Restriction map for pF1KB6006
##################################
# NO CUT ENZYMES (   71 enzymes) #
##################################

AatII       AccII       AccIII      AclI        AflII       
AgeI        Alw44I      Aor51HI     ApaI        ApaLI       
AscI        AvrII       BbeI        BciVI       BfrI        
BlnI        BlpI        Bpu1102I    BseAI       BsiWI       
Bsp1407I    BspDI       BspEI       BspLU11I    BsrGI       
BssHII      Bst1107I    BstBI       BstUI       BstZ17I     
CelII       ClaI        CpoI        Eco47III    FseI        
HindIII     HpaI        KasI        KspI        MfeI        
MluI        MroI        MunI        MvnI        NaeI        
NarI        NgoMIV      NheI        NotI        NruI        
NspV        PacI        PinAI       PmeI        Psp1406I    
PvuI        RsrII       SacII       SbfI        ScaI        
SfiI        SfoI        SfuI        SgrAI       SmaI        
SnaBI       Sse8387I    SspBI       SwaI        XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   28 enzymes) #
###################################

AseI         6304  ATTAAT               AT^TAAT
AsnI         6304  ATTAAT               AT^TAAT
AviII        1670  TGCGCA               TGC^GCA
BamHI        4213  GGATCC               G^GATCC
BsaBI        5140  GATAATGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI         439  ACCGGA               [AT]^CCGG[AT]
BsrBI        3544  CCGCTC               CCGCTC(-3/-3)
BsrDI        5963  GCAATG               GCAATG(2/0)
BsrFI        2791  GCCGGT               [GA]^CCGG[CT]
BstAPI       1432  GCAAACCCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII       3900  GGTGACC              G^GT[ACGT]ACC
BstPI        3900  GGTGACC              G^GT[ACGT]ACC
Cfr10I       2791  GCCGGT               [GA]^CCGG[CT]
DraIII       5591  CACCTGGTG            CAC[ACGT][ACGT][ACGT]^GTG
EagI         6518  CGGCCG               C^GGCCG
EclXI        6518  CGGCCG               C^GGCCG
Eco52I       6518  CGGCCG               C^GGCCG
EcoO65I      3900  GGTGACC              G^GT[ACGT]ACC
EcoT22I      2948  ATGCAT               ATGCA^T
FspI         1670  TGCGCA               TGC^GCA
HaeII        2893  AGCGCC               [GA]GCGC^[CT]
MamI         5140  GATAATGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NsiI         2948  ATGCAT               ATGCA^T
PflMI        1236  CCAAAAAATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI        6304  ATTAAT               AT^TAAT
SpeI         4351  ACTAGT               A^CTAGT
SspI          631  AATATT               AAT^ATT
Van91I       1236  CCAAAAAATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG


###################################
# TWO CUT ENZYMES (   36 enzymes) #
###################################

Acc65I       1327  GGTACC               G^GTACC
             7295  GGTACC              
AcyI           63  GGCGTC               G[GA]^CG[CT]C
             3843  GACGCC              
AflIII       2880  ACACGT               A^C[GA][CT]GT
             4622  ACACGT              
AhdI         4156  GACTCAGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             5662  GACTATGAGTC         
Asp718I      1327  GGTACC               G^GTACC
             7295  GGTACC              
AspEI        4156  GACTCAGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             5662  GACTATGAGTC         
AvaI          991  CTCGAG               C^[CT]CG[GA]G
             3416  CTCGAG              
BbrPI        1717  CACGTG               CAC^GTG
             6932  CACGTG              
BcgI         3147  CGACAAGGCTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
             6982  GCATCCAAGTCG        
BclI          335  TGATCA               T^GATCA
              380  TGATCA              
BsaHI          63  GGCGTC               G[GA]^CG[CT]C
             3843  GACGCC              
BsgI         6929  CTGCAC               GTGCAG(16/14)
             5018  GTGCAG              
BsiEI           5  CGACCG               CG[GA][CT]^CG
             6518  CGGCCG              
BsmBI        3419  GAGACG               CGTCTC(1/5)
             3451  GAGACG              
BsoBI         991  CTCGAG               C^[CT]CG[GA]G
             3416  CTCGAG              
BspHI        3255  TCATGA               T^CATGA
             7269  TCATGA              
DraI         2262  TTTAAA               TTT^AAA
             4537  TTTAAA              
Eam1105I     4156  GACTCAGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             5662  GACTATGAGTC         
EcoRV        3375  GATATC               GAT^ATC
             5347  GATATC              
FauI         4200  CCCGC                CCCGC(4/6)
             6823  GCGGG               
FbaI          335  TGATCA               T^GATCA
              380  TGATCA              
Hin1I          63  GGCGTC               G[GA]^CG[CT]C
             3843  GACGCC              
HincII       3145  GTCGAC               GT[CT]^[GA]AC
             4988  GTCGAC              
HindII       3145  GTCGAC               GT[CT]^[GA]AC
             4988  GTCGAC              
KpnI         1327  GGTACC               GGTAC^C
             7295  GGTACC              
NdeI         4633  CATATG               CA^TATG
             5867  CATATG              
PaeR7I        991  CTCGAG               C^TCGAG
             3416  CTCGAG              
PmaCI        1717  CACGTG               CAC^GTG
             6932  CACGTG              
PmlI         1717  CACGTG               CAC^GTG
             6932  CACGTG              
RcaI         3255  TCATGA               T^CATGA
             7269  TCATGA              
SalI         3145  GTCGAC               G^TCGAC
             4988  GTCGAC              
SapI         1831  GAAGAGC              GCTCTTC(1/4)
             3762  GAAGAGC             
SexAI        5592  ACCTGGT              A^CC[AT]GGT
             5635  ACCAGGT             
SphI         4722  GCATGC               GCATG^C
             7387  GCATGC              
XbaI         1521  TCTAGA               T^CTAGA
             5938  TCTAGA              
XhoI          991  CTCGAG               C^TCGAG
             3416  CTCGAG              


#########################################
# 3 OR MORE CUT ENZYMES (  123 enzymes) #
#########################################

AccI        AciI        AcsI        AfaI        AluI        
AlwI        AlwNI       AocI        ApoI        Asp700I     
AspHI       AspI        AvaII       BalI        BanI        
BanII       BbsI        BbvCI       BbvI        BcnI        
BfaI        BglI        BglII       BmyI        BpmI        
BpuAI       BsaAI       BsaI        BsaJI       BseRI       
BsiHKAI     BsiYI       BslI        BsmAI       BsmFI       
BsmI        Bsp1286I    BspMI       BsrI        BssKI       
BssSI       BstF5I      BstNI       BstXI       BstYI       
Bsu36I      Cac8I       CfoI        Cfr13I      DdeI        
DpnII       DraII       DrdI        DsaI        EaeI        
EarI        Eco81I      EcoNI       EcoO109I    EcoRI       
EcoRII      EcoT14I     Fnu4HI      FokI        HaeIII      
HapII       HgaI        HhaI        HinP1I      HinfI       
HpaII       HphI        ItaI        Ksp632I     MaeI        
MaeII       MaeIII      MboI        MboII       MflI        
MluNI       MnlI        MscI        MseI        MslI        
MspA1I      MspI        MvaI        MwoI        NciI        
NcoI        NdeII       NlaIII      NlaIV       NspI        
PflFI       PleI        PpuMI       PshAI       PstI        
PvuII       RsaI        SacI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        SmlI        StuI        
StyI        TaqI        TfiI        Tru9I       TseI        
Tsp45I      Tsp509I     TspRI       Tth111I     TthHB8I     
XcmI        XhoII       XmnI        
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