Restriction map for pF1KB5484
 (Linear) (Six-base) MAPSORT of: KIBB5484.seq  Check: 5614  from: 1  to: 1950



KIBB5484.orf

 With 182 enzymes: SgfI * 

                        September 18, 2009 17:00  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1080    1950 
   Size:       1080     870
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     479     809    1950 
   Size:        479     330    1141

  Fragments arranged by size: 

                1141     479     330
 
AfeI AGC'GCT

Cuts at:      0     238     352    1950 
   Size:        238     114    1598

  Fragments arranged by size: 

                1598     238     114
 
AflIII A'CryG_T

Cuts at:      0     315    1950 
   Size:        315    1635
 
AhdI GACnn_n'nnGTC

Cuts at:      0    1011    1950 
   Size:       1011     939
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1047    1080    1113    1389    1428    1950 
   Size:       1047      33      33     276      39     522

  Fragments arranged by size: 

                1047     522     276      39      33      33
 
ApaI G_GGCC'C

Cuts at:      0       8    1950 
   Size:          8    1942
 
ApoI r'AATT_y

Cuts at:      0     430    1950 
   Size:        430    1520
 
AseI AT'TA_AT

Cuts at:      0     974    1950 
   Size:        974     976
 
AvaI C'yCGr_G

Cuts at:      0      42     150     570     696     861    1315    1950 
   Size:         42     108     420     126     165     454     635

  Fragments arranged by size: 

                 635     454     420     165     126     108      42
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     277     310    1950 
   Size:        277      33    1640

  Fragments arranged by size: 

                1640     277      33
 
BamHI G'GATC_C

Cuts at:      0    1311    1950 
   Size:       1311     639
 
BanI G'GyrC_C

Cuts at:      0     182     453    1710    1950 
   Size:        182     271    1257     240

  Fragments arranged by size: 

                1257     271     240     182
 
BanII G_rGCy'C

Cuts at:      0       8     133    1950 
   Size:          8     125    1817

  Fragments arranged by size: 

                1817     125       8
 
BbeI G_GCGC'C

Cuts at:      0     186    1950 
   Size:        186    1764
 
BbvCI CC'TCA_GC

Cuts at:      0     719    1950 
   Size:        719    1231
 
BclI T'GATC_A

Cuts at:      0    1757    1950 
   Size:       1757     193
 
BglI GCCn_nnn'nGGC

Cuts at:      0      44     166    1198    1950 
   Size:         44     122    1032     752

  Fragments arranged by size: 

                1032     752     122      44
 
Bme1580I G_kGCm'C

Cuts at:      0       8     410    1950 
   Size:          8     402    1540

  Fragments arranged by size: 

                1540     402       8
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1469    1950 
   Size:       1469     481
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1798    1950 
   Size:       1798     152
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     295     620     970    1102    1520    1894    1950 
   Size:        295     325     350     132     418     374      56

  Fragments arranged by size: 

                 418     374     350     325     295     132      56
 
Bpu10I CC'TnA_GC

Cuts at:      0     719    1421    1950 
   Size:        719     702     529

  Fragments arranged by size: 

                 719     702     529
 
BsaAI yAC'GTr

Cuts at:      0     316    1950 
   Size:        316    1634
 
BsaHI Gr'CG_yC

Cuts at:      0     183    1950 
   Size:        183    1767
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     236     266     340     370     607     637    1078    1108 
   Size:        236      30      74      30     237      30     441      30

Cuts at:   1108    1950 
   Size:        842

  Fragments arranged by size: 

                 842     441     237     236      74      30      30      30
                  30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     748     967    1099    1384    1441    1891    1950 
   Size:        748     219     132     285      57     450      59

  Fragments arranged by size: 

                 748     450     285     219     132      59      57
 
BseYI C'CCAG_C

Cuts at:      0     218    1717    1950 
   Size:        218    1499     233

  Fragments arranged by size: 

                1499     233     218
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     547    1950 
   Size:        547    1403
 
BsiEI CG_ry'CG

Cuts at:      0     143     216    1950 
   Size:        143      73    1734

  Fragments arranged by size: 

                1734     143      73
 
BsiHKAI G_wGCw'C

Cuts at:      0     920    1058    1950 
   Size:        920     138     892

  Fragments arranged by size: 

                 920     892     138
 
BsmI GAATG_Cn'

Cuts at:      0    1656    1950 
   Size:       1656     294
 
Bsp1286I G_dGCh'C

Cuts at:      0       8     133     410     920    1058    1950 
   Size:          8     125     277     510     138     892

  Fragments arranged by size: 

                 892     510     277     138     125       8
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     212    1031    1080    1256    1950 
   Size:        212     819      49     176     694

  Fragments arranged by size: 

                 819     694     212     176      49
 
BsrBI CCG'CTC

Cuts at:      0     374    1768    1950 
   Size:        374    1394     182

  Fragments arranged by size: 

                1394     374     182
 
BsrFI r'CCGG_y

Cuts at:      0     121     211     467    1950 
   Size:        121      90     256    1483

  Fragments arranged by size: 

