Restriction map for pF1KB5636
 (Linear) (Six-base) MAPSORT of: KIBB5636.seq  Check: 271  from: 1  to: 993



KIBB5636.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 10:58  ..

AlwNI CAG_nnn'CTG

Cuts at:      0     429     993 
   Size:        429     564
 
ApaI G_GGCC'C

Cuts at:      0     296     993 
   Size:        296     697
 
AscI GG'CGCG_CC

Cuts at:      0     821     993 
   Size:        821     172
 
AvaI C'yCGr_G

Cuts at:      0     177     289     993 
   Size:        177     112     704

  Fragments arranged by size: 

                 704     177     112
 
BanI G'GyrC_C

Cuts at:      0     205     261     748     993 
   Size:        205      56     487     245

  Fragments arranged by size: 

                 487     245     205      56
 
BanII G_rGCy'C

Cuts at:      0     146     296     629     923     993 
   Size:        146     150     333     294      70

  Fragments arranged by size: 

                 333     294     150     146      70
 
BbeI G_GCGC'C

Cuts at:      0     209     993 
   Size:        209     784
 
BbvCI CC'TCA_GC

Cuts at:      0     418     993 
   Size:        418     575
 
BglI GCCn_nnn'nGGC

Cuts at:      0      76     182     993 
   Size:         76     106     811

  Fragments arranged by size: 

                 811     106      76
 
BlpI GC'TnA_GC

Cuts at:      0     667     993 
   Size:        667     326
 
Bme1580I G_kGCm'C

Cuts at:      0     266     296     751     993 
   Size:        266      30     455     242

  Fragments arranged by size: 

                 455     266     242      30
 
BmgBI CAC'GTC

Cuts at:      0     849     993 
   Size:        849     144
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     142     169     993 
   Size:        142      27     824

  Fragments arranged by size: 

                 824     142      27
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     849     993 
   Size:        849     144
 
Bpu10I CC'TnA_GC

Cuts at:      0     418     993 
   Size:        418     575
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     501     993 
   Size:        501     492
 
BsaHI Gr'CG_yC

Cuts at:      0     107     206     993 
   Size:        107      99     787

  Fragments arranged by size: 

                 787     107      99
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     519     754     757     931     993 
   Size:        519     235       3     174      62

  Fragments arranged by size: 

                 519     235     174      62       3
 
BseYI C'CCAG_C

Cuts at:      0      41     752     993 
   Size:         41     711     241

  Fragments arranged by size: 

                 711     241      41
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     300     376     993 
   Size:        300      76     617

  Fragments arranged by size: 

                 617     300      76
 
BsiEI CG_ry'CG

Cuts at:      0      13      65     134     245     348     399     993 
   Size:         13      52      69     111     103      51     594

  Fragments arranged by size: 

                 594     111     103      69      52      51      13
 
BsiHKAI G_wGCw'C

Cuts at:      0     629     993 
   Size:        629     364
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     112     993 
   Size:        112     881
 
Bsp1286I G_dGCh'C

Cuts at:      0     146     266     296     629     751     923     993 
   Size:        146     120      30     333     122     172      70

  Fragments arranged by size: 

                 333     172     146     122     120      70      30
 
BsrBI CCG'CTC

Cuts at:      0       9     993 
   Size:          9     984
 
BsrFI r'CCGG_y

Cuts at:      0     193     982     993 
   Size:        193     789      11

  Fragments arranged by size: 

                 789     193      11
 
BssHII G'CGCG_C

Cuts at:      0     164     166     250     401     821     993 
   Size:        164       2      84     151     420     172

  Fragments arranged by size: 

                 420     172     164     151      84       2
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     849     993 
   Size:        849     144
 
BstYI r'GATC_y

Cuts at:      0     680     993 
   Size:        680     313
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     603     993 
   Size:        603     390
 
BtsI GCAGTG_nn'

Cuts at:      0      39     993 
   Size:         39     954
 
EaeI y'GGCC_r

Cuts at:      0      10      62     131     242     345     396     692     716 
   Size:         10      52      69     111     103      51     296      24

Cuts at:    716     993 
   Size:        277

  Fragments arranged by size: 

                 296     277     111     103      69      52      51      24
                  10
 
EagI C'GGCC_G

Cuts at:      0      10      62     131     242     345     396     993 
   Size:         10      52      69     111     103      51     597

  Fragments arranged by size: 

                 597     111     103      69      52      51      10
 
EarI CTCTTCn'nnn_

Cuts at:      0     496     993 
   Size:        496     497
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     676     993 
   Size:        676     317
 
