Restriction map for pF1KB5554
 (Linear) (Six-base) MAPSORT of: KIBB5554.seq  Check: 467  from: 1  to: 894



KIBB5554.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 10:58  ..

AccI GT'mk_AC

Cuts at:      0      35     894 
   Size:         35     859
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     217     460     894 
   Size:        217     243     434

  Fragments arranged by size: 

                 434     243     217
 
AflIII A'CryG_T

Cuts at:      0     621     894 
   Size:        621     273
 
AhdI GACnn_n'nnGTC

Cuts at:      0     733     771     894 
   Size:        733      38     123

  Fragments arranged by size: 

                 733     123      38
 
ApaLI G'TGCA_C

Cuts at:      0     224     894 
   Size:        224     670
 
ApoI r'AATT_y

Cuts at:      0     305     894 
   Size:        305     589
 
BanI G'GyrC_C

Cuts at:      0     846     894 
   Size:        846      48
 
BanII G_rGCy'C

Cuts at:      0     158     511     894 
   Size:        158     353     383

  Fragments arranged by size: 

                 383     353     158
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      29     685     894 
   Size:         29     656     209

  Fragments arranged by size: 

                 656     209      29
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     316     350     582     616     894 
   Size:        316      34     232      34     278

  Fragments arranged by size: 

                 316     278     232      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     158     894 
   Size:        158     736
 
BfrBI ATG'CAT

Cuts at:      0     603     894 
   Size:        603     291
 
Bme1580I G_kGCm'C

Cuts at:      0     228     894 
   Size:        228     666
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     419     894 
   Size:        419     475
 
BsaAI yAC'GTr

Cuts at:      0     199     894 
   Size:        199     695
 
BsaBI GATnn'nnATC

Cuts at:      0     128     894 
   Size:        128     766
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     518     894 
   Size:        518     376
 
BsiHKAI G_wGCw'C

Cuts at:      0     158     228     511     533     894 
   Size:        158      70     283      22     361

  Fragments arranged by size: 

                 361     283     158      70      22
 
Bsp1286I G_dGCh'C

Cuts at:      0     158     228     511     533     894 
   Size:        158      70     283      22     361

  Fragments arranged by size: 

                 361     283     158      70      22
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0      76     528     894 
   Size:         76     452     366

  Fragments arranged by size: 

                 452     366      76
 
Bsu36I CC'TnA_GG

Cuts at:      0     887     894 
   Size:        887       7
 
BtgI C'CryG_G

Cuts at:      0     212     894 
   Size:        212     682
 
DraI TTT'AAA

Cuts at:      0     182     639     894 
   Size:        182     457     255

  Fragments arranged by size: 

                 457     255     182
 
DraIII CAC_nnn'GTG

Cuts at:      0     528     894 
   Size:        528     366
 
EaeI y'GGCC_r

Cuts at:      0     209     894 
   Size:        209     685
 
EcoICRI GAG'CTC

Cuts at:      0     156     509     894 
   Size:        156     353     385

  Fragments arranged by size: 

                 385     353     156
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     217     419     460     894 
   Size:        217     202      41     434

  Fragments arranged by size: 

                 434     217     202      41
 
EcoO109I rG'GnC_Cy

Cuts at:      0     578     894 
   Size:        578     316
 
EcoRI G'AATT_C

Cuts at:      0     305     894 
   Size:        305     589
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     719     751     757     789     894 
   Size:        719      32       6      32     105

  Fragments arranged by size: 

                 719     105      32      32       6
 
HincII GTy'rAC

Cuts at:      0     776     894 
   Size:        776     118
 
HindIII A'AGCT_T

Cuts at:      0     101     894 
   Size:        101     793
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     282     894 
   Size:        282     612
 
NsiI A_TGCA'T

Cuts at:      0     605     894 
   Size:        605     289
 
NspI r_CATG'y

Cuts at:      0     625     894 
   Size:        625     269
 
PciI A'CATG_T

Cuts at:      0     621     894 
   Size:        621     273
 
PstI C_TGCA'G

Cuts at:      0     681     894 
   Size:        681     213
 
SacI G_AGCT'C

Cuts at:      0     158     511     894 
   Size:        158     353     383

  Fragments arranged by size: 

                 383     353     158
 
ScaI AGT'ACT

Cuts at:      0     473     894 
   Size:        473     421
 
SfcI C'TryA_G

Cuts at:      0     677     788     894 
   Size:        677     111     106

  Fragments arranged by size: 

                 677     111     106
 
TatI w'GTAC_w

Cuts at:      0     471     894 
   Size:        471     423
 

 Enzymes that do cut:

     AccI     AcuI   AflIII     AhdI    ApaLI     ApoI     BanI    BanII
     BbsI     BcgI    BciVI    BfrBI Bme1580I     BpmI    BsaAI    BsaBI
     BsgI  BsiHKAI Bsp1286I   BstAPI   Bsu36I     BtgI     DraI   DraIII
     EaeI  EcoICRI  Eco57MI EcoO109I    EcoRI    Hin4I   HincII  HindIII
     MmeI     NsiI     NspI     PciI     PstI     SacI     ScaI     SfcI
     TatI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII     AgeI     AleI
     AlfI     AloI    AlwNI     ApaI     AscI     AseI    AsiSI     AvaI
    AvrII     BaeI    BamHI     BbeI    BbvCI     BclI     BglI    BglII
     BlpI    BmgBI     BmrI     BmtI     BplI   Bpu10I    BpuEI     BsaI
    BsaHI    BsaWI    BsaXI    BseRI    BseYI    BsiEI    BsiWI     BsmI
    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI
   BssHII    BssSI    BstBI   BstEII    BstXI    BstYI  BstZ17I    BtgZI
     BtsI     ClaI    CspCI     DrdI     EagI     EarI     EciI    EcoNI
    EcoRV     FalI     FseI     FspI    FspAI    HaeII     HpaI     KasI
     KpnI     MfeI     MluI     MscI     MslI   MspA1I     NaeI     NarI
     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     PacI     PasI
    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PvuI    PvuII    RsrII    SacII     SalI
    SanDI     SapI     SbfI    SexAI     SfiI     SfoI     SgfI    SgrAI
     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI
     StyI     SwaI    TaqII     TstI  Tth111I     XbaI     XcmI     XhoI
     XmaI     XmnI     ZraI
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