Restriction map for pF1KB6006
 (Linear) (Six-base) MAPSORT of: KIBB6006.seq  Check: 8927  from: 1  to: 7404



KIBB6006.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 10:58  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     161    7239    7404 
   Size:        161    7078     165

  Fragments arranged by size: 

                7078     165     161
 
AccI GT'mk_AC

Cuts at:      0    2759    3146    3364    3409    4989    7404 
   Size:       2759     387     218      45    1580    2415

  Fragments arranged by size: 

                2759    2415    1580     387     218      45
 
Acc65I G'GTAC_C

Cuts at:      0    1327    7295    7404 
   Size:       1327    5968     109

  Fragments arranged by size: 

                5968    1327     109
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     296     593     784    1385    1552    1654    1784    1958 
   Size:        296     297     191     601     167     102     130     174

Cuts at:   1958    2261    2555    2620    2717    2792    3068    3098    3143 
   Size:        303     294      65      97      75     276      30      45

Cuts at:   3143    3581    3590    3707    4109    4586    4968    5075    5819 
   Size:        438       9     117     402     477     382     107     744

Cuts at:   5819    5870    6080    6167    6368    6518    6560    7404 
   Size:         51     210      87     201     150      42     844

  Fragments arranged by size: 

                 844     744     601     477     438     402     382     303
                 297     296     294     276     210     201     191     174
                 167     150     130     117     107     102      97      87
                  75      65      51      45      42      30       9
 
AflIII A'CryG_T

Cuts at:      0    2880    4622    7404 
   Size:       2880    1742    2782

  Fragments arranged by size: 

                2880    2782    1742
 
AhdI GACnn_n'nnGTC

Cuts at:      0    4161    5667    7404 
   Size:       4161    1506    1737

  Fragments arranged by size: 

                4161    1737    1506
 
AleI CACnn'nnGTG

Cuts at:      0     308     367    1582    1708    2885    7404 
   Size:        308      59    1215     126    1177    4519

  Fragments arranged by size: 

                4519    1215    1177     308     126      59
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     385    1315    1684    4988    7261    7404 
   Size:        385     930     369    3304    2273     143

  Fragments arranged by size: 

                3304    2273     930     385     369     143
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1542    1807    2380    2419    2815    2842    3163    4336 
   Size:       1542     265     573      39     396      27     321    1173

Cuts at:   4336    4777    6690    7404 
   Size:        441    1913     714

  Fragments arranged by size: 

                1913    1542    1173     714     573     441     396     321
                 265      39      27
 
ApoI r'AATT_y

Cuts at:      0    1348    2773    3334    3472    3649    4090    4942    7123 
   Size:       1348    1425     561     138     177     441     852    2181

Cuts at:   7123    7404 
   Size:        281

  Fragments arranged by size: 

                2181    1425    1348     852     561     441     281     177
                 138
 
AseI AT'TA_AT

Cuts at:      0    6305    7404 
   Size:       6305    1099
 
AvaI C'yCGr_G

Cuts at:      0     991    3416    7404 
   Size:        991    2425    3988

  Fragments arranged by size: 

                3988    2425     991
 
BamHI G'GATC_C

Cuts at:      0    4213    7404 
   Size:       4213    3191
 
BanI G'GyrC_C

Cuts at:      0    1327    1572    3169    4270    7295    7404 
   Size:       1327     245    1597    1101    3025     109

  Fragments arranged by size: 

                3025    1597    1327    1101     245     109
 
BanII G_rGCy'C

Cuts at:      0     182     449     992    1015    2741    3222    3389    3769 
   Size:        182     267     543      23    1726     481     167     380

Cuts at:   3769    4148    4681    5159    5486    6387    7190    7404 
   Size:        379     533     478     327     901     803     214

  Fragments arranged by size: 

