BbvCI     BclI Bme1580I     BplI     BpmI   Bpu10I
     BsaI    BsaAI    BsaHI    BseRI     BsgI     BsmI Bsp1286I    BspHI
    BspMI    BsrBI    BsrDI   BssHII   BstAPI   BstEII    BstXI    BstYI
   Bsu36I     BtgI    BtgZI     BtsI   DraIII     DrdI     EaeI     EarI
  Eco57MI EcoO109I     FalI     FspI    FspAI    HaeII     KasI     KpnI
     MscI     MslI   MspA1I     NarI     NcoI    PflMI     PfoI     PmlI
    PshAI     PstI    PvuII     SapI     SfcI     SfoI     SspI     StyI
    TaqII     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     ApaI    ApaLI     AscI     AseI    AsiSI    AvrII     BaeI
    BamHI    BanII     BbsI     BcgI    BciVI    BfrBI     BglI    BglII
     BlpI    BmgBI     BmrI     BmtI    BpuEI    BsaBI    BsaWI    BsaXI
    BseYI    BsiEI  BsiHKAI    BsiWI    BsmBI    BspEI    BsrFI    BsrGI
    BssSI    BstBI  BstZ17I     ClaI    CspCI     DraI     EagI     EciI
  EcoICRI    EcoNI    EcoRI    EcoRV     FseI    Hin4I   HincII  HindIII
     HpaI     MfeI     MluI     MmeI     NaeI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     NspI     PacI     PasI     PciI     PmeI
     PpiI    PpuMI     PsiI   PspOMI    PspXI     PsrI     PvuI    RsrII
     SacI    SacII     SalI    SanDI     SbfI     ScaI    SexAI     SfiI
     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI
     StuI     SwaI     TatI     TstI  Tth111I     XbaI     XhoI     XmaI
     ZraI
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