EcoICRI GAG'CTC

Cuts at:      0     627     993 
   Size:        627     366
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     849     993 
   Size:        849     144
 
EcoO109I rG'GnC_Cy

Cuts at:      0     614     774     993 
   Size:        614     160     219

  Fragments arranged by size: 

                 614     219     160
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     868     993 
   Size:        868     125
 
HaeII r_GCGC'y

Cuts at:      0     209     993 
   Size:        209     784
 
KasI G'GCGC_C

Cuts at:      0     205     993 
   Size:        205     788
 
MscI TGG'CCA

Cuts at:      0     694     718     993 
   Size:        694      24     275

  Fragments arranged by size: 

                 694     275      24
 
MspA1I CmG'CkG

Cuts at:      0     139     328     360     429     446     756     869     993 
   Size:        139     189      32      69      17     310     113     124

  Fragments arranged by size: 

                 310     189     139     124     113      69      32      17
            
 
NaeI GCC'GGC

Cuts at:      0     984     993 
   Size:        984       9
 
NarI GG'CG_CC

Cuts at:      0     206     993 
   Size:        206     787
 
NgoMIV G'CCGG_C

Cuts at:      0     982     993 
   Size:        982      11
 
NotI GC'GGCC_GC

Cuts at:      0      10     131     993 
   Size:         10     121     862

  Fragments arranged by size: 

                 862     121      10
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     429     993 
   Size:        429     564
 
PpuMI rG'GwC_Cy

Cuts at:      0     774     993 
   Size:        774     219
 
PspOMI G'GGCC_C

Cuts at:      0     292     993 
   Size:        292     701
 
PvuII CAG'CTG

Cuts at:      0     360     869     993 
   Size:        360     509     124

  Fragments arranged by size: 

                 509     360     124
 
SacI G_AGCT'C

Cuts at:      0     629     993 
   Size:        629     364
 
SexAI A'CCwGG_T

Cuts at:      0      89     993 
   Size:         89     904
 
SfcI C'TryA_G

Cuts at:      0     411     993 
   Size:        411     582
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0      76     993 
   Size:         76     917
 
SfoI GGC'GCC

Cuts at:      0     207     993 
   Size:        207     786
 
SmaI CCC'GGG

Cuts at:      0     179     291     993 
   Size:        179     112     702

  Fragments arranged by size: 

                 702     179     112
 
StyI C'CwwG_G

Cuts at:      0     113     993 
   Size:        113     880
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      19     766     993 
   Size:         19     747     227

  Fragments arranged by size: 

                 747     227      19
 
XmaI C'CCGG_G

Cuts at:      0     177     289     993 
   Size:        177     112     704

  Fragments arranged by size: 

                 704     177     112
 

 Enzymes that do cut:

    AlwNI     ApaI     AscI     AvaI     BanI    BanII     BbeI    BbvCI
     BglI     BlpI Bme1580I    BmgBI     BmrI     BpmI   Bpu10I     BsaI
    BsaHI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI    BsmBI Bsp1286I
    BsrBI    BsrFI   BssHII   BstAPI    BstYI    BtgZI     BtsI     EaeI
     EagI     EarI     EciI  EcoICRI  Eco57MI EcoO109I     FalI    HaeII
     KasI     MscI   MspA1I     NaeI     NarI   NgoMIV     NotI    PflMI
    PpuMI   PspOMI    PvuII     SacI    SexAI     SfcI     SfiI     SfoI
     SmaI     StyI    TaqII     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI    ApaLI     ApoI
     AseI    AsiSI    AvrII     BaeI    BamHI     BbsI     BcgI    BciVI
     BclI    BfrBI    BglII     BmtI     BplI    BpuEI    BsaAI    BsaBI
    BsaWI    BsaXI    BsiWI     BsmI    BspEI    BspHI    BspMI    BsrDI
    BsrGI    BssSI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I     BtgI
     ClaI    CspCI     DraI   DraIII     DrdI    EcoNI    EcoRI    EcoRV
     FseI     FspI    FspAI    Hin4I   HincII  HindIII     HpaI     KpnI
     MfeI     MluI     MmeI     MslI     NcoI     NdeI     NheI     NruI
     NsiI     NspI     PacI     PasI     PciI     PfoI     PmeI     PmlI
     PpiI    PshAI     PsiI    PspXI     PsrI     PstI     PvuI    RsrII
    SacII     SalI    SanDI     SapI     SbfI     ScaI     SgfI    SgrAI
     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI
     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmnI     ZraI
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