                1726     901     803     543     533     481     478     380
                 379     327     267     214     182     167      23
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1912    1946    2591    3011    3131    3326    3650    4415 
   Size:       1912      34     645     420     120     195     324     765

Cuts at:   4415    4550    4625    5593    5686    5839    6548    6730    7136 
   Size:        135      75     968      93     153     709     182     406

Cuts at:   7136    7404 
   Size:        268

  Fragments arranged by size: 

                1912     968     765     709     645     420     406     324
                 268     195     182     153     135     120      93      75
                  34
 
BbvCI CC'TCA_GC

Cuts at:      0    2567    2852    4727    7181    7404 
   Size:       2567     285    1875    2454     223

  Fragments arranged by size: 

                2567    2454    1875     285     223
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    3136    3170    6971    7005    7404 
   Size:       3136      34    3801      34     399

  Fragments arranged by size: 

                3801    3136     399      34      34
 
BclI T'GATC_A

Cuts at:      0     335     380    7404 
   Size:        335      45    7024

  Fragments arranged by size: 

                7024     335      45
 
BfrBI ATG'CAT

Cuts at:      0    2950    7404 
   Size:       2950    4454
 
BglI GCCn_nnn'nGGC

Cuts at:      0      58    1858    2377    4669    7404 
   Size:         58    1800     519    2292    2735

  Fragments arranged by size: 

                2735    2292    1800     519      58
 
BglII A'GATC_T

Cuts at:      0    1377    3658    4915    6711    7323    7404 
   Size:       1377    2281    1257    1796     612      81

  Fragments arranged by size: 

                2281    1796    1377    1257     612      81
 
Bme1580I G_kGCm'C

Cuts at:      0    1577    6508    7052    7404 
   Size:       1577    4931     544     352

  Fragments arranged by size: 

                4931    1577     544     352
 
BmgBI CAC'GTC

Cuts at:      0    3423    7404 
   Size:       3423    3981
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1476    3216    7404 
   Size:       1476    1740    4188

  Fragments arranged by size: 

                4188    1740    1476
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     714    3800    4752    5178    5347    5349    6525    7404 
   Size:        714    3086     952     426     169       2    1176     879

  Fragments arranged by size: 

                3086    1176     952     879     714     426     169       2
            
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     439    1138    1826    2423    2593    2755    2837    3293 
   Size:        439     699     688     597     170     162      82     456

Cuts at:   3293    4298    4982    5073    5587    6380    6587    7404 
   Size:       1005     684      91     514     793     207     817

  Fragments arranged by size: 

                1005     817     793     699     688     684     597     514
                 456     439     207     170     162      91      82
 
Bpu10I CC'TnA_GC

Cuts at:      0    2567    2852    4727    4961    7181    7404 
   Size:       2567     285    1875     234    2220     223

  Fragments arranged by size: 

                2567    2220    1875     285     234     223
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1279    6340    7404 
   Size:       1279    5061    1064

  Fragments arranged by size: 

                5061    1279    1064
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1783    3430    5764    6949    7297    7404 
   Size:       1783    1647    2334    1185     348     107

  Fragments arranged by size: 

                2334    1783    1647    1185     348     107
 
BsaAI yAC'GTr

Cuts at:      0    1719    2883    3670    4625    6581    6934    7155    7404 
   Size:       1719    1164     787     955    1956     353     221     249

  Fragments arranged by size: 

                1956    1719    1164     955     787     353     249     221
            
 
BsaBI GATnn'nnATC

Cuts at:      0    5144    7404 
   Size:       5144    2260
 
BsaHI Gr'CG_yC

Cuts at:      0      64    3844    7404 
   Size:         64    3780    3560

  Fragments arranged by size: 

                3780    3560      64
 
BsaWI w'CCGG_w

Cuts at:      0     439    7404 
   Size:        439    6965
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     599     629    1033    1063    5110    5140    5727    5757 
   Size:        599      30     404      30    4047      30     587      30

