Restriction map for pF1KB5484
##################################
# NO CUT ENZYMES (   96 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AflII       AgeI        Alw44I      ApaLI       AscI        
Asp700I     Asp718I     AvrII       BalI        BbsI        
BcgI        BciVI       BfaI        BfrI        BglII       
BlnI        BlpI        Bpu1102I    BpuAI       BsaBI       
BsaI        BsaWI       BseAI       BsiWI       BsmBI       
Bsp1407I    BspDI       BspEI       BspHI       BspLU11I    
BsrDI       BsrGI       Bst1107I    BstBI       BstZ17I     
CelII       ClaI        CpoI        DraI        DraIII      
EcoRI       EcoRV       EcoT22I     FseI        HincII      
HindII      HindIII     HpaI        KpnI        KspI        
MaeI        MamI        MfeI        MluI        MluNI       
MroI        MscI        MunI        NaeI        NdeI        
NgoMIV      NheI        NotI        NruI        NsiI        
NspV        PacI        PaeR7I      PinAI       PmeI        
PshAI       Psp1406I    PvuI        RcaI        RsrII       
SacI        SacII       SalI        SapI        SfiI        
SfuI        SgrAI       SmlI        SnaBI       SpeI        
SspBI       SspI        SwaI        XbaI        XhoI        
XmnI        


###################################
# ONE CUT ENZYMES (   48 enzymes) #
###################################

AcsI          430  GAATTT               [GA]^AATT[CT]
AcyI          182  GGCGCC               G[GA]^CG[CT]C
AflIII        315  ACGTGT               A^C[GA][CT]GT
AhdI         1006  GACAACCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI            4  GGGCCC               GGGCC^C
ApoI          430  GAATTT               [GA]^AATT[CT]
AseI          973  ATTAAT               AT^TAAT
AsnI          973  ATTAAT               AT^TAAT
AspEI        1006  GACAACCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI          245  GACGCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BamHI        1311  GGATCC               G^GATCC
BbeI          182  GGCGCC               GGCGC^C
BbrPI         314  CACGTG               CAC^GTG
BbvCI         718  GCTGAGG              CCTCAGC(-5/-2)
BclI         1757  TGATCA               T^GATCA
BsaAI         314  CACGTG               [CT]AC^GT[GA]
BsaHI         182  GGCGCC               G[GA]^CG[CT]C
BsgI          562  CTGCAC               GTGCAG(16/14)
BsmAI         251  GTCTC                GTCTC(1/5)
BsmI         1650  GAATGC               GAATGC(1/-1)
BssSI         919  CACGAG               CACGAG(-5/-1)
BstEII        670  GGTGACC              G^GT[ACGT]ACC
BstPI         670  GGTGACC              G^GT[ACGT]ACC
DrdI         1722  GACAGGGGTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I     1006  GACAACCAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI        1416  CCTGGCTCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I       670  GGTGACC              G^GT[ACGT]ACC
FbaI         1757  TGATCA               T^GATCA
Hin1I         182  GGCGCC               G[GA]^CG[CT]C
KasI          182  GGCGCC               G^GCGCC
MaeII         315  ACGT                 A^CGT
MseI          974  TTAA                 T^TAA
NarI          182  GGCGCC               GG^CGCC
PflFI         245  GACGCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PleI         1077  GACTC                GAGTC(4/5)
PmaCI         314  CACGTG               CAC^GTG
PmlI          314  CACGTG               CAC^GTG
PshBI         973  ATTAAT               AT^TAAT
SbfI         1194  CCTGCAGG             CCTGCA^GG
ScaI          278  AGTACT               AGT^ACT
SexAI         398  ACCAGGT              A^CC[AT]GGT
SfoI          182  GGCGCC               GGC^GCC
Sse8387I     1194  CCTGCAGG             CCTGCA^GG
StuI          536  AGGCCT               AGG^CCT
TaqI          243  TCGA                 T^CGA
Tru9I         974  TTAA                 T^TAA
Tth111I       245  GACGCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I       243  TCGA                 T^CGA