                1483     256     121      90
 
BssHII G'CGCG_C

Cuts at:      0      93      95    1950 
   Size:         93       2    1855

  Fragments arranged by size: 

                1855      93       2
 
BssSI C'ACGA_G

Cuts at:      0     919    1950 
   Size:        919    1031
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     270    1281    1950 
   Size:        270    1011     669

  Fragments arranged by size: 

                1011     669     270
 
BstEII G'GTnAC_C

Cuts at:      0     670    1950 
   Size:        670    1280
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     169     345    1482    1950 
   Size:        169     176    1137     468

  Fragments arranged by size: 

                1137     468     176     169
 
BstYI r'GATC_y

Cuts at:      0    1203    1311    1950 
   Size:       1203     108     639

  Fragments arranged by size: 

                1203     639     108
 
Bsu36I CC'TnA_GG

Cuts at:      0     931    1211    1950 
   Size:        931     280     739

  Fragments arranged by size: 

                 931     739     280
 
BtgI C'CryG_G

Cuts at:      0     344     606     998    1307    1839    1950 
   Size:        344     262     392     309     532     111

  Fragments arranged by size: 

                 532     392     344     309     262     111
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1125    1950 
   Size:       1125     825
 
BtsI GCAGTG_nn'

Cuts at:      0     842    1950 
   Size:        842    1108
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    1728    1950 
   Size:       1728     222
 
EaeI y'GGCC_r

Cuts at:      0     119     140     213    1301    1950 
   Size:        119      21      73    1088     649

  Fragments arranged by size: 

                1088     649     119      73      21
 
EagI C'GGCC_G

Cuts at:      0     140     213    1950 
   Size:        140      73    1737

  Fragments arranged by size: 

                1737     140      73
 
EarI CTCTTCn'nnn_

Cuts at:      0     725     785     791    1950 
   Size:        725      60       6    1159

  Fragments arranged by size: 

                1159     725      60       6
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    1196    1231    1787    1950 
   Size:       1196      35     556     163

  Fragments arranged by size: 

                1196     556     163      35
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     295     479     620     809     970    1102    1520    1894 
   Size:        295     184     141     189     161     132     418     374

Cuts at:   1894    1950 
   Size:         56

  Fragments arranged by size: 

                 418     374     295     189     184     161     141     132
                  56
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1420    1950 
   Size:       1420     530
 
EcoO109I rG'GnC_Cy

Cuts at:      0       4     147     201     258     444     552     641    1044 
   Size:          4     143      54      57     186     108      89     403

Cuts at:   1044    1471    1950 
   Size:        427     479

  Fragments arranged by size: 

                 479     427     403     186     143     108      89      57
                  54       4
 
FspI TGC'GCA

Cuts at:      0     208    1810    1950 
   Size:        208    1602     140

  Fragments arranged by size: 

                1602     208     140
 
HaeII r_GCGC'y

Cuts at:      0     186     240     354    1113    1164    1245    1950 
   Size:        186      54     114     759      51      81     705

  Fragments arranged by size: 

                 759     705     186     114      81      54      51
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     236     268     724     756     997    1029    1078    1110 
   Size:        236      32     456      32     241      32      49      32

Cuts at:   1110    1365    1397    1582    1614    1729    1761    1950 
   Size:        255      32     185      32     115      32     189

  Fragments arranged by size: 

                 456     255     241     236     189     185     115      49
                  32      32      32      32      32      32      32
 
KasI G'GCGC_C

Cuts at:      0     182    1950 
   Size:        182    1768
 
MslI CAynn'nnrTG

Cuts at:      0     343    1558    1910    1950 
   Size:        343    1215     352      40

  Fragments arranged by size: 

                1215     352     343      40
 
MspA1I CmG'CkG

Cuts at:      0      19      57      63     218     380     416    1626    1717 
   Size:         19      38       6     155     162      36    1210      91

Cuts at:   1717    1902    1950 
   Size:        185      48

  Fragments arranged by size: 

                1210     185     162     155      91      48      38      36
                  19       6
 
NarI GG'CG_CC

Cuts at:      0     183    1950 
   Size:        183    1767
 
NcoI C'CATG_G

Cuts at:      0     344     998    1839    1950 
   Size:        344     654     841     111

  Fragments arranged by size: 

                 841     654     344     111
 
NspI r_CATG'y

Cuts at:      0     579     900    1300    1950 
   Size:        579     321     400     650

  Fragments arranged by size: 

                 650     579     400     321
 
PasI CC'CwG_GG

Cuts at:      0     549    1498    1950 
   Size:        549     949     452

  Fragments arranged by size: 

                 949     549     452
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1299    1845    1950 
   Size:       1299     546     105

  Fragments arranged by size: 

                1299     546     105
 
PfoI T'CCnGG_A

Cuts at:      0     254     367    1442    1934    1950 
   Size:        254     113    1075     492      16

  Fragments arranged by size: 

                1075     492     254     113      16
 
PmlI CAC'GTG

Cuts at:      0     316    1950 
   Size:        316    1634
 
PpuMI rG'GwC_Cy

Cuts at:      0     201     258     641    1471    1950 
   Size:        201      57     383     830     479