Cuts at:   5757    6897    6927    7404 
   Size:       1140      30     477

  Fragments arranged by size: 

                4047    1140     599     587     477     404      30      30
                  30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     160    1966    2222    2743    2860    2869    3067    3079 
   Size:        160    1806     256     521     117       9     198      12

Cuts at:   3079    3106    3190    3322    5134    5219    5252    7198    7404 
   Size:         27      84     132    1812      85      33    1946     206

  Fragments arranged by size: 

                1946    1812    1806     521     256     206     198     160
                 132     117      85      84      33      27      12       9
            
 
BseYI C'CCAG_C

Cuts at:      0    1822    6010    7189    7404 
   Size:       1822    4188    1179     215

  Fragments arranged by size: 

                4188    1822    1179     215
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    5039    6914    7404 
   Size:       5039    1875     490

  Fragments arranged by size: 

                5039    1875     490
 
BsiEI CG_ry'CG

Cuts at:      0       8    6521    7404 
   Size:          8    6513     883

  Fragments arranged by size: 

                6513     883       8
 
BsiHKAI G_wGCw'C

Cuts at:      0     152     182     449     992    4681    5159    7404 
   Size:        152      30     267     543    3689     478    2245

  Fragments arranged by size: 

                3689    2245     543     478     267     152      30
 
BsmI GAATG_Cn'

Cuts at:      0     220    4840    6969    7404 
   Size:        220    4620    2129     435

  Fragments arranged by size: 

                4620    2129     435     220
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    3413    3445    7404 
   Size:       3413      32    3959

  Fragments arranged by size: 

                3959    3413      32
 
Bsp1286I G_dGCh'C

Cuts at:      0     152     182     449     992    1015    1577    2741    3222 
   Size:        152      30     267     543      23     562    1164     481

Cuts at:   3222    3389    3769    4148    4681    5159    5486    6387    6508 
   Size:        167     380     379     533     478     327     901     121

Cuts at:   6508    7052    7190    7404 
   Size:        544     138     214

  Fragments arranged by size: 

                1164     901     562     544     543     533     481     478
                 380     379     327     267     214     167     152     138
                 121      30      23
 
BspHI T'CATG_A

Cuts at:      0    3255    7269    7404 
   Size:       3255    4014     135

  Fragments arranged by size: 

                4014    3255     135
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     161    3249    3537    7239    7404 
   Size:        161    3088     288    3702     165

  Fragments arranged by size: 

                3702    3088     288     165     161
 
BsrBI CCG'CTC

Cuts at:      0    3546    7404 
   Size:       3546    3858
 
BsrDI GCAATG_nn'

Cuts at:      0    5970    7404 
   Size:       5970    1434
 
BsrFI r'CCGG_y

Cuts at:      0    2791    7404 
   Size:       2791    4613
 
BssSI C'ACGA_G

Cuts at:      0     409    5366    6250    7404 
   Size:        409    4957     884    1154

  Fragments arranged by size: 

                4957    1154     884     409
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1438    7404 
   Size:       1438    5966
 
BstEII G'GTnAC_C

Cuts at:      0    3900    7404 
   Size:       3900    3504
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1584    3229    3396    6001    7404 
   Size:       1584    1645     167    2605    1403

  Fragments arranged by size: 

                2605    1645    1584    1403     167
 
BstYI r'GATC_y

Cuts at:      0     293    1377    1557    2502    3658    4213    4531    4915 
   Size:        293    1084     180     945    1156     555     318     384

Cuts at:   4915    6711    7323    7404 
   Size:       1796     612      81

  Fragments arranged by size: 

                1796    1156    1084     945     612     555     384     318
                 293     180      81
 
Bsu36I CC'TnA_GG

Cuts at:      0    1697    2498    2726    3329    3896    3938    5624    6155 
   Size:       1697     801     228     603     567      42    1686     531

Cuts at:   6155    6917    7404 
   Size:        762     487

  Fragments arranged by size: 