###################################
# TWO CUT ENZYMES (   29 enzymes) #
###################################

AfaI          279  GTAC                 GT^AC
              294  GTAC                
AocI          930  CCTGAGG              CC^T[ACGT]AGG
             1210  CCTCAGG             
Aor51HI       236  AGCGCT               AGC^GCT
              350  AGCGCT              
AspHI         916  GAGCAC               G[AT]GC[AT]^C
             1054  GAGCAC              
AviII         206  TGCGCA               TGC^GCA
             1808  TGCGCA              
BanII           4  GGGCCC               G[GA]GC[CT]^C
              129  GAGCCC              
BsiEI         140  CGGCCG               CG[GA][CT]^CG
              213  CGGCCG              
BsiHKAI       916  GAGCAC               G[AT]GC[AT]^C
             1054  GAGCAC              
BsrBI         372  GAGCGG               CCGCTC(-3/-3)
             1766  GAGCGG              
BssHII         93  GCGCGC               G^CGCGC
               95  GCGCGC              
BstAPI        264  GCAGCGCGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             1275  GCACACGCTGC         
BstYI        1203  AGATCC               [GA]^GATC[CT]
             1311  GGATCC              
Bsu36I        930  CCTGAGG              CC^T[ACGT]AGG
             1210  CCTCAGG             
EagI          140  CGGCCG               C^GGCCG
              213  CGGCCG              
EclXI         140  CGGCCG               C^GGCCG
              213  CGGCCG              
Eco47III      236  AGCGCT               AGC^GCT
              350  AGCGCT              
Eco52I        140  CGGCCG               C^GGCCG
              213  CGGCCG              
Eco81I        930  CCTGAGG              CC^T[ACGT]AGG
             1210  CCTCAGG             
FspI          206  TGCGCA               TGC^GCA
             1808  TGCGCA              
MaeIII        671  GTGAC                ^GT[ACGT]AC
              850  GTCAC               
MflI         1203  AGATCC               [GA]^GATC[CT]
             1311  GGATCC              
PflMI        1293  CCAGCATGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1839  CCATGGCGTGG         
PvuII         378  CAGCTG               CAG^CTG
             1715  CAGCTG              
RsaI          279  GTAC                 GT^AC
              294  GTAC                
SphI          575  GCATGC               GCATG^C
              896  GCATGC              
Tsp45I        671  GTGAC                ^GT[GC]AC
              850  GTCAC               
Tsp509I       431  AATT                 ^AATT
             1481  AATT                
Van91I       1293  CCAGCATGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1839  CCATGGCGTGG         
XhoII        1203  AGATCC               [GA]^GATC[CT]
             1311  GGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   85 enzymes) #
#########################################

AccII       AciI        AluI        AlwI        AlwNI       
AvaI        AvaII       BanI        BbvI        BcnI        
BglI        BmyI        BpmI        BsaJI       BseRI       
BsiYI       BslI        BsmFI       BsoBI       Bsp1286I    
BspMI       BsrFI       BsrI        BssKI       BstF5I      
BstNI       BstUI       BstXI       Cac8I       CfoI        
Cfr10I      Cfr13I      DdeI        DpnII       DraII       
DsaI        EaeI        EarI        EcoO109I    EcoRII      
EcoT14I     FauI        Fnu4HI      FokI        HaeII       
HaeIII      HapII       HgaI        HhaI        HinP1I      
HinfI       HpaII       HphI        ItaI        Ksp632I     
MboI        MboII       MnlI        MslI        MspA1I      
MspI        MvaI        MvnI        MwoI        NciI        
NcoI        NdeII       NlaIII      NlaIV       NspI        
PpuMI       PstI        Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        SmaI        StyI        TfiI        
TseI        TspRI       XcmI        XmaCI       XmaI        
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