Restriction map for pF1KB5680
##################################
# NO CUT ENZYMES (  116 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AflII       AgeI        Alw44I      Aor51HI     
ApaLI       ApoI        AscI        AseI        AsnI        
Asp700I     Asp718I     AspI        AviII       AvrII       
BamHI       BbeI        BbvCI       BcgI        BciVI       
BfaI        BfrI        BglI        BglII       BlnI        
BsaI        BseAI       BsiWI       BsmBI       BsmI        
Bsp1407I    BspDI       BspEI       BspHI       BspLU11I    
BsrDI       BsrGI       BssHII      BssSI       Bst1107I    
BstAPI      BstBI       BstEII      BstPI       BstXI       
BstZ17I     ClaI        CpoI        DraI        DrdI        
DsaI        Eco47III    EcoO65I     EcoRI       EcoRV       
EcoT22I     FseI        FspI        HincII      HindII      
HpaI        KasI        KpnI        KspI        MaeI        
MfeI        MluI        MroI        MunI        NaeI        
NarI        NcoI        NdeI        NgoMIV      NheI        
NruI        NsiI        NspI        NspV        PacI        
PaeR7I      PflFI       PinAI       PmeI        PshBI       
Psp1406I    PvuI        RcaI        RsrII       SacII       
SalI        SapI        SbfI        SfiI        SfoI        
SfuI        SgrAI       SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        StuI        SwaI        
TfiI        Tsp509I     Tth111I     XbaI        XhoI        
XmnI        


###################################
# ONE CUT ENZYMES (   52 enzymes) #
###################################

AcyI         1192  GACGCC               G[GA]^CG[CT]C
AflIII       1244  ACGTGT               A^C[GA][CT]GT
AhdI          355  GACTTCCGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI         386  CAGGGGCTG            CAG[ACGT][ACGT][ACGT]^CTG
AocI          277  CCTGAGG              CC^T[ACGT]AGG
ApaI          746  GGGCCC               GGGCC^C
AspEI         355  GACTTCCGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI        1075  GAGCTC               G[AT]GC[AT]^C
BbrPI        1243  CACGTG               CAC^GTG
BclI         1337  TGATCA               T^GATCA
BlpI         1260  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I     1260  GCTCAGC              GC^T[ACGT]AGC
BsaAI        1243  CACGTG               [CT]AC^GT[GA]
BsaBI        1338  GATCACCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI        1192  GACGCC               G[GA]^CG[CT]C
BsaWI         359  TCCGGT               [AT]^CCGG[AT]
BsiHKAI      1075  GAGCTC               G[AT]GC[AT]^C
BspMI         788  ACCTGC               ACCTGC(4/8)
BsrFI        1225  GCCGGT               [GA]^CCGG[CT]
Bsu36I        277  CCTGAGG              CC^T[ACGT]AGG
CelII        1260  GCTCAGC              GC^T[ACGT]AGC
Cfr10I       1225  GCCGGT               [GA]^CCGG[CT]
Eam1105I      355  GACTTCCGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI          394  GAAGAG               CTCTTC(1/4)
Eco81I        277  CCTGAGG              CC^T[ACGT]AGG
FbaI         1337  TGATCA               T^GATCA
HaeII         760  GGCGCT               [GA]GCGC^[CT]
Hin1I        1192  GACGCC               G[GA]^CG[CT]C
HindIII      1066  AAGCTT               A^AGCTT
HinfI         273  GACTC                G^A[ACGT]TC
Ksp632I       394  GAAGAG               CTCTTC(1/4)
MamI         1338  GATCACCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MseI          863  TTAA                 T^TAA
MslI         1080  CATCCTCATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NotI          120  GCGGCCGC             GC^GGCCGC
PflMI         309  CCAGACTGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI          273  GACTC                GAGTC(4/5)
PmaCI        1243  CACGTG               CAC^GTG
PmlI         1243  CACGTG               CAC^GTG
PshAI         240  GACCACTGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII         181  CAGCTG               CAG^CTG
SacI         1075  GAGCTC               GAGCT^C
ScaI          641  AGTACT               AGT^ACT
SexAI        1303  ACCAGGT              A^CC[AT]GGT
SfaNI        1212  GCATC                GCATC(5/9)
SmaI          719  CCCGGG               CCC^GGG
TaqI          251  TCGA                 T^CGA
Tru9I         863  TTAA                 T^TAA
TthHB8I       251  TCGA                 T^CGA
Van91I        309  CCAGACTGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI         719  CCCGGG               C^CCGGG
XmaI          719  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   20 enzymes) #
###################################

BalI          413  TGGCCA               TGG^CCA
              560  TGGCCA              
BbsI          189  GAAGAC               GAAGAC(2/6)
              918  GAAGAC              
BpuAI         189  GAAGAC               GAAGAC(2/6)
              918  GAAGAC              
BsmAI         271  GAGAC                GTCTC(1/5)
             1294  GAGAC               
BstYI         536  AGATCC               [GA]^GATC[CT]
             1094  AGATCC              
DraIII        371  CACCCAGTG            CAC[ACGT][ACGT][ACGT]^GTG
              427  CACCGCGTG           
EagI          121  CGGCCG               C^GGCCG
              978  CGGCCG              
EclXI         121  CGGCCG               C^GGCCG
              978  CGGCCG              
Eco52I        121  CGGCCG               C^GGCCG
              978  CGGCCG              
EcoT14I       563  CCAAGG               C^C[AT][AT]GG
             1196  CCAAGG              
HgaI         1192  GACGC                GACGC(5/10)
              599  GCGTC               
MflI          536  AGATCC               [GA]^GATC[CT]
             1094  AGATCC              
MluNI         413  TGGCCA               TGG^CCA
              560  TGGCCA              
MscI          413  TGGCCA               TGG^CCA
              560  TGGCCA              
PpuMI         818  GGGACCT              [GA]G^G[AT]CC[CT]
             1157  AGGACCC             
PstI          232  CTGCAG               CTGCA^G
              604  CTGCAG              
SfcI          232  CTGCAG               C^T[GA][CT]AG
              604  CTGCAG              
StyI          563  CCAAGG               C^C[AT][AT]GG
             1196  CCAAGG              
XcmI          305  CCATCCAGACTGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
              563  CCAAGGACGAGGTGG     
XhoII         536  AGATCC               [GA]^GATC[CT]
             1094  AGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   70 enzymes) #
#########################################

AccII       AciI        AfaI        AluI        AlwI        
AvaI        AvaII       BanI        BanII       BbvI        
BcnI        BmyI        BpmI        BsaJI       BseRI       
BsgI        BsiEI       BsiYI       BslI        BsmFI       
BsoBI       Bsp1286I    BsrBI       BsrI        BssKI       
BstF5I      BstNI       BstUI       Cac8I       CfoI        
Cfr13I      DdeI        DpnII       DraII       EaeI        
EcoNI       EcoO109I    EcoRII      FauI        Fnu4HI      
FokI        HaeIII      HapII       HhaI        HinP1I      
HpaII       HphI        ItaI        MaeII       MaeIII      
MboI        MboII       MnlI        MspA1I      MspI        
MvaI        MvnI        MwoI        NciI        NdeII       
NlaIII      NlaIV       RsaI        Sau3AI      Sau96I      
ScrFI       SmlI        TseI        Tsp45I      TspRI       
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