                1697    1686     801     762     603     567     531     487
                 228      42
 
BtgI C'CryG_G

Cuts at:      0       8    3395    3635    4136    6653    7404 
   Size:          8    3387     240     501    2517     751

  Fragments arranged by size: 

                3387    2517     751     501     240       8
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0      33    7404 
   Size:         33    7371
 
BtsI GCAGTG_nn'

Cuts at:      0     430     833    1324    3338    3993    6197    7004    7404 
   Size:        430     403     491    2014     655    2204     807     400

  Fragments arranged by size: 

                2204    2014     807     655     491     430     403     400
            
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    5039    5074    7404 
   Size:       5039      35    2330

  Fragments arranged by size: 

                5039    2330      35
 
DraI TTT'AAA

Cuts at:      0    2264    4539    7404 
   Size:       2264    2275    2865

  Fragments arranged by size: 

                2865    2275    2264
 
DraIII CAC_nnn'GTG

Cuts at:      0    5596    7404 
   Size:       5596    1808
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    5934    6574    7035    7404 
   Size:       5934     640     461     369

  Fragments arranged by size: 

                5934     640     461     369
 
EaeI y'GGCC_r

Cuts at:      0    1544    1566    2978    3231    4416    4947    5991    6518 
   Size:       1544      22    1412     253    1185     531    1044     527

Cuts at:   6518    6674    6992    7404 
   Size:        156     318     412

  Fragments arranged by size: 

                1544    1412    1185    1044     531     527     412     318
                 253     156      22
 
EagI C'GGCC_G

Cuts at:      0    6518    7404 
   Size:       6518     886
 
EarI CTCTTCn'nnn_

Cuts at:      0    1117    1826    2027    2066    2141    2282    2516    2531 
   Size:       1117     709     201      39      75     141     234      15

Cuts at:   2531    2585    2822    2858    2959    3044    3443    3636    3757 
   Size:         54     237      36     101      85     399     193     121

Cuts at:   3757    5202    5457    5846    6494    6935    7404 
   Size:       1445     255     389     648     441     469

  Fragments arranged by size: 

                1445    1117     709     648     469     441     399     389
                 255     237     234     201     193     141     121     101
                  85      75      54      39      36      15
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     872    2953    7404 
   Size:        872    2081    4451

  Fragments arranged by size: 

                4451    2081     872
 
EcoICRI GAG'CTC

Cuts at:      0     180     447     990    4679    5157    7404 
   Size:        180     267     543    3689     478    2247

  Fragments arranged by size: 

                3689    2247     543     478     267     180
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     296     439     593     784    1138    1385    1552    1654 
   Size:        296     143     154     191     354     247     167     102

Cuts at:   1654    1784    1826    1958    2261    2423    2555    2593    2620 
   Size:        130      42     132     303     162     132      38      27

Cuts at:   2620    2717    2755    2792    2837    3068    3098    3143    3293 
   Size:         97      38      37      45     231      30      45     150

Cuts at:   3293    3581    3590    3707    4109    4298    4586    4968    4982 
   Size:        288       9     117     402     189     288     382      14

Cuts at:   4982    5073    5075    5587    5819    5870    6080    6167    6368 
   Size:         91       2     512     232      51     210      87     201

Cuts at:   6368    6380    6518    6560    6587    7404 
   Size:         12     138      42      27     817

  Fragments arranged by size: 

                 817     512     402     382     354     303     296     288
                 288     247     232     231     210     201     191     189
                 167     162     154     150     143     138     132     132
                 130     117     102      97      91      87      51      45
                  45      42      42      38      38      37      30      27
                  27      14      12       9       2
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     575    1058    5569    6398    7404 
   Size:        575     483    4511     829    1006

  Fragments arranged by size: 

                4511    1006     829     575     483
 
EcoO109I rG'GnC_Cy

Cuts at:      0    2480    2659    5639    6006    7404 
   Size:       2480     179    2980     367    1398

