Restriction map for pF1KB5656
##################################
# NO CUT ENZYMES (  132 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AfaI        AflII       AflIII      AgeI        
Alw44I      ApaI        ApaLI       AscI        AseI        
AsnI        Asp718I     AspI        AvrII       BalI        
BamHI       BbvCI       BcgI        BciVI       BfrI        
BglII       BlnI        BlpI        Bpu1102I    BsaBI       
BsaWI       BseAI       BsiWI       BsmBI       Bsp1407I    
BspDI       BspEI       BspLU11I    BsrFI       BsrGI       
BssHII      BssSI       Bst1107I    BstAPI      BstBI       
BstEII      BstPI       BstUI       BstXI       BstZ17I     
CelII       Cfr10I      ClaI        CpoI        DraI        
DraII       DraIII      DrdI        DsaI        EaeI        
EagI        EclXI       Eco52I      EcoO109I    EcoO65I     
EcoRV       EcoT22I     FauI        FseI        HincII      
HindII      HindIII     HpaI        KpnI        KspI        
MamI        MfeI        MluI        MluNI       MroI        
MscI        MspA1I      MunI        MvnI        NaeI        
NcoI        NdeI        NgoMIV      NheI        NotI        
NruI        NsiI        NspV        PacI        PaeR7I      
PflFI       PflMI       PinAI       PmeI        PpuMI       
PshAI       PshBI       Psp1406I    PstI        PvuII       
RsaI        RsrII       SacII       SalI        SapI        
SbfI        ScaI        SexAI       SfiI        SfuI        
SgrAI       SmaI        SnaBI       SpeI        Sse8387I    
SspBI       SspI        SwaI        TfiI        Tsp45I      
Tth111I     Van91I      XbaI        XcmI        XhoI        
XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   68 enzymes) #
###################################

AcyI          121  GGCGCC               G[GA]^CG[CT]C
AhdI          374  GACCAGAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI          436  CCTGAGG              CC^T[ACGT]AGG
Aor51HI       449  AGCGCT               AGC^GCT
Asp700I       355  GAACAACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI         374  GACCAGAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI         703  GAGCTC               G[AT]GC[AT]^C
AvaI          692  CTCGGG               C^[CT]CG[GA]G
AviII         779  TGCGCA               TGC^GCA
BanI          121  GGCGCC               G^G[CT][GA]CC
BanII         703  GAGCTC               G[GA]GC[CT]^C
BbeI          121  GGCGCC               GGCGC^C
BbrPI         766  CACGTG               CAC^GTG
BclI          606  TGATCA               T^GATCA
BcnI          590  CCCGG                CC^[GC]GG
BfaI           17  CTAG                 C^TAG
BglI          589  GCCCGGCAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BmyI          703  GAGCTC               G[AGT]GC[ACT]^C
BsaAI         766  CACGTG               [CT]AC^GT[GA]
BsaHI         121  GGCGCC               G[GA]^CG[CT]C
BsgI          958  CTGCAC               GTGCAG(16/14)
BsiEI        1035  CGATCG               CG[GA][CT]^CG
BsiHKAI       703  GAGCTC               G[AT]GC[AT]^C
BsmFI         382  GTCCC                GGGAC(10/14)
BsmI          747  GAATGC               GAATGC(1/-1)
BsoBI         692  CTCGGG               C^[CT]CG[GA]G
Bsp1286I      703  GAGCTC               G[AGT]GC[ACT]^C
BspMI         867  GCAGGT               ACCTGC(4/8)
BsrBI         820  GAGCGG               CCGCTC(-3/-3)
BstYI         159  GGATCT               [GA]^GATC[CT]
Bsu36I        436  CCTGAGG              CC^T[ACGT]AGG
DdeI          437  CTGAG                C^T[ACGT]AG
Eam1105I      374  GACCAGAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI          287  CTCTTC               CTCTTC(1/4)
Eco47III      449  AGCGCT               AGC^GCT
Eco81I        436  CCTGAGG              CC^T[ACGT]AGG
EcoNI         861  CCTGCGGCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI         670  GAATTC               G^AATTC
EcoT14I        59  CCAAGG               C^C[AT][AT]GG
FbaI          606  TGATCA               T^GATCA
FspI          779  TGCGCA               TGC^GCA
HapII         591  CCGG                 C^CGG
HgaI          348  GCGTC                GACGC(5/10)
Hin1I         121  GGCGCC               G[GA]^CG[CT]C
HpaII         591  CCGG                 C^CGG
HphI           96  TCACC                GGTGA(8/7)
KasI          121  GGCGCC               G^GCGCC
Ksp632I       287  CTCTTC               CTCTTC(1/4)
MaeI           17  CTAG                 C^TAG
MaeII         767  ACGT                 A^CGT
MaeIII        485  GTTAC                ^GT[ACGT]AC
MflI          159  GGATCT               [GA]^GATC[CT]
MseI           91  TTAA                 T^TAA
MspI          591  CCGG                 C^CGG
NarI          121  GGCGCC               GG^CGCC
NciI          590  CCCGG                CC^[GC]GG
PmaCI         766  CACGTG               CAC^GTG
PmlI          766  CACGTG               CAC^GTG
PvuI         1035  CGATCG               CGAT^CG
SacI          703  GAGCTC               GAGCT^C
SfaNI         583  GATGC                GCATC(5/9)
SfoI          121  GGCGCC               GGC^GCC
SmlI          167  CTCAAG               C^T[CT][GA]AG
StuI          521  AGGCCT               AGG^CCT
StyI           59  CCAAGG               C^C[AT][AT]GG
Tru9I          91  TTAA                 T^TAA
XhoII         159  GGATCT               [GA]^GATC[CT]
XmnI          355  GAACAACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   18 enzymes) #
###################################

