Restriction map for pF1KB5463
##################################
# NO CUT ENZYMES (  138 enzymes) #
##################################

AatII       Acc65I      AclI        AcsI        AcyI        
AflII       AgeI        AhdI        Alw44I      ApaI        
ApaLI       ApoI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AspI        AvaI        AviII       
AvrII       BanI        BbeI        BbrPI       BbvCI       
BciVI       BcnI        BfaI        BfrI        BglII       
BlnI        BlpI        Bpu1102I    BsaAI       BsaHI       
BsgI        BsiEI       BsiWI       BsmFI       BsmI        
BsoBI       Bsp1407I    BspDI       BsrDI       BsrGI       
Bst1107I    BstAPI      BstBI       BstEII      BstPI       
BstZ17I     CelII       ClaI        CpoI        DraI        
DraII       DraIII      DrdI        EagI        Eam1105I    
EarI        EclXI       Eco52I      EcoNI       EcoO109I    
EcoO65I     EcoRI       EcoRV       EcoT22I     FseI        
FspI        Hin1I       HincII      HindII      HindIII     
HpaI        KasI        KpnI        Ksp632I     KspI        
MaeI        MfeI        MluI        MunI        NaeI        
NarI        NciI        NcoI        NdeI        NgoMIV      
NheI        NotI        NruI        NsiI        NspV        
PacI        PaeR7I      PflFI       PflMI       PinAI       
PmaCI       PmeI        PmlI        PpuMI       PshAI       
PshBI       Psp1406I    PstI        PvuI        RsrII       
SacII       SalI        SapI        SbfI        ScaI        
SexAI       SfcI        SfiI        SfoI        SfuI        
SgrAI       SmaI        SmlI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        SwaI        
Tsp509I     Tth111I     Van91I      XbaI        XhoI        
XmaCI       XmaI        XmnI        


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# ONE CUT ENZYMES (   61 enzymes) #
###################################

AccI          721  GTAGAC               GT^[AC][GT]AC
AccIII        344  TCCGGA               T^CCGGA
AflIII        641  ACATGT               A^C[GA][CT]GT
AlwNI         568  CAGGCTCTG            CAG[ACGT][ACGT][ACGT]^CTG
AocI          336  CCTGAGG              CC^T[ACGT]AGG
Aor51HI       428  AGCGCT               AGC^GCT
AscI          780  GGCGCGCC             GG^CGCGCC
AspHI          19  GAGCTC               G[AT]GC[AT]^C
BalI          656  TGGCCA               TGG^CCA
BamHI         341  GGATCC               G^GATCC
BanII          19  GAGCTC               G[GA]GC[CT]^C
BcgI          827  GCATCGTCATCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI          800  TGATCA               T^GATCA
BglI          178  GCCATCCTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BmyI           19  GAGCTC               G[AGT]GC[ACT]^C
BsaBI         801  GATCATCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI           77  GAGACC               GGTCTC(1/5)
BsaWI         344  TCCGGA               [AT]^CCGG[AT]
BseAI         344  TCCGGA               T^CCGGA
BsiHKAI        19  GAGCTC               G[AT]GC[AT]^C
BsmBI         586  GAGACG               CGTCTC(1/5)
Bsp1286I       19  GAGCTC               G[AGT]GC[ACT]^C
BspEI         344  TCCGGA               T^CCGGA
BspHI         797  TCATGA               T^CATGA
BspLU11I      641  ACATGT               A^CATGT
BspMI         105  GCAGGT               ACCTGC(4/8)
BsrBI         321  CCGCTC               CCGCTC(-3/-3)
BsrFI         466  ACCGGC               [GA]^CCGG[CT]
BsrI          482  CCAGT                ACTGG(1/-1)
BssHII        781  GCGCGC               G^CGCGC
BssSI         664  CTCGTG               CACGAG(-5/-1)
BstXI         659  CCATGCTCGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI         341  GGATCC               [GA]^GATC[CT]
Bsu36I        336  CCTGAGG              CC^T[ACGT]AGG
Cfr10I        466  ACCGGC               [GA]^CCGG[CT]
DsaI           68  CCGTGG               C^C[GA][CT]GG
Eco47III      428  AGCGCT               AGC^GCT
Eco81I        336  CCTGAGG              CC^T[ACGT]AGG
EcoT14I        32  CCAAGG               C^C[AT][AT]GG
FauI          520  CCCGC                CCCGC(4/6)
FbaI          800  TGATCA               T^GATCA
HaeII         428  AGCGCT               [GA]GCGC^[CT]
HgaI          588  GACGC                GACGC(5/10)
MaeII         149  ACGT                 A^CGT
MaeIII         64  GTCAC                ^GT[ACGT]AC
MamI          801  GATCATCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI          341  GGATCC               [GA]^GATC[CT]
MluNI         656  TGGCCA               TGG^CCA
MroI          344  TCCGGA               T^CCGGA
MscI          656  TGGCCA               TGG^CCA
MseI          277  TTAA                 T^TAA
PleI          550  GACTC                GAGTC(4/5)
PvuII         454  CAGCTG               CAG^CTG
RcaI          797  TCATGA               T^CATGA
SacI           19  GAGCTC               GAGCT^C
StuI          312  AGGCCT               AGG^CCT
StyI           32  CCAAGG               C^C[AT][AT]GG
TfiI          117  GATTC                G^A[AT]TC
Tru9I         277  TTAA                 T^TAA
Tsp45I         64  GTCAC                ^GT[GC]AC
XhoII         341  GGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   10 enzymes) #
###################################

BbvI          453  GCAGC                GCAGC(8/12)
              431  GCTGC               
BsmAI          77  GAGAC                GTCTC(1/5)
              586  GAGAC               
DdeI          337  CTGAG                C^T[ACGT]AG
              574  CTGAG               
EaeI           29  CGGCCA               [CT]^GGCC[GA]
              656  TGGCCA              
HinfI         117  GATTC                G^A[ACGT]TC
              550  GACTC               
MspA1I        329  CCGCTG               C[AC]G^C[GT]G
              454  CAGCTG              
NlaIV         341  GGATCC               GG[ACGT]^[ACGT]CC
              515  GGAACC              
NspI          500  ACATGC               [GA]CATG^[CT]
              641  ACATGT              
TseI          431  GCTGC                G^C[AT]GC
              453  GCAGC               
XcmI          371  CCAGAAAGTTTGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
              482  CCAGTGAGGAGCTGG     


#########################################
# 3 OR MORE CUT ENZYMES (   49 enzymes) #
#########################################

AccII       AciI        AfaI        AluI        AlwI        
AvaII       BbsI        BpmI        BpuAI       BsaJI       
BseRI       BsiYI       BslI        BssKI       BstF5I      
BstNI       BstUI       Cac8I       CfoI        Cfr13I      
DpnII       EcoRII      Fnu4HI      FokI        HaeIII      
HapII       HhaI        HinP1I      HpaII       HphI        
ItaI        MboI        MboII       MnlI        MslI        
MspI        MvaI        MvnI        MwoI        NdeII       
NlaIII      RsaI        Sau3AI      Sau96I      ScrFI       
SfaNI       TaqI        TspRI       TthHB8I     
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