  Fragments arranged by size: 

                2980    2480    1398     367     179
 
EcoRI G'AATT_C

Cuts at:      0    1348    3334    3472    4090    7404 
   Size:       1348    1986     138     618    3314

  Fragments arranged by size: 

                3314    1986    1348     618     138
 
EcoRV GAT'ATC

Cuts at:      0    3377    5349    7404 
   Size:       3377    1972    2055

  Fragments arranged by size: 

                3377    2055    1972
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     129    7404 
   Size:        129    7275
 
FspI TGC'GCA

Cuts at:      0    1672    7404 
   Size:       1672    5732
 
HaeII r_GCGC'y

Cuts at:      0    2897    7404 
   Size:       2897    4507
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     278     285     310     317     599     631     856     888 
   Size:        278       7      25       7     282      32     225      32

Cuts at:    888    3391    3423    3442    3474    3712    3744    4015    4047 
   Size:       2503      32      19      32     238      32     271      32

Cuts at:   4047    4147    4179    4612    4644    4693    4725    4900    4932 
   Size:        100      32     433      32      49      32     175      32

Cuts at:   4932    5110    5142    5211    5233    5243    5265    5395    5427 
   Size:        178      32      69      22      10      22     130      32

Cuts at:   5427    5458    5490    5653    5685    6775    6807    6835    6867 
   Size:         31      32     163      32    1090      32      28      32

Cuts at:   6867    7251    7283    7404 
   Size:        384      32     121

  Fragments arranged by size: 

                2503    1090     433     384     282     278     271     238
                 225     178     175     163     130     121     100      69
                  49      32      32      32      32      32      32      32
                  32      32      32      32      32      32      32      32
                  32      32      31      28      25      22      22      19
                  10       7       7
 
HincII GTy'rAC

Cuts at:      0    3147    4990    7404 
   Size:       3147    1843    2414

  Fragments arranged by size: 

                3147    2414    1843
 
KpnI G_GTAC'C

Cuts at:      0    1331    7299    7404 
   Size:       1331    5968     105

  Fragments arranged by size: 

                5968    1331     105
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     918    1484    6530    7196    7404 
   Size:        918     566    5046     666     208

  Fragments arranged by size: 

                5046     918     666     566     208
 
MscI TGG'CCA

Cuts at:      0    1546    1568    2980    4418    4949    5993    6676    7404 
   Size:       1546      22    1412    1438     531    1044     683     728

  Fragments arranged by size: 

                1546    1438    1412    1044     728     683     531      22
            
 
MslI CAynn'nnrTG

Cuts at:      0     308     367    1117    1582    1708    2885    3394    4552 
   Size:        308      59     750     465     126    1177     509    1158

Cuts at:   4552    6644    7249    7404 
   Size:       2092     605     155

  Fragments arranged by size: 

                2092    1177    1158     750     605     509     465     308
                 155     126      59
 
MspA1I CmG'CkG

Cuts at:      0     975    2257    2392    2419    2434    2571    3733    6823 
   Size:        975    1282     135      27      15     137    1162    3090

Cuts at:   6823    7404 
   Size:        581

  Fragments arranged by size: 

                3090    1282    1162     975     581     137     135      27
                  15
 
NcoI C'CATG_G

Cuts at:      0    3395    3635    4136    6653    7404 
   Size:       3395     240     501    2517     751

  Fragments arranged by size: 

                3395    2517     751     501     240
 
NdeI CA'TA_TG

Cuts at:      0    4634    5868    7404 
   Size:       4634    1234    1536

  Fragments arranged by size: 

                4634    1536    1234
 
NsiI A_TGCA'T

Cuts at:      0    2952    7404 
   Size:       2952    4452
 
NspI r_CATG'y

Cuts at:      0     969    4726    5082    7248    7391    7404 
   Size:        969    3757     356    2166     143      13