AcsI          148  GAATTT               [GA]^AATT[CT]
              670  GAATTC              
AlwI          813  GATCC                GGATC(4/5)
              159  GGATC               
AlwNI         847  CAGCACCTG            CAG[ACGT][ACGT][ACGT]^CTG
              868  CAGGTGCTG           
ApoI          148  GAATTT               [GA]^AATT[CT]
              670  GAATTC              
AvaII         260  GGTCC                G^G[AT]CC
              552  GGACC               
BpmI          799  CTGGAG               CTGGAG(16/14)
              853  CTGGAG              
BsaI          372  GAGACC               GGTCTC(1/5)
              856  GAGACC              
BsaJI          59  CCAAGG               C^C[ACGT][ACGT]GG
              548  CCCAGG              
BseRI         251  GAGGAG               GAGGAG(10/8)
              739  GAGGAG              
BsrI           85  ACTGG                ACTGG(1/-1)
              498  ACTGG               
Cfr13I        260  GGTCC                G^G[ACGT]CC
              552  GGACC               
HaeII         121  GGCGCC               [GA]GCGC^[CT]
              449  AGCGCT              
HaeIII        522  GGCC                 GG^CC
              597  GGCC                
NspI          776  GCATGC               [GA]CATG^[CT]
              893  GCATGC              
Sau96I        260  GGTCC                G^G[ACGT]CC
              552  GGACC               
SfcI          923  CTACAG               C^T[GA][CT]AG
             1007  CTACAG              
SphI          776  GCATGC               GCATG^C
              893  GCATGC              
Tsp509I       149  AATT                 ^AATT
              671  AATT                


#########################################
# 3 OR MORE CUT ENZYMES (   40 enzymes) #
#########################################

AciI        AluI        BbsI        BbvI        BpuAI       
BsiYI       BslI        BsmAI       BspHI       BsrDI       
BssKI       BstF5I      BstNI       Cac8I       CfoI        
DpnII       EcoRII      Fnu4HI      FokI        HhaI        
HinP1I      HinfI       ItaI        MboI        MboII       
MnlI        MslI        MvaI        MwoI        NdeII       
NlaIII      NlaIV       PleI        RcaI        Sau3AI      
ScrFI       TaqI        TseI        TspRI       TthHB8I     
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