  Fragments arranged by size: 

                 830     479     383     201      57
 
PspOMI G'GGCC_C

Cuts at:      0       4    1950 
   Size:          4    1946
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0    1909    1941    1950 
   Size:       1909      32       9

  Fragments arranged by size: 

                1909      32       9
 
PstI C_TGCA'G

Cuts at:      0     286     308     544    1076    1199    1532    1901    1950 
   Size:        286      22     236     532     123     333     369      49

  Fragments arranged by size: 

                 532     369     333     286     236     123      49      22
            
 
PvuII CAG'CTG

Cuts at:      0     380    1717    1950 
   Size:        380    1337     233

  Fragments arranged by size: 

                1337     380     233
 
SanDI GG'GwC_CC

Cuts at:      0     258     641    1471    1950 
   Size:        258     383     830     479

  Fragments arranged by size: 

                 830     479     383     258
 
SbfI CC_TGCA'GG

Cuts at:      0    1199    1950 
   Size:       1199     751
 
ScaI AGT'ACT

Cuts at:      0     280    1950 
   Size:        280    1670
 
SexAI A'CCwGG_T

Cuts at:      0     398    1950 
   Size:        398    1552
 
SfcI C'TryA_G

Cuts at:      0     282     304     540    1072    1195    1528    1897    1950 
   Size:        282      22     236     532     123     333     369      53

  Fragments arranged by size: 

                 532     369     333     282     236     123      53      22
            
 
SfoI GGC'GCC

Cuts at:      0     184    1950 
   Size:        184    1766
 
SmaI CCC'GGG

Cuts at:      0      44     152     698    1950 
   Size:         44     108     546    1252

  Fragments arranged by size: 

                1252     546     108      44
 
SphI G_CATG'C

Cuts at:      0     579     900    1950 
   Size:        579     321    1050

  Fragments arranged by size: 

                1050     579     321
 
SrfI GCCC'GGGC

Cuts at:      0      44    1950 
   Size:         44    1906
 
StuI AGG'CCT

Cuts at:      0     538    1950 
   Size:        538    1412
 
StyI C'CwwG_G

Cuts at:      0     344     998    1172    1544    1663    1839    1950 
   Size:        344     654     174     372     119     176     111

  Fragments arranged by size: 

                 654     372     344     176     174     119     111
 
TatI w'GTAC_w

Cuts at:      0     278    1950 
   Size:        278    1672
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     340     372     607     639    1048    1080    1950 
   Size:        340      32     235      32     409      32     870

  Fragments arranged by size: 

                 870     409     340     235      32      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     248    1950 
   Size:        248    1702
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     114     876     945    1869    1950 
   Size:        114     762      69     924      81

  Fragments arranged by size: 

                 924     762     114      81      69
 
XmaI C'CCGG_G

Cuts at:      0      42     150     696    1950 
   Size:         42     108     546    1254

  Fragments arranged by size: 

                1254     546     108      42
 

 Enzymes that do cut:

     AarI     AcuI     AfeI   AflIII     AhdI    AlwNI     ApaI     ApoI
     AseI     AvaI     BaeI    BamHI     BanI    BanII     BbeI    BbvCI
     BclI     BglI Bme1580I     BmrI     BplI     BpmI   Bpu10I    BsaAI
    BsaHI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI
 Bsp1286I    BspMI    BsrBI    BsrFI   BssHII    BssSI   BstAPI   BstEII
    BstXI    BstYI   Bsu36I     BtgI    BtgZI     BtsI     DrdI     EaeI
     EagI     EarI     EciI  Eco57MI    EcoNI EcoO109I     FspI    HaeII
    Hin4I     KasI     MslI   MspA1I     NarI     NcoI     NspI     PasI
    PflMI     PfoI     PmlI    PpuMI   PspOMI     PsrI     PstI    PvuII
    SanDI     SbfI     ScaI    SexAI     SfcI     SfoI     SmaI     SphI
     SrfI     StuI     StyI     TatI     TstI  Tth111I     XcmI     XmaI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI    AflII     AgeI     AleI     AlfI
     AloI    ApaLI     AscI    AsiSI    AvrII     BbsI     BcgI    BciVI
    BfrBI    BglII     BlpI    BmgBI     BmtI    BpuEI     BsaI    BsaBI
    BsaWI    BsiWI    BsmBI    BspEI    BspHI    BsrDI    BsrGI    BstBI
  BstZ17I     ClaI    CspCI     DraI   DraIII  EcoICRI    EcoRI    EcoRV
     FalI     FseI    FspAI   HincII  HindIII     HpaI     KpnI     MfeI
     MluI     MmeI     MscI     NaeI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     PacI     PciI     PmeI     PpiI    PshAI     PsiI
    PspXI     PvuI    RsrII     SacI    SacII     SalI     SapI     SfiI
     SgfI    SgrAI     SmlI    SnaBI     SpeI     SspI     SwaI    TaqII
     XbaI     XhoI     XmnI     ZraI
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