  Fragments arranged by size: 

                3757    2166     969     356     143      13
 
PasI CC'CwG_GG

Cuts at:      0    1008    3813    7404 
   Size:       1008    2805    3591

  Fragments arranged by size: 

                3591    2805    1008
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1242    7404 
   Size:       1242    6162
 
PfoI T'CCnGG_A

Cuts at:      0     795    1493    4802    4891    6396    7202    7404 
   Size:        795     698    3309      89    1505     806     202

  Fragments arranged by size: 

                3309    1505     806     795     698     202      89
 
PmlI CAC'GTG

Cuts at:      0    1719    6934    7404 
   Size:       1719    5215     470

  Fragments arranged by size: 

                5215    1719     470
 
PpuMI rG'GwC_Cy

Cuts at:      0    2480    2659    5639    6006    7404 
   Size:       2480     179    2980     367    1398

  Fragments arranged by size: 

                2980    2480    1398     367     179
 
PshAI GACnn'nnGTC

Cuts at:      0    4848    4985    5066    7404 
   Size:       4848     137      81    2338

  Fragments arranged by size: 

                4848    2338     137      81
 
PspXI vC'TCGA_Gb

Cuts at:      0     991    7404 
   Size:        991    6413
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0    6090    6122    7404 
   Size:       6090      32    1282

  Fragments arranged by size: 

                6090    1282      32
 
PstI C_TGCA'G

Cuts at:      0    2391    3159    3870    5418    6135    6204    7011    7404 
   Size:       2391     768     711    1548     717      69     807     393

  Fragments arranged by size: 

                2391    1548     807     768     717     711     393      69
            
 
PvuII CAG'CTG

Cuts at:      0    2257    2392    2419    2434    2571    3733    7404 
   Size:       2257     135      27      15     137    1162    3671

  Fragments arranged by size: 

                3671    2257    1162     137     135      27      15
 
SacI G_AGCT'C

Cuts at:      0     182     449     992    4681    5159    7404 
   Size:        182     267     543    3689     478    2245

  Fragments arranged by size: 

                3689    2245     543     478     267     182
 
SalI G'TCGA_C

Cuts at:      0    3145    4988    7404 
   Size:       3145    1843    2416

  Fragments arranged by size: 

                3145    2416    1843
 
SanDI GG'GwC_CC

Cuts at:      0    6006    7404 
   Size:       6006    1398
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1826    3757    7404 
   Size:       1826    1931    3647

  Fragments arranged by size: 

                3647    1931    1826
 
SexAI A'CCwGG_T

Cuts at:      0    5592    5635    7404 
   Size:       5592      43    1769

  Fragments arranged by size: 

                5592    1769      43
 
SfcI C'TryA_G

Cuts at:      0    2387    3155    3866    4046    5009    5064    5414    6131 
   Size:       2387     768     711     180     963      55     350     717

Cuts at:   6131    6200    7007    7404 
   Size:         69     807     397

  Fragments arranged by size: 

                2387     963     807     768     717     711     397     350
                 180      69      55
 
SmlI C'TyrA_G

Cuts at:      0     991    1258    3416    6319    7404 
   Size:        991     267    2158    2903    1085

  Fragments arranged by size: 

                2903    2158    1085     991     267
 
SpeI A'CTAG_T

Cuts at:      0    4351    7404 
   Size:       4351    3053
 
SphI G_CATG'C

Cuts at:      0    4726    7391    7404 
   Size:       4726    2665      13

  Fragments arranged by size: 

                4726    2665      13
 
SspI AAT'ATT

Cuts at:      0     633    7404 
   Size:        633    6771
 
StuI AGG'CCT

Cuts at:      0    5182    6670    7058    7404 
   Size:       5182    1488     388     346

  Fragments arranged by size: 

