Restriction map for pF1KB5636
##################################
# NO CUT ENZYMES (  130 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AfaI        AflII       AflIII      AgeI        
AhdI        Alw44I      AocI        Aor51HI     ApaLI       
ApoI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AspI        AviII       AvrII       BamHI       
BbrPI       BbsI        BcgI        BciVI       BclI        
BfrI        BglII       BlnI        BpuAI       BsaAI       
BsaBI       BsaWI       BseAI       BsiWI       BsmI        
Bsp1407I    BspDI       BspEI       BspHI       BspLU11I    
BspMI       BsrDI       BsrGI       BssSI       Bst1107I    
BstBI       BstEII      BstPI       BstXI       BstZ17I     
Bsu36I      ClaI        CpoI        DraI        DraIII      
DrdI        DsaI        Eam1105I    Eco47III    Eco81I      
EcoNI       EcoO65I     EcoRI       EcoRV       EcoT22I     
FbaI        FseI        FspI        HincII      HindII      
HindIII     HpaI        KpnI        KspI        MamI        
MfeI        MluI        MroI        MslI        MunI        
NcoI        NdeI        NheI        NruI        NsiI        
NspI        NspV        PacI        PaeR7I      PflFI       
PinAI       PmaCI       PmeI        PmlI        PshAI       
PshBI       Psp1406I    PstI        PvuI        RcaI        
RsaI        RsrII       SacII       SalI        SapI        
SbfI        ScaI        SfuI        SgrAI       SmlI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        StuI        SwaI        TfiI        Tsp509I     
Tth111I     XbaI        XcmI        XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   45 enzymes) #
###################################

AlwNI         424  CAGCCGCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApaI          292  GGGCCC               GGGCC^C
AscI          820  GGCGCGCC             GG^CGCGCC
AspHI         625  GAGCTC               G[AT]GC[AT]^C
BbeI          205  GGCGCC               GGCGC^C
BbvCI         417  CCTCAGC              CCTCAGC(-5/-2)
BfaI           46  CTAG                 C^TAG
BlpI          666  GCTCAGC              GC^T[ACGT]AGC
BpmI          864  CTCCAG               CTGGAG(16/14)
Bpu1102I      666  GCTCAGC              GC^T[ACGT]AGC
BsaI          507  GAGACC               GGTCTC(1/5)
BsiHKAI       625  GAGCTC               G[AT]GC[AT]^C
BsmBI         118  GAGACG               CGTCTC(1/5)
BsrBI           7  GAGCGG               CCGCTC(-3/-3)
BstAPI        843  GCAAGACGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI         680  AGATCC               [GA]^GATC[CT]
CelII         666  GCTCAGC              GC^T[ACGT]AGC
EarI          501  GAAGAG               CTCTTC(1/4)
EcoT14I       113  CCAAGG               C^C[AT][AT]GG
HaeII         205  GGCGCC               [GA]GCGC^[CT]
HinfI         363  GAGTC                G^A[ACGT]TC
KasI          205  GGCGCC               G^GCGCC
Ksp632I       501  GAAGAG               CTCTTC(1/4)
MaeI           46  CTAG                 C^TAG
MaeII         848  ACGT                 A^CGT
MflI          680  AGATCC               [GA]^GATC[CT]
MseI          220  TTAA                 T^TAA
NaeI          982  GCCGGC               GCC^GGC
NarI          205  GGCGCC               GG^CGCC
NgoMIV        982  GCCGGC               G^CCGGC
PflMI         423  CCAGCCGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI          363  GAGTC                GAGTC(4/5)
PpuMI         773  AGGACCC              [GA]G^G[AT]CC[CT]
SacI          625  GAGCTC               GAGCT^C
SexAI          89  ACCTGGT              A^CC[AT]GGT
SfaNI         466  GATGC                GCATC(5/9)
SfcI          411  CTACAG               C^T[GA][CT]AG
SfiI           69  GGCCAGCATGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI          205  GGCGCC               GGC^GCC
StyI          113  CCAAGG               C^C[AT][AT]GG
Tru9I         220  TTAA                 T^TAA
Tsp45I         35  GTGAC                ^GT[GC]AC
TspRI          33  CAGTG                CA[GC]TG[ACGT][ACGT]^
Van91I        423  CCAGCCGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII         680  AGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   29 enzymes) #
###################################

AcyI          106  GGCGTC               G[GA]^CG[CT]C
              205  GGCGCC              
AlwI          639  GATCC                GGATC(4/5)
              681  GATCC               
AvaI          177  CCCGGG               C^[CT]CG[GA]G
              289  CCCGGG              
BalI          692  TGGCCA               TGG^CCA
              716  TGGCCA              
BglI           70  GCCAGCATGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
              176  GCCCGGGGGGC         
BsaHI         106  GGCGTC               G[GA]^CG[CT]C
              205  GGCGCC              
BsgI          279  GTGCAG               GTGCAG(16/14)
              355  GTGCAG              
BsoBI         177  CCCGGG               C^[CT]CG[GA]G
              289  CCCGGG              
BsrFI         193  GCCGGT               [GA]^CCGG[CT]
              982  GCCGGC              
Cfr10I        193  GCCGGT               [GA]^CCGG[CT]
              982  GCCGGC              
DpnII         639  GATC                 ^GATC
              681  GATC                
DraII         613  GGGGCCT              [GA]G^G[ACGT]CC[CT]
              773  AGGACCC             
EcoO109I      613  GGGGCCT              [GA]G^G[ACGT]CC[CT]
              773  AGGACCC             
Hin1I         106  GGCGTC               G[GA]^CG[CT]C
              205  GGCGCC              
HphI          482  TCACC                GGTGA(8/7)
              572  TCACC               
MaeIII         35  GTGAC                ^GT[ACGT]AC
              322  GTTAC               
MboI          639  GATC                 ^GATC
              681  GATC                
MboII         501  GAAGA                GAAGA(8/7)
              569  TCTTC               
MluNI         692  TGGCCA               TGG^CCA
              716  TGGCCA              
MscI          692  TGGCCA               TGG^CCA
              716  TGGCCA              
NdeII         639  GATC                 ^GATC
              681  GATC                
NotI            9  GCGGCCGC             GC^GGCCGC
              130  GCGGCCGC            
PvuII         358  CAGCTG               CAG^CTG
              867  CAGCTG              
Sau3AI        639  GATC                 ^GATC
              681  GATC                
SmaI          177  CCCGGG               CCC^GGG
              289  CCCGGG              
TaqI          155  TCGA                 T^CGA
              495  TCGA                
TthHB8I       155  TCGA                 T^CGA
              495  TCGA                
XmaCI         177  CCCGGG               C^CCGGG
              289  CCCGGG              
XmaI          177  CCCGGG               C^CCGGG
              289  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (   54 enzymes) #
#########################################

AccII       AciI        AluI        AvaII       BanI        
BanII       BbvI        BcnI        BmyI        BsaJI       
BseRI       BsiEI       BsiYI       BslI        BsmAI       
BsmFI       Bsp1286I    BsrI        BssHII      BssKI       
BstF5I      BstNI       BstUI       Cac8I       CfoI        
Cfr13I      DdeI        EaeI        EagI        EclXI       
Eco52I      EcoRII      FauI        Fnu4HI      FokI        
HaeIII      HapII       HgaI        HhaI        HinP1I      
HpaII       ItaI        MnlI        MspA1I      MspI        
MvaI        MvnI        MwoI        NciI        NlaIII      
NlaIV       Sau96I      ScrFI       TseI        
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