                5182    1488     388     346
 
StyI C'CwwG_G

Cuts at:      0    1898    2510    2933    3395    3635    4136    6653    6671 
   Size:       1898     612     423     462     240     501    2517      18

Cuts at:   6671    6800    7404 
   Size:        129     604

  Fragments arranged by size: 

                2517    1898     612     604     501     462     423     240
                 129      18
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     157     547     920     943    1495    1564    1746    3008 
   Size:        157     390     373      23     552      69     182    1262

Cuts at:   3008    3805    4860    5005    5149    5692    6487    7404 
   Size:        797    1055     145     144     543     795     917

  Fragments arranged by size: 

                1262    1055     917     797     795     552     543     390
                 373     182     157     145     144      69      23
 
TatI w'GTAC_w

Cuts at:      0    1484    7404 
   Size:       1484    5920
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     519     551    2643    2675    5727    5759    6897    6929 
   Size:        519      32    2092      32    3052      32    1138      32

Cuts at:   6929    7404 
   Size:        475

  Fragments arranged by size: 

                3052    2092    1138     519     475      32      32      32
                  32
 
Tth111I GACn'n_nGTC

Cuts at:      0    3456    6756    7035    7404 
   Size:       3456    3300     279     369

  Fragments arranged by size: 

                3456    3300     369     279
 
XbaI T'CTAG_A

Cuts at:      0    1521    5938    7404 
   Size:       1521    4417    1466

  Fragments arranged by size: 

                4417    1521    1466
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    3705    4665    5562    7404 
   Size:       3705     960     897    1842

  Fragments arranged by size: 

                3705    1842     960     897
 
XhoI C'TCGA_G

Cuts at:      0     991    3416    7404 
   Size:        991    2425    3988

  Fragments arranged by size: 

                3988    2425     991
 
XmnI GAAnn'nnTTC

Cuts at:      0     835    1352    4235    7404 
   Size:        835     517    2883    3169

  Fragments arranged by size: 

                3169    2883     835     517
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AcuI   AflIII     AhdI     AleI     AlfI
    AlwNI     ApoI     AseI     AvaI    BamHI     BanI    BanII     BbsI
    BbvCI     BcgI     BclI    BfrBI     BglI    BglII Bme1580I    BmgBI
     BmrI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI
    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI
     BsmI    BsmBI Bsp1286I    BspHI    BspMI    BsrBI    BsrDI    BsrFI
    BssSI   BstAPI   BstEII    BstXI    BstYI   Bsu36I     BtgI    BtgZI
     BtsI    CspCI     DraI   DraIII     DrdI     EaeI     EagI     EarI
     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI    EcoRV     FalI
     FspI    HaeII    Hin4I   HincII     KpnI     MmeI     MscI     MslI
   MspA1I     NcoI     NdeI     NsiI     NspI     PasI    PflMI     PfoI
     PmlI    PpuMI    PshAI    PspXI     PsrI     PstI    PvuII     SacI
     SalI    SanDI     SapI    SexAI     SfcI     SmlI     SpeI     SphI
     SspI     StuI     StyI    TaqII     TatI     TstI  Tth111I     XbaI
     XcmI     XhoI     XmnI

 Enzymes that do not cut: 

    AatII     AclI     AfeI    AflII     AgeI     AloI     ApaI    ApaLI
     AscI    AsiSI    AvrII     BaeI     BbeI    BciVI     BlpI     BmtI
    BsiWI    BspEI    BsrGI   BssHII    BstBI  BstZ17I     ClaI     FseI
    FspAI  HindIII     HpaI     KasI     MfeI     MluI     NaeI     NarI
   NgoMIV     NheI     NotI     NruI     PacI     PciI     PmeI     PpiI
     PsiI   PspOMI     PvuI    RsrII    SacII     SbfI     ScaI     SfiI
     SfoI     SgfI    SgrAI     SmaI    SnaBI     SrfI     SwaI     XmaI
